Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F17 I0 R1 115 121.7 2441348 96.4% 2353459 231.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,151,320 T→G 20.7% H593P (CAT→CCT)  ppc ← phosphoenolpyruvate carboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,151,3200TG20.7% 87.0 / 2.0 48H593P (CAT→CCT) ppcphosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base T (26/8);  new base G (3/8);  total (32/16)
Fisher's exact test for biased strand distribution p-value = 8.52e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAGCCGCGGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGT  >  NC_000913/4151085‑4151554
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           
gtaGCCGCGGTAGACAGCGCAGGAGATGACTGACAGTTCCAGCATAATGCGTCGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCGCCGGTATAAAGCGACAGGCTGCTGACGGGGATTTCTGGCAGACCGTATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGACGCCTTTCAGG‑C‑TTCCTGGGGGTTGTGACAGCAGCG‑‑CCGCATGAg                                                                                                                                                                                                                                          <  2:957535/239‑1 (MQ=255)
 taGCCGCGGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGGCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAACAGCG‑‑CCGCATGGGc                                                                                                                                                                                                                                         >  1:1107575/1‑239 (MQ=255)
  aGCCGCGGTAGACATCGCAGGTGATGACTGACAGTTCATCCATAATGCTACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGc                                                                                                                                                                                                                                         <  2:592664/238‑1 (MQ=255)
                tCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCcgcg                                                                                                                                                                                                                          <  1:725684/239‑1 (MQ=255)
                           gactgacAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCCGCG‑‑CCCCAGGAGCAGGTGCGCCCCCGCGACCAAAGGac                                                                                                                                                                                                                >  2:1130025/1‑237 (MQ=255)
                           gactgacAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCCTGAGCAGGTGCGCCGCCGCGACCCATGGaa                                                                                                                                                                                                                >  1:421465/1‑238 (MQ=255)
                                                                 tCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGCTTTGACAGCACCG‑‑CCGCCGGAGCCGTTGTGCCCCCGCGACCCATGCAACCGCCGCGACCGTGGGATAACggccagtccacaccccca                                                                                                                                                                         >  2:149569/1‑222 (MQ=255)
                                                                 tCGGCTCCGGCGGGGGCAGCAGGTTGGCTTCCAGACTCGCCCCCGTATAAAGCGACAGGCGGCTGACGGTGATTTCTGGCAGACCCTATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTAAGG‑C‑TTCCTGGCGGTTGTTAGCGCACCG‑‑CCCCACGTGCCGGTGCGCCCCCCCGACCCATGGACACGCCCGGCGCGTGGGACGACGtgcccccccaaaccc                                                                                                                                                                           >  2:607826/1‑223 (MQ=255)
                                                                        cgggggGGGCAGCAGGTTGGCTGCCAGAACCGCCGCGGTAAAAAGCGACAGGTTGCTGGCGGTGATTTCTGCCAACCAATTTTAAAAGGGGTCCATCTCGCCTTGTC‑CGTTCGCGCGCGGGCCTCCTGTCGGG‑C‑TCCTGGGCGGTGGTGACGGCGGCG‑‑CCGCAGGAGCAGGGGCCCCGCCGCGCCAAAGGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGc                                                                                                                                                                  <  2:193737/239‑1 (MQ=255)
                                                                                                       ggcccGGTATAAAGCGACAGGCTGCTGACCGGGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCAGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGATAGAAGCC‑‑CCGCCCGAGCACGCGTGCCGCCGCGACCAATGGAACCGGCGCGACCCTCGAACAACCTCAGCTCACACCCCCCGTTTTCCCAGGTTTTCGTTAATGCACACGGTGACTGaca                                                                                                                                   >  2:796990/3‑237 (MQ=255)
                                                                                                         cccggtagaagggcgcgggagggtgAGGGTGATTTGTCGTAAACCATATGAAAAGCGGTTAGCCCCGCCCTGTT‑GGTGTGGGCGCGGGCCG‑CTTTCGGG‑CGTTCGGGGCGGTGGGGAGAGCGGCG‑‑CCGCCGGGGCGGGGGCGCCGCCGCGACCAGTGGATCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATAtt                                                                                                                                 <  2:118501/216‑1 (MQ=255)
                                                                                                                   aGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGCGCGCCCCCGCGACCAATGGAACCGCCGCGAACGGGGGACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCCACCTGAGCCTGATATTGCGCCCCGGa                                                                                                                       >  1:324288/1‑239 (MQ=255)
                                                                                                                       aCAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCCATGGAACCGCCGCGACCGCGGCACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGaa                                                                                                                      >  1:535407/1‑236 (MQ=255)
                                                                                                                       aCAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGGGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCCCGGCCGTGGAACAACGTCAGCGCAATACCCGCTTTTTCGCCGGGTTTGCTTAATGCATCCTGGGCCCGGTACTGCGCCCAAGaa                                                                                                                      >  2:1119901/1‑236 (MQ=255)
                                                                                                                        cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCCTGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCCGTGCCTGATATTGCGCCCCGGAAGCt                                                                                                                   >  1:319136/1‑238 (MQ=255)
                                                                                                                        cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCCGGAAGCt                                                                                                                   >  1:1057695/1‑238 (MQ=255)
                                                                                                                        cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGCTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCCCGACCCTGGAACAACGTCAGCCCAATACCCGCTTTTTCGCCGGGTTTGGTTAATGACACCTGTGCCTGATATTTCGCCCAGGAAGCt                                                                                                                   >  2:485535/1‑238 (MQ=255)
                                                                                                                        cAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCCGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGGTGTGACAGCAGCG‑‑GCCCAAGACCAGGTGAGCCCCCCCGACCAATGGAACCGCCGCGACCGCGGCACAACCCGAGCCCACCACCCCCTTTTTCGCCGGTTTTGTTTCGTGCATCCTGCGCCTGACATTCCGCCCCGGCAGCt                                                                                                                   >  2:569385/1‑238 (MQ=255)
                                                                                                                                     gTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCg                                                                                                     >  1:1051492/1‑238 (MQ=255)
                                                                                                                                                              aGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCCCGACCCATTGAACCGCCGCGACCGTGGGACAACGTCAGCTCCATACCCGCTTTTTCGCAGGCTTTGCTTAATGCATCCTGTGCCTGATCTTGCGCCCCGGACGCTC‑CCATCACCCCCCCCTCTTTTGCTTCTTCGGAACAgtcc                                                                           >  2:1156121/1‑236 (MQ=255)
                                                                                                                                                               gCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTTGCGGGTGTGATAGCAGCG‑‑CCGCATGGGCAGGTGCGCCCCCGCGGCCCATTGGACCGCCGCGAACGCGGAACCACGTCAGCTCCATACCCGGCTTTTTGCAGGGTTTGATTAATTAATCCTGTGCCTGCTATTGCGCCCAGGACGATGCCCTTCAC‑CCCCCATCTTTTTCTGCGTCGGACTAGCCaa                                                                          >  2:829247/1‑239 (MQ=255)
                                                                                                                                                               gCGGATCATCTCGCCCTGTT‑CGGTTA‑GCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCCGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGGAGCTG‑CCCTCACTCCCCCCTCTTTTGCTGAGTCGGACTTGCCaa                                                                          >  2:169911/1‑238 (MQ=255)
                                                                                                                                                                   atcatcTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGAt                                                                                                                                                        >  1:703679/1‑158 (MQ=255)
                                                                                                                                                                     ccccgccgccTGTTCCGGCTACGCGCGGGCCCCTGGTCGGG‑T‑CTCCGGGCGTTTGAGACCGCACCG‑‑CGGCATGGGCGGGTGCGCCGCCGCAACCAATGGAGCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAatcat                                                                      <  2:319136/230‑1 (MQ=255)
                                                                                                                                                                                gTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCCGGTGCGCCGCCCCGACCAATGGAACCGCCGCGACCCTGGAACAACGCCAGCTCAATACCCGCTGTTTCGCAGGTGTTGATTAATGGATCCTGTGCCTCAAATTTCCCCCAGGAAGCTT‑GCATCACTCCCGCATCTTTTGCTTCGTCTGAATCACCAATCAACACCATCTCttt                                                          >  2:1167422/1‑238 (MQ=255)
                                                                                                                                                                                 tt‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCCGGTGCGCCGCCGCGACCAATGGAACCCCCGCGCCCCTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGGTTTGATTAATGCACCCTGTGCCTGATATTGCGCCCAGGGAGCTG‑CCATCACTCCCCCATCTTTTTCTGCGTCGGGATAGCCAATCATCCCCCTCCGtttt                                                         >  2:7353/1‑237 (MQ=255)
                                                                                                                                                                                          gcgcgcggCCGCCTTTCTGGTC‑TCCCGGGCGTTAGTGAAGGCAGCG‑‑CCGCATGAGCGGGTGCGCCCCCGCCACCAGTGAAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAAt                                                 <  1:941807/233‑1 (MQ=255)
                                                                                                                                                                                          cgcgCAGGCCGCCTGTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGCGCAGGTGCGCCGCCCCGACCAATGGAACCGCCGCGGCCGTGGAACAACGTCCGCTCAATACCCGCGTTTTCCCAGGTTTTGATTAATGCATCCTGTGCCCGAGATTGCGCCCAAGAAACTG‑CCCTCACTCCCCACTCTTTGTCTTCCTCCGGATAGCCAAATATCTCCAACCGCTTGCCCtcactc                                                >  2:1104649/1‑234 (MQ=255)
                                                                                                                                                                                            cgcAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTTTGAAAGAAGCG‑‑CCGCCTGAGCAGGTGCGCCGCCTCGACCAATGGGACCGCCGCGCCCGTGGAACAAAGTCAGCTCAATACCCGCTTTTTCGCAGGCTTTGATTAATGCAGCCTGTGCCTGAAATTGCGCCCACGAAGCTG‑CCATTTCTCCCGCATCTTTTGCTGGGGAGGAATAGCCAATCATCACCATCTCTTTGCCCTGACTcg                                               >  2:348729/1‑237 (MQ=255)
                                                                                                                                                                                            cgcAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTTCTGAGTCGGCATAGCCAATCATCACCATCTGTTTGCCCTGAATCAg                                              >  1:783427/1‑239 (MQ=255)
                                                                                                                                                                                            cgcAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAg                                              >  1:960460/1‑239 (MQ=255)
                                                                                                                                                                                            cgcAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCCGGACCAATGGAAAAGCCGCGACCGTGGAAAAACGTCCGCTCAATACCCGCTTTTTAGCAGGGTTTGATTAATGCATCCTGTGCCTGATATTTCGCCCCGGAAGCTG‑CCATCACTCCCGCATCTCTTGCTTGGCAGGGATAGCCAATCATCACCACCTGTTTGCCCTTCATCAg                                              >  2:265052/1‑239 (MQ=255)
                                                                                                                                                                                            cgcAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAACG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAg                                              >  1:101615/1‑239 (MQ=255)
                                                                                                                                                                                              cAGGCCGCC‑TTCGCG‑C‑TTCCCAGCGGTT‑TGGCACCCGCGCCCCGCGTGAGAAGGGCCCCCCCCGCAACCAGTGGAGCCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGc                                            <  1:1155217/239‑1 (MQ=255)
                                                                                                                                                                                                 gccgccTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCCGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCCATACCCGCTTTTTCGCAGGTTTTGATTAATGCAACCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCCTTTTCTGAGGCGGGATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCAc                                         >  2:454965/1‑239 (MQ=255)
                                                                                                                                                                                                     ccTTTCGGG‑C‑TTCCTGGGGGTTGTGACAGCAGCG‑‑CCGCAGGAGGAGGTGCGCCGCCGCCACAAAGGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATa                                     <  1:1075312/239‑1 (MQ=255)
                                                                                                                                                                                                     ccTTTCAGC‑C‑TCCCTGGCGTTGGTGACAGCAGCG‑‑CCGCGGGACGAGGTCCCCCCCCGACACCAATGAAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATa                                     <  1:1119901/239‑1 (MQ=255)
                                                                                                                                                                                                     ccTTCCAGG‑T‑TTCCGGGCGGTTGTGCAAGCGGCG‑‑CCGAATGCGCAGGGGCGCCGCCGCGACAAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATa                                     <  1:862490/239‑1 (MQ=255)
                                                                                                                                                                                                          cAGG‑C‑TTCGTGGCGGTTGTGACCGCGGCG‑‑CCGCAGGAGCAGGTGCGCCGCCGCCACAAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGt                                <  1:1096927/239‑1 (MQ=255)
                                                                                                                                                                                                            cagcttgcgtGGCGGTTGGGACAGCAGCC‑‑CCGGAGGAGGAGGTGCCCCGCCCCACCCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGt                                <  1:380792/230‑1 (MQ=255)
                                                                                                                                                                                                                  tCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAatat                          >  1:447767/1‑239 (MQ=255)
                                                                                                                                                                                                                  tCCTGGCGGTTGGGACAGCAGCC‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCCCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCCGATATTGCGCCCAGGAAGCTG‑CCATCCATCCCGCCTCTTTTGCTGCGTCGGCATAGCCCATCATCACCATCTGTTTGCCCTGAATCCGGCCACGATACCAGTCAaaat                          >  2:821099/1‑239 (MQ=255)
                                                                                                                                                                                                                       gtggTTGTGACAGCGGCG‑‑CGCCAGGAGCAGGCGCGCCGCCGCGACCAAGGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGag                      <  1:130362/236‑1 (MQ=255)
                                                                                                                                                                                                                       gggtttGTGACCGCGGCC‑‑CCGCTTGGGCAGGGGCGCCGCCGCGACCATGGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGag                      <  1:665682/234‑1 (MQ=255)
                                                                                                                                                                                                                                    cagcG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCCTCTTTTGCTGAGTCGGAATAGCCAATCATCACCCTCTGTTTGCCCTGAATCAGGCCCCGATACCAGTCAATATTGAGCAGCTGGGTCATg         >  2:695683/1‑238 (MQ=255)
                                                                                                                                                                                                                                       cg‑‑ccgcAGGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGTTTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCAATCCCGCATCTTTTGCTGACTCGGAATAGCCAATCATCACCATCCTTTTGCCCTGAGTCAGGCCACGAAACCCGTCAATATTGAGAAGCTGGGTCATcaca      >  2:766419/1‑238 (MQ=255)
                                                                                                                                                                                                                                          cccgcAGGAGCAGGTCCGCCCCCACCCCAGTTGTATCCGCCGCGACCGTGGAACAACGTCCGCGCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCCTCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGAc       <  2:462281/235‑1 (MQ=255)
                                                                                                                                                                                                                                            cgcATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGt  >  1:503218/1‑239 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           
GTAGCCGCGGTAGACATCGCAGGAGATGACTGACAGTTCATCCATAATGCGACGCCAGCTCTCTTTCGGCTCCGGCGGTGGCAGCAGGTTGGCTTCCAGAATCGCCCCGGTATAAAGCGACAGGCTGCTGACGGTGATTTCTGGCAGACCATATTTAAAGCGGATCATCTCGCCCTGTT‑CGGTTACGCGCAGGCCGCCTTTCAGG‑C‑TTCCTGGCGGTTGTGACAGCAGCG‑‑CCGCATGAGCAGGTGCGCCGCCGCGACCAATGGAACCGCCGCGACCGTGGAACAACGTCAGCTCAATACCCGCTTTTTCGCAGGTTTTGATTAATGCATCCTGTGCCTGATATTGCGCCCAGGAAGCTG‑CCATCACTCCCGCATCTTTTGCTGAGTCGGAATAGCCAATCATCACCATCTGTTTGCCCTGAATCAGGCCACGATACCAGTCAATATTGAGCAGCTGGGTCATGACATCGT  >  NC_000913/4151085‑4151554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A