Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	2,602,017	(C)7→10	coding (200/2019 nt)	hyfB →	hydrogenase 4, membrane subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2602010	4 (0.080)	59 (1.100) +CCC	32/200	0.4	93.6%	coding (193/2019 nt)	hyfB	hydrogenase 4, membrane subunit
	?	NC_000913	2602011 =	4 (0.070)					coding (194/2019 nt)	hyfB	hydrogenase 4, membrane subunit

TGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2601873‑2602010 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGG  >  NC_000913/2602011‑2602139                                                                                                                                           |||                                                                                                                                  TGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC                                                                                                                                     >  2:872759/1‑139   GCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCC                                                                                                                                   >  2:253051/1‑139      TCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC                                                                                                                                <  1:709016/139‑1           GTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCCtt                                                                                                                           <  2:399658/139‑3                                ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCCCTTTTGAATTTGCCGATTTAACC                                                                                                      >  2:809847/1‑139                                ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCC                                                                                                     <  1:1105855/139‑1                                ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCC                                                                                                     >  2:1146120/1‑139                                ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCC                                                                                                     >  1:116976/1‑139                                ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCC                                                                                                     <  2:966745/139‑1                                ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCC                                                                                                     >  1:966745/1‑139                                ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCC                                                                                                     >  2:1105855/1‑139                                ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCC                                                                                                     >  2:653470/1‑139                                 CTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC                                                                                                                                >  2:447124/1‑112                                 CTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC                                                                                                                                <  1:447124/112‑1                                       ATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAA                                                                                               <  1:809847/139‑1                                              TTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGC                                                                                       <  2:781611/139‑1                                                  CGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCT                                                                                   <  1:1146120/139‑1                                                       TCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCA                                                                              >  2:455088/1‑139                                                              GCTGGTGGGCGGCGTTATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGG                                                                       <  1:1020752/139‑1                                                               CTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC                                                                                                                                <  2:764835/82‑1                                                               CTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC                                                                                                                                >  1:764835/1‑82                                                                  GTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCT                                                                   >  1:247616/1‑139                                                                  GTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑TCCCCCCCCCTTTTGAATTTTCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCT                                                                   >  2:276276/1‑139                                                                       CGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTA                                                              >  1:179415/1‑139                                                                       CGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGC                                                                                                                >  2:1080245/1‑89                                                                       CGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGC                                                                                                                <  1:1080245/89‑1                                                                       CGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTT                                                                                                                  >  2:640949/1‑87                                                                       CGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTT                                                                                                                  <  1:640949/87‑1                                                                                          AGCGGTATTACGCAATTACATGCTGGTGTAACTTTAGTCGGCCGGTTTG‑CCCCCCCCCTTTTGTATTTCCCGATTTAACCCTGCGAATGGATCGCCTCTCGGCATATATGGTGCTGGTTATCTCCTTGCTGGTGGGGGT                                           <  2:116976/139‑1                                                                                            CGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC                                                                                                                                >  1:679206/1‑53                                                                                            CGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC                                                                                                                                <  2:679206/53‑1                                                                                            CGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCC                                                                                                                                 <  1:1173713/52‑1                                                                                            CGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCC                                                                                                                                 >  2:1173713/1‑52                                                                                            CGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGG                                            >  2:434986/1‑136                                                                                            CGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGG                                            <  1:434986/136‑1                                                                                             GGAATTACGCAATTAAATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTG                                        <  1:455088/139‑1                                                                                                ATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTC                                     <  1:456267/139‑1                                                                                                ATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGC                                                                               >  2:512539/1‑97                                                                                                ATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGC                                                                               <  1:512539/97‑1                                                                                                 TTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCT                                    <  2:247616/139‑1                                                                                                          TACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCA                           <  1:749974/139‑1                                                                                                               GCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGAC                      <  1:653470/139‑1                                                                                                               GCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACC                                                                                                      >  2:364652/1‑59                                                                                                               GCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACC                                                                                                      <  1:364652/59‑1                                                                                                                 TGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTT                    >  2:275327/1‑139                                                                                                                 TGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTG                                                <  2:67061/111‑1                                                                                                                 TGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTG                                                >  1:67061/1‑111                                                                                                                 TGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTC                                                                                  >  2:218428/1‑77                                                                                                                 TGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTC                                                                                  <  1:218428/77‑1                                                                                                                   GTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTAT                  >  1:1034602/1‑139                                                                                                                   GTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTAT                  >  2:1078301/1‑139                                                                                                                   GTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGC                                                                               >  2:431144/1‑78                                                                                                                   GTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGC                                                                               <  1:431144/78‑1                                                                                                                   GTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCG                                                                                                 <  2:1228335/60‑1                                                                                                                   GTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCG                                                                                                 >  1:1228335/1‑60                                                                                                                             AGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAAT        >  2:981988/1‑139                                                                                                                               TCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAC      <  1:981988/139‑1                                                                                                                               TCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAC      <  1:253051/139‑1                                                                                                                                CGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACG     <  1:276276/139‑1                                                                                                                                    CGTTTTGCCCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATTCGCGAATACGAGG  >  2:640841/1‑139                                                                                                                                           |||                                                                                                                                  TGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2601873‑2602010 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGG  >  NC_000913/2602011‑2602139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.