New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	3383284 =	42 (1.440)	4 (0.150)	4/258	3.4	9.5%	intergenic (+17/+46)	degS/mdh	serine endoprotease, periplasmic/malate dehydrogenase, NAD(P)‑binding
	?	NC_000913_3_pae_tpiA	3383314 =	38 (1.420)					intergenic (+47/+16)	degS/mdh	serine endoprotease, periplasmic/malate dehydrogenase, NAD(P)‑binding
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATA  >  NC_000913_3_pae_tpiA/3383139‑3383429                                                                                                                                                   |                                                                                                                                                  tctgctctgGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGACTATCCGGCAACCAATTAAGTCGTGCGCTcaaaac‑aaaa                                                                                                                                                >  1:11942/1‑149 (MQ=255)        tgGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACGATTCAGGAATATCCGGCAACCAATTAAGGCGTGCGCTCAAAAC‑AAAAAAACGGa                                                                                                                                         >  1:369075/1‑149 (MQ=255)         ggAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAg                                                                                                                                        <  1:411164/149‑1 (MQ=255)               gATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAGCCAATTAAGTCGTGCCGTCAAAAC‑AAAAAACCGGAGTCtgtg                                                                                                                                  >  2:85306/1‑149 (MQ=255)               gATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGACGTGCGCTCAAAAC‑AAAAAACCGGAGTCtgtg                                                                                                                                  >  2:126272/1‑149 (MQ=255)                      cAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGt                                                                                                                           <  1:59399/149‑1 (MQ=255)                            gCGGAAATTCGCCCTGGTGCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTtta                                                                                                                     <  1:211295/149‑1 (MQ=255)                              ggAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCACAAC‑AAAAAACCGGAGTCTGTTCTCCGGTGTTttatt                                                                                                                   >  1:469266/1‑149 (MQ=255)                               gAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTttatta                                                                                                                  <  1:7267/149‑1 (MQ=255)                                               cGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCGAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTAc                                                                                                  <  2:513498/149‑1 (MQ=255)                                                 gTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACAGGAGTGTGTGCTCCGGGTTTGTATTATCGGCTAATCAATTACtt                                                                                                >  1:138682/1‑149 (MQ=255)                                                       ccTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGATGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAAc                                                                                          <  1:272445/149‑1 (MQ=255)                                                       ccTGTAGTAGTGATGCGTGATGATAAGCAGTTAACCACCCAGGACACCACTCAGGACTCCACAGCAATAAAAAAAAAAGTGCGATCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTCGTATATTATCCGCTAATCAATTACTTATTAAc                                                                                          >  2:252590/1‑149 (MQ=255)                                                         tGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCCATTAAGTCGTGCGCTCAAAAC‑AAACAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTGATCAATTACTTATTAACGa                                                                                        >  1:59912/1‑149 (MQ=255)                                                         tGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGa                                                                                        <  2:401020/149‑1 (MQ=255)                                                            agtagtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAAct                                                                                     <  1:111530/149‑1 (MQ=255)                                                                       gTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCacg                                                                          >  1:78154/1‑147 (MQ=255)                                                                        tgatgaTAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAggg                                                                         <  1:106929/149‑1 (MQ=255)                                                                                  cAGTTAACGCTGCAGGTGACCATTCAGCAATATCCGGCAACCAGTTAAGTCGTGCGATCAAAAC‑AAAAACCCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTGATTAACGACCTCTTCGCCCCGGGCGATCtcttt                                                               <  2:351995/149‑1 (MQ=255)                                                                                        aCGGTGCAGGTCACTATTGAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAg                                                         <  2:369075/149‑1 (MQ=255)                                                                                          gCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTCATAAATCACGAATAAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCg                                                       >  1:40182/1‑149 (MQ=255)                                                                                            tGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTaa                                                                                           <  1:93696/111‑1 (MQ=255)                                                                                            tGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTaa                                                                                           >  2:93696/1‑111 (MQ=255)                                                                                             gaaGGTCACCATTCAGGAATATCCGGGAACCAATTAAGTCGTGCGCTCAAAACAAAAAAACCGGAGTCTGTGCGCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTa                                                     <  2:77707/147‑1 (MQ=255)                                                                                                tgTCACCATTCAGGAATATCCTGCAACAAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCa                                                 <  1:55415/148‑1 (MQ=255)                                                                                                 gTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAg                                                >  1:454876/1‑149 (MQ=255)                                                                                                 gTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAg                                                >  2:179698/1‑149 (MQ=255)                                                                                                 gTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAg                                                >  1:321698/1‑149 (MQ=255)                                                                                                   aaCCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCa                                              <  1:85306/148‑1 (MQ=255)                                                                                                    aCCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATc                                                                                                                <  2:268638/82‑1 (MQ=255)                                                                                                    aCCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATc                                                                                                                >  1:268638/1‑82 (MQ=255)                                                                                                          cAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAAc                                                                                          >  2:60820/1‑98 (MQ=255)                                                                                                          cAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAAc                                                                                          <  1:60820/98‑1 (MQ=255)                                                                                                           aGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCCGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTcc                                      >  2:290430/1‑149 (MQ=255)                                                                                                              aaTATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAgc                                   <  1:437519/149‑1 (MQ=255)                                                                                                                         aCCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGCGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTc                        >  2:450871/1‑149 (MQ=255)                                                                                                                            aaTTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCaaa                     >  2:244908/1‑149 (MQ=255)                                                                                                                             aTTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAAt                    <  1:390254/149‑1 (MQ=255)                                                                                                                             aTTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAAt                    <  1:450871/149‑1 (MQ=255)                                                                                                                                      cgggcTCAAACG‑AAATAACCGCCTTCTGTGCTCCGGTTTTTTATTACCCGCTAATCAATTACTTACTAACGAACTCGTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAgg           <  2:404306/146‑1 (MQ=255)                                                                                                                                           tCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTAATTATTAACGAACTCTTCGCCCAGGGCGATATCTTTATTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTTcc      >  1:228176/1‑149 (MQ=255)                                                                                                                                               aac‑aaaaAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATa  <  1:179698/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  TCTGCTCTGGAGACGATGGATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAAC‑AAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATA  >  NC_000913_3_pae_tpiA/3383139‑3383429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.