Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F9 I0 R1	131	136.1	2723430	98.1%	2671684	229.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,571,181	A→C	18.1%	V132G (GTG→GGG)	yjiR ←	putative DNA‑binding transcriptional regulator/putative aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,571,181	0	A	C	18.1%	183.2 / 5.0	94	V132G (GTG→GGG)	yjiR	putative DNA‑binding transcriptional regulator/putative aminotransferase
Reads supporting (aligned to +/- strand): ref base A (40/32); new base C (8/13); total (48/46)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGCATCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGG‑TTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCACCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCAT  >  NC_000913/4570948‑4571419

gcagcaTCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGG‑TTTACACCCTGCCATTACCGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCCCCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCaccacc                                                                                                                                                                                                                                               <  2:876405/239‑1 (MQ=255)

gcagcaTCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGG‑TTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCACCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCaccacc                                                                                                                                                                                                                                               <  1:1014690/239‑1 (MQ=255)

         gCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGG‑TTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGGCCACCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCCGACCGGC‑TAACAAGTC‑ATAACCGAGAAAGGTTTTCAGATTATGCTGCACCACCCGGCTTa                                                                                                                                                                                                                                        >  1:232097/1‑237 (MQ=255)

                    taataaCAGGGGGATTCGACCGCGACAATATCGCCCGG‑TTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGGGACCACCGAGGCGCCGTC‑GAGGAACAGGCGGGGCAACCGCTCTCGCGATACCCGCTGGCCCGC‑CAACCAAGC‑CTAACCCAGAAAGGGGTGCCGATTATTCTGCACCCCCCCGcctctccccccccccc                                                                                                                                                                                                                            >  2:260298/1‑223 (MQ=255)

                    taataaCAGGGCGGTTCGCCGCCAAAAATCCCGCCCGG‑TTTACACACGGCCATTAACCCCGGCGACATCGAGTTAGGGAAGCCGTGGTTGATGAGGCTGC‑CATCGGCGGTGACCCCCGAGCGGCTGTC‑GAGCATCAGGCGGGCAAGCTGCTCTCGCAATCCTCGCTGACCGGC‑TAACAAGTC‑AAAACCGAGAACGGTTTGCAGATTATGCTGCCCCACCCGGCTTAGCTCCCGCCA‑CAg                                                                                                                                                                                                                          <  2:667114/239‑1 (MQ=255)

                    aaaaaaCAGGGGTATTCGCCCGCGAAAAT‑TCGCCCGGTTTTACCCCCTGCCATTACCGCCAGGGACATCGAGTTAGGGCCGCCGCGGGTGATGAGGATGT‑CATGGGGGGTGACCCCCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGTTTAGCTCCCGCCA‑CAg                                                                                                                                                                                                                          <  2:399418/235‑1 (MQ=255)

                     aatagaggggtgATGCGCCCGCGACAATATGGCCCGT‑TTCACGCGCTGCCATTACCGCCCGCGACCTCGATTTATGGCAGCCGCTGGGGATGATGATGT‑CATCGCGGGTGACCCCCGGGCCGCTGTC‑GGGCATCAGGCGGGCAATCTGCTCTCGCAATCCTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGTTTAGCTCCCGCCA‑CAg                                                                                                                                                                                                                          <  2:854414/231‑1 (MQ=255)

                            gggggATTCGACCGCGACAATATCGCCCGG‑TTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCCCCGGGCCGCTGTC‑GGGGATCCGGCGGGCAATCTGCTCTCCCCATACTCCCCGCCCGGC‑TAACAAGGC‑ATAACCCGGAACGGGTTGCAGAGTATTCTGGCCCACCCGGCCTAGCTcccccccccgagggttta                                                                                                                                                                                                                   >  2:209474/1‑227 (MQ=255)

                              gggATTCGACCGCGACAATATCGCCCGG‑TTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGGCCCCCCCGCCCCTGTCGGGGAACCCGGCGGGCAAAC‑GCCCTCCCCACACCCCCCGACCCGC‑TCACAAAGC‑ATAACCGACAACGGGTTTCCGGTTATGCTGCCCCACCCCGCCTCGCTCCCCCCC‑CAGCCG‑CTTccg                                                                                                                                                                                                                >  2:695187/1‑236 (MQ=255)

                              gggATTCGACCGCGACAATATCGCCCGG‑TTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCACCGAGCCCCCGTC‑GAGGATCAGGGGGGGAATCTGCTCTCCCAATACTCGCTTAACGGC‑CAAAAAAAC‑ATAAAACAAGACGGGTTGGCGATTATTCTTCACCCCCCCGCTTATCCCCCCCCC‑CAGCGG‑tgtgcg                                                                                                                                                                                                                >  2:528470/1‑233 (MQ=255)

                                    cgcccGCGACAATACCGCCGGG‑TTTACCCCCTGCCATTAACGCCAGCAACACCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCCCCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGgc                                                                                                                                                                                                         <  1:107049/236‑1 (MQ=255)

                                     gACCGCGACAATATCGCCCGG‑TTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCCCCCAGCCGCCGTC‑GGGCCTCAGGCGGGCCATCTGCTCTCGCCATACTCGCTGGCCGGC‑CAACAAGTC‑ATAACCGAGAACGGGTTTCAGATTATTCTGCCCCCCCCGCCCTAGCTCCCGCCC‑CAGCGG‑TTTCAGGCTggggg                                                                                                                                                                                                        >  1:881069/1‑237 (MQ=255)

                                     gACCGCGACAATATCGCCCGG‑TTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCACCCAGCCGCCGTC‑GAGCACCAGGGGGGCGATCTGCTCTCGCAACACTCCCTGCCCCGC‑CAACAAAGC‑CTAAACCCGAACGGGTTGCAGATTATGTTGTCCCACCCGCCCTAGCTCCCGCCA‑CACCGG‑TGTTAGGCTgggg                                                                                                                                                                                                         >  2:1057589/1‑237 (MQ=255)

                                       ccGCGACAATATCGCCCGG‑TTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGTCCACCGGCCCGCCGTC‑GCGCATTCGGCGGGCGATCTGCTCTCGCCATACTCGCCGACCCGG‑CCACAAATC‑AATACCCACGACGGGTTGCCGATTTTGCCGCACCACCCCGCCTAGCGCCCCCCC‑CACCGG‑GTTccgctctggcggc                                                                                                                                                                                                      >  2:160782/1‑227 (MQ=255)

                                       ccGCGACAATACCGCCCGT‑TTTACCCACTCCCATTAACGCCAGCGACATCGAGTTAGGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCACCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCt                                                                                                                                                                                                      <  1:1208278/239‑1 (MQ=255)

                                          cgACAATATCGCCCGG‑TTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCCCCCGGCCGCCGTT‑GAGCATCAGGCGGGCAAACTGCTCCCGCCATAACCCCTGACCGGG‑CAACAAGAC‑ATAACCGAGAAAGGGTTTCAGATTATGCTGCCCCCCCCCGCGTAACCCCCCCCC‑CAACGG‑GTTCGCGCCGGGCGCTTc                                                                                                                                                                                                    >  2:650000/1‑238 (MQ=255)

                                                             tACACACTGCCATTACCGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCACCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTg                                                                                                                                                                                <  1:702148/239‑1 (MQ=255)

                                                               cacacTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCACCCAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAAAAAGTC‑ATAAACGAGAACGGTTTGCAGATTATTCTTCACCCCCCCGCTTAGCTCCCCCCC‑CACCGG‑TTTTAGGCTGGGCGCCTCC‑ACCTCCCGTGTGCTTTTGCt                                                                                                                                                                              >  1:912251/1‑239 (MQ=255)

                                                                   cTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGGGACCACCCAGCCCCCTTC‑GCGCATCAGGCGGGCAAACTGCCCCCGCCAAACTCGCCGGCCGGC‑CAACAAATC‑ATAAACGGGAACGGGTTTCAAGTTCTTCTTCACCACCCCGCCTTACTCCCCCCA‑CCGCCG‑CTTCAGGCTGTGCGCGTCC‑ACAAGCAGTGGGCTTTTGCttta                                                                                                                                                                           >  2:758712/1‑236 (MQ=255)

                                                                    tGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCACCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAAAGGGTTGCAGATTATTCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑GTTCAGGCTGGGCGCTTCG‑ACATACGGTGTGCTTTTGCTTAACg                                                                                                                                                                         >  1:284359/1‑239 (MQ=255)

                                                                    tGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCAACCCGACGCTGTC‑CCGGATCCGGGGGGGAACATTCCCTCCCCATACTCGCCCAGCCGC‑TCAACAGAC‑ATAACCGAAAAAGGTTTGGCGCGTATGCTTCACCACCCCGCTTCGCTCCCCCCC‑CACCCC‑GTTTAGGCTTGGCGCGTCG‑CCATCCGCTGTGGttttctttttac                                                                                                                                                                         >  2:847283/1‑231 (MQ=255)

                                                                     gCCATTACCCCCAGCGACTTCGAGTTTTGGCAGCCGCTGGGAATGATGATGT‑CATCGGCGGGGACCCCCGAGCCGCTGTC‑GGGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACgg                                                                                                                                                                        <  1:334330/239‑1 (MQ=255)

                                                                     gCCATTAACGCCAGCGACATCGATTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCACCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACgg                                                                                                                                                                        <  1:839471/239‑1 (MQ=255)

                                                                     gCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGGGACCACCCAGCCCCCGGC‑GGGCATCCAGCGGGCAATCTTCTCTCCCCATACTCGCTGACCCGC‑TCACAAATC‑CTAACCCAGAACGGGTTGCAGACTTTTCTTCCCCACCCCGCCTTGCCCCCCCCC‑CCCCGG‑GTTCAGGCTGGGCGCCTCC‑GCCACCCGTGGTCTTTTTCTTaaacg                                                                                                                                                                        >  2:702148/1‑236 (MQ=255)

                                                                     gCCATTAACGCCAGCGACATCGAGTTATGGCAGCAGCTGGTGATGATGATGT‑CATCGGCGGTGACCACAAGGCCGCTGTC‑GCGCAATAGGCGGGGAATCTACTCTCGCCACACTCGCTGCACCGC‑CAGCAAGAC‑ATAACCGCAAACAGGTTGGAGATTATGGCGCACCACCCGGCGTAGCTCCCGCCC‑CAGCGGCGTTCAGAGT‑GGCGCGTCG‑CCATCCGGTGGTCGTTTGCttcaagg                                                                                                                                                                        >  2:778092/1‑234 (MQ=255)

                                                                       cATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCCCCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGaa                                                                                                                                                                      <  1:528470/239‑1 (MQ=255)

                                                                             cGCCAGCGACATCGAGTTAGGGCGGCCGCTGGTGATGATGATGT‑CACCGGCGGTGACCACCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATgg                                                                                                                                                               <  1:664138/239‑1 (MQ=255)

                                                                               ccAGCGCCATTGGGTTTTGGCAGCCCCTGTTGTTGATGTTGC‑AACCGGAGTCGACCTCCGGGCAGCTGCA‑GGGCATCAGGCGGGCAATGTGCTCTCGAAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGACCGGTTTGCAGATTATGCTGCACCCCCCGGGTTAGCCCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGaa                                                                                                                                                             <  2:1056881/239‑1 (MQ=255)

                                                                                cAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGGCCACCGCGCCGCCGTC‑GGGCAACCGGGGGGCCATCCGCTCTCGCCATACTCGCCGACCCGC‑CCACAAGTC‑ATAACCGCGAACGGGTTGCCGATTATTCTTCACCACCCCGCCTTGCTCCCCCCC‑CCGCGG‑GTTCAGGGCGGGCGCTTTG‑GCAACCGCGGTGCTTTTTTTTTACGGGAC‑aagtgga                                                                                                                                                             >  2:808669/1‑233 (MQ=255)

                                                                                 aGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCACCCAGCCGCCGTC‑GGGCATCAAGCGGGGCATCTGCTCTCGCCATACTCCCCGACCGGC‑TCACAAATC‑ATAACCCAGAACCGTTTTCCGATTTTGCTGCCCCCCCCCGCCTTACTCCCCCCA‑CCGCGG‑GTTCCGGCTGGGCGCTTCG‑CCCTTCCGGGTGCTTTTTCTTTACGGAAC‑AATgggaaa                                                                                                                                                           >  2:164456/1‑235 (MQ=255)

                                                                                  gCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGAACACCCAGCCGCTGTC‑GGGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑CAACAAGTC‑ATAACCCAAAACGGGTTGCCGATTATTCTGCACCACCCGGCCTAGCTCCCGCCC‑CCGCGG‑GTTCATGCTGGGTGCTTCC‑ACAACCGGTGTGCTTTTTCTTacatgaaaaattgaaatg                                                                                                                                                           >  2:1104804/1‑222 (MQ=255)

                                                                                    gccATCGAGTATGGGCGGCCGCGGGGGATGATGAGGC‑ACTCGGCGGTGACCACCGAGCCGCTGTC‑GGGCATCAGGCGGGCAATCTGCTCTCGCAATACGCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCCCCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGt                                                                                                                                                         <  2:522531/236‑1 (MQ=255)

                                                                                    gACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCACCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGt                                                                                                                                                         <  1:862996/238‑1 (MQ=255)

                                                                                             ttATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGAACACCCAGGCGCTGTC‑GAGCCTCCGGCGGGCAATCTGCTCTCCCAATAATTGCTGACCGGC‑TAACAAATC‑ACAAACGAGAAAGGGTTGCCGATTATGCCGCACCCCCCCGCCTATCTCCCCCCA‑CCGCGG‑GTTCAGGCTTGGCGCCTCC‑GCACCCGGCGTTCCTTTTCTTTACGGCAC‑AATGGAAcgttttctctgcc                                                                                                                                                >  2:704721/1‑226 (MQ=255)

                                                                                               gtggcgcccGCGGGTGATGTGTAGGT‑CATGTGGGTTGCCCCCCGGGCCGCTTTC‑GAGCATCAGGCGGGCATTCTGCTCGCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCCCCCCCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCt                                                                                                                                             <  2:1068534/232‑1 (MQ=255)

                                                                                               aTGGCCGCCGCTGGGGATGATGATCT‑CATCGGCCGTGCCCGCCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCt                                                                                                                                             <  1:759552/239‑1 (MQ=255)

                                                                                               aTGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCACCGAGCCGCCGTC‑GGGCGTCAGGCGGGGAATCTGCTCTCGCCATACTCGCTGACCCGC‑CAACAAAAC‑ATAAACGAGAACGGGTTTGAGATTATGCTGCCCCACCCGGCCTAGCCCCCGCCC‑CCGCCG‑GTTCCGGCTGGGCGGTTTG‑ACATCCCGGGTGCTTTTTTTTTACGGGACAAATGGAA‑TGTCGCCATGCGcc                                                                                                                                              >  2:69540/1‑238 (MQ=255)

                                                                                                tggcggcgagtGGTGAGGTGGATGT‑CATCGCGGGTGCCCACCGAGCCGCGGTC‑GAGCAACAGGCGGGAAATCTGCCCCCGCAATACTCGCGGACCGGC‑TAACAAGTC‑ATACCCGAGACCGTTTTGCAGATTATGCTGCACCACCCGGCTTAGCCCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCt                                                                                                                                             <  2:358194/228‑1 (MQ=255)

                                                                                                     gCCGCTGGTGGTGATAATGG‑CAGCGGCGGTGACCCCCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCCCCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGc                                                                                                                                        <  1:351191/238‑1 (MQ=255)

                                                                                                     gCCGCTGGTGATGATGATGT‑CATCGGCGGGGACCACCCCGCCGCTGTC‑GGGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTTACCGGC‑TAACAAAGC‑ATAAACGCGGACCGGTTGCAGATTATGCTGCACCCCCCCGCTTTGCTCCCGCCC‑CCGCGG‑GTTTAGGGTGGGCGCGTCG‑GCACCCCGGGGGCTTTTTGTTAACGGGAC‑AATGGGACTGTCGcccttcggccccacca                                                                                                                                       >  2:823188/1‑224 (MQ=255)

                                                                                                      gagaggtgtataagagacggcATCGGCGGTGACCCCCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGc                                                                                                                                        <  1:819836/218‑1 (MQ=255)

                                                                                                      ccGCTGGTGATGATGATGT‑CATCGGCGGGAACCACCGAGCCGCTGTC‑TAGCATCAAGGGGGCAATCTGCTCTCCCAAAAATCGCGTACCGGC‑TAACAAGTC‑ATAACCGCGAAAGGGTTTAAGATTATGCTGCACCACCCCGCTTTGCTCCCGCCC‑CAACGG‑GTTTAGGCGGGGAGCTTCGAACCTCCGG‑GTGCTTTTGTCTTACGCGAC‑AATGGAAccttgcggatgcgcccccaggct                                                                                                                                      >  2:578871/1‑217 (MQ=255)

                                                                                                       ggtggggggagatgatgT‑CACGGGCGGTAACCCCGGAGCCGCTGGC‑GAGCATCGGGCGGGCAATCTGCTCTCGCAATACGCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCCCCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCata                                                                                                                                     <  2:994885/229‑1 (MQ=255)

                                                                                                       cGCTGGTGATGATGATGT‑CATCGGCGGTGACCACCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCCCCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGc                                                                                                                                        <  1:1057589/236‑1 (MQ=255)

                                                                                                       cGCTGGGGAGGATGATGT‑CATCGGCGGTGCCCACCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCCCCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGc                                                                                                                                        <  1:197539/236‑1 (MQ=255)

                                                                                                          tGGTGATGATGATGT‑CATCGGCGGTGACCACCGAGCCGCTGTC‑GAGCATCAAGCGGGCAATCTGCTCTCGCCATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCCAGAACGGGTTGCAGATTATGCTGCACCACCCCGCTTTGCTCCCCCCACCACCGG‑TTTC‑GGCTGGGGGCCTCG‑ACATCCCGTGTGCTTTTTGTTTACGGAAA‑AAATGAACTTTCGCCATGTGCCTcccccctatc                                                                                                                                   >  2:1057597/1‑230 (MQ=255)

                                                                                                             tgatgatgaTGT‑CATCGGCGGTACCCACCAAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCCCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGACCGGTTTGCAGATTATGCTGCCCCACCCGGCTTAGCCCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAg                                                                                                                                <  1:778092/238‑1 (MQ=255)

                                                                                                             tgatgatgaTGT‑CAGCGGCGGTGCCCACCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCCCCCCCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGTATATCCAg                                                                                                                                <  1:1104804/238‑1 (MQ=255)

                                                                                                             tgaggatgaTGT‑CATCGGCGGTGACCACCGGGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATACCCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAg                                                                                                                                <  1:145293/238‑1 (MQ=255)

                                                                                                              gatggtgatGTCCATGGGCGTGGCCCCCCGGGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAg                                                                                                                                <  2:1203986/238‑1 (MQ=255)

                                                                                                               atgatgatgT‑CATCGGCGGTGACCACCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCa                                                                                                                              <  1:991999/238‑1 (MQ=255)

                                                                                                                      tgT‑CACCGGCGGTGCCCACGGAGCCGCTGTC‑GGGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATcc                                                                                                                      <  1:695187/239‑1 (MQ=255)

                                                                                                                       gT‑CATCGGCGGTGACCACCCCGCCGCCGGC‑GTGCATTACGCGGGCAAACTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGGC‑CTAACCGAGAACGGCTTGCAGATTATGCTGCACCACCCGGCCCAGCCCCCCCCC‑CAGCGG‑TCTCAGGCCGGGCGCTTCT‑GCCTCCCGGGTGCTTTTTCCTCACAGAAC‑AATGGACATGTCGCCATTCCCCCTCCCCATATCCAGCAccctctcc                                                                                                                      >  2:440154/1‑232 (MQ=255)

                                                                                                                           atgggcggTGCCCCCCGAGCCGCTTGC‑GACCATCAGGGGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACt                                                                                                                  <  1:218185/236‑1 (MQ=255)

                                                                                                                                     accaccGAGCCGCTGTC‑GGGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAAAAAATC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCCGCTTAGCTCCCCCCC‑CAGCGG‑TTTCAGGCTGGGCGCTTCC‑ACCTCCGGGGTGCTTTTGCTTAACGGGAC‑AATGGAACTGTTGCCATGCGGCTCCAGCATATCCAGCa                                                                                                                              >  2:819836/1‑217 (MQ=255)

                                                                                                                                             gCCGCGGCC‑GAGCCTCAGGCGGCCAATCTGCTCTCGCAATACGCGC‑GACCCGCATAACAATTC‑ATAACCGAGAGCGGTTTGCAGATTAGGCTGCACCCCCCGGCTTCGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCggg                                                                                                 <  2:791878/238‑1 (MQ=255)

                                                                                                                                                cgcgggcaggCAACAGGCGGGCAATCTGCTCTCGAAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCCCCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGgcg                                                                                               <  2:458069/229‑1 (MQ=255)

                                                                                                                                                 cTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCt                                                                                             >  1:806623/1‑238 (MQ=255)

                                                                                                                                                 cTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCCCt                                                                                             >  2:303397/1‑238 (MQ=255)

                                                                                                                                                  tGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTg                                                                                            >  1:209360/1‑238 (MQ=255)

                                                                                                                                                           aTCAGGCGGGCAATCGGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGa                                                                                     <  1:1326324/237‑1 (MQ=255)

                                                                                                                                                             cAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCa                                                                                          <  1:466326/230‑1 (MQ=255)

                                                                                                                                                             cAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCa                                                                                          >  2:466326/1‑230 (MQ=255)

                                                                                                                                                             cAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGAcgcg                                                                                 >  1:828875/1‑239 (MQ=255)

                                                                                                                                                               ggCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGt                                                                                >  2:957053/1‑238 (MQ=255)

                                                                                                                                                               ggCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTc                                                                               >  1:531807/1‑239 (MQ=255)

                                                                                                                                                               ggCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑T‑ACAAGGCAATACCCGAGAACGGTTGGCGAATTATCCTCAACCACCCGCCTAACCCCCCCCCC‑CAGGGG‑TTTAGGGCTGGCCCCTCCG‑ACTCCCGGGGGCCTTTTCCTTACGGGAAC‑ATGGCAACGGCCG‑TACGCGCCCCCAGCCAATCCCACCCCCGATTCCACCGGGGAATTTCCCCCCGgccgctcaccagaccccgc                                                                               >  2:218404/1‑221 (MQ=255)

                                                                                                                                                                 cGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTc                                                                               <  1:704392/237‑1 (MQ=255)

                                                                                                                                                                   ggCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATcg                                                                           >  1:678937/1‑239 (MQ=255)

                                                                                                                                                                        tCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGt                                                                      >  1:1019471/1‑239 (MQ=255)

                                                                                                                                                                              ctcGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTAccaga                                                                >  1:71717/1‑235 (MQ=255)

                                                                                                                                                                                cGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGGAGTggcg                                                               >  2:498500/1‑238 (MQ=255)

                                                                                                                                                                                  cAATCCTCGCTGACCGGC‑TAACAAGTC‑ATACCCGAGAACGGTTTGCAGATTATGCTGCACCCCCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCt                                                            <  1:94576/239‑1 (MQ=255)

                                                                                                                                                                                  cAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGGACTggcggcg                                                            >  1:340034/1‑238 (MQ=255)

                                                                                                                                                                                   aaTACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCt                                                            <  1:650000/238‑1 (MQ=255)

                                                                                                                                                                                      aCTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGGACtcgagggcctgg                                                        >  2:498261/1‑228 (MQ=255)

                                                                                                                                                                                         cGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACCggggggggggggtt                                                     >  2:795891/1‑230 (MQ=255)

                                                                                                                                                                                          gCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGCGCTGGGGGGTgggggttt                                                    >  2:1312318/1‑234 (MQ=255)

                                                                                                                                                                                           cTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCCGGGGttt                                                     >  1:1221390/1‑237 (MQ=255)

                                                                                                                                                                                             gACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGGACTGGCGGCTgggggttt                                                    >  2:199655/1‑231 (MQ=255)

                                                                                                                                                                                               ccGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTAcctggggcgcggcgtttactgt                                               >  2:1071990/1‑218 (MQ=255)

                                                                                                                                                                                                     tAACAAGTC‑ATACCCGAGAACGGTTTGCAGATTATGCGGCCCCCCCCGCCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGa                                          <  2:1289995/239‑1 (MQ=255)

                                                                                                                                                                                                       aCAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCCGGGGCTTTCGTTGTGGGaga                                        >  1:750564/1‑237 (MQ=255)

                                                                                                                                                                                                                   ccGAGAACGGTTTGCAGATTATGCTGCCCCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTAGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTtg                                               <  1:551282/221‑1 (MQ=255)

                                                                                                                                                                                                                    cGAGAACGGTTTGCAGATTATGCTGCCCCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGaa                            <  1:1201727/239‑1 (MQ=255)

                                                                                                                                                                                                                    cGAGAACGGTTGGCAGATTATGCTGCACCACCCGGTTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGaa                            <  1:358704/239‑1 (MQ=255)

                                                                                                                                                                                                                      agaACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGGTGGGGTTTTCGTTTTGGGACAAAAAAACCCgcaa                           >  1:962368/1‑235 (MQ=255)

                                                                                                                                                                                                                       gaACGGTTTGCAGATTATGCTGCCCCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAAcg                          <  1:1057597/238‑1 (MQ=255)

                                                                                                                                                                                                                         aCGTTTTGCAGATTATGTTGCACCACCGTCCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCgg                       <  1:402910/239‑1 (MQ=255)

                                                                                                                                                                                                                             tttGCAGATTATGCTGCCCCCCCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTg                    <  1:355853/238‑1 (MQ=255)

                                                                                                                                                                                                                             tttGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTAGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGGGGGTGGGGCTTTCGTTTTTCGGCaaaaactgtttctttttaacaa                   >  2:551282/1‑221 (MQ=255)

                                                                                                                                                                                                                              ttGCAGATTATGCGGCCCCCCCCGGTTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCg                  <  2:159589/239‑1 (MQ=255)

                                                                                                                                                                                                                                    aTTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCa                                                                                                                                       <  1:665011/116‑1 (MQ=255)

                                                                                                                                                                                                                                    aTTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCa                                                                                                                                       >  2:665011/1‑116 (MQ=255)

                                                                                                                                                                                                                                     ttAGGCTGCGCCCCCCGGATTAGCCCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGtgat           <  2:678937/239‑1 (MQ=255)

                                                                                                                                                                                                                                           tgcACCGCCCGGCTTAGCTGCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCt      <  1:1211854/238‑1 (MQ=255)

                                                                                                                                                                                                                                              accaccCGCCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCAt  <  1:782201/239‑1 (MQ=255)

GCAGCATCTGCATCGAACCGTAATAACAGGGGGATTCGACCGCGACAATATCGCCCGG‑TTTACACACTGCCATTAACGCCAGCGACATCGAGTTATGGCAGCCGCTGGTGATGATGATGT‑CATCGGCGGTGACCACCGAGCCGCTGTC‑GAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGC‑TAACAAGTC‑ATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCA‑CAGCGG‑TTTCAGGCTGGGCGCTTCG‑ACATCCGGTGTGCTTTTGCTTAACGGAAC‑AATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCAT  >  NC_000913/4570948‑4571419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A