Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F18 I0 R1 274 115.5 2655328 86.7% 2302169 226.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 649,330 A→C 11.5% V81G (GTG→GGG)  citF ← citrate lyase, citrate‑ACP transferase (alpha) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913649,3300AC11.5% 271.1 / 3.9 112V81G (GTG→GGG) citFcitrate lyase, citrate‑ACP transferase (alpha) subunit
Reads supporting (aligned to +/- strand):  ref base A (64/30);  new base C (6/11);  total (70/42)
Fisher's exact test for biased strand distribution p-value = 1.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

ATTCAGTTCGCCGCTCTGTACCAGA‑TGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGTTCTTGTCGTTGAGATTGTTCAATTTT  >  NC_000913/649095‑649562
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        
atTCAGTGCGCCGCTTTTCCCCAGA‑GGCACGCGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCa                                                                                                                                                                                                                                       <  2:266864/239‑1 (MQ=255)
atTCAGTCCGCCGCTCTGAACAAGA‑TGCCCACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCa                                                                                                                                                                                                                                       <  2:309971/239‑1 (MQ=255)
 ttCAGTTCGCCGCTCTGTACCAGA‑TGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑GGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCCGGGTCAGGTTTTTTAAGCCCATCTTCCCC‑ATGACGGACCTCACCaa                                                                                                                                                                                                                                      >  1:1249783/1‑238 (MQ=255)
   cactccccggcTCTGTCCAAGA‑TGCCCCCGACCGCCGTGAGAGTGGATCGTCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCatat                                                                                                                                                                                                                                    <  2:412876/230‑1 (MQ=255)
     gtccgccgcTTGGTCCGAGA‑TGCCCCCGACCGCCGGGAGAGTGGATCTGCCCCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTg                                                                                                                                                                                                                                  <  2:414905/236‑1 (MQ=255)
     gctCGCCGCTCTGT‑CCGGATTGCCCCCGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTg                                                                                                                                                                                                                                  <  2:243112/237‑1 (MQ=255)
      ttCGCCGCTCTGTACCAGA‑TGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑AGGCCGTCCATCAcccactgta                                                                                                                                                                                                                                 >  2:436385/1‑232 (MQ=255)
        cgccgcTCTGTACCAGA‑TGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCCCCATATTGACg                                                                                                                                                                                                                               >  2:361029/1‑239 (MQ=255)
                       gA‑TGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGCGCTCTC‑GT‑CCGCCAGTGGACCCCGCAGGCCGGAGGCATAAATGCGGGTAACCCCGCCCTGGCGCATG‑TGG‑TCTACCAGCGGCGCATGTAACTCACTCAGGGGGCCGGTGGACAGGGGACGGGTTTTAAAGCCATGCTTCCCT‑CTGCGAGCCACCACCAAATTACCGGCCAGGTCACCGcc                                                                                                                                                                                                                 >  1:1286832/1‑238 (MQ=255)
                       gA‑TGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGGATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGGCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCAAATTAACGGTCAGGTCACCGcc                                                                                                                                                                                                                 >  2:1237791/1‑238 (MQ=255)
                       gA‑TGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGcc                                                                                                                                                                                                                 >  2:804317/1‑238 (MQ=255)
                       gA‑TGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGGCAGGTTTTTAAAGCCCATCTTCGCG‑CAGACGTCCATCACCataccgagctcttatacacaactg                                                                                                                                                                                                                >  1:135308/1‑218 (MQ=255)
                       gA‑TGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑AGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATAACGTCCATCACCATATTGCCGTTCAGGTCACCGcc                                                                                                                                                                                                                 >  2:308130/1‑238 (MQ=255)
                        a‑TGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGGCAGGTCACCGCCAc                                                                                                                                                                                                               >  2:19041/1‑239 (MQ=255)
                           gCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCCCgg                                                                                                                                                                                                             >  2:6970/1‑239 (MQ=255)
                             acacGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCCGGGAGCTGGACGCCCGGGGCAGGTTTTTAAAGCCCCTCTTCGCG‑ATGACGGCCACACCCATATTGACGGTCAGGACACCCCCCAGGaa                                                                                                                                                                                                           >  1:589614/1‑239 (MQ=255)
                             acacGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCCTCACCATATTGACGGTCAGGTCACCGCCACGGaa                                                                                                                                                                                                           >  2:1190404/1‑239 (MQ=255)
                             acacGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCCCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGaa                                                                                                                                                                                                           >  2:935286/1‑239 (MQ=255)
                             acacGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGCAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTAACCGCCACGGaa                                                                                                                                                                                                           >  2:760503/1‑239 (MQ=255)
                                    cgccgTGAAGGTGGACCTGCACCGGTTCTGCAAGCAGACCACGGGAAATCTT‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGa                                                                                                                                                                                                    <  2:1066694/239‑1 (MQ=255)
                                             gttgtttcgcccctgttggtGCCAGCAGACGCGGGGGGTTCTC‑GT‑CCGCAAGGGGACCACGCGGGCGGGAGGTATAAATGGGGGTAACCGCGCCCTGGCAAAGG‑TGT‑GCGCCCGGCGGCGAAGGGAAATCCCCACGGGAGCGGGACGCCGGGGTCGGGTTTTTAAACCCCACCTTCGCG‑ATGGCGTCCATCACCATATGGCCGGGAGGGTCACCGCCACGGAAAGCGTGATggaagga                                                                                                                                                                                            <  1:20556/219‑1 (MQ=255)
                                                 gATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATGACTCAGGGAGCTGGACGCCAGGGGCGGGGTTTGAAAGCCCATCTTCGCG‑ATGACGGCCATCACCAGATTGTGGGGCATGTACCCGCCACGGAAAACGGGATGGAAAGAAAACg                                                                                                                                                                                       >  1:1111724/1‑239 (MQ=255)
                                                 gATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCACGGCACTCACTCAGGGCGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCCTCGCG‑ATGACGTCCATCACCCTATTGACGGTAAGGTCACCGCCCCGGAAAGCGGGAAGGAAGGAAACCg                                                                                                                                                                                       >  1:864468/1‑239 (MQ=255)
                                                  attggccccGGTTCTGCAGGAAGCCCACGGGAGATCCC‑TT‑CCGCCAGGGGACCACGAAGGCCGGAGGTATAAAGGCGGGTAACCAGCCCCTGGCGAATG‑TGT‑TCTACCAGGGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCg                                                                                                                                                                                       <  2:48691/231‑1 (MQ=255)
                                                      gCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACCCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGGCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGTTGGAAGGAAACGGCCAt                                                                                                                                                                                   >  1:335953/1‑238 (MQ=255)
                                                       cACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAACTGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCCGGGAGCTGGGCTCCAGGGCCAGGTTTTTAACGCCCACTTTCGCC‑ATGCCGTCCCACACCCTATTAGCGGCAAGGCCCCCCCCACGAGAAGCGGACGGGAAGAAAAccaccagcc                                                                                                                                                                                 >  1:26303/1‑232 (MQ=255)
                                                       cACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATg                                                                                                                                                                                  >  2:1154482/1‑238 (MQ=255)
                                                         ccGGTTCTCCAAGCAGACCAGGGGAGATTGC‑CC‑CCGCCAGGGGACCCGGCGGGCCGGAGGTAAAAATGCGGGTAACCCCGCCCTGGCGAATG‑TGT‑TCACCCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATAACCATATGGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCg                                                                                                                                                                               <  2:315085/239‑1 (MQ=255)
                                                            gTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAACTGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGACGCTGGAGGCCCGGGTCAGGTTTTTAAAGCCCACCTTCCCG‑CTCACTTCTATCACAAACTTTACTGTCAGGCTACCGCCACGGAAAGCGTGATGTAAGCAAAccctacagccacc                                                                                                                                                                             >  1:239272/1‑227 (MQ=255)
                                                            gTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCGTCACCATATTGACTGTCAGGTCACCCCCACGGAAAGCGGGAAGGAAGGaaagagacataccgacc                                                                                                                                                                            >  1:390536/1‑225 (MQ=255)
                                                            gTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTcc                                                                                                                                                                            >  2:156356/1‑239 (MQ=255)
                                                            gTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAACCGTACTGGAAGGAAACCGTCATGcgtc                                                                                                                                                                              >  2:94203/1‑234 (MQ=255)
                                                             ttCTGCCACCATCCCACGGGCGATCTC‑GT‑CCGCCAGTGGACCACGCAGGACGGAGGTATACATGCGGGCAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGAACGCTAATCACTCAGAGCGCCGCGCGAGCGGGCCAGGTTTTTAAACGCCCTCCTCCGG‑TTCACGTCGATCACCATCATGACGGTGCGGACATCGCCACGGACCGCAGGATTGACGGAGACCGTCagcacgaccc                                                                                                                                                                           >  1:1298635/1‑230 (MQ=255)
                                                                tgccccagcagaaCGGGAGAGCTCATTCCCCCCAGGTGACCACGCAGGCCAGGGGTATAAATGGGGGAACCCACCCCGTTGCGAATG‑TGT‑TCTACCAGCGGCCAATGGCAATCCCCCGGGTGGCCGGACGCCAGGGTCAGGTTTTAAAAGCCCATCTT‑GCGAATGACGTCCATCACCATATTGCCGGCAAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTg                                                                                                                                                                          <  1:1154482/227‑1 (MQ=255)
                                                                 gCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTaa                                                                                                                                                                       >  2:1190380/1‑239 (MQ=255)
                                                                      cagACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑CCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTTAACCag                                                                                                                                                                  >  2:1028066/1‑239 (MQ=255)
                                                                       agACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCCCGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCaga                                                                                                                                                                 >  2:306863/1‑239 (MQ=255)
                                                                          ccACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAAGGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCCCCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCagag                                                                                                                                                                <  2:720667/237‑1 (MQ=255)
                                                                           aaCGGGAGTTCTC‑TT‑CCCCAAGTGGACCAGGAAGGCCGGTGTTATAAATCGCGGTAACCCCGCCCGGGCGAATG‑TGT‑TCTACCAGGGGCGCATGGCAATCACTCAGGGAGCTGGCCGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGcg                                                                                                                                                              <  2:764523/237‑1 (MQ=255)
                                                                              gggAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGGTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCCCCATATTGACGGTCAGGTCACCGCCACGGAAAGCGGGATGGGAGGCAACCGTCAGGCCGTCCTGCAACCCAAAGcgacgc                                                                                                                                                          >  1:875179/1‑238 (MQ=255)
                                                                                 gaaatcttct‑CCGCCAGTGGCCCACGCAGGCCGGAGGTATAAATGGGGGTAACCCCGCCCGGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACCCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGaa                                                                                                                                                         <  2:875179/229‑1 (MQ=255)
                                                                                   ttctc‑tc‑CCGCGAGGGGCCCAGCCTGGCCGGAGGTAAAAAGGGGGGACACCCCGCCCTGGGTAAGGTTGT‑TC‑GCCGGGGGCGCATGGAAAGCACGGAGGGAGGCGGACGCCAGGGGCTGGTTGTTAAAGCCAACCTTCGCG‑AGGACGTCCATCACCATATGGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCg                                                                                                                                                      <  1:967090/237‑1 (MQ=255)
                                                                                   tcttc‑tT‑CCCCCGGTGGACCCCGGTGGCGGGGGGTAAAAAGGGGGGTAACCCCGCCCTGGCGAATG‑TGT‑TCTACCGGGGGCGAATGGCAATCACTCGGGGAGTTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCg                                                                                                                                                      <  1:1190404/235‑1 (MQ=255)
                                                                                   aTCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCCTATTGACGGTCAGGGCACCGCCCCGGAAAGCGTGATGGAAGGAACCCTTCATGCCGTCCTGTAAACCAGTGCGCCGAATc                                                                                                                                                       >  2:166624/1‑237 (MQ=255)
                                                                                     ctc‑tT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑TTGACGTCCATCACCATATTGACGGTCAGGTCACTGCCCCGGAAAGCGTGATGGAAGGCAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGctt                                                                                                                                                   >  2:46450/1‑239 (MQ=255)
                                                                                     cccttt‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAAGGCGGGTAACCACGCCCTGGCAAATG‑TGT‑TCTACCAGGGGCCCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGct                                                                                                                                                    <  2:14535/235‑1 (MQ=255)
                                                                                            ccGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGGTTTTAAAGCCCATCTTCGCG‑ATAACGTCCATCCCCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAAACGTCATGCCGTCCTGAAAACCAGAGCGACGAATAGCTTCTTcc                                                                                                                                              >  1:760936/1‑239 (MQ=255)
                                                                                                       cccgcggggcatgAGGTGAAAAGGCGGTTAACCCCCCCCGGGCAAAGG‑TGT‑TCTCCCGGCGGCCTATGCAAACCCCAGGGGGGGCGGGACCCCAGGGTATGGTTTTAAAGGCCAATCTTCGCG‑CTGACGCCCACCCCCATATGGACGGTCAGGTAACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                                                                                                   <  1:312214/227‑1 (MQ=255)
                                                                                                       cccgccAGGCCGGAAGTATAAAGGCGGGTAACCACGCCCGGCCGAATG‑TGT‑TCTACCAGGGGCGCATGGCAAGCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCAATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                                                                                                   <  2:120471/234‑1 (MQ=255)
                                                                                                       ccccgcgGGCCGGGTGTATAAGTGCGGGTACCCCCGCTTTGGCGAATG‑TTT‑TCTACCAGGGGCGTAGGGCAATCACTCGGGGAGCTGGCCGCCGGGGTCAGGTTTTTAAAGCCACTCTTCGGG‑ATGACGTCCATCCCCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                                                                                                   <  1:156356/236‑1 (MQ=255)
                                                                                                       ccagccggggcGGGGGTGTAAAGGGGGGTAACCCCGCCGT‑GTGGATG‑GGTGTCTGCTGGCGGCTCAGGGCAATCACTCGGGGAGCTGGACGCCGGGGTCAGGTTTTAAAAGCCAATCTTCGGG‑ATGACGTCCATCGCCTTATGGCCGGTCGGGTCACCGCCACGGAAAGCGTGATGGAAGGAAAGCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTGCCAGGGGGGCGca                                                                                                                                   <  1:577199/229‑1 (MQ=255)
                                                                                                       ccAGGCAGGCCGGAGGTATAAATGGGGGTAACCGCGCCCTGGGGAATG‑TGT‑TCTACCAGCGGCGTATGGCAATCCCTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                                                                                                   <  2:1173894/239‑1 (MQ=255)
                                                                                                       ccACGCAGGCCGGCGGAATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGCTTTTTACAGCCCATCTTCGCG‑CTGACGTCCATCACCAAATTGACTGCCAGGTCACAGCCACGGAAAGCGCGCTGCAAGGAAACCGTCATAACGCCCTGTCAACCAGCGCGACGTAACGACTCTCCCAGGGTGgcacc                                                                                                                                   >  1:781841/1‑236 (MQ=255)
                                                                                                        cccgcgGGCCGGAGGTATAAAGGCGGGTAACCACGCCCTGGCAAATG‑TGT‑TCTCCCAGCGGCGCATGGCAATCACTCAGGGAGCGGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCCCCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                                                                                                   <  2:1249783/236‑1 (MQ=255)
                                                                                                        cccGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCCCCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                                                                                                   <  2:434969/236‑1 (MQ=255)
                                                                                                        cACGGAGGCCGGAGGTATAAATGGGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                                                                                                   <  2:112244/238‑1 (MQ=255)
                                                                                                        cACGCGGGCCGGAGGTAAAAAGTCGGGTAACCGCGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTAAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCAATCTTCGGG‑ATGACGTCCATCCCCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                                                                                                   <  2:1278117/238‑1 (MQ=255)
                                                                                                          cGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCCGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCCCCCCGGCACGCGTGATCGCAGGAACACGGCCAGCCGTTCACTAAACCCAAGACACGCATCGCTTCTTCAAGGGTGGCCcacca                                                                                                                                >  1:181467/1‑237 (MQ=255)
                                                                                                          cGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGCAAACCACCGCGACGAATACACTCTTCCCGGTTgggccccca                                                                                                                                >  1:112244/1‑232 (MQ=255)
                                                                                                                     ggTAAAAATGGGGTTAACCCCGCCCTGGCAAATG‑TGT‑TCTACCCGCGGCGCATGGAAATCCCCCAGGGAGCTGGACGCCAGGGTAAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcg                                                                                                                      <  2:745780/238‑1 (MQ=255)
                                                                                                                         taAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTAAGGTCACCGCCACGCAAAGCGGGATGGACGGAAACCCTCCTTCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGAACACTTTGCAAgcgcgc                                                                                                                   >  1:298639/1‑237 (MQ=255)
                                                                                                                              gCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCtttt                                                                                                             >  2:211460/1‑238 (MQ=255)
                                                                                                                                 ggTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGCCGAATCGCTTCTTCCAGCTTGGCGCACAGTTTGGGATGGCGCGCTTTTTCa                                                                                                          >  2:200066/1‑238 (MQ=255)
                                                                                                                                 ggTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTTCGATCGCGCGCTTTTTCa                                                                                                          >  2:1316142/1‑238 (MQ=255)
                                                                                                                                 ggTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATCCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGCCGCACAGTTTGCGATCCCGCGCTTTTTCa                                                                                                          >  2:1170492/1‑238 (MQ=255)
                                                                                                                                           cccTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTC‑GGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGtt                                                                                              >  2:541825/1‑239 (MQ=255)
                                                                                                                                            ccTTGCGAATG‑TAT‑TCTCCCAGCGGCGCGAGACAATCACTCAGGGAGCTGGACGCCAGGGTCAGGATTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGAGGGAAGGAAACAGTCATGCCTGCCTGTAACCCACAGCGAGCAATCGCACCATCCAGTTTGGCTCACAGTTTGTGACCGCGCGCTTTTTCAGCCgggatggat                                                                                              >  1:1147935/1‑233 (MQ=255)
                                                                                                                                               ggCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGttt                                                                                             >  2:1212158/1‑237 (MQ=255)
                                                                                                                                                gCGAATG‑TTT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGc                                                                                                                                                     <  2:1089832/180‑1 (MQ=255)
                                                                                                                                                gCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGc                                                                                                                                                     >  1:1089832/1‑180 (MQ=255)
                                                                                                                                                gCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCt                                                                                          >  2:1161247/1‑239 (MQ=255)
                                                                                                                                                     tg‑tgT‑TCTACCAGGGGCGCATGGCAATCACCCAGGGAGCTGGCCGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCCCCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGc                                                                                     <  2:589614/239‑1 (MQ=255)
                                                                                                                                                      gttgt‑TCTTCCATCGGCGCGTGGCACTCTCTCGGGGGTCGGGACGCCGGGGTCGGTTTTTTAACGCCCACCCTCGCG‑ATGACGTCCACCACCATATTGCCGGCCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGc                                                                                     <  1:345026/237‑1 (MQ=255)
                                                                                                                                                            tCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGGTTGCTTTGCGGAGt                                                                                 >  2:298922/1‑238 (MQ=255)
                                                                                                                                                            tCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGCCGCCCAGTTTGCGATCGCTCGCCTTTTCAGCCTGGCAGGTTCGCTTTGGCGGGtt                                                                                >  2:1134679/1‑239 (MQ=255)
                                                                                                                                                             ctacccggggccctgggaaaccACTCAGGGAGCTGGCCGCCAGGGTCGGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTc                                                                               <  2:501759/218‑1 (MQ=255)
                                                                                                                                                                ccAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTgg                                                                            >  2:1075054/1‑239 (MQ=255)
                                                                                                                                                                   gcggcgCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATTCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGCTCCCGCGCTTTTTCAGCCTGTTATGTTTGCTTTGGCGAGTTCTGTaaa                                                                         >  2:1193835/1‑239 (MQ=255)
                                                                                                                                                                   gcggcgCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGaac                                                                         >  2:345026/1‑238 (MQ=255)
                                                                                                                                                                   gcggcgCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGaaa                                                                         >  2:636309/1‑239 (MQ=255)
                                                                                                                                                                         gatggccattgctcggtgtgcgggACGCCCGGGGTGGGTTTTTAAAGCCACGCCCCGCG‑AGGCCGCCCAGCCCCATGTTGGCGGTCAGGTCAGCGCCACGGAAAGCCTGATGGAAGGAAACCGTCATGCCGTCCTGGAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGGGAGTTGTGGAAAGCGGCa                                                                   <  1:760503/217‑1 (MQ=255)
                                                                                                                                                                         gatgccaacccttcggggAGCTGGCAGCCTGGGTTGTGTTTTTAAAGCCCCTCATCGCG‑GTGACGTCCACAACCATATTGTCGGGCAGTTCCCCGCAACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCa                                                                   <  1:1028066/224‑1 (MQ=255)
                                                                                                                                                                                 tccctaggggAGCTGGCCCCCAGGGTAAGGTTTTTAAACCCCATCTTCGGG‑AAAAGGCCCATCACCAATATGCCGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCa                                                           <  2:760936/232‑1 (MQ=255)
                                                                                                                                                                                 tCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACAAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGGGTTCTGGAAAGCGGCAAGATTGCa                                                           >  2:527386/1‑239 (MQ=255)
                                                                                                                                                                                       aGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGGAAGCGGCAAGATCGCATTCAgc                                                     >  2:331341/1‑239 (MQ=255)
                                                                                                                                                                                         ggAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGCTCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGGGGCAAGATGGCATTCAgccc                                                   >  2:284071/1‑237 (MQ=255)
                                                                                                                                                                                            cgtgtaCGCCAGGGTAAGTTTTTTAATGCCCATCTTCGCG‑ATGACCTCAAGCACCATATTGACGGCCGGTCCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGcga                                                 <  1:306863/233‑1 (MQ=255)
                                                                                                                                                                                             gtGGACGCCGGGGTCAGGTTTTAAAAGCCCATCTCCGCG‑ATGCCGTCCATCACCATATTGCCGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGcgac                                                <  2:26303/237‑1 (MQ=255)
                                                                                                                                                                                             cTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGcgacg                                               >  2:223834/1‑239 (MQ=255)
                                                                                                                                                                                                   gcaaGGGTACGGTTTTTAAAGCCCAGCTCCGCG‑ATGACGCCCATCACTAAATTGACGGTGAGGTCCCCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGGCCTGTAAACCAGAGCGACGAATCGCTTCGTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCa                                         <  2:781841/236‑1 (MQ=255)
                                                                                                                                                                                                   ccacgggTCGGGTTTTTAAACCCCATCCTGGGG‑ATGCCTCCCACCACCATATTGAGGGTCAGGTCACCGGCACGGAAAGCGTGATGGAAGGAAACGGTTATGCCGGCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCa                                         <  1:1190380/235‑1 (MQ=255)
                                                                                                                                                                                                     cAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGACTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTACGCGCGACGATTCCAg                                        >  2:1020936/1‑238 (MQ=255)
                                                                                                                                                                                                                   tAAACCCCATTTTCCGG‑ATGCCGTCCACGCCCATTTTGAGGTGCAGGTCCCCGCCCCGGGAAGCGTGATGGAAGGAAACCGGCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGATTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGTTCt                         <  1:622754/239‑1 (MQ=255)
                                                                                                                                                                                                                        cccATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGTTCTTTTCg                    >  2:501930/1‑239 (MQ=255)
                                                                                                                                                                                                                           aTCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGCGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGTTCTTGTCGTTg                 >  2:1189408/1‑239 (MQ=255)
                                                                                                                                                                                                                            tCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTCGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAAGTCGCATTCAGCGCGCCGTTTCCAGGCCGCGACCCGTTTTTGTCGTTga                >  1:735270/1‑239 (MQ=255)
                                                                                                                                                                                                                            tCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGTTCTTGTCGTTga                >  2:482647/1‑239 (MQ=255)
                                                                                                                                                                                                                            tCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCCAGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAGATCCCATTCGACGCGACGATTCCAGGCCGCTACCCGTCCTTTTCGTTga                >  1:1206232/1‑239 (MQ=255)
                                                                                                                                                                                                                                cgcg‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGTTCTTTTCGTTGGGAtt            >  2:593420/1‑239 (MQ=255)
                                                                                                                                                                                                                                        aCGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGCAGGTTTGCTTTGGCGGGTTCTGGAAAGCGGCCATGTCGCATTCACCGCGCCGTTTCACGGCCGCACCCCGTCCtgttgtttgcatgttttcaat     >  1:444991/1‑219 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                        
ATTCAGTTCGCCGCTCTGTACCAGA‑TGCACACGACCGCCGTGAGAGTGGATCTGCACCGGTTCTGCCAGCAGACCACGGGAGATCTC‑TT‑CCGCCAGTGGACCACGCAGGCCGGAGGTATAAATGCGGGTAACCACGCCCTGGCGAATG‑TGT‑TCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCG‑ATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCAAGATCGCATTCAGCGCGACGATTCCAGGCCGCTACCCGTTCTTGTCGTTGAGATTGTTCAATTTT  >  NC_000913/649095‑649562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A