Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F18 I0 R1 274 115.5 2655328 86.7% 2302169 226.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 442,372 T→G 12.6% V275G (GTG→GGG)  thiI → tRNA s(4)U8 sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913442,3720TG12.6% 295.0 / 4.4 131V275G (GTG→GGG) thiItRNA s(4)U8 sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base T (62/45);  new base G (13/9);  total (77/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCG  >  NC_000913/442138‑442601
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        
tggtGCGTCGCGGCGGCCGTGTGTTTTACTGCTTCTTTACCCTCGGCGGCGCGGCGCATGAAAGTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCt                                                                                                                                                                                                                                      <  1:1071466/236‑1 (MQ=255)
 gATGCGTCGCGGCTGCCGCTTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGCCAGGTGGCGCCTTATCTGTGGAACCGCTTTGGCGGCCCCCACCGCGTGCGTTTTGTCGTTATTAATTTCGAACCGGTCGTCGGGGCAATTTCCGAGAAAATCCACGACGGTCATATGGGGG‑TTACCCTCAAACGTTTGAGTGTCCGGGCCG‑CA‑CTCAAAATGGCTg                                                                                                                                                                                                                                     >  1:100837/1‑239 (MQ=255)
  aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTTTCGCTATTAATTTCGAACCGTGCGTCGGGGAAATTCCCGAGAAAATCGACGACGGGAAGCTGGGGG‑GTATCCCCCAACGAATGATGGGCGGGGCCG‑CC‑TTCAAAGTggggga                                                                                                                                                                                                                                    >  1:467190/1‑235 (MQ=255)
  aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGGGCAAATCGACGACGTTCAAATGGGCG‑TTATCCCCAAACGTATGATGGTGCGTGCCGCCA‑TC‑AAAGTGGCtaa                                                                                                                                                                                                                                    >  1:222400/1‑237 (MQ=255)
  aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGCGAAAATCGCCGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAATGGGCTGa                                                                                                                                                                                                                                    >  2:369437/1‑239 (MQ=255)
  aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTCAAGTGGCTGa                                                                                                                                                                                                                                    >  2:705112/1‑239 (MQ=255)
  aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGGGGCTGa                                                                                                                                                                                                                                    >  2:201758/1‑239 (MQ=255)
  aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGCCAGATGGGCG‑TTATCTTAAAACGTATGATGGTGCGGGCAC‑CA‑CCAAAAGTGGCTGa                                                                                                                                                                                                                                    >  1:301061/1‑239 (MQ=255)
  aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCGTTGGCAGCCCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGCCGGGGAAATTCTCGGGCAAACCGCCGACCGTCAGATGGGCG‑TTTTTCCCAAACTGAAAATGGTGGGTGCCC‑CT‑CCTAAAAGGGCTGa                                                                                                                                                                                                                                    >  1:112084/1‑239 (MQ=255)
       tCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTAAGATGGTGCGTGCCG‑CC‑TCTAAAGTGGCTGCACGt                                                                                                                                                                                                                                >  2:53855/1‑238 (MQ=255)
       tCGCGGCTGCCGCGTGCATTACTGCTTATTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGCGCGTTTTTTCGCTATTGATTTTCAAACGGTCGTCGGGGAAATTCCCGGGAAAAGAGCCAACGGCCAGATGGGCGTTTTTCCTC‑AAGGTATAAGTGTGCGTGAGC‑CA‑TCCAAAATGGGTGAACAtt                                                                                                                                                                                                                               >  1:1198251/1‑239 (MQ=255)
          cgGCTGCCGCGGGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCCCGTGCGTTTTGTCGCTATTAATTTCCAACCGGTCGTCGCGGAAATTCTCAGTAAAATCTCCGCCGGTCAGCGTGGCA‑TTACCCCCAAACGATGGATCGTGCGTGCCG‑CA‑TCTAAAGTTGCTGAACGttcag                                                                                                                                                                                                                            >  1:141361/1‑236 (MQ=255)
            caggccggggtcATTTCTGCTTCCTTAACCCCGGCGGCGGGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGCCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACgg                                                                                                                                                                                                                           <  2:925258/227‑1 (MQ=255)
                     tGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGGCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑CTATCCTCAAACGTATGATGGTGCGTGCCG‑CC‑TCTCAAGTGGCTGAACGTTACGGCGAAC‑Agggg                                                                                                                                                                                                                >  1:860983/1‑237 (MQ=255)
                      gCATTACTTCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGgcg                                                                                                                                                                                                                <  2:319879/238‑1 (MQ=255)
                          tACTGCTTTTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGt                                                                                                                                                                                                           <  2:679426/239‑1 (MQ=255)
                          tACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCTCGTGCGTTTTGTCGCTATTAATTTCGCACCGGTCGTCGGGGAAATTCTCGAGACAATCGACGACGGCCAGATGGGCG‑TTATCCTTCAACGGATGGTGGTGCGTGCCG‑GC‑TCTAAAGGGGCTGAACGGTACGGCGACC‑AGgccccgg                                                                                                                                                                                                            >  1:925258/1‑233 (MQ=255)
                          tACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGCAGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CC‑TCTAAAGGGGCTGAACGTTAAGGCgacaagccgccgg                                                                                                                                                                                                             >  1:727544/1‑226 (MQ=255)
                          tACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGt                                                                                                                                                                                                           <  2:860983/239‑1 (MQ=255)
                               cttcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGGGGCGGAACGTTACGGCGTAC‑AGGGGCTGGTAACCg                                                                                                                                                                                                      >  2:1277455/1‑239 (MQ=255)
                                  cttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCg                                                                                                                                                                                                   >  2:1170413/1‑239 (MQ=255)
                                        ccgcggcggTGGGGCGCATGAAATTGGCGTGCGGCCGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGATATTAATTTCGAACCGGTCGACGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTTGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAgcgc                                                                                                                                                                                             <  2:695313/236‑1 (MQ=255)
                                         cTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTCA‑AGGCGCTGGTCACCGGCGAAgcgc                                                                                                                                                                                             >  2:798520/1‑238 (MQ=255)
                                            ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCTGCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAGACGTATGATGGTGCGAGCCG‑CA‑TTTAACGCTGCTGAACGTGACGGGCTAC‑AGGCGCTGGTCGCCTGCGAAGGGCTcc                                                                                                                                                                                          >  1:766868/1‑237 (MQ=255)
                                            ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGCGAAAATCAACGCCGGTCAGCTGGGCG‑TTATCCTCAAACGTATAATGGTGCTGGCCG‑CA‑TCTAAAGGGGCTGAACGTTACCG‑GTGCAAGGCGCCGGTCACCGGCGAACGGCTCgg                                                                                                                                                                                         >  1:1282806/1‑239 (MQ=255)
                                            ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGACCGTTACGTCGTAC‑AGGCGCCGGACACCGGCGAAGAGCTCg                                                                                                                                                                                          >  2:843852/1‑238 (MQ=255)
                                            ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCg                                                                                                                                                                                          >  2:646457/1‑238 (MQ=255)
                                            ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCGGGTCACCGGCGAAGCGCTCg                                                                                                                                                                                          >  2:438929/1‑238 (MQ=255)
                                            ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGCCGCTGGTCACCGGCGAAGCGCTCg                                                                                                                                                                                          >  2:687747/1‑238 (MQ=255)
                                            ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGGGCCG‑CA‑TCTAAAGGGGCTGAACGTTACGGCGTAC‑AGGCGCGGGTCACCGGCGAAGCCCTCg                                                                                                                                                                                          >  2:963282/1‑238 (MQ=255)
                                            ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACAGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGAATGATGGTGCGGGCCG‑CC‑TCTAAAGTTGCTGAACGTTACGGCGTAC‑AGACGCTGACCACCGCTGAAGGGCACg                                                                                                                                                                                          >  1:349620/1‑238 (MQ=255)
                                            ggcggcGCGGCGCATGAAATTGGCGGGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGCATGATGATGAGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGACGGAC‑AGACGCTGGGAACCGGCAAAGCGCTCg                                                                                                                                                                                          >  1:157954/1‑238 (MQ=255)
                                                 cgcggcgcATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGCCGACGGTCAGATGGGCG‑TTATCCTCAAACGAATGATGGTGCGTGCCG‑CA‑TCTACAGTGGCTGCACGTTACGGCCTAC‑AGGGGCTGGTCCCCGGCGAAGCGCCCGGCCggg                                                                                                                                                                                    >  2:569208/1‑239 (MQ=255)
                                                 cgcggcgcATGAAATTGGCGTGCGTCAGGAGACGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGCAATTCTCGAGAAAATCGACGACGGTCAGACGGGCG‑TTATCCTCAAACGTATGATGGCACGTG‑CG‑CATTCTCAAGGGGGCGAAAGTACCGGCGTTC‑AGCGGCTGGGCaacacgaaggaacgcggccggg                                                                                                                                                                                    >  1:613598/1‑218 (MQ=255)
                                                                   cgtgcgtCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGGCACCGCCGAAGCGCCCGGCCAGGGGTCCAGCCACACGcgg                                                                                                                                                                   >  1:1030518/1‑236 (MQ=255)
                                                                     tgcgtCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGTTCAGATGGGCG‑TTATCCTCAAACGCATGATGGTGCGTGCCG‑CA‑TCTAAATGGGCTGAACGTTACGGCGTAC‑AGGCGCTGTTCCCCGACGAAGCGCCCGGCCaaggggccacgcagaagggggcc                                                                                                                                                                >  1:507223/1‑217 (MQ=255)
                                                                     tgcgtCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGAc                                                                                                                                                                 <  2:1198428/238‑1 (MQ=255)
                                                                       cgtCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCa                                                                                                                                                               >  2:493694/1‑238 (MQ=255)
                                                                       cgtCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGACAATTCTCGAGAAAATCGACGCCGGTCAGATGGGCG‑TTATCCTCAAACGTATAATGGTACGTGCCG‑CA‑TCTAAATGGGCTGAACGTTACGGCGTAC‑AGGCGCGGGTCACCGGCGAAACGCTCGTGCAggggcccccccaaagccggacca                                                                                                                                                               >  1:1073303/1‑217 (MQ=255)
                                                                        gccgggTGGGGCTTTTTTTGTGGAACCGCTTTGTCAGCTCCCACCGCGTGGGTTTTGGCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGAGGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAAc                                                                                                                                                             <  1:569208/235‑1 (MQ=255)
                                                                               ggCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTgcgc                                                                                                                                                       <  2:112084/238‑1 (MQ=255)
                                                                                 cgcATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGACATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGGTGGAGGGTGCGTGCCG‑CA‑TCTAAAGGGGCTAAACGTTACGGCGTAC‑CAGCGCTGGTCACCGTCGAAGCGCTCGGCAAGGTGTCCAGCCCGaacaggcccaacaggggccag                                                                                                                                                    >  1:565989/1‑219 (MQ=255)
                                                                                 cgcATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGGGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTgctgtct                                                                                                                                                    >  2:506700/1‑234 (MQ=255)
                                                                                 cgcATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGGGTCCAGCCAGACGCTGACCAACCTccg                                                                                                                                                        >  2:1090023/1‑235 (MQ=255)
                                                                                  gcATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGGGCGTGCCG‑CA‑TCTAAAGTGGCTGACGGTTACGGCGAAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGCCCAGCCAGACGCTGACCAACCGGCGCCtga                                                                                                                                                   >  2:1153407/1‑239 (MQ=255)
                                                                                        tCTGTGGACCCGGTTTGGCAGCTCCCACCGGGTGGGTTTGGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGCCGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATa                                                                                                                                             <  1:923196/239‑1 (MQ=255)
                                                                                                  ccgttgggcgatttcccccGCGTGCGTTTTGTTAGATTTAATTTCGAGCCGGTCGTCGGGGAAATTCTCGAGAAAAACGACGCCGGTCAGATGGGCG‑GTATCCGCAAACGGATGAGGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCGCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCTCCTGATTGATAACGTCTC‑CGa                                                                                                                                  <  1:650217/222‑1 (MQ=255)
                                                                                                    cTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGGGTCCAGCCAGCCGCTGACCAACCTGCGCCTGATTGTTAACGTCCC‑CGac                                                                                                                                 >  2:569527/1‑238 (MQ=255)
                                                                                                      ttggccacccccACCGCGTGCGTTGTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGGGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACg                                                                                                                              <  2:473929/230‑1 (MQ=255)
                                                                                                      ttGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCCC‑CGccac                                                                                                                               >  2:281743/1‑238 (MQ=255)
                                                                                                        ggCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCt                                                                                                                            <  2:467190/239‑1 (MQ=255)
                                                                                                        ggCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCt                                                                                                                            <  2:672972/239‑1 (MQ=255)
                                                                                                               cccACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCGTCGCCTGATTGATAACGACTC‑CGACACGCTGACCCt                                                                                                                      >  2:809566/1‑238 (MQ=255)
                                                                                                                 ccccggttggtgtttGGCGTTATTAATTTCGCACTGGGGGCCGGGGAAATTCTCGAGAAAATCGGCGACGGTCAGATGGGCG‑TTATCCGCAAACGTATGATGGTGGGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCg                                                                                                                   <  1:798520/227‑1 (MQ=255)
                                                                                                                     ccgttcgttTTGGCGGTATTTATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGGATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCg                                                                                                               <  1:281743/238‑1 (MQ=255)
                                                                                                                                ttcccttttAATTTCAAACCGGTCGTCGGGGAAATTCCGGAAAAAATCGGAGGCGGTCAGATGGGCG‑TTAACCTAAAACGTATGATGGTGGGGGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTa                                                                                                    <  1:1236371/232‑1 (MQ=255)
                                                                                                                                     ttttAATTTGGAACCGGTCGTAGGGGAAATTTCGGAGAAAATCGGCCACGGTAAAGTGGGCG‑TTACCCTAAAACGTGGGATGGTGCGTGCCG‑AC‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACa                                                                                              <  1:201758/237‑1 (MQ=255)
                                                                                                                                     tATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGCCACCGGCGAAGCCCTCGACCAGGTGTCCAGCCCGACGCTGACCAACCCGCGCCGGATGAACAACGTGCC‑CGCCCCGCGAAACCTCCGCCCGCGGACCCCTTagaaaa                                                                                               >  1:412966/1‑234 (MQ=255)
                                                                                                                                     tATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAAAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACa                                                                                              <  2:1240419/239‑1 (MQ=255)
                                                                                                                                     tATTAATTTCGAACCGGTCGGCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑Ac                                                                                               <  2:719745/238‑1 (MQ=255)
                                                                                                                                      tttAGTTTCCAACGGGTGGGGGGGAAAATTGTCGGAAAAAGCGAGGACGGTCAGATGGGGG‑TTATCCTCAAAGTTATGGTGGTGCGTGCCG‑CA‑TCTAAAGGGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACa                                                                                              <  1:569527/237‑1 (MQ=255)
                                                                                                                                      aTTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACaa                                                                                             >  2:400672/1‑239 (MQ=255)
                                                                                                                                      aTTAATTTCGAACCGGGCGTCGGGGAAATTCTCGAGAAAATCGGCGACGGTCAGATGGGCG‑TTACCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACa                                                                                              <  2:100837/238‑1 (MQ=255)
                                                                                                                                            ttCGACCCGTTGGTCGGGGAAATTTCGGGGAAAACCGCGGACGGGCAGGTGGGCG‑TTATCCTCAAACGGATGATGGTGCGGGCCG‑CA‑TCTAAAGGGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGca                                                                                       <  1:646457/239‑1 (MQ=255)
                                                                                                                                            ttCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGca                                                                                       <  2:301061/239‑1 (MQ=255)
                                                                                                                                            tccGAACCGGTCGGGGGGGAAATTGTGGGGAAAAGCGACGACGGGCAGAGGGGCG‑TTAACCTCAAACGTAGGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGca                                                                                       <  2:188640/237‑1 (MQ=255)
                                                                                                                                                 aCCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAGCGGATGATGGTGCGTGGCG‑CA‑TCTAAAGGGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCAcatca                                                                                  <  2:609213/239‑1 (MQ=255)
                                                                                                                                                 aCCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGCTAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑CCAAAAAGCAcatca                                                                                  >  2:1147358/1‑239 (MQ=255)
                                                                                                                                                     gtcgtcGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGCTAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACAtcatca                                                                               >  2:791038/1‑238 (MQ=255)
                                                                                                                                                     gtcgtcGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑CCAAAGAGCACAtcatca                                                                               >  2:588168/1‑238 (MQ=255)
                                                                                                                                                     gtcgtcGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCGGCGCCTGATTGATAACGTCTC‑CGCAACCCTGATCCTCCGCCCCCTGATCTCttccacaaaaaacccaaccacca                                                                                >  1:718940/1‑217 (MQ=255)
                                                                                                                                                     gtcgtcGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAACCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGCCACGCTGATCCTGCGTCCGCTGTTCTCTTACG‑CCAAAGAGCACAtcatca                                                                               >  2:571736/1‑238 (MQ=255)
                                                                                                                                                                    ctcGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGAt                                                               <  2:1282806/239‑1 (MQ=255)
                                                                                                                                                                      cGAGAAAATCGACGACGGTCAGATGGGCG‑TTAACCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTg                                                             <  2:400883/239‑1 (MQ=255)
                                                                                                                                                                                            gtGGGGG‑TTTTCCCCAAACGTTTGATGGTGCGGGCCG‑CA‑TCTAAAGGGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCCCCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCa                                        <  1:58075/237‑1 (MQ=255)
                                                                                                                                                                                             tGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGGGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCAc                                       <  2:157954/238‑1 (MQ=255)
                                                                                                                                                                                             aatgtgtaaaagagaaagggTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCa                                        <  2:92732/219‑1 (MQ=255)
                                                                                                                                                                                                  g‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGCCACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCACCCTGGCCCGCCCGATTGGCACCGAAGACTTTGCTCGCCCGAGGcc                                 >  2:371183/1‑239 (MQ=255)
                                                                                                                                                                                                   tttATCCTCAAACGTATGATGGTGCGGTCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGc                                  <  2:565989/237‑1 (MQ=255)
                                                                                                                                                                                                    ttttCCTCAAACGTTTTGTGGGGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGGGTCC‑AGGGGCGGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCg                                <  1:400672/236‑1 (MQ=255)
                                                                                                                                                                                                     ttcccgaaaaCGTATGATGGCGCGGGGCG‑CA‑TCTAAAGGGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCg                                <  2:537426/231‑1 (MQ=255)
                                                                                                                                                                                                     tATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCg                                <  2:1030518/238‑1 (MQ=255)
                                                                                                                                                                                                       tCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGCCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCCACGTCCGCGGATCTCTTACG‑ACAAATGTCACATCATCAACCTGCCCCGCCAGATTGGCACCGAAGACCTTTCCCGCCCGAGGCCGGaa                             >  1:701298/1‑239 (MQ=255)
                                                                                                                                                                                                         cccatcctgttgaggGTGCTGGCCG‑CA‑GCTAAAGTGGCCGAGCTGTTCTGCCTAT‑AGGCGCTGGCCCCCGGCGAAGGCCTCTGCCAGGTGGCCATCCAGCCGCTGACCAACCTGCGCCTGATTGATCACGTCGC‑CGACACGCTGATCCCGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAata                           <  1:371183/229‑1 (MQ=255)
                                                                                                                                                                                                           cAAGCGGATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGTTGAACGATAGGGCGTAC‑ATGCGCTGGAAACCGACGCAGCGCTCGACCAGGTTGCGAGCCACACGCTGACAACCCTGCGCCTGTTTGATAACGTGTCACCACCCAC‑GGTCACGCGTCCGTTGACCTTTTTCG‑ACCAAGTGCACATCATTAACCTGGCCCGCCGGACGTGAACCCAAGACTTTCCtgccaccgatgcgcaacgat                         >  1:896655/1‑220 (MQ=255)
                                                                                                                                                                                                                  atgatgGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCTCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTgtggtg                   <  2:141361/238‑1 (MQ=255)
                                                                                                                                                                                                                   tggtgggtggtgcCT‑CA‑TATAAAGGGGGTGAAGCTTCCGGCAGAC‑AGGCGCTGTGCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCGGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGGGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGtgtg                 <  1:441471/230‑1 (MQ=255)
                                                                                                                                                                                                                   tgatgGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGCACGATTTTGGtgtg                 >  2:441471/1‑239 (MQ=255)
                                                                                                                                                                                                                   tgatgGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCCCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCCCCAGATTGCAACCGAAGACTTTGCTCGCACAAGTCCGGAAAATTGTGGtgtg                 >  1:356474/1‑239 (MQ=255)
                                                                                                                                                                                                                          gcgtgcCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACCATGCCGGAATATTGTGGGGTGATATCCa          >  1:216561/1‑239 (MQ=255)
                                                                                                                                                                                                                          gcgtgcCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGGCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCGGATCCTGCGCCCCCTGATCTCTTACG‑ACCAAGAGAACATCATCAACCTGGCCCCCCACATTGGCCCCGAAGACTTTGCTCGCACGATGCCGGaactgtcccttaaaaacccca          >  1:92732/1‑220 (MQ=255)
                                                                                                                                                                                                                           cgtgcCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGCCTTTGCTCGCACGATGCCGGAATATTGTGGGGTGATTTCCaa         >  2:722200/1‑239 (MQ=255)
                                                                                                                                                                                                                           cgtgcCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGCTGCCGGAATATTGTGGTGTGATCTCCaa         >  2:447093/1‑239 (MQ=255)
                                                                                                                                                                                                                           cgtgcCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGCATATTGGGGTGTGATCTCCaa         >  2:1094791/1‑239 (MQ=255)
                                                                                                                                                                                                                           cgtgcCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGCGTACAGACAGACGCTGACGAACCTGCGCCTGATTGATAACGTGTC‑CGACAAGCTGATCCTGCGTCTGCTGAGCTCTTACGCACAAAGGCCAC‑TCATCACACTGGCCCGCCAGCTTGGCCCCCAAGAATTTGTACCCACGATGCCGGATTATTatgggtggactccgca         >  1:809202/1‑227 (MQ=255)
                                                                                                                                                                                                                             tgcCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGCAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCaaa        >  2:254606/1‑238 (MQ=255)
                                                                                                                                                                                                                             tgcCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGACTCTTGTGGTGGGATCTCCaaa        >  2:410795/1‑238 (MQ=255)
                                                                                                                                                                                                                                   cA‑TTTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCg  <  2:222400/239‑1 (MQ=255)
                                                                                                                                                                                                                                   cA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCa                                        >  1:872869/1‑201 (MQ=255)
                                                                                                                                                                                                                                   cA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCa                                        <  2:872869/201‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        
TGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG‑TTATCCTCAAACGTATGATGGTGCGTGCCG‑CA‑TCTAAAGTGGCTGAACGTTACGGCGTAC‑AGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTC‑CGACACGCTGATCCTGCGTCCGCTGATCTCTTACG‑ACAAAGAGCACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCG  >  NC_000913/442138‑442601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A