Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F18 I0 R1 274 115.5 2655328 86.7% 2302169 226.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 154,321 A→C 11.3% V369G (GTC→GGC)  htrE ← putative outer membrane usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913154,3210AC11.3% 280.4 / 3.6 101V369G (GTC→GGC) htrEputative outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base A (75/10);  new base C (2/14);  total (77/24)
Fisher's exact test for biased strand distribution p-value = 3.65e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCA  >  NC_000913/154086‑154557
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                
ggAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATTTCATCTTTTAAGAc                                                                                                                                                                                                                                                 >  2:393514/1‑237 (MQ=255)
     cacGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTACCAAACCAGCGGAATAGTTCTTA‑TCGGTCATCTGACTACCGG‑TATAA‑CCCGTCCGATAGTTATTCAGGCCGACGTAGTAGCTTGTATAAAATAACTTAGAGCAC‑CCCTGAATACCATATTTTCAGCACTGAcc                                                                                                                                                                                                                                           >  1:423869/1‑238 (MQ=255)
     cacGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGAcc                                                                                                                                                                                                                                           >  2:1177469/1‑238 (MQ=255)
          tAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCt                                                                                                                                                                                                                                     >  2:384438/1‑239 (MQ=255)
                tGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAATAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCCTCTTTTAAAACCTGACCGCCGCaaaaaa                                                                                                                                                                                                                                >  1:485417/1‑235 (MQ=255)
                       ggTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAAAATCATCTTTTAAGACCTGACCGCCGCTAAAATCCCaaact                                                                                                                                                                                                                        >  1:769259/1‑236 (MQ=255)
                             ttttATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑CATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGAAGTAGCTGGCTTGAACTAAATTAG‑GACTATCCTGAATACAATCTTTAAAGACCTGACACCCACTAAAAACCCAACGTCAAAc                                                                                                                                                                                                                   >  1:571284/1‑238 (MQ=255)
                                  tcatcCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAAAGTCCAAACCCAgg                                                                                                                                                                                                             >  1:56593/1‑239 (MQ=255)
                                       cGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCTAACGCCAGGGCGT‑a                                                                                                                                                                                                       >  2:1145730/1‑239 (MQ=255)
                                       cGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑a                                                                                                                                                                                                       >  2:694389/1‑239 (MQ=255)
                                       cGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑a                                                                                                                                                                                                       >  2:343515/1‑239 (MQ=255)
                                       cGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTAGAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCATGCTTACAATAAATTAG‑GCACATATTAAATACCATCTTTTAAACCCGCACCGCCCATAATCACCCAACGTCCAACCCCaagtggca                                                                                                                                                                                                        >  1:810195/1‑232 (MQ=255)
                                                    ttGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGaacaac                                                                                                                                                                                          >  2:140811/1‑239 (MQ=255)
                                                      agaagtggtaacacaaaaaaaaaaTACCACAAATAAAATATTAAAACAAAAACCTAACAACCCAGTGATATATTTATTA‑TGGGTTATCTGAATCCTGT‑TAAAA‑CCCGTAAAATATTTATTCAGGCCGTAGTATTAGCTTGCTAAAAAAAAATTAG‑TCTCACCCTGAATATCATTTTTTAAGACCTACCCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACgg                                                                                                                                                                                        <  1:760846/223‑1 (MQ=255)
                                                         aTGAGTCAAACCGAAAAAAAATGCCCCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCGGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGAt                                                                                                                                                                                      <  2:612699/238‑1 (MQ=255)
                                                                      aaagaaaATGCACCAACTGAAGAATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGGAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTACCAGCAGCTAATATCCAAACGACCAAC‑CCACAGCATAAACATTCGAAAAACGAATGAAAAAGA‑TTgaga                                                                                                                                                                       >  1:384591/1‑239 (MQ=255)
                                                                       aaaaaaaTGCCCAATCTGAATTATTCAGACAAAGACCTAACAACCCAGGGGTATATTTATTA‑TCGTTTATCGGAATCCGGG‑TATAC‑CCCTTCAGATAGTTATTCAGCCCTGTGTAGTAGCTTGCTTAAAATAAATTAG‑GCTCACCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCgag                                                                                                                                                                      <  2:1150194/236‑1 (MQ=255)
                                                                            aaTGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAACTAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGG‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATg                                                                                                                                                                  >  2:983658/1‑238 (MQ=255)
                                                                            aaTGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATg                                                                                                                                                                  >  2:355428/1‑238 (MQ=255)
                                                                                      cTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAAACCTGACCGCCGCTAATATCCCAACGTCCAACCCCAGGGCTT‑ACCATTTGAACACAGGTTGAGAAAGG‑TTGCGAGAATGTCCGCTTTGa                                                                                                                                                        >  1:759202/1‑238 (MQ=255)
                                                                                              ttCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCTG‑AAAATTTGAACAACGGTTAGGAAAGG‑TGGCGAGAAAGTCCCCTTTGGGCCCCCGGa                                                                                                                                               >  1:906568/1‑239 (MQ=255)
                                                                                                   aCCAAGCCCTAATAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑GATAA‑CCCCCCAGATAGTAATTCCGCCCGTAGCAGTAACTTGCTTGAAATAAATTAA‑GTGCATCCTGAAAATCATCTTTTAAGAACTGACCGCCGCTAATATCCCAACGTCCCACGCCAGGGAGA‑CACTATTCAACACACGAAAAGAAGGGATTGC‑AGAATGTCCGCtttaaaaccacgagtatc                                                                                                                                           >  1:1104409/1‑223 (MQ=255)
                                                                                                   aCCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGAttct                                                                                                                                           >  2:148882/1‑238 (MQ=255)
                                                                                                   aCCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCAGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGCCCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACACCGGATGCGAAAGG‑GTGAGAGAAATACACCTTTTGGCCAACGGAttctt                                                                                                                                          >  1:107752/1‑239 (MQ=255)
                                                                                                        gACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGCTg                                                                                                                                     >  2:118573/1‑239 (MQ=255)
                                                                                                          ccaaacaacCCGGGGGTATAG‑TATTAGTCGGTTA‑CTGAAATCCGGTTAAAC‑CCCGTAAGATAGTTATTCAGGCCGTAGTATTAGCTTGCTTAAAAAAAATTAG‑GCTCATCCTGAATACCATCTTTAAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGt                                                                                                                                   <  2:769259/236‑1 (MQ=255)
                                                                                                          ccaaacaacCCAGCGGTATAGTTATTA‑TCGGTTATTTGAATACCGG‑‑ATAACCCCGTAAAATATTTATTAAGGCCGTAGTAGTAGTTTGCTTAAAAAAAATTAG‑GCTCATCCTAAATACCATCTTTAAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGt                                                                                                                                   <  2:56593/236‑1 (MQ=255)
                                                                                                          ccaaaaaaaCCAGGGGTATTGTTATTT‑TGGGTTATCTAAATACCGT‑TAAAC‑CCCTTCAGATTGTTATTCAGGCCGTAGTAGTAGCTTGCTTAAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGt                                                                                                                                   <  2:541451/233‑1 (MQ=255)
                                                                                                          ccaaaCAAACCAGGGGTATAGTTATTA‑TCGTTTAGCTGAAACCCGG‑TATAA‑CCCGTAAATTAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAAAAAATTAG‑CCTCATCCTGAATATAATCTTTAAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGt                                                                                                                                   <  2:1240843/236‑1 (MQ=255)
                                                                                                          ccTAACAAACCAGGGGTATAGTTATTA‑CCGGTTATCTGAATACCGT‑TATAA‑CCCGTCAGATAGTTATTCAGGCCTTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTAAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGt                                                                                                                                   <  2:265501/239‑1 (MQ=255)
                                                                                                          ccTAACAAACCAGCGTTTTGTTTATTA‑GCGGTTATCTGAATACCGG‑TATAC‑CACGTAAGTTAGTTATTCAGGCCGTAGTAGTAGCTTGTTTGAAAAAAATTAG‑GCTCATCCTGAATACCATCTTTAAAGCCCTTACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGt                                                                                                                                   <  1:218280/239‑1 (MQ=255)
                                                                                                             aaCAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCga                                                                                                                                                                       >  2:543907/1‑200 (MQ=255)
                                                                                                             aaCAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGGGAATGTCCGCTTTGAGCCATCGGATTCTTCGTTGGTAAc                                                                                                                                >  2:218280/1‑239 (MQ=255)
                                                                                                             aaCAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAc                                                                                                                                >  2:161662/1‑239 (MQ=255)
                                                                                                             aaCAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGAGT‑AACATTTTAACAACCGATGAGAAAGG‑TTGCGACAATGCCCGCCTTGCGCCAtagagatctttgaaggtgaa                                                                                                                                >  1:1315617/1‑220 (MQ=255)
                                                                                                                       cGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCAGCTTTGAGCCATCGAATTCTTCGATGGTAAC‑AAAAAGAtg                                                                                                                      >  2:311751/1‑237 (MQ=255)
                                                                                                                                 atta‑tCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AAAAAGATCGCTTCCGTAc                                                                                                            >  2:742365/1‑238 (MQ=255)
                                                                                                                                    a‑tCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑CACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTg                                                                                                        >  2:73559/1‑239 (MQ=255)
                                                                                                                                    a‑tCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGGACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGAATCTTTGATGGTAAC‑AATAAGAACGCTGCCGTAACCTg                                                                                                        >  1:232512/1‑239 (MQ=255)
                                                                                                                                    a‑tCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTAcctga                                                                                                        >  2:1098803/1‑236 (MQ=255)
                                                                                                                                    a‑tCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCt                                                                                                         >  2:353970/1‑238 (MQ=255)
                                                                                                                                    a‑tCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AAAAAGATCGCTGCCGTACCCta                                                                                                        >  2:569003/1‑238 (MQ=255)
                                                                                                                                    a‑tCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCCATGGTAAA‑AAAAAAATGGCGTCCGAACCCta                                                                                                        >  1:1104657/1‑238 (MQ=255)
                                                                                                                                         cttttttgttatctctatttaaccccGTAAAATATTTATCCAGGCCGTATTGGTTGTTTTCTTAAAAAAAATTAG‑GCCCCGCATAAATATCTCTTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGAc                                                                                                      <  1:343515/216‑1 (MQ=255)
                                                                                                                                            atttaaatccgtg‑TATAA‑CCCGTCAGATATTTATCAAGGCCTTATTATTAGTTTGCTTAAAATAAATTAG‑GCCCACCCGAAATAACATCTTTAAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACt                                                                                                 <  2:485417/227‑1 (MQ=255)
                                                                                                                                             tCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCAATGGGAAC‑AATAAAATCGCGGCCGTACCCTTACGGACTc                                                                                                >  1:982598/1‑239 (MQ=255)
                                                                                                                                                        g‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGACTGTCCGCTTTGAGCCATCGGATTCTTCGAAGGTAAC‑AATAAAATAGCCGCCGTACCCTGAAGGagcaaaaacatgaaa                                                                                     >  1:597556/1‑225 (MQ=255)
                                                                                                                                                        g‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCTCTGCCGTACCCTGACGGACTCAGACCATAAAt                                                                                     >  1:1126649/1‑239 (MQ=255)
                                                                                                                                                            taA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGACCAACGGATGAGAAAGG‑TTGCGCGAATGGCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑ACTAGAAACGCTGCCGTACCCTGCCGAACCCAAatctgtttctta                                                                                  >  1:624613/1‑230 (MQ=255)
                                                                                                                                                            taA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTACAATCATCAATGAc                                                                                  >  1:526612/1‑239 (MQ=255)
                                                                                                                                                              a‑CCCTTCAGATAGTTATTCAGGCCATAGTATTAGCTTCATTGAAATAAATTAG‑CCACCTCCAAAATATCATTTTTTAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACAGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGa                                                                                <  2:1315617/239‑1 (MQ=255)
                                                                                                                                                                  cGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGaaa                                                                              >  2:373174/1‑238 (MQ=255)
                                                                                                                                                                   gTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCAATCGGATTCTTCGATGGTACA‑AATAAGATCGCTGCCGTACCCTGACGAACTCAAATCATCAATAACGaacg                                                                             >  2:135231/1‑236 (MQ=255)
                                                                                                                                                                    tCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCCCTTTGAGCCATCGGATTCTTCGA‑CGTAACAAATACAATCGCTGCCCTACCCTGCGGGAccaaaaacataaaagaggaaaac                                                                            >  1:861742/1‑216 (MQ=255)
                                                                                                                                                                     cAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGTCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACCCAGatccaaaaaaacaaaagag                                                                           >  1:573273/1‑222 (MQ=255)
                                                                                                                                                                     cAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAccg                                                                           >  2:453811/1‑238 (MQ=255)
                                                                                                                                                                        aTAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGCGAATGTCCGCTTTTAGCCATCGGATTCTTCGATGGTAAC‑AATAAAATCGCTGCCGTACCCAGACGGACCCAGATCATCAATGAACAAAggcccgg                                                                       >  1:283202/1‑233 (MQ=255)
                                                                                                                                                                            ttATTCAGCTCTTAGTAGTTGCTTTGTTTAAATAAATTAG‑GCTAATCCTTAATACCATTTTTTAAAACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTggcgg                                                                   <  2:851717/239‑1 (MQ=255)
                                                                                                                                                                              aTTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAAAGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AAAAAGAAGCCTGCCGTACCCTGACGGACTCCAACAAACAATGACGaaaagcccgggcgga                                                                  >  1:729276/1‑226 (MQ=255)
                                                                                                                                                                               ttCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGCTGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGGACCCTGCCGGACCCAGATCATCAATGAAGAAAGCGCCTGGCGGCa                                                                 >  1:837343/1‑238 (MQ=255)
                                                                                                                                                                                    gCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGTCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCACGAATATCCGCTTTGTGCCATCGGATTCTCAGATGGTAAA‑ACTAAGATCGCTCCCGCACCCTGAGGGACTCGTGTAATCAATAACAAACACGCCTgccgccccccccg                                                           >  1:425193/1‑230 (MQ=255)
                                                                                                                                                                                     ccGTAGTATGTGCTTGCTTGAAAAAAATTAG‑GCTCAACCTAAATATCATTTTTAAAGCCCGGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑Ccgtcg                                                          <  2:526612/238‑1 (MQ=255)
                                                                                                                                                                                            tgttggttgtttaAAATAATTTTG‑GCTCCTCCTGAATAACATTTTTAAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATaa                                                  <  1:643733/233‑1 (MQ=255)
                                                                                                                                                                                              tagCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATaa                                                  >  2:415362/1‑237 (MQ=255)
                                                                                                                                                                                              tagCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATAGCTGCCGTACCCTGACGGACTCAGATCATCAATGCAGAAAGCGCCTGGCGGCA‑CCGTGGTGTCAaaaaa                                                >  1:726860/1‑238 (MQ=255)
                                                                                                                                                                                                gcttgcttGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTTACGGACTCAAATAATCAATGACGAAAACGCCTGCGGGCA‑CCCTCGTTTCATAAATCt                                              >  1:1271135/1‑239 (MQ=255)
                                                                                                                                                                                                gcttgcttGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCCGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCt                                              >  2:1152393/1‑239 (MQ=255)
                                                                                                                                                                                                   tgcttgCAATCAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGGAAC‑AATAAGATCGCTGCCGTACCCACACGTACACAGATCCTAAATGACAAAAGGGCCGGCCGGCA‑CCGCCGTTTCATAAATCAtaa                                           >  1:747678/1‑238 (MQ=255)
                                                                                                                                                                                                   tgcttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AAt                                                                                                                            >  2:974187/1‑159 (MQ=255)
                                                                                                                                                                                                   tgcttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTtat                                           >  2:1321856/1‑239 (MQ=255)
                                                                                                                                                                                                   tgcttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGAC‑CCGTCGTTTCATAAATCTtat                                           >  2:468218/1‑239 (MQ=255)
                                                                                                                                                                                                   tgcttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAAATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACCAAAACGCCCGGCGGCA‑CCACCCTTTTATAAAACTtaa                                           >  1:966630/1‑238 (MQ=255)
                                                                                                                                                                                                   tgcttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTCCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGCTGGGAAC‑AATACAACCGCTGCAGTACCCTGACGGACCTGGATCATAAATAACGAAAACCCCTCACGGAC‑CCGCCCTTTCATaacatcaaa                                           >  1:1094109/1‑232 (MQ=255)
                                                                                                                                                                                                     cttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGTCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGTTAAC‑AATAAGATCCCTGCCGTACCCTGACGGACTCAGATCATCAATAACGAAAACACC‑GGCGGGACCCGCCGTTTCAAAAACCtataa                                          >  1:639137/1‑234 (MQ=255)
                                                                                                                                                                                                     cttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTtata                                          >  2:832816/1‑238 (MQ=255)
                                                                                                                                                                                                     cttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTtata                                          >  2:531274/1‑238 (MQ=255)
                                                                                                                                                                                                     cttgAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAA‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAACGCCGTGCGGCA‑CCGTCGATTCATaaaattcaaag                                         >  1:1001351/1‑231 (MQ=255)
                                                                                                                                                                                                          aataattTGG‑GCCCCTCCTAAATACCTTTTTTAAAGCCCGTACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCAcc                                    <  2:652273/239‑1 (MQ=255)
                                                                                                                                                                                                          aataaatTCG‑CCCCCCCCTAAATTTCATCTTTAAACCCCTGACCGCCGCTAAATCCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCAcc                                    <  1:373174/239‑1 (MQ=255)
                                                                                                                                                                                                          aaaaaatTAG‑GCTACGCCTGAATACCTTTTTTAAAGCCCGTGCCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCAcc                                    <  2:742449/236‑1 (MQ=255)
                                                                                                                                                                                                          aaaaaatGAG‑GCACATCCTAAAATTCATCTTTAAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCAcc                                    <  2:979/236‑1 (MQ=255)
                                                                                                                                                                                                           ataaatTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTTGATGGTAAC‑AATAAGATCGATGCCGTACCCTGACGGACTCAGGTAATCAATGACGAAAGCGCCTGGCGGAA‑CCGCCGTTTCAAAAACCTAAAAGCAAcca                                   >  1:648777/1‑238 (MQ=255)
                                                                                                                                                                                                           aaaaaatgaggctctcgttGAATATCACTTTTAAAGACCTGACCGCCGCTAATATCCCAACGGCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCAcc                                    <  2:571284/221‑1 (MQ=255)
                                                                                                                                                                                                                  aaaggaagccagaaaatccattTTTAAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTa                             <  2:1292615/221‑1 (MQ=255)
                                                                                                                                                                                                                     gCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGAACGCTGCCGTACCCTGACGGACTAAGATCCTCAATGACGAAATCGCCTGGCGGCA‑CCGTCTGTTCCTAAACATTATAAACCACTTGCGTAAtc                          >  1:742280/1‑238 (MQ=255)
                                                                                                                                                                                                                         aTCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAgg                        >  2:203833/1‑236 (MQ=255)
                                                                                                                                                                                                                               aaTATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGCAAC‑AATAAGATCGCTGCCGTACCCTGCCGGACTCAGATCATCAATGACGAAAGCGCCTGTCGGCA‑CCGTCGTTTCATAAACCTTATAGCCACCTTGCGTAATAGGGACATTgg                >  1:280299/1‑239 (MQ=255)
                                                                                                                                                                                                                                 tctcattTTTAAACACCTGCCCGCCGATAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCg              <  2:772872/237‑1 (MQ=255)
                                                                                                                                                                                                                                 tatCTTGTTTAAAGCCCGGCCCGCCGCTAATAACCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCg              <  2:1091778/239‑1 (MQ=255)
                                                                                                                                                                                                                                  acactcttgaaaGCCCGGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGt             <  2:1104657/229‑1 (MQ=255)
                                                                                                                                                                                                                                   tcatcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTCATAGAGACTTTGGCGtt            >  2:984765/1‑239 (MQ=255)
                                                                                                                                                                                                                                    catcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGTACTCAGATCATCAATGACGCAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGttt           >  2:698252/1‑238 (MQ=255)
                                                                                                                                                                                                                                    catcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTg           >  2:201119/1‑239 (MQ=255)
                                                                                                                                                                                                                                    catcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGGACCCTGACGGACTCAGATCATCATTGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGAAATAGTGAATTTGGGGTTg           >  1:954963/1‑239 (MQ=255)
                                                                                                                                                                                                                                     atcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGCCGTTTCATAAATCTTATAGCCACCTTCCGTAATAGTGACTTTGGCGTTgg          >  2:529967/1‑239 (MQ=255)
                                                                                                                                                                                                                                      tcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGCCGGACTCAGATCTTAAATGACGAAAGCCCCTGGCGGCA‑CCGTCGTTTCTTAAACCTTATAGCCACCTTGCGTAATAGTGACtt                   >  1:1292615/1‑229 (MQ=255)
                                                                                                                                                                                                                                      tcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGt         >  2:603247/1‑239 (MQ=255)
                                                                                                                                                                                                                                      tcTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACAGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGt         >  2:1168386/1‑239 (MQ=255)
                                                                                                                                                                                                                                         tttAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTAtt      >  2:1185763/1‑239 (MQ=255)
                                                                                                                                                                                                                                             aGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCa  >  2:1248965/1‑239 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                
GGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTA‑TCGGTTATCTGAATACCGG‑TATAA‑CCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAG‑GCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGT‑AACATTTGAACAACGGATGAGAAAGG‑TTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGATGGTAAC‑AATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCA‑CCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCA  >  NC_000913/154086‑154557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A