New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 4281663	89 (1.010)	10 (0.120)	10/260	10.0	10.7%	intergenic (‑154/+24)	yjcF/actP	pentapeptide repeats protein/acetate transporter
	?	NC_000913_3_KO_pgi	= 4281667	85 (1.040)					intergenic (‑158/+20)	yjcF/actP	pentapeptide repeats protein/acetate transporter
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAAT  >  NC_000913_3_KO_pgi/4281523‑4281816                                                                                                                                                 |                                                                                                                                                       aaTTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCg                                                                                                                                                  >  1:247163/1‑150 (MQ=255)  aTTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCgg                                                                                                                                                 <  1:89427/150‑1 (MQ=255)   ttAACAAGAAAGAAACGGACGTGTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCggg                                                                                                                                                <  3:299542/150‑1 (MQ=255)   ttAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCggg                                                                                                                                                <  3:339019/150‑1 (MQ=255)      aCAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGaa                                                                                                                                             >  2:560991/1‑150 (MQ=255)       cAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATTGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAg                                                                                                                                            <  3:578493/150‑1 (MQ=255)       cAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAg                                                                                                                                            <  4:536426/150‑1 (MQ=255)         agaaagaaAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAggg                                                                                                                                          >  1:150821/1‑150 (MQ=255)         agaaagaaAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAggg                                                                                                                                          >  3:90319/1‑150 (MQ=255)           aaagaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGga                                                                                                                                        <  3:142319/150‑1 (MQ=255)              gaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGgagag                                                                                                                                     <  4:268224/150‑1 (MQ=255)              gaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGgagag                                                                                                                                     <  4:268261/150‑1 (MQ=255)               aaaaGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGgagaga                                                                                                                                    <  2:827353/150‑1 (MQ=255)                   ggCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTaa                                                                                                                                <  1:560991/150‑1 (MQ=255)                    gCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAAt                                                                                                                               <  4:323286/150‑1 (MQ=255)                    gCATATTATCTGCATTATCATTTTCTACGACTGTCAAAAAGCGCTCATTTTTTAATGAGATTATTTTGTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAAAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAAt                                                                                                                               <  4:77580/150‑1 (MQ=255)                        atTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATgcgc                                                                                                                           >  2:291573/1‑150 (MQ=255)                         ttCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATgcgcg                                                                                                                          >  4:211120/1‑150 (MQ=255)                            tctGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTaa                                                                                                                                <  3:420093/141‑1 (MQ=255)                            tctGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTaa                                                                                                                                >  4:420093/1‑141 (MQ=255)                                cATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCtt                                                                                                                   >  1:679758/1‑150 (MQ=255)                                 aTTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATGTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTg                                                                                                                  <  4:353671/150‑1 (MQ=255)                                  ttATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGc                                                                                                                 >  1:107588/1‑150 (MQ=255)                                   tATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGCGCTTAATGCGCGCGGCCTTGCt                                                                                                                >  2:76616/1‑150 (MQ=255)                                    aTCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAgac                                                                                                                                                        <  2:781436/110‑1 (MQ=255)                                    aTCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAgac                                                                                                                                                        >  1:781436/1‑110 (MQ=255)                                       aTTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAAc                                                                                                            <  4:242395/150‑1 (MQ=255)                                              gcGACTGTCAAAAATCGCTCATTTTGTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAg                                                                                                     <  2:475452/149‑1 (MQ=255)                                                gACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGcc                                                                                                   >  1:651666/1‑150 (MQ=255)                                                   tGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGt                                                                                                <  3:235859/150‑1 (MQ=255)                                                       aaaaaTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTgg                                                                                            <  3:142641/150‑1 (MQ=255)                                                       aaaaaTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTgg                                                                                            >  3:16899/1‑150 (MQ=255)                                                       aaaaaTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTgg                                                                                            <  4:355353/150‑1 (MQ=255)                                                           aTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGc                                                                                        >  1:900483/1‑150 (MQ=255)                                                           aTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGc                                                                                        <  2:188220/150‑1 (MQ=255)                                                           aTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGc                                                                                        <  2:651666/150‑1 (MQ=255)                                                             cGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCgg                                                                                      >  2:794888/1‑150 (MQ=255)                                                              gCTCATTTTTTAATGAGTTTATTTGTTTAATATTAGGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGa                                                                                     <  4:542560/150‑1 (MQ=255)                                                                tCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATa                                                                                   >  3:240639/1‑150 (MQ=255)                                                                  aTTTTTTAATGCGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGCGCGGATaaa                                                                                 <  2:318717/150‑1 (MQ=255)                                                                  aTTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATaaa                                                                                 <  3:211120/150‑1 (MQ=255)                                                                  aTTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATaaa                                                                                 >  3:529579/1‑150 (MQ=255)                                                                    tttttAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTC‑GTCTGACCGGGGaata                                                                                                                                           >  3:42873/1‑88 (MQ=38)                                                                      tttAATGAGTTTATATGTTTAATCTTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAGGCCGGTCTGGGAGCGGATAAACTgc                                                                             <  2:909477/150‑1 (MQ=255)                                                                       ttAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTgcg                                                                            <  4:156020/150‑1 (MQ=255)                                                                        tAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCg                                                                                       <  2:468951/138‑1 (MQ=255)                                                                        tAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGACTTGCTCAACGCCAAAGCCGGTCTGGGAGCg                                                                                       >  1:468951/1‑138 (MQ=255)                                                                         aaTGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATgcgcg                                                                                                                          >  1:98322/1‑102 (MQ=255)                                                                         aaTGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATgcgcg                                                                                                                          <  2:98322/102‑1 (MQ=255)                                                                              gTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGaa                                                                     >  4:97404/1‑150 (MQ=255)                                                                              gTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGaa                                                                     <  1:120716/150‑1 (MQ=255)                                                                               tttATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACg                                                                                                           >  1:267558/1‑111 (MQ=255)                                                                               tttATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACg                                                                                                           <  2:267558/111‑1 (MQ=255)                                                                               tttATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACt                                                                               <  3:110532/139‑1 (MQ=255)                                                                               tttATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACt                                                                               >  4:110532/1‑139 (MQ=255)                                                                                          aaTATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacgctc                                                         <  4:529579/150‑1 (MQ=255)                                                                                             attatGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacgcccacg                                                      >  4:465408/1‑150 (MQ=255)                                                                                                 tGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCg                                                                                                  <  3:333670/102‑1 (MQ=255)                                                                                                 tGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCg                                                                                                  >  4:333670/1‑102 (MQ=255)                                                                                                 tGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCAcgcgcg                                                  >  1:564020/1‑150 (MQ=255)                                                                                                  gggAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACgcgcgc                                                 >  2:774996/1‑150 (MQ=255)                                                                                                      aaaGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTc                                             <  4:249427/150‑1 (MQ=255)                                                                                                      aaaGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAAGGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGTAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTc                                             <  2:507392/150‑1 (MQ=255)                                                                                                        aGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCg                                           >  2:755363/1‑150 (MQ=255)                                                                                                            gATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGa                                       <  3:83999/150‑1 (MQ=255)                                                                                                            gATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGa                                       <  4:138254/150‑1 (MQ=255)                                                                                                             aTGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAg                                      >  3:303532/1‑150 (MQ=255)                                                                                                                 aTTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTAt                                  >  2:882808/1‑150 (MQ=255)                                                                                                                  ttcgggAGAGGAAGAGGCTTCCACGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGGGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGGACAGTTCACGCTCCCGAGCGCCTTCCGCTGGGTCATc                                 >  2:417388/4‑150 (MQ=255)                                                                                                                     gggAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGt                              >  1:373732/1‑150 (MQ=255)                                                                                                                     gggAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGt                              <  1:794888/150‑1 (MQ=255)                                                                                                                      ggAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGCTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGtt                             <  4:468024/150‑1 (MQ=255)                                                                                                                         gagGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGcc                          <  4:170276/150‑1 (MQ=255)                                                                                                                          agGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCg                         <  1:253859/150‑1 (MQ=255)                                                                                                                            gAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtc                                                               <  1:838409/110‑1 (MQ=255)                                                                                                                            gAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtc                                                               >  2:838409/1‑110 (MQ=255)                                                                                                                            gAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCgag                       >  1:53444/1‑150 (MQ=255)                                                                                                                              agagTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAAGGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTCCCGCTGACCTATCGGTTGCCGacaa                     >  2:56442/1‑150 (MQ=255)                                                                                                                                agTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGagaaga                   <  1:156564/150‑1 (MQ=255)                                                                                                                                 cccttCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAAc                                                                                                            >  4:76503/4‑60 (MQ=255)                                                                                                                                 cccttCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAAc                                                                                                            <  3:76503/57‑1 (MQ=255)                                                                                                                                  tATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAAc                 >  4:578600/1‑150 (MQ=255)                                                                                                                                    ttCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCa               <  1:291573/150‑1 (MQ=255)                                                                                                                                       cccGGTCAGAC‑GACCGGGGCAGGGGAGAGATTAATGCGCGCGTCCTTGCTCAACGCCTAAGCCGGTCTGGGAGTGTATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGCGTTATCGGTTGCCGAGAAGAACCAGAt            >  2:714642/1‑150 (MQ=255)                                                                                                                                       cccGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACt                                                                               <  4:432257/83‑1 (MQ=255)                                                                                                                                       cccGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACt                                                                               >  3:432257/1‑83 (MQ=255)                                                                                                                                            tCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGa       <  2:321569/150‑1 (MQ=255)                                                                                                                                              aGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAgg     >  2:1299/1‑150 (MQ=255)                                                                                                                                               gac‑ggcCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGa    >  2:168550/1‑150 (MQ=255)                                                                                                                                               gac‑gacCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGc                                          >  2:234663/1‑112 (MQ=255)                                                                                                                                               gac‑gacCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGc                                          <  1:234663/112‑1 (MQ=255)                                                                                                                                               gac‑gacCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGa    >  2:377406/1‑150 (MQ=255)                                                                                                                                                 c‑gacCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAAt  <  2:107588/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                       AATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAC‑GACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAAT  >  NC_000913_3_KO_pgi/4281523‑4281816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.