New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 4281663	89 (1.010)	10 (0.120)	10/260	10.0	10.7%	intergenic (‑154/+24)	yjcF/actP	pentapeptide repeats protein/acetate transporter
	?	NC_000913_3_KO_pgi	= 4281667	85 (1.040)					intergenic (‑158/+20)	yjcF/actP	pentapeptide repeats protein/acetate transporter
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGA  >  NC_000913_3_KO_pgi/4281516‑4281811                                                                                                                                                    |                                                                                                                                                      tCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAg                                                                                                                                                     <  1:636280/150‑1 (MQ=255)        aaTTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCg                                                                                                                                             >  1:247163/1‑150 (MQ=255)         aTTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCgg                                                                                                                                            <  1:89427/150‑1 (MQ=255)          ttAACAAGAAAGAAACGGACGTGTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCggg                                                                                                                                           <  3:299542/150‑1 (MQ=255)          ttAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCggg                                                                                                                                           <  3:339019/150‑1 (MQ=255)             aCAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGaa                                                                                                                                        >  2:560991/1‑150 (MQ=255)              cAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATTGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAg                                                                                                                                       <  3:578493/150‑1 (MQ=255)              cAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAg                                                                                                                                       <  4:536426/150‑1 (MQ=255)                agaaagaaAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAggg                                                                                                                                     >  3:90319/1‑150 (MQ=255)                agaaagaaAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAggg                                                                                                                                     >  1:150821/1‑150 (MQ=255)                  aaagaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGga                                                                                                                                   <  3:142319/150‑1 (MQ=255)                     gaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGgagag                                                                                                                                <  4:268224/150‑1 (MQ=255)                     gaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGgagag                                                                                                                                <  4:268261/150‑1 (MQ=255)                      aaaaGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGgagaga                                                                                                                               <  2:827353/150‑1 (MQ=255)                          ggCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTaa                                                                                                                           <  1:560991/150‑1 (MQ=255)                           gCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAAt                                                                                                                          <  4:323286/150‑1 (MQ=255)                           gCATATTATCTGCATTATCATTTTCTACGACTGTCAAAAAGCGCTCATTTTTTAATGAGATTATTTTGTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAAAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAAt                                                                                                                          <  4:77580/150‑1 (MQ=255)                               atTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATgcgc                                                                                                                      >  2:291573/1‑150 (MQ=255)                                ttCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATgcgcg                                                                                                                     >  4:211120/1‑150 (MQ=255)                                   tctGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTaa                                                                                                                           >  4:420093/1‑141 (MQ=255)                                   tctGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTaa                                                                                                                           <  3:420093/141‑1 (MQ=255)                                       cATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCtt                                                                                                              >  1:679758/1‑150 (MQ=255)                                        aTTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATGTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTg                                                                                                             <  4:353671/150‑1 (MQ=255)                                         ttATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAga                                                                                                                                                    <  3:454603/111‑1 (MQ=255)                                         ttATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAga                                                                                                                                                    >  4:454603/1‑111 (MQ=255)                                         ttATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGc                                                                                                            >  1:107588/1‑150 (MQ=255)                                          tATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGCGCTTAATGCGCGCGGCCTTGCt                                                                                                           >  2:76616/1‑150 (MQ=255)                                           aTCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAgac                                                                                                                                                   <  2:781436/110‑1 (MQ=255)                                           aTCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAgac                                                                                                                                                   >  1:781436/1‑110 (MQ=255)                                              aTTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAAc                                                                                                       <  4:242395/150‑1 (MQ=255)                                                     gcGACTGTCAAAAATCGCTCATTTTGTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAg                                                                                                <  2:475452/149‑1 (MQ=255)                                                       gACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGcc                                                                                              >  1:651666/1‑150 (MQ=255)                                                          tGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGt                                                                                           <  3:235859/150‑1 (MQ=255)                                                              aaaaaTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTgg                                                                                       <  4:355353/150‑1 (MQ=255)                                                              aaaaaTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTgg                                                                                       >  3:16899/1‑150 (MQ=255)                                                              aaaaaTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTgg                                                                                       <  3:142641/150‑1 (MQ=255)                                                                  aTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGc                                                                                   <  2:651666/150‑1 (MQ=255)                                                                  aTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGc                                                                                   >  1:900483/1‑150 (MQ=255)                                                                  aTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGc                                                                                   <  2:188220/150‑1 (MQ=255)                                                                    cGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCgg                                                                                 >  2:794888/1‑150 (MQ=255)                                                                     gCTCATTTTTTAATGAGTTTATTTGTTTAATATTAGGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGa                                                                                <  4:542560/150‑1 (MQ=255)                                                                       tCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATa                                                                              >  3:240639/1‑150 (MQ=255)                                                                         aTTTTTTAATGCGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGCGCGGATaaa                                                                            <  2:318717/150‑1 (MQ=255)                                                                         aTTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATaaa                                                                            >  3:529579/1‑150 (MQ=255)                                                                         aTTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATaaa                                                                            <  3:211120/150‑1 (MQ=255)                                                                           tttttAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTC‑GTCTGACCGGGGaata                                                                                                                                      >  3:42873/1‑88 (MQ=38)                                                                             tttAATGAGTTTATATGTTTAATCTTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTgc                                                                        <  2:909477/150‑1 (MQ=255)                                                                              ttAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTgcg                                                                       <  4:156020/150‑1 (MQ=255)                                                                               tAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCg                                                                                  <  2:468951/138‑1 (MQ=255)                                                                               tAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGACTTGCTCAACGCCAAAGCCGGTCTGGGAGCg                                                                                  >  1:468951/1‑138 (MQ=255)                                                                                aaTGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATgcgcg                                                                                                                     <  2:98322/102‑1 (MQ=255)                                                                                aaTGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATgcgcg                                                                                                                     >  1:98322/1‑102 (MQ=255)                                                                                     gTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGaa                                                                >  4:97404/1‑150 (MQ=255)                                                                                     gTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGaa                                                                <  1:120716/150‑1 (MQ=255)                                                                                      tttATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACg                                                                                                      <  2:267558/111‑1 (MQ=255)                                                                                      tttATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACg                                                                                                      >  1:267558/1‑111 (MQ=255)                                                                                      tttATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACt                                                                          <  3:110532/139‑1 (MQ=255)                                                                                      tttATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACt                                                                          >  4:110532/1‑139 (MQ=255)                                                                                                 aaTATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacgctc                                                    <  4:529579/150‑1 (MQ=255)                                                                                                    attatGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacgcccacg                                                 >  4:465408/1‑150 (MQ=255)                                                                                                        tGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCg                                                                                             <  3:333670/102‑1 (MQ=255)                                                                                                        tGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCg                                                                                             >  4:333670/1‑102 (MQ=255)                                                                                                        tGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCAcgcgcg                                             >  1:564020/1‑150 (MQ=255)                                                                                                         gggAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACgcgcgc                                            >  2:774996/1‑150 (MQ=255)                                                                                                             aaaGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTc                                        <  4:249427/150‑1 (MQ=255)                                                                                                             aaaGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAAGGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGTAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTc                                        <  2:507392/150‑1 (MQ=255)                                                                                                               aGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCg                                      >  2:755363/1‑150 (MQ=255)                                                                                                                   gATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGa                                  <  3:83999/150‑1 (MQ=255)                                                                                                                   gATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGa                                  <  4:138254/150‑1 (MQ=255)                                                                                                                    aTGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAg                                 >  3:303532/1‑150 (MQ=255)                                                                                                                        aTTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTAt                             >  2:882808/1‑150 (MQ=255)                                                                                                                         ttcgggAGAGGAAGAGGCTTCCACGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGGGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGGACAGTTCACGCTCCCGAGCGCCTTCCGCTGGGTCATc                            >  2:417388/4‑150 (MQ=255)                                                                                                                            gggAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGt                         <  1:794888/150‑1 (MQ=255)                                                                                                                            gggAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGt                         >  1:373732/1‑150 (MQ=255)                                                                                                                             ggAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGCTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGtt                        <  4:468024/150‑1 (MQ=255)                                                                                                                                gagGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGcc                     <  4:170276/150‑1 (MQ=255)                                                                                                                                 agGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCg                    <  1:253859/150‑1 (MQ=255)                                                                                                                                   gAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtc                                                          <  1:838409/110‑1 (MQ=255)                                                                                                                                   gAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtc                                                          >  2:838409/1‑110 (MQ=255)                                                                                                                                   gAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCgag                  >  1:53444/1‑150 (MQ=255)                                                                                                                                     agagTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAAGGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTCCCGCTGACCTATCGGTTGCCGacaa                >  2:56442/1‑150 (MQ=255)                                                                                                                                       agTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGagaaga              <  1:156564/150‑1 (MQ=255)                                                                                                                                        cccttCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAAc                                                                                                       >  4:76503/4‑60 (MQ=255)                                                                                                                                        cccttCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAAc                                                                                                       <  3:76503/57‑1 (MQ=255)                                                                                                                                         tATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAAc            >  4:578600/1‑150 (MQ=255)                                                                                                                                           ttCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCa          <  1:291573/150‑1 (MQ=255)                                                                                                                                              cccGGTCAGAC‑GACCGGGGCAGGGGAGAGATTAATGCGCGCGTCCTTGCTCAACGCCTAAGCCGGTCTGGGAGTGTATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGCGTTATCGGTTGCCGAGAAGAACCAGAt       >  2:714642/1‑150 (MQ=255)                                                                                                                                              cccGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACt                                                                          <  4:432257/83‑1 (MQ=255)                                                                                                                                              cccGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACt                                                                          >  3:432257/1‑83 (MQ=255)                                                                                                                                                   tCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGa  <  2:321569/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      TCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGA  >  NC_000913_3_KO_pgi/4281516‑4281811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.