Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F18 I0 R1	274	115.5	2655328	86.7%	2302169	226.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	538,368	A→G	13.6%	S246G (AGC→GGC)	ybbW →	putative allantoin transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	538,368	0	A	G	13.6%	194.1 / 2.0	81	S246G (AGC→GGC)	ybbW	putative allantoin transporter
Reads supporting (aligned to +/- strand): ref base A (5/61); new base G (10/3); total (16/65)
Fisher's exact test for biased strand distribution p-value = 4.85e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTTGGCGGTGGCAAAGTTTTAAATAAATTCACTGCCATTCTTAACCCGTGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTAT  >  NC_000913/538150‑538597

gTTTTGGCGGTGGCAAATTTTTAAAAAAATTCCCTGCCTTTTTTAACCCGTGCATCTATATCGTTTTCGGCCGTTTGGCGATTTGGGGCATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCa‑a                                                                                                                                                                                                                         <  1:776172/239‑1 (MQ=255)

  tttGGCGGTGGCAAAGTTTTAAATAAATTCACTGCCATTCTTAACCCGTGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGGGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGGGAGGGCATACG‑ACTTTATGCg‑aa                                                                                                                                                                                                                        >  2:159678/1‑238 (MQ=255)

    tGGCGGTGGCAAAGTTTTAAATAAATTCACTGCCATTCTTAACCCGTGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGTGGGCAACCG‑GCTTTatccgaaaa                                                                                                                                                                                                                       >  2:486850/1‑230 (MQ=255)

    tGGCGGTGGCAAAGTTTTAAATAAATTCACTGCCATTCTTAACCCGTGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACg                                                                                                                                                                                                                     <  2:1013631/239‑1 (MQ=255)

    gggCGGTGGCAAAGTTTTAAATAAATTCACTGCCATTCTTAACCCGTGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACg                                                                                                                                                                                                                     <  2:1270957/238‑1 (MQ=255)

      gccgttgaaaaTTTTTAAATAATTTCACTGCCATTCTTAACCCGTGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGTTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGcc                                                                                                                                                                                                                   <  1:1023457/231‑1 (MQ=255)

                      aaataaatTCACTGCCATTCTTAACCCGTGCATCTATATCGTTTTCGGCGGTAGGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGc                                                                                                                                                                                                 <  2:25850/239‑1 (MQ=255)

                            atTCACTGCCATTCTTAACCCGTGCATCTATATCGTTTTCGGCGGTATGGCGATTTTGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑CCTTTTTCCA‑AAAAACCCCCCC‑CA‑TTCGTGGGCAggggg                                                                                                                                                                                           >  2:1237512/1‑236 (MQ=255)

                             ttCACTGCCATTCTTAACCCGTGCATTTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCt                                                                                                                                                                                          <  2:1290798/239‑1 (MQ=255)

                             aaagggacctttCTTAACCCGTCCATTTATACCGTTTTCGGTGGTTTGGCGTTTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCt                                                                                                                                                                                          <  1:358703/229‑1 (MQ=255)

                                  tGCCATTCTTAACCCGTGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGGGGGCGCCTCCC‑CCCTTTCGCA‑CAAAGCCCCACC‑CT‑TTCGTGCGCAGGCGCTCGGGc                                                                                                                                                                                     >  2:942908/1‑239 (MQ=255)

                                  agCCTTTCCTAACCCGAGCATCTTTATCGTTTTCGGCGGTATGGCGATGTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGc                                                                                                                                                                                     <  2:1026647/238‑1 (MQ=255)

                                      aTTCTTAACCCGTGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGGCTGGGCGGCACCGGC‑GGTGGGCGCCACCC‑GCGTTACGCA‑AAAACCCCACACGGT‑TTCGTG‑GCCGACGCTGGGGG‑aaa                                                                                                                                                                                 >  2:1123839/1‑238 (MQ=255)

                                       ttCTTAACCCGTGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACg                                                                                                                                                                               <  2:638454/239‑1 (MQ=255)

                                       ttCTTAACCCGTGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATCCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACg                                                                                                                                                                               <  2:698045/239‑1 (MQ=255)

                                         ttttccccgggtgattgtatCGTTTTCGTGGGTGTGGCGTTTTGGGTGATTTCACGGGACGGGATCTGTCCAAGCTTTGACTACCTTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTATCGCGTTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCt                                                                                                                                                                             <  1:1138270/222‑1 (MQ=255)

                                         cTTAACCCGTGCATCTATATCGTTTGCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGGGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCt                                                                                                                                                                             <  2:957290/239‑1 (MQ=255)

                                            aaCCCGTGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCCATCTTTGACTACATTCCGAGCGGAATTCAGAAAGCAGAAAACAGGGGCTTC‑CTGTTCCTGGGGGTGATTAACGCGGTGGTTGCGGGCTGGGGGGCACCGTC‑GGTGGGGGCAGCCG‑CCTTTTCTGA‑GAAAACCCCCCC‑GC‑TTCGTGGGGAGGGGGTggggggaacccatgg                                                                                                                                                                           >  1:1045838/1‑228 (MQ=255)

                                                  tGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCGCCGGC‑GGTGGGGGCATCCG‑ACTTTTCTGA‑AAACGACCCCCC‑GC‑TTCGTGGGAAGGCGCTTGGGCAAAACGCGGGGttatg                                                                                                                                                                     >  2:44575/1‑236 (MQ=255)

                                                  tGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGCGGGGGGGGGCACCCG‑CTTTTAC‑CA‑CAAAGCCCAACC‑CC‑TTCGGGAGCCGGGGCCTGGGC‑GAACGCTGGGGTTTGt                                                                                                                                                                    >  2:372691/1‑239 (MQ=255)

                                                  tGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGGGGGCATTCG‑ACTTTTTCCC‑AAACACCCACCC‑GG‑TTCTGGAGCAGGCGCCgggggcaaccccctgcgctct                                                                                                                                                                     >  2:358703/1‑222 (MQ=255)

                                                  tGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCCCTC‑GT‑TTCGTGAGCAGGCGCGGGGGG‑AAACGCGGGGGTTAGt                                                                                                                                                                    >  2:226718/1‑239 (MQ=255)

                                                   gCATCTATATCGTTGTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCAAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGtt                                                                                                                                                                   <  2:618885/239‑1 (MQ=255)

                                                    cATTTATATCGTTTGCGGCGGTATGGCGATTTGGGCTATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGtt                                                                                                                                                                   <  2:1163568/238‑1 (MQ=255)

                                                     ctctttttctgtttggttgttttGGCGTTTTGGGCGGTTTGCCTGGGCGGGAGCGTTCCAATCTTTGCCTTCATTCCGGGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTTCTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGTCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTtgt                                                                                                                                                                 <  1:515281/217‑1 (MQ=255)

                                                     aTCTATATCGTTTTCGGCGGTATGGCAATTTGGGGGATTTCGCTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTtgt                                                                                                                                                                 <  2:669073/239‑1 (MQ=255)

                                                      ttttgatCGTTTTCGGCGGTTTGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTtgt                                                                                                                                                                 <  2:1045838/233‑1 (MQ=255)

                                                        tttttggTTTTGGGCGGTTTGGGGATTTGGGTGTTTTGACTGGTGGGGATCGGTCCAATCTTTGGCTTCATTCCGAGCGGTATTGAGAAAGCAGAAAACGGTGGGTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGc                                                                                                                                                              <  1:1226795/233‑1 (MQ=255)

                                                                 ttCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGGGAGGGGATCCC‑GCTTTTAGCC‑AAAAGCCCCCTC‑GT‑TTCGTGGGCGGGGGCTGGGGG‑CAAAGCCGGGGTTTGTTTTGGCCTATA‑tt                                                                                                                                                      >  2:31640/1‑237 (MQ=255)

                                                                             gtgttttGGGCGGTTTCACGGGTCGGGAGCGGTCCAATCTTTGATTACGTACCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CGGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCGGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCGCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGc                                                                                                                                       <  1:849663/235‑1 (MQ=255)

                                                                             gggATTTGGGCGGTTTGCCTGGGCGGGATCGGTCCAATCTTTGGCTTCAGTCCGAAGGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGGGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGGGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGc                                                                                                                                       <  1:234386/237‑1 (MQ=255)

                                                                              cGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGc                                                                                                                                       <  2:1225983/238‑1 (MQ=255)

                                                                              cGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGc                                                                                                                                       <  2:831401/238‑1 (MQ=255)

                                                                               gATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGcc                                                                                                                                      <  2:964244/238‑1 (MQ=255)

                                                                               gATTTGGGCGATTTCACTGGTCGGGATCGGTCCAACCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGcc                                                                                                                                      <  2:654691/238‑1 (MQ=255)

                                                                               gATTTGGGCCATTTCCCTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGcc                                                                                                                                      <  2:804927/238‑1 (MQ=255)

                                                                                aTTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGGGCGCATCCG‑CCTTTTCGCA‑AAACGCCCCCCC‑GT‑TTCGTGGGCGGGGGCCGGGGG‑CAACCCGGGGGTTTTTTTTGGGCTTTA‑TTTTT‑TTTTCggggcccgg                                                                                                                                    >  2:290833/1‑232 (MQ=255)

                                                                                  ttGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCggg                                                                                                                                   <  2:432489/238‑1 (MQ=255)

                                                                                           ttCCCTGGTCGGGATCGGTCCAATCTTTGACTACCTTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGtatta                                                                                                                         <  2:602644/239‑1 (MQ=255)

                                                                                           ttCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATGCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGtatta                                                                                                                         <  2:438508/239‑1 (MQ=255)

                                                                                               cTGGTCGGGATCGGTCCAATCTTTGACTTCATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑Gc                                                                                                                    <  2:1312539/239‑1 (MQ=255)

                                                                                                 ggTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGcc                                                                                                                                                                                                                   <  2:692060/148‑1 (MQ=255)

                                                                                                       gATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGtt                                                                                                                                                                        <  2:450674/182‑1 (MQ=255)

                                                                                                        ttcgtttcAATCTTTTTCTTCTTTCGGGGTGTTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTGGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCa                                                                                                            <  1:31640/231‑1 (MQ=255)

                                                                                                        aTCGGTCCAATTTTTGACTACATTCCGGGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCa                                                                                                            <  2:717735/238‑1 (MQ=255)

                                                                                                        aTCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCa                                                                                                            <  2:499145/238‑1 (MQ=255)

                                                                                                        aTCGGTCCAACTTTTGACTTCATGCCGGGCGGTATTCAGAAAGCAGAAAACGGTGGATTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCCCCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCa                                                                                                            <  2:460375/238‑1 (MQ=255)

                                                                                                            gTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGGGGGGGCCTCCGCACTTT‑CGCACAAACCCCCACCC‑TG‑TTCCGGTGC‑GGCGCTCGGGCGCAACACGGGGTTTATTTGGTGCCCAT‑‑TTCTGTTTTCCGCGCGCGGGGGGGTGTTTTTTT‑GCCgggccaaggat                                                                                                        >  1:770094/1‑230 (MQ=255)

                                                                                                             tCCAATCTTTGCATACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTAtt                                                                                                       <  2:356832/238‑1 (MQ=255)

                                                                                                                    tttGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCGGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACgg                                                                                               <  2:982880/239‑1 (MQ=255)

                                                                                                                      tGACTTCATTCCGAGCGGTATTCAGAAAGCAGAAATCGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGgcg                                                                                             <  2:755812/239‑1 (MQ=255)

                                                                                                                      tGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGgcg                                                                                             <  2:156691/239‑1 (MQ=255)

                                                                                                                       gACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGGGCGCCTACG‑ACGTTTCCCA‑AAACCCCCACCC‑GT‑TTTGTGAGCCGGCCGCGGGGC‑CAACGCTGGGGTGTGTTGTGGTC‑ATATTTCTT‑TTTTCGGGCTCCGGGGGTGTTTTTTTT‑GCCGGGGCCAGTAtgacatacggggg                                                                                            >  1:1300668/1‑227 (MQ=255)

                                                                                                                        gcTACATTGCGGGCGGTTTTCAGAAAGCGGAAAACGGTGGCTTC‑CTGGTCCGGGTGGTGATGAACGCGGTAGTTGCGGTCTGGGCGGGACCGGG‑GGGGGGCGCATCCG‑ACTGTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCt                                                                                           <  1:618828/238‑1 (MQ=255)

                                                                                                                        aCTTCATTCCGAGCGGTAATCAGAAAGCAGAAAACGGTGGCTTC‑CTTTTCCGGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCt                                                                                           <  2:551436/239‑1 (MQ=255)

                                                                                                                              ttCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTtgt                                                                                                                                                                 <  2:332112/166‑1 (MQ=255)

                                                                                                                                  tgtgggTTTTCTGAAGGCAGAACGGTGTGGTTTT‑CTTTGCCTGGGGTGGTTTAACGCGTTAGTTGCGGTCTGGGGGGCACGGGG‑GGTGAGCGCCTCCG‑ACTTTACGCA‑AAACGCCCTCGC‑GT‑GTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGa                                                                                 <  1:4167/235‑1 (MQ=255)

                                                                                                                                  ggggggTATTCAGAAAGCAGAAAACGGTGGCTTG‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGa                                                                                 <  1:290833/235‑1 (MQ=255)

                                                                                                                                  gagggtttttaaGAAAGCAAGAACCGGGTGCTTA‑CTGTTCCTGGTTGTGTTTAACGGGGTAGCTGGGGGCTGGGGGGCCTCGGC‑GGGGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGTTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGGTGATTCCTGGa                                                                                 <  1:1123839/228‑1 (MQ=255)

                                                                                                                                       agaTTCAGAAAGCAGAAAGCGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTg                                                                            <  2:696784/237‑1 (MQ=255)

                                                                                                                                        tATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGa                                                                                 <  2:488284/233‑1 (MQ=255)

                                                                                                                                         tttggaaaggCAGAATACGGGGGGTTCGCTGTT‑CTGTGGGGGATTAACGCGGTGGTGGCGGCCTGGGCGGCTGCGGT‑GGTGTGCGCATCCG‑ACTTGACGCA‑AACAGCCCTCTC‑GT‑TTCGTGAGCAGGCGGTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TGCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTAGTCACTACGGCGCTGATACCTGGAACGTGCt                                                                          <  1:1191453/230‑1 (MQ=255)

                                                                                                                                         aTTCAGAAAGCAGAAACCGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCt                                                                          <  2:1009388/239‑1 (MQ=255)

                                                                                                                                          ttCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCt                                                                          <  2:926472/238‑1 (MQ=255)

                                                                                                                                                  gCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGGGCGCATCCG‑ACTTTACGCA‑AAACGCCCCCTC‑GT‑TTCTTGAGCAGGCGCCGGGGG‑AAACGCCGGGTTTTGTTGTGGCCCATT‑TTTTG‑GTTGCGGGCCCCGGGGGATGGATTTTTGGCGGGGGCCAGATTTAACTA‑GGCGCGGAGAACTGGaaagagggtgagcgggt                                                                 >  1:488284/1‑224 (MQ=255)

                                                                                                                                                    aaaaccgggtggtTCC‑CTG‑TCCCGGGGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTc                                                               <  2:921632/231‑1 (MQ=255)

                                                                                                                                                       aaccGGGGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGG‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGGCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTc                                                               <  2:1271639/233‑1 (MQ=255)

                                                                                                                                                       aaaCGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCCCCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTc                                                               <  2:861261/236‑1 (MQ=255)

                                                                                                                                                       aaaCGGTGGCTTC‑CTGTTCCTGGGGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTc                                                               <  2:1290329/236‑1 (MQ=255)

                                                                                                                                                                  tc‑ctgttccggGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGA‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑a                                                                                                                                                                                   <  2:780627/112‑1 (MQ=255)

                                                                                                                                                                       gttcctgGGGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGt                                             <  2:1211326/239‑1 (MQ=255)

                                                                                                                                                                        tacctgGTGGTGATTAACGCGGTAGTGGCGGTCTGGGCGGCACCGGG‑GGTGAGCGCATCCG‑ACTTGACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGGGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGt                                             <  2:1324627/236‑1 (MQ=255)

                                                                                                                                                                                      tttcgcgGTTTTTGGGGTTTGGGCGGCGCCGGC‑GGGAAGTCCATTCG‑TCTTTACGCA‑AAACGCCCTATT‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGTGACAGCCTGTTCGCCTCGTTCTTTg                              <  1:226718/236‑1 (MQ=255)

                                                                                                                                                                                      tAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTg                              <  2:770094/239‑1 (MQ=255)

                                                                                                                                                                                          gcgGGAGTTGCGGGCGGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGt                          <  2:729781/239‑1 (MQ=255)

                                                                                                                                                                                              tAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAAAGCCCCCTC‑GT‑TTCCGGAGCAGGCGGTGGGGG‑AAAACCCGGGGTTAGTTGTGGGCCTTA‑TTTTG‑TTTGGGGGGCGCGGGGGGTGCGTTATT‑TCCGGGGCCAGGTATCCCCACGGCGGCGGCGCCCGTGAGGGGCTGGGTATTGTTTTGCGGTTGGGCAGCCCTTGTGCCTCGTTCTTTGTGGTTCTg                      >  1:1025182/1‑239 (MQ=255)

                                                                                                                                                                                                ggtggcgTCTGGGCGGCTCCTGG‑GGGAGGCGTCTCCG‑TCTTTACGC‑‑AAACCCCCCCTT‑GTGTTCGTGAGCAGGTGCTGGGTG‑AAACGCTGGGTTTAGTTGTGGCCTATA‑GTCTG‑TTTGCTGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATCCCTGGAACGTGCTGGATATTGTTCAGCTTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTgg                     <  1:44575/232‑1 (MQ=255)

                                                                                                                                                                                                     gggcggggCGGCCCCGGC‑GGGGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCTCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTAtt                <  2:742853/233‑1 (MQ=255)

                                                                                                                                                                                                         tGGGCGGCGCCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCtgat           <  2:585065/239‑1 (MQ=255)

                                                                                                                                                                                                                 aCCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACt    <  2:1069698/238‑1 (MQ=255)

                                                                                                                                                                                                                  ccGGG‑GGTGGGGGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTAt  <  2:1025182/239‑1 (MQ=255)

GTTTTGGCGGTGGCAAAGTTTTAAATAAATTCACTGCCATTCTTAACCCGTGCATCTATATCGTTTTCGGCGGTATGGCGATTTGGGCGATTTCACTGGTCGGGATCGGTCCAATCTTTGACTACATTCCGAGCGGTATTCAGAAAGCAGAAAACGGTGGCTTC‑CTGTTCCTGGTGGTGATTAACGCGGTAGTTGCGGTCTGGGCGGCACCGGC‑GGTGAGCGCATCCG‑ACTTTACGCA‑AAACGCCCACTC‑GT‑TTCGTGAGCAGGCGCTGGGGC‑AAACGCTGGGTTTAGTTGTGGCCTATA‑TTCTG‑TTTGCGGTCGCCGGGGTATGTATTATT‑GCCGGAGCCAGTATTCACTACGGCGCTGATACCTGGAACGTGCTGGATATTGTTCAGCGTTGGGACAGCCTGTTCGCCTCGTTCTTTGCGGTACTGGTTATTCTGATGACAACTAT  >  NC_000913/538150‑538597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A