New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	1343551 =	48 (0.990)	4 (0.090)	4/266	6.6	8.2%	intergenic (‑223/+46)	osmB/yciT	osmotically and stress inducible lipoprotein/global regulator of transcription; DeoR family
	?	NC_000913_3_pae_tpiA	1343581 =	46 (1.030)					intergenic (‑253/+16)	osmB/yciT	osmotically and stress inducible lipoprotein/global regulator of transcription; DeoR family
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAATTATTCCGTGAAATCTGCTCGCGCGCAGGGTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCATCAGATCGCTGGCGCGTATTTTCGAATCGGTAATCACGCGATTGAATCG  >  NC_000913_3_pae_tpiA/1343403‑1343692                                                                                                                                                      |                                                                                                                                              aaaTTATTCCGTGAAATCTGCTCGCGCGCAGGGTTGTATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGc                                                                                                                                               >  1:503094/1‑149 (MQ=255)       tgccGTGAAATCTGCTCGCGCGCAGGGTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGgatga                                                                                                                                         >  2:787759/3‑149 (MQ=255)          cGTGAAATCTGCTCGCGCGCAGGGTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGCTAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATATT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGTATGAttt                                                                                                                                      >  1:695985/1‑149 (MQ=255)           gTGAAATCTGCTCGCGCGCAGGGTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTg                                                                                                                                     >  2:751867/1‑149 (MQ=255)           gTGAAATCTGCTCGCGCGCAGGGTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTACATCTTGAGAGTGTTAATAACCGGTAAATAGC‑CTTAATTATCAACCAGGACTCCTCTTAGAGCGGATGAtttc                                                                                                                                     >  1:599745/1‑148 (MQ=255)            tGAAATCTGCTCGCGCGCAGGGTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGc                                                                                                                                    >  1:518620/1‑149 (MQ=255)                            tcAGCGTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAatcatc                                                                                                                    <  1:727256/148‑1 (MQ=255)                            gcAGGGTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGACAAACTGCAAatcatc                                                                                                                    >  1:116528/1‑149 (MQ=255)                                ggTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTa                                                                                                                >  1:537857/1‑149 (MQ=255)                                 gTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAAAAATTTTATATAATGACAGTGGTAATAACAGGTAAACATTGCATCA‑CATCACCGAGAAAGCACCTGAGAGCGGATGATTTGCCAAACTGCAAATCACCCGTag                                                                                                               >  2:605245/1‑149 (MQ=255)                                  ttATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTaga                                                                                                              >  1:789910/1‑149 (MQ=255)                                    atatCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCCGGACTCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTagaca                                                                                                            >  2:89460/1‑147 (MQ=255)                                     taacccGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTGTCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGaa                                                                                                           <  1:249024/144‑1 (MQ=255)                                      atCACGCCGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTCTATCTGGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAg                                                                                                          <  1:586163/149‑1 (MQ=255)                                       tCACGCAGTGGATAATTACTTCCTGTTATTTCGGTGATAATAATTTTATATCGTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAgg                                                                                                         <  1:774942/149‑1 (MQ=255)                                                     aTGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCCAATCATCCTAAGGAAAGGGAAATGGTTAAATg                                                                                           >  2:617275/1‑149 (MQ=255)                                                          ttgcTGTTATATCGCTGATAATAATTTTATATCTTAAGAGTGATAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAac                                                                                      <  1:267350/146‑1 (MQ=255)                                                               gTTATATCGCTGATACCTATTTGACGACATTAGAGGGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTATAGAAGGGAAATGGTTAAATGTCAACGACGt                                                                                 <  2:599745/149‑1 (MQ=255)                                                                     tCGGTGATAAGAATTTTACATCTTGAGAGTGTTAATAACTGGTAAATCGT‑CTTAATTATCAACCAGGAATCATCGTAGATCGGATGATTTGCCAAACTGCAAATCTTCCGTAGAGAAGGGAAACGGTTAAATGTCAACGACGTGAATgg                                                                           <  1:645467/149‑1 (MQ=255)                                                                      cGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGt                                                                          >  2:702098/1‑149 (MQ=255)                                                                       gCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTGGCCAAAGTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACCTGaccggta                                                                         >  2:645467/1‑143 (MQ=255)                                                                        cggATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATGCGTAGGGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGa                                                                        <  2:141442/147‑1 (MQ=255)                                                                        cTGATAATAATTTTATATCTTGTGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTATAGCGTATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGa                                                                        <  1:542490/149‑1 (MQ=255)                                                                         tgttaaaaaTTTTATATCTCGAGGGTGGTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCAGCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAg                                                                       <  2:308267/146‑1 (MQ=255)                                                                             aataatTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTa                                                                   <  2:576769/149‑1 (MQ=255)                                                                                         tCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGa                                                       <  1:49806/149‑1 (MQ=255)                                                                                           ttGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGAGGATTTGCCAAACTGCAGATCATCCGTAGAGCAGGGCAATGGTTAAATGTCAACGACGTGAATGGTGCGTTTCCTGTGCTCAAGacg                                                     >  1:61671/1‑147 (MQ=255)                                                                                           ttGAGAGTGTTAATAACAGATAAATAGT‑CTTAATTATAAACAAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAATAGTAAACGACGTGAATGGTGGGTTTACTGTGCTCAAGATg                                                     >  2:695247/1‑149 (MQ=255)                                                                                            tGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGc                                                    >  1:518280/1‑149 (MQ=255)                                                                                            tGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGc                                                    >  2:205834/1‑149 (MQ=255)                                                                                              agagTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCAt                                                  <  2:537857/149‑1 (MQ=255)                                                                                                agTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCATCa                                                >  1:569823/1‑149 (MQ=255)                                                                                                          aCAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGATAAGTTTACTGTGCTCAAGATGCATCAGACAGCTGgc                                      >  2:365468/1‑149 (MQ=255)                                                                                                                      t‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGTAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCCAGATGCATCAGATCGCTGGCGCGAATTTTCGa                          >  2:351868/1‑149 (MQ=255)                                                                                                                        cTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCATCAGATCGCTGGCGCGTATTTTCGaa                         >  2:777580/1‑149 (MQ=255)                                                                                                                             ttATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCATCAGATCGCTGGCGCGTATTTTCGAATCGGt                    <  1:125022/149‑1 (MQ=255)                                                                                                                               aTCAACTAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCATCAGATCGCTGGCGCGTATTTTCGAATCGGTaa                  <  2:61671/149‑1 (MQ=255)                                                                                                                               aTCAACCAGGAATCATCTTAGAGCCGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATCGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCATCAGATCGCTGGCGCGTATTTTCGAATCGGTaa                  <  1:215003/149‑1 (MQ=255)                                                                                                                                  aaCCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGTAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCATCAGATCGCTGGCGCGTATTTTCGAATCGGTAATCa               >  1:305091/1‑149 (MQ=255)                                                                                                                                    ccAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCATCAGATCGCTGGCGCGTATTTTCGAATCGGTAATCAcg             >  2:62091/1‑149 (MQ=255)                                                                                                                                     cAGGAATCATCTTAGAGGGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCATCAGATCGCTGGCGCGTATTTTCGAATCGGTAATCAcgc            <  2:298502/149‑1 (MQ=255)                                                                                                                                      aGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCATCAGATGGCTGGCGAGTATTTTCAAGTAGGTAAGCAcgct           >  2:121208/1‑148 (MQ=255)                                                                                                                                        gAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCAt                                                  >  2:676948/1‑108 (MQ=255)                                                                                                                                        gAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCAt                                                  <  1:676948/108‑1 (MQ=255)                                                                                                                                            catcTTAGAGCGGAATATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCATCAGATCGCTGGCGCGTTTTTTCGAATCGGTAATCACGCGATTGaa     <  1:168237/149‑1 (MQ=255)                                                                                                                                              tcTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATTGTGAGTTTACTGTGCTCAAGATGCATCAGATCGCTGGCGCGTATTTTCGAATCGGTAATCACGCGATTGAATc   <  2:518280/149‑1 (MQ=255)                                                                                                                                              tcTTAGAGCGGATGATTTGCCAAACTGCAAAGCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCATCAGATCGCTGGCGCGTATTTTCGAATCGGTAATCACGCGATTGAATc   <  2:232688/149‑1 (MQ=255)                                                                                                                                               cTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCATCAGATCGCTGGCGCGTATTTTCGATGCGGTAATCACGCGATTGAGTCg  >  1:366858/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                              AAATTATTCCGTGAAATCTGCTCGCGCGCAGGGTTATATCACGCAGGTGATAATGACTTCCTGTTATATCGCTGATAATAATTTTATATCTTGAGAGTGTTAATAACAGGTAAATAGT‑CTTAATTATCAACCAGGAATCATCTTAGAGCGGATGATTTGCCAAACTGCAAATCATCCGTAGAGAAGGGAAATGGTTAAATGTCAACGACGTGAATGGTGAGTTTACTGTGCTCAAGATGCATCAGATCGCTGGCGCGTATTTTCGAATCGGTAATCACGCGATTGAATCG  >  NC_000913_3_pae_tpiA/1343403‑1343692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.