Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F18 I0 R1 274 115.5 2655328 86.7% 2302169 226.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 861,108 A→C 16.7% V500G (GTG→GGG)  ybiW ← putative pyruvate formate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913861,1080AC16.7% 226.6 / 4.8 97V500G (GTG→GGG) ybiWputative pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base A (48/28);  new base C (7/13);  total (55/42)
Fisher's exact test for biased strand distribution p-value = 4.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCACTGCCGCCAGGCTGTTGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTGCGGTTCAATACCGAGTTTAAT  >  NC_000913/860876‑861337
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       
cttccccgcccgcaagttGTTGCCGGAGTTGGACATGCCGACCTGCAGGCCAAAAACCCAGTCAAATTGCGCGCCGCCTTGCTTGATTCTTTTCGCTCGCTCAATTCAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGAACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCAt                                                                                                                                                                                                                                   <  1:799703/222‑1 (MQ=255)
 ttcACTGCCGCCAGGCTGTTGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGCCA‑TATTCGAT‑TTCGATTGATTTGCGGGGGTAGTAACGGATTTCGGGATCCCACCCGTCCATCCCTTCCTc                                                                                                                                                                                                                                  >  1:353572/1‑239 (MQ=255)
  tcACTGCCGCCAGGCTGTTGCCGAGGTAGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTGGCGCCGCCTTGCTTTATACTTTTCGCTCGCTCAATACAGTCATCCACCACCGCCGAGCAGAGACTATCGTGCACGATCTCTTCCAGCAGGGGGTCGACGCCA‑TATTCGAT‑TTCGATTGATTTGCGGCGGTAGTAAAGGATTTGCGGATCCCACGCGTCCATCACTTCATCg                                                                                                                                                                                                                                 >  1:648057/1‑239 (MQ=255)
   ccctcccgccTGGTTGTTGCCGGTGTTGGCCATTCCATACTGCAGGTCAGAAACCCAGTCATATTGTGCGCCGCCTTACTGAAGTATTGTCGGTCGCGCAATTCAGTCATCCACCAGCGCGGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGGAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGa                                                                                                                                                                                                                                <  1:982493/234‑1 (MQ=255)
   cACTGCCGGCAGGCTGTTGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGa                                                                                                                                                                                                                                <  2:747111/239‑1 (MQ=255)
      tGCCGCCAGGCTGTTGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATTTCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAgt                                                                                                                                                                                                                             >  2:695346/1‑239 (MQ=255)
        ccgccAGGCTGTTGCCGAGGTTTGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGATCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAgttg                                                                                                                                                                                                                           <  2:1229926/239‑1 (MQ=255)
         cgccAGGCTGTTGCCGAGGTTGGCATTGCCGACCCTCACGCCAGAACCCCAGTTCTATTTCGCGCCGCCGTGCTTGATACTTTTCTCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAgttgt                                                                                                                                                                                                                          <  2:88960/239‑1 (MQ=255)
             aGGCTGTTGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGCCGACA‑TATTCGAT‑TTCGATTGCTTTGCGGGTGTAGGAACGGACTTGGGTTTCCCACTCGTCCATCATTTCACCGAGATTGTTaaa                                                                                                                                                                                                                      >  1:512476/1‑239 (MQ=255)
                  gTTGCCGAGGGTGGCAAGACCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCCCGTTGTCTTCCAGCATGGGGTCGCCGACA‑TATTCGCT‑TTGTATTGATTGGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCCCTTCagacgagtagttgagctaac                                                                                                                                                                                                                 >  1:910564/1‑220 (MQ=255)
                   ttGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGCT‑TTCGATTGATTTGCGGGAGGAGTAAAGGATTTGCGGATCCCACCCGGCCCTCCCTCCATGGAAATTGTGGAAGTTAcc                                                                                                                                                                                                                >  1:1069302/1‑239 (MQ=255)
                   ttGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTa                                                                                                                                                                                                                  >  2:533395/1‑237 (MQ=255)
                   ttGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGGAACGGATTTGCGAATCCCACGCTTCCATCCCTTCTTCGAAGTTGTTGAAGTTa                                                                                                                                                                                                                  >  2:955922/1‑237 (MQ=255)
                             tGGCCATGCCCCCCTGCAGGCCAAAAACCCAGTCATTTTTCGCCCCGCCTTGCTTGATTCTTTTCGCCCGCTCAATACAGTCCTCCACCAGCGCCGAGCAGAGATTTTCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTAGCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAAc                                                                                                                                                                                                      <  1:909429/239‑1 (MQ=255)
                             tGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGCCGACA‑TATTCGAT‑TTCGATTGATTTGCGGGGGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCACAGTTTTTGAAGTTACCTGCCgcaaa                                                                                                                                                                                                       >  1:313419/1‑234 (MQ=255)
                                  aTGCCTCCCTGTAGCCCAGAAACCCAGTCATTTCTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCGCAATACAGTCATCCACGTGCGCCGAGCATAGAATATCGTGCCCGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAATTTACCTGCCGACAACGCtt                                                                                                                                                                                                  <  1:592269/238‑1 (MQ=255)
                                   tGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTGGTAAAGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAGGTGGTTTAAGTTAACTGCCGCCAAGGCtttt                                                                                                                                                                                                >  2:614933/1‑239 (MQ=255)
                                   tGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCAACGCGTCCATCCCTTCATCGAAGTTGTTTAAGTTACCTGCCGCAAACGCtttt                                                                                                                                                                                                >  2:412197/1‑239 (MQ=255)
                                     ccGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCtttt                                                                                                                                                                                                >  2:101333/1‑237 (MQ=255)
                                        aCCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTtg                                                                                                                                                                                           >  2:499547/1‑239 (MQ=255)
                                        aCCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTCTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGCAGTAACGGACTTGCGTATCCCACGCGTCCATCCCTTCTTCGAGGTTGTTTAAGTTCCTTGCCGACAAACCtttttttt                                                                                                                                                                                            >  1:1284871/1‑238 (MQ=255)
                                         ccTGCAGGCCAGAACCCCAGTCATATTTCGCGCCGCCTTGCTTGATTCTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTtgt                                                                                                                                                                                          <  2:691772/239‑1 (MQ=255)
                                         ccTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGGGTAGTAACGGATTTGCGAATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCACCAACGCTTTTTCTtgt                                                                                                                                                                                          >  2:727682/1‑239 (MQ=255)
                                          cggCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTtgt                                                                                                                                                                                          <  2:353572/236‑1 (MQ=255)
                                           ttctggCTAGAACCCCAGTCTTTTTTCGCGCCGCGTGGCTGGATTCTTTCCGCTCTCTCAATACCGGCATCCCCCGGGGCCGAGCAGGGAATATCGTGCACGTTCTCTTCCAGCATGGGGGCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTgg                                                                                                                                                                                        <  1:70480/235‑1 (MQ=255)
                                                     aaaCCCCGCCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCCCCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGCCA‑TATTCGCT‑TTCGATTGATTTGCGGGGTTCGTAACGGATTTGCGTATCCCACGCGTCCATCCCTTCATCGAAGTGTTTGAAGTTACTTGCCGACAACGCTTTTTCTTGGGGCAG‑Gaaacct                                                                                                                                                                             >  1:810488/1‑235 (MQ=255)
                                                          cAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGCTTTGCGTATCCCACGCGTCCATCACTTCATCGACGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGTCG‑GAAACCttttc                                                                                                                                                                         >  2:190286/1‑236 (MQ=255)
                                                            gTCATGTTTCGCGCCGCCTTGCTTGATTCTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGc                                                                                                                                                                      <  2:1156654/239‑1 (MQ=255)
                                                            gTCATATTTCGCGCCGCCTTGCTTGATTCTTTTCGCCCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGc                                                                                                                                                                      <  2:905965/239‑1 (MQ=255)
                                                                  tttCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACAATTTGCCGCt‑ggtg                                                                                                                                                                >  2:176844/1‑238 (MQ=255)
                                                                  tttCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTCCCGACAACGCTTTTTCTTTGGGCAG‑GGAAACTTTTCCGCc‑ggtgg                                                                                                                                                               >  1:820784/1‑239 (MQ=255)
                                                                      gggccgccTTGCTTTATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCTCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGcatc                                                                                                                                                           <  2:461174/237‑1 (MQ=255)
                                                                       cgccgcCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCCTGGGGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCCTCACTTCATCGAGCTTGTTCAAGTTACCTGCCGACAACCCTTTTTCTTGTGGCAG‑GAAAATCTTCCCGCC‑GGGGGcatca                                                                                                                                                          >  1:1214957/1‑239 (MQ=255)
                                                                       cgccgcCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGcatca                                                                                                                                                          >  2:1288371/1‑239 (MQ=255)
                                                                       cgccgcCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAAAACTTTTCGGCT‑GGTGGcatc                                                                                                                                                           >  2:496600/1‑238 (MQ=255)
                                                                       cgccgcCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTCCCGACAACCCTTTTCCTTGGGGCAg‑gtaacctatgcctcgtctggacac                                                                                                                                                            >  1:461174/1‑215 (MQ=255)
                                                                           gccTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGGGGCATCATGc                                                                                                                                                       >  2:1177339/1‑238 (MQ=255)
                                                                           gccTTGCTTGATACTTTTCCCTCGCTCAATGCAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCTCGCGTCCATCCCTTCATCGAAGTTTTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGcc                                                                                                                                                      <  2:182730/239‑1 (MQ=255)
                                                                            ccTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGCGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGCGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGCCAACGCTTTTTCTTGTGGCAG‑GAACACCTTCCCCCC‑GGTTCCATCCTGcc                                                                                                                                                      >  2:987019/1‑238 (MQ=255)
                                                                             cttgcttgATTCCTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTTTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCg                                                                                                                                                    <  2:1081527/239‑1 (MQ=255)
                                                                                      tttttgtcgcttgtTCAATGCAGTGCGCCCCCAGCGCCGAGCAAAGGATATCGTGCACGTTCTCTTCCAGCGTGGTGTCGCGGACA‑TATTCGAT‑TTCGTTTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTCACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACGCTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAgc                                                                                                                                           <  1:496600/233‑1 (MQ=255)
                                                                                      tACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTc                                                                                                                                                                                              >  2:1111424/1‑190 (MQ=255)
                                                                                           ttCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTTTGGCAG‑GAACACTTTTCCGCT‑GGTGGCATCATGCCCGCCTTCCCGcgccg                                                                                                                                       >  2:1200047/1‑238 (MQ=255)
                                                                                           ttCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGCATCCCACGCGTCCATCACTTCATCGCAGTTGTTGAAGTTACCTGCCGTCAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCACCC‑GGTGGCATACTGCCCGCCTTCCAccgccg                                                                                                                                       >  2:97629/1‑238 (MQ=255)
                                                                                           ttCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGCGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTATTGAAGTTACCTCCCGACAACGCTTTTTCTTTTGGCAG‑GACAACTTTTCCGCTCGGGGGCA‑CAAGCCCCCCTTACAgccgcca                                                                                                                                      >  1:23131/1‑234 (MQ=255)
                                                                                           ttCGCCCGCTAAAGACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGcgccgc                                                                                                                                      <  2:1069302/239‑1 (MQ=255)
                                                                                                tcgctcAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGCCAACGCTTTTTCTTGTGGCAG‑GAAACCCTTGCCGCT‑GGTGGCATCCTGCCCCCCTTCCAGCGCCCCCacc                                                                                                                                  >  2:315745/1‑236 (MQ=255)
                                                                                                   ctcAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATTCCCGCCTTCCAACGCCGCCAGCATc                                                                                                                               >  2:951211/1‑238 (MQ=255)
                                                                                                     caattacgTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCAcg                                                                                                                            <  2:199922/232‑1 (MQ=255)
                                                                                                             tCACCCCCCGGCGCCGGGCTGAATATCTTGTCCCCGTTTTTTTCCATCTTGGTGTGGATGACA‑GACTCAAT‑TTCGATTGATTTGCGGGTGAAGTAACTGATTTGCGTACCCCACCCTTCCATCCCTTCATCGAAGTTTTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGaa                                                                                                                    <  1:1200047/239‑1 (MQ=255)
                                                                                                              cagccaccaGCGCCGAGCATAGAATTTCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCAGCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGc                                                                                                                       <  2:931160/232‑1 (MQ=255)
                                                                                                              cATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTTCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACCCGGGCGAAg                                                                                                                   >  2:180289/1‑239 (MQ=255)
                                                                                                                  caccaGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTACCGGATTTGCGTATCCCACGCGTCCATCACTTCATCGACGTTGTTGAAGTTACCTGCCGCCAACGCTTTTTCTTGTGGCAT‑GAACACTTTCACCCT‑GGTGGCCCAACTCCCCCCTTCCCGCGCCCCCCGCATCACCCGGGCggcgctgt                                                                                                               >  1:992694/1‑232 (MQ=255)
                                                                                                                    ccgaccgcgggCAGAGAATTTCGGTCGCGTTTTTTTCCAGCAGGGTGTCGGCGACA‑TTGTCGAT‑TTCGATTGATGTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATa                                                                                                             <  2:810488/229‑1 (MQ=255)
                                                                                                                       gcgcCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATTACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTTCCGCT‑GGTGGCATCATTCCCGCCTTCCAGCGCCGCCACCATCACCCGGGCGAAGTTGTTaaa                                                                                                           >  2:1243971/1‑238 (MQ=255)
                                                                                                                       gcgcCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTTCCGCC‑GGTGGCATAATGCCCGCCTTCCAGCGCCGCCAGCATCACGCCGGCGCAGTTGGTaaa                                                                                                           >  2:965860/1‑238 (MQ=255)
                                                                                                                       gcgcCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCCCCTTCCACGGCCGCCCGCCTCACGCGGGGGGAGCTGGTAAAg                                                                                                          >  2:154065/1‑239 (MQ=255)
                                                                                                                             aGCAGAGAATATCGTTCACGTTCTCTTCCAGCAGGGTGGCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGc                                                                                                                       <  2:8478/220‑1 (MQ=255)
                                                                                                                             aGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTACCGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGCCAACGCTTTTTCTTTTGGCAGCGAAAAC‑TTGCCGCT‑GGTGGCAGCAGTACCGCCTTCCTCCCCCCCCCCACTCACCCGGGCGAGCCTGATAAAGCCCac                                                                                                     >  1:1242469/1‑237 (MQ=255)
                                                                                                                             aGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCCCT‑GGTGGCATCAAGCCCGCCTTCCAGCGCCCCCCGCATCCCCCGGGCCAAGTTGTTAAAgtccat                                                                                                     >  2:177614/1‑233 (MQ=255)
                                                                                                                             aGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAAAACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGCAGTTGATAAAGCCCAt                                                                                                     >  2:1002649/1‑238 (MQ=255)
                                                                                                                               cAGTGAATTTCGTGCCCGTTTTCTTCAGGCGTGGGTACGAGAACA‑TTTTCTGT‑TTCGATTGATTTGGGGGTGTAGTAACGGATTTGCGTTCCCAACGCGTCCATCCCTTCATCGAAGTTGTTGAATTTCCCTGCCGACACCGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGc                                                                                                   <  1:176844/238‑1 (MQ=255)
                                                                                                                                   ttcttttgttctttttttctTCCAGCGTGGTGTCG‑CTTCATTTTTTGAT‑GTCGATTGATTTGCGGGTGTAGTAACGTAGTTGCGTGTCCCACGCGTCCATCACTTCATCGAGGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAT‑GAACACTTTGCCGCG‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTa                                                                                              <  1:499547/222‑1 (MQ=255)
                                                                                                                                    aaTATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GGAAACTTTTCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTTGTAAACATCCTGCCGGtgc                                                                                             >  2:206458/1‑237 (MQ=255)
                                                                                                                                     atttCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTAc                                                                                             <  2:1141006/235‑1 (MQ=255)
                                                                                                                                     atatGGTGCACGTTCTCTTCCAGCATGGTGTCGACACCA‑TTTTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTTTCCCACGCTTCCATCCCTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACa                                                                                            <  2:308006/239‑1 (MQ=255)
                                                                                                                                            gCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCCGCATCACGCGGGCGAAGTTGATAAAACTCCTGCCGGTCCACCGCTa                                                                                      >  2:775148/1‑238 (MQ=255)
                                                                                                                                            gCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGGGGCATCATTCCCGCCTTCCAGCGCCGCCAGCCTCACCCGGGGGAAGATGTTAAAGCCCATTCCGGTACAGCGaaa                                                                                      >  2:745601/1‑236 (MQ=255)
                                                                                                                                                      ttCCAGAATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCt                                                                                                        <  2:774974/210‑1 (MQ=255)
                                                                                                                                                            cATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACCCTTTTCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACCCGGGGGAAGTTGATAAAGCTCAATCCGGTACAGCGATAGCCCCCTTTGCAACCg                                                                      >  2:1241528/1‑238 (MQ=255)
                                                                                                                                                            cATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAAAACTTTTCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACCCGGGCGAAGTTGGTAAAGGTCATTCCGGTCAAGCGCAAGCCCCCTTTTCCAcccc                                                                     >  2:758758/1‑237 (MQ=255)
                                                                                                                                                                gtgtCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTTTGGCAG‑GGACCAGTTTCGGCT‑GGTGGTCTCATGCTCCCCTTTCACGGCCGCCACGCTGCCCCGGGCGAAGTTTGAAAAACTAAATCCGGTCCACCGAAAACCCCATCTGCCAcacaacgg                                                                 >  1:298749/1‑232 (MQ=255)
                                                                                                                                                                   tCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTACCGGATTTGCGTATCCCACGCTTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACCCTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGt                                                              <  2:46018/239‑1 (MQ=255)
                                                                                                                                                                    ttctatcatttttgagt‑ttctattTGTTTGCGAGTGTCGTTACGTATTTTCTTCTCCTACGCGTCCATGTCGTCATCGAATGTTTTGAATTTCCCTGCCTACAACTCTTTTTCTTGGGACAG‑GAACTCTTTGCCGCG‑GGTGGCCTCACGCCCGCCTTCCGGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATATCCCCATTTGCCACCGACGGCGGt                                                              <  1:466835/223‑1 (MQ=255)
                                                                                                                                                                    ggactcta‑tatTTGATGTTTGATTTTTTTGCGGGTGATGTATCGGGTTGGCGTCACCCACGTGTCCGGCCATTCATCGAAGTTGGTGAACTTGGCTCCTCACAACGCTGTTTCTTGTGGCAG‑GAACTCTTTGACGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGt                                                              <  1:534103/232‑1 (MQ=255)
                                                                                                                                                                    cgacgaCA‑TATTGGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGtt                                                             <  2:1284871/239‑1 (MQ=255)
                                                                                                                                                                          cgatttctctttttctgtttattTGGGGGTGTAGTAACGGATTTGCGTCTCCCTGGCGTCATACCCTTCAGCTAGTTTGTGTAAGTTCCCTGCCGACAACGCTTTTTCTGGTGGCAG‑GAACACTTTGCCGCG‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCta                                                         <  1:177614/219‑1 (MQ=255)
                                                                                                                                                                           a‑tatTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑TAACACTTTGCCGCT‑GGAGGCAGCTAGCCCCCCCTCCAGCCCCCCCCGCTCCCCCCGGGGCAGGTTTGGAAAGCCACTGCCGCTACAACGATAGCCCCCATTGACCCCCAcggcggggttaaaaa                                                      >  1:646616/1‑230 (MQ=255)
                                                                                                                                                                                        cgattGTTTTGGGGGTGTAGTACCGGATTTGCGTATCCCACGCGTCCATCCTTTCATCGAAGTTGTTGAAGTTACCGGCCGACAACGCTTTTTCTTGTGGCAG‑GACCACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCt                                           <  2:69400/239‑1 (MQ=255)
                                                                                                                                                                                          gttgattTGCGGGTGTAGTAAGGGATTTGCGTATCCCCCCCTTCCATCCCTTCATCGAATTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGc                                         <  2:992694/238‑1 (MQ=255)
                                                                                                                                                                                          gttgattTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCCCTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGc                                         <  2:294850/238‑1 (MQ=255)
                                                                                                                                                                                          attgattTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCCCCTTCCAGCGCCGCCAGCATCACGCGGGCCAAGTTGTTAAAGCTCATGCCGGTACAGCGATAGCCCCATTTTCCACCGCCGGCGGGTTTTTTCCAACCCATCgacgc                                         >  2:55481/1‑235 (MQ=255)
                                                                                                                                                                                           ttggttTGGGGGTGTAGTAACGGATTTCGGTATCCCAAGGGTCCATCCTTTCATCGAAGTTGTTGAAGTTCCCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACCCTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCg                                        <  2:586121/239‑1 (MQ=255)
                                                                                                                                                                                           ttgattTGCGGGGGTAGTAACGGATTTGCGTCTCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGAAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGc                                         <  2:313419/238‑1 (MQ=255)
                                                                                                                                                                                            tggtttGGGGGTGTTGTAACGGATTTGCGTATCCCACGGGTCCATCACGTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGt                                       <  2:893125/236‑1 (MQ=255)
                                                                                                                                                                                                        gtagtaACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGGTAGCCCCATTTGCCACCGACGGCGGGTTTTATACAACCAATCGCTGCGTAGTCATAAGCg                            >  2:394319/1‑238 (MQ=255)
                                                                                                                                                                                                            tacctgttttGCTTCTCCCACCCGCTCCTCCCTTAATGGGAGTTTTTGAAGTTGCCGGCCGACACCGCTTTTTCTTGGGGCAG‑GAACCTTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTg                       <  1:180289/232‑1 (MQ=255)
                                                                                                                                                                                                            taACGGATTTGCGTATCCCACGCGTCCATCCCTTTATCGAAGTTGTTGAAGTTACCTGCCGACAACGTTTTTTCTTGGGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTg                       <  2:648057/239‑1 (MQ=255)
                                                                                                                                                                                                              aCGGATTTGCGTTTCCCACGCGTCCATCCTTTCATCGAAGTTGTTGAAGTTCCCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTGCg                     <  2:1253869/239‑1 (MQ=255)
                                                                                                                                                                                                               gatgggtaaaagagacaggcgTCCATAACTTCATCGAAGTTTGTGAAGTTCCCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTGGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTGCg                     <  2:1267935/220‑1 (MQ=255)
                                                                                                                                                                                                                 gATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTTCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCCTCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCCTTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGGCCTAAACGTCCTGCGGtt                  >  2:247395/1‑239 (MQ=255)
                                                                                                                                                                                                                   tttGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCCTTTGCCACCGACGGCGGTTTCTATACAACCAATCCCCGCGTAGCCATAAACGTCCTGCGGTTCa                >  2:481339/1‑239 (MQ=255)
                                                                                                                                                                                                                    ttGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCCCCGACGGCGGGTTTTTTACAACCAAACCCTGCGTAGGCATAAACGTCCTGCGGTTCaa               >  2:901673/1‑239 (MQ=255)
                                                                                                                                                                                                                     tGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAACTCATGCCGGTACAGCGATAACCCCAATTGCCACCGACGGCGGGTTCTTTTCAACCAATCGCTCCGACGTCATAAAGGTCCTCCGGTTCata              >  2:641298/1‑237 (MQ=255)
                                                                                                                                                                                                                      gCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGGTTCTTTACAACCAATCGCTGCGTAGTCATAAACGGCCTGCGGTTCAAt              >  2:358875/1‑238 (MQ=255)
                                                                                                                                                                                                                            cccACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCCGCGCCGCCAGCATCACGCGGGCGAAGTTGTTAAAACTCCTTCCGGTCACGCGGTCACCCCATTTGCCACCGACGGCGGGTTCCATATCACCACACGCCGCGAAGACCCAAACGCCCTCCGGTTGAAAAccacgt       >  1:1272587/1‑235 (MQ=255)
                                                                                                                                                                                                                                 gcgTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGGGGCCTCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGACGTTGATAAAACTCATGCCCGGACCGCGATAACCCCATTTGCCCCCGGCCGGGGGTTCTTTTCAACCAAACGGCGCCGAGTCAAACACGTCCGCCGGGTCCATAACGAgggtaaa  >  1:248747/1‑233 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       
CTTCACTGCCGCCAGGCTGTTGCCGAGGTTGGCAATGCCGACCTGCAGGCCAGAAACCCAGTCATATTTCGCGCCGCCTTGCTTGATACTTTTCGCTCGCTCAATACAGTCATCCACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACA‑TATTCGAT‑TTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAG‑GAACACTTTGCCGCT‑GGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTGCGGTTCAATACCGAGTTTAAT  >  NC_000913/860876‑861337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A