Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F18 I0 R1	274	115.5	2655328	86.7%	2302169	226.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,280,456	C→T	12.7%	L160L (CTG→TTG)	yjcE →	putative cation/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,280,456	0	C	T	12.7%	203.0 / 2.4	93	L160L (CTG→TTG)	yjcE	putative cation/proton antiporter
Reads supporting (aligned to +/- strand): ref base C (36/42); new base T (3/10); total (39/54)
Fisher's exact test for biased strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAA  >  NC_000913/4280218‑4280689

actttgtgtgggtaTTTGCGGCCTCGCGCTGGGGTTGGTGGTGGTGCCCGTGGTGGGCATTGTCTTTCTTATTTAGTGGGTGGTGGCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGGGCTTTCGCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTc                                                                                                                                                                                                                                                  <  1:413768/226‑1 (MQ=255)

                ttCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTTCAGGGCGCAGCGTTGGTG‑AATGACCCCTCCGGTCTGGTGTCCTTTGAGtt                                                                                                                                                                                                                                  >  2:1244764/1‑239 (MQ=255)

                ttCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGGGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGCTG‑AATGACGCCTCCGGTCTGGGGTCGTTGAAggt                                                                                                                                                                                                                                  >  2:784664/1‑237 (MQ=255)

                        cgcgCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGAAGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAg                                                                                                                                                                                                                          >  2:909944/1‑239 (MQ=255)

                             gggcggtggTGTTGGTTCCCGGGGGTGGCTTTGGTTTCCTTATTTTCTGGGGGGGGCGTGTCATTGCGGTGATCCCTGCCTTTGCGCGGGGGGCGGTGCTTTCGCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGt                                                                                                                                                                                                                     <  1:130920/237‑1 (MQ=255)

                                 gttggtggtgtGCACCGTGGTCGGTTTTGGTTTCCTTATTTACGGGGTGGTGCCGGGCATTCCGCTGATCCGTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGTCCGATGCTGTGGCGCTCGCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGa                                                                                                                                                                                                               <  1:258733/237‑1 (MQ=255)

                                 gtggggggtggtC‑CCGTGGTCGTGTTTGGCTTCCTTATTTACTGGGTGGTGCCGGGCACTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGGGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑t                                                                                                                                                                                                             <  1:1118989/233‑1 (MQ=255)

                                 ggttggtgttgtcctcGTGGTCGGGTTTGGGTTCTTTATTTGCTGGGTGGGCCCGGGGCTTCCGCTTTGCCCGCCCTTTGCGCTGGCGGCGGTGCGTGCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCGCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGa                                                                                                                                                                                                               <  1:425397/224‑1 (MQ=255)

                                  ctggTGGTGGTCCCCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGa                                                                                                                                                                                                               <  2:840758/238‑1 (MQ=255)

                                         tggtCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑GAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCCGGGGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGG‑TGGGGGCg                                                                                                                                                                                                      >  2:214191/1‑239 (MQ=255)

                                         tggtCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGGGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCCTTGAAGTTTGCCGTATC‑AGG‑GGCGA‑TGGGGACg                                                                                                                                                                                                      >  2:130920/1‑239 (MQ=255)

                                          ggtCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACg                                                                                                                                                                                                      <  2:980863/238‑1 (MQ=255)

                                             ccccGTGGCCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACgatg                                                                                                                                                                                                   <  2:1275595/236‑1 (MQ=255)

                                             aggcagggTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACgat                                                                                                                                                                                                    <  2:1119505/231‑1 (MQ=255)

                                                  tGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGGCCTCTCCGGGATTGTTGGCGGAGTGCGCATCCCG‑AAAAAAAACATGGGCCTTTTTGTGGGCGAAGCTGTGTGT‑GATTATGCCGCCGGTCTTGTGTCCTTGGAGTTTGCCGAAGT‑agt‑ggggagtgggggctgtatctt                                                                                                                                                                                               >  1:902295/1‑228 (MQ=255)

                                                              gCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGGTTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATACTGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGAAGCCTCCGGGTTGGGGTCGTTGAAGTTTCCCCGAGA‑CGT‑GGGGA‑GTGGGGCGATGGTTTTCCCCgcgggtcct                                                                                                                                                                                 >  1:1119505/1‑231 (MQ=255)

                                                                  ccTTATTTCCGTGGTGGTGCCGGGCATTCCCGTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGa                                                                                                                                                                            <  2:949008/239‑1 (MQ=255)

                                                                  ccTTATTGTCTGGGTGGGGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGa                                                                                                                                                                            <  2:945509/239‑1 (MQ=255)

                                                                       tttGCTGGGTGGTGTCGGGCATTCCGCTGATCCCTGCCTTTGCGCTTGCGGCGGTGCTTTCTCCTACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAg                                                                                                                                                                                                                                    <  2:311043/182‑1 (MQ=255)

                                                                         tACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGGCGATGATCTTTCCCGTCGGCGGT‑GCGACGGGCGa                                                                                                                                                                     >  2:354512/1‑239 (MQ=255)

                                                                             gggTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑TCGACGGGCGAAttt                                                                                                                                                                 >  2:421493/1‑239 (MQ=255)

                                                                             gggTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGGCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGCCGGCCGAAttt                                                                                                                                                                 >  2:632653/1‑239 (MQ=255)

                                                                              ggtggtGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGCCCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAATGGCGCATCCCG‑AAAAAAATAATGGGCCTTTTGCCGGGCGAAGCGTTGATG‑CGTGACGCCTCCGGCCTGGGGTCGTTGAAGATTGCCTTGGA‑AGT‑GGCGA‑TGGGGACGAGCGTCTTCACCGTcggcggggggcgcgtcgcaattc                                                                                                                                                                 >  1:874638/1‑222 (MQ=255)

                                                                                tggtgCGGTGCATTCCGCTGGTCCGTGCCTTTGCGCTGGGTGCGGGGCTTTGCCCGACCGAGGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCAGCCCG‑AAAAAAATCATGGGCAGTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGGCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTAt                                                                                                                                                               <  1:84788/238‑1 (MQ=255)

                                                                                tggtgCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGCAGTTTGCCGTAGC‑AGA‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGagtggcgactttttg                                                                                                                                                              >  2:837602/1‑225 (MQ=255)

                                                                                tggtgCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTAt                                                                                                                                                               <  2:717619/238‑1 (MQ=255)

                                                                                   tgCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCCTTTTGCAGGGCGCAGGGTTGCTG‑CATGACGCCTCCGGTCTGGTTTCGTTGAGGTTTGCCGGAAC‑GGG‑GGGGA‑GTGGGGCGATGGTCTTCCCCCTGGGCGGG‑GCGCCggggggattttgtaaa                                                                                                                                                           >  1:1239962/1‑225 (MQ=255)

                                                                                          cATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGtt                                                                                                                                                                                                                                  <  2:646233/166‑1 (MQ=255)

                                                                                           aTTCCGTTGATCCTTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGGGGGGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCa                                                                                                                                                   <  2:532189/238‑1 (MQ=255)

                                                                                             tCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGG‑TCGGCGGTGGGATTTTTGGAAGTA‑GCCATTg                                                                                                                                                >  2:202519/1‑239 (MQ=255)

                                                                                             tCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTACC‑AGT‑GGCGA‑TGGGGGCGATGATCTTCACCGTCGGCGGG‑GGGCCGGTCGAATTTATGAAAGTA‑GCCATTg                                                                                                                                                >  2:1149886/1‑239 (MQ=255)

                                                                                             tCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCCACCGATGCTTTGGCGCTCTCCGGGATTGTTGGCGAAGGGCCCATCCCG‑AAAAAAATCCTGGGCATTTTGCAGGGCGAAGCGTTGTAT‑AATGCACCCCCCGGTTGGGGTTCGTTCAAGGTTGCCGTAACAAGG‑GGCG‑‑TGGGGGCCGGGGTCTTCACCCTC‑GCGGTGGGGACAGGGGACTTTATGAAATAA‑GCCAtgg                                                                                                                                                >  1:1318048/1‑237 (MQ=255)

                                                                                                gCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGCAGGGCGCATCCCG‑AAAAAAATCATTGGCATTTTGCAGGGCGAATCGTTGATG‑AATGACGCCCCCGGGCTGGGGTGGTGGACGTTTTGCGCAGC‑AGG‑GGCGG‑TGGGGACGATGATCTTCACCGTGGGCGGG‑GGGACGGGCGTATTTATGAAAAGA‑GCCATTgggg                                                                                                                                             >  1:325340/1‑237 (MQ=255)

                                                                                                    aTCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGAA‑GCCAATGGCGGGAt                                                                                                                                         >  2:1215361/1‑239 (MQ=255)

                                                                                                         tGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGAAGGGCGGATTTTTTAAAGGA‑GCCCTTGGCGGGATttt‑c                                                                                                                                    >  2:909905/1‑238 (MQ=255)

                                                                                                         tGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGGCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGCAGATGATCTTCCCCGTCGGCGGG‑GCGACGGTCGAATTTATGAAAGTA‑GCAATTGGCgtgattttc                                                                                                                                     >  2:1272027/1‑230 (MQ=255)

                                                                                                          gCCTTTGCGCTGGCGGGGGTGCTTTCGCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGc                                                                                                                                  <  2:678239/239‑1 (MQ=255)

                                                                                                              ttGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGCTGGCGTAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTTCAGGGCGAAGCGTTGCTG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCTTAGC‑AGT‑GGCGC‑AGGGGGCGATGAACTTCACCGTCGGCGGT‑GCGAGGCGCGGATTTATGAAAGTA‑GCCATTGACGGGAtgttaccccgg                                                                                                                               >  1:253588/1‑229 (MQ=255)

                                                                                                               tGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCCCCGTCGGCGGT‑GGGACGGTCGAATTTTTTAAAGGA‑GCCCTTGGCGGGATTTT‑CGCCgggt                                                                                                                             >  2:579105/1‑237 (MQ=255)

                                                                                                                 gctcgggggggtggttttCTCCCACCGATTGGGGGGCGGCCTCCGGGATTTGTGGCGAAGGGCCCATCCCG‑AAAAAAATCATGGGCATTTTGCCGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGtttt                                                                                                                           <  1:1169817/224‑1 (MQ=255)

                                                                                                                   cTGGCGGCTGTGCTTTCCCCGACCGTTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCCTCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TTGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTtgt                                                                                                                         <  1:421493/239‑1 (MQ=255)

                                                                                                                     ggcggcGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGGTG‑AATGACGCCTCCGGCTCGGTGTCGTTGAAGTTTGCCGGGGC‑AGT‑GGGGG‑TGGGGAGGAGGATTTTCCCCGTCGGGGGT‑GGGGAGGGCGGAGTTTTGAAAGAACGCATTTGGGGGGATTCT‑CCCGGGgttttgg                                                                                                                        >  1:1004227/1‑236 (MQ=255)

                                                                                                                        gggggTGTTTCCCCCCACCGTTGCTGTGGCGCTCTCCGGGATTGTTGGGGAAGGGCGCATCCCG‑AAAAAAAGCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGa                                                                                                                    <  1:1215361/236‑1 (MQ=255)

                                                                                                                          cggTGCTTTTCCCGACCGATGCTGTGGCGCTCTCCGGGATTGGTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAg                                                                                                                   <  2:472144/238‑1 (MQ=255)

                                                                                                                                  tcgccgaccgaTGCTGTGGCGCTTTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTa                                                                                                          <  2:1131887/236‑1 (MQ=255)

                                                                                                                                       gaccgaTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGTTGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTTT‑TCCCGGGTTTGTGGGGGGACGGCTGTaacgt                                                                                                      >  2:276313/1‑234 (MQ=255)

                                                                                                                                       gaccgaTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGCATTTT‑TTCCGGTTTTgtggggggccgcacttatggt                                                                                                      >  2:895084/1‑221 (MQ=255)

                                                                                                                                       gaccgaTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGGCGAATTTATGAAAGTA‑GCCATTGGCGGTTATCT‑CGCCCGTTTTGTTGTGAgctgcgcgtacgtg                                                                                                      >  2:474921/1‑228 (MQ=255)

                                                                                                                                         ccgaTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGACTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGAA‑GCCATTGACGGTATTCT‑CGCCGGTTTTGTGGTGTgcgggcgtgccagtcg                                                                                                    >  2:387231/1‑228 (MQ=255)

                                                                                                                                         ccgaTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCCCCGGGCTGGTGTCGTTGACGTTTCCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGGGGA‑GCGAGGGTGGAATTTATGAAAGTA‑GCCATTCGGGGCATTTT‑TCTCGGGTTTgtggtggttggggggtcgcgcccg                                                                                                   >  1:665131/1‑221 (MQ=255)

                                                                                                                                          cggtGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGtcgct                                                                                                  <  2:1000694/236‑1 (MQ=255)

                                                                                                                                          cgaTGCTGTGGCGCTCTCCGGGATTGTGGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGtcgct                                                                                                  <  2:368196/239‑1 (MQ=255)

                                                                                                                                            aTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGTCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGAATTTTCCCCCGGTTTTG‑GGTTGGCTGGCTGTACgtggcccc                                                                                                 >  2:579641/1‑231 (MQ=255)

                                                                                                                                            aTGCTGTGGCGCTCTACGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGCCTGGTGTAGTTGAAGTTTGCCGTAGC‑AGG‑GGCGA‑TGGGGACGATGAACTTAACCGTCGGCGGT‑GGGAGGGTGGAATTTTTGTACGAA‑GCCATTGGAGGGATTAT‑TCCCGGTGTTTGGGGGAggtggacggtcggggcacg                                                                                                 >  1:235124/1‑224 (MQ=255)

                                                                                                                                                  tgGCGCTGTCCGGGTTTGTTGGGAGAGGGGCCACACCG‑AAAAAAATCATGGGCATTTTGCGGGGCGAAGCGTGGCGG‑AATGACGCCTCCGGTTTGGGGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGa                                                                                          <  1:609034/239‑1 (MQ=255)

                                                                                                                                                       ctctCGGGGGTGGTTGGCGAAGGGGGTATCCCG‑AAAAAAATCAGGGGCTTTTGGCGGGGCGAGGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGGGGT‑GGGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCt                                                                                     <  1:870201/239‑1 (MQ=255)

                                                                                                                                                         ctcCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGGATTTT‑CGCCGGGTTTTTGGTGGGCTGGCGGTACGGGCGCCCGCTGCGGTTtctc                                                                                    >  2:962010/1‑238 (MQ=255)

                                                                                                                                                          tcCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTTT‑TGCCGGGTTTTTGGGGGGCTGGCGTGCCGGTCGCTCCCTCCGGTTCCTCa                                                                                   >  2:98682/1‑238 (MQ=255)

                                                                                                                                                                 ttgttgGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTg                                                                           <  2:416951/238‑1 (MQ=255)

                                                                                                                                                                   tttgGCGGAGGGGGCTTTTCG‑AAAAAAAATAGGGGCGTTTTGAGGGGGGAAGGGGTGGTG‑GATGGGCGTTCCGGGATGGGTTCGGTGAAGGTTGCCGTAGA‑AGG‑TGCGA‑TGGGGACGATGGTCTTTACCGTTGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCAGTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTgggg                                                                        <  1:474921/238‑1 (MQ=255)

                                                                                                                                                                   tttgGCGACTGGCGCTGCCCG‑AAAAAATTCTGGGGCGTTTTGCGGGGGGTAGCGTTGGGG‑ATTGACCCGTCCGGGCTGGTGTCGTTGAAGTTTGCCGTAGC‑CGT‑GGCGA‑GGGGGGCGATGATCTTCACCGTCGGCGGT‑GCGAGGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTAGTCT‑CGCCGGTTTTGTGGGGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTgggg                                                                        <  1:1264024/238‑1 (MQ=255)

                                                                                                                                                                    ttgGGGAAGGTCGCTTCCCG‑AATAAAATCTGGGGATTTTTACGGGGGGATGCGTTGATG‑AATGACGCGGCTGGTTTGGTGGCGTTGAAGTTTGGCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGGCGGGGGT‑GCGGCGGTCGAATTTATGGAAGTA‑GCCATGGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGc                                                                       <  1:387231/239‑1 (MQ=255)

                                                                                                                                                                    ttgGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTTT‑CGCCGGGTTTGTGGTGAGTTGGCTGTACGGTGCCTCGCTCCGCTTCCTCAGCCC‑CTggggg                                                                       >  2:330398/1‑238 (MQ=255)

                                                                                                                                                                    ttgGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGGGATGGCGGGACGGTCGCTCGCTGCGCTTCCTCAGcccgtcgggc                                                                        >  2:800206/1‑231 (MQ=255)

                                                                                                                                                                    ttgGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCCGTTTTGTGGGGGGCTGGCTGTTCGGTTCCTCGCCGCGATTTCTCACCCC‑CCGGGGc                                                                       >  2:289687/1‑239 (MQ=255)

                                                                                                                                                                             ggCGCATCCCG‑AAAAAAATCAGGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGtgatga                                                               <  2:764789/238‑1 (MQ=255)

                                                                                                                                                                                  atgccG‑AAAAAAATTATGGGCATTTTGCAGGGCGAAGCGTGTAGG‑AATGACGCCTCCGGTCTGGTGTCGGTGAAGTTAGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGCTCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCcg                                                          <  2:86785/235‑1 (MQ=255)

                                                                                                                                                                                  aTCCCG‑AAAAAAATCGTGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCcg                                                          <  2:739532/238‑1 (MQ=255)

                                                                                                                                                                                  aTCCCG‑AAAAAAATCCTGGGCATTTTGCCGGGCGAAGCGTTGATG‑AATGGCGCCTCCGGTCTGGTGTCGGTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCcg                                                          <  2:665131/238‑1 (MQ=255)

                                                                                                                                                                                     ccG‑AAAAAAATCATGGGCATGTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGGCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGAc                                                      <  2:604163/239‑1 (MQ=255)

                                                                                                                                                                                      cG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TAGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGGCGAATTTATGAAAGTA‑GCCCTTGGCGGGGTTTT‑TGCCGGGTTTTCTGTTTGGTGGTGGTACGGGCGCCCGCTGCGATTCCTCTGCCG‑TTTGGGCGGGGCGGAAACcgcgcaa                                                     >  1:226234/1‑236 (MQ=255)

                                                                                                                                                                                       g‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGGTTTGTGGGGAGCTGGCTGTACGGTCGCTCGCTTCCGTTCCTCAGCCC‑CTGGGGCGGGGGTGAAccccccagcc                                                    >  2:256953/1‑232 (MQ=255)

                                                                                                                                                                                          aaaaaaGCATGGGCATTTTGCAGGGCGAAGGGTTGATG‑AATGACGCCTCCGGTCGGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCa                                                   <  2:902295/238‑1 (MQ=255)

                                                                                                                                                                                                 cATGGGCATTTGGCAGGGGGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTcc                                           <  2:1004227/239‑1 (MQ=255)

                                                                                                                                                                                                   gtggttttttGC‑GGGGGGAGCGTTGTAGAAATGGCGGCTCCGGTTGGGTGTGTTGGAAGTTTGCCGTAGC‑AGT‑GGGGA‑TGGGGACGATGATCTTCACCGGCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCctg                                         <  1:354512/233‑1 (MQ=255)

                                                                                                                                                                                                       cATTTTGCAGGGCGAAGCGTTGATG‑AATGGCGCCTCCGGTTTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCctgctg                                      <  2:468332/238‑1 (MQ=255)

                                                                                                                                                                                                            tGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGGCGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTCTTCT‑CGTCGGGTTTGTGGTGCGCTGGCTGTACGCGCGCTCGCGCCCGTTCCTCCGCCGACGGGGGGGGGGGGAAACCCGC‑ACGCAGaaccgcccgccgttc                                   >  1:468332/1‑222 (MQ=255)

                                                                                                                                                                                                               aGGGCGGAGGGTTGATG‑AATGGCGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCCCCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCctgct                              <  2:235124/238‑1 (MQ=255)

                                                                                                                                                                                                                    agaggcaggatg‑aATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCAtt                        <  2:909252/231‑1 (MQ=255)

                                                                                                                                                                                                                     gaaggtttggtgatgggcGCCCCCGGTTTGGTGTCGTGAAGGTTTGCCGTAGC‑AGT‑GGGGA‑TGGGGACGGTGATCTTCCGCGGCGGCGGG‑GCGGCGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGGGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCAtt                        <  1:276313/222‑1 (MQ=255)

                                                                                                                                                                                                                     aaGGGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCGCCGTCGGGGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCAtt                        <  2:1100613/238‑1 (MQ=255)

                                                                                                                                                                                                                     aaGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGGTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCCTCCCCGTCGGCGGT‑GCGACGGGCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCAtt                        <  2:1237810/238‑1 (MQ=255)

                                                                                                                                                                                                                          gtttgttaatggagCCTCCGGGTTGGTGTCGTTGAAGGTTGCCGTAGC‑AGG‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGct                    <  2:1296433/225‑1 (MQ=255)

                                                                                                                                                                                                                                  atgaCGCTTCGGGTTGGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCt            <  2:253588/238‑1 (MQ=255)

                                                                                                                                                                                                                                   tgaCGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAgctgg                                                                                                               <  1:675292/139‑1 (MQ=255)

                                                                                                                                                                                                                                   tgaCGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAgctgg                                                                                                               >  2:675292/1‑139 (MQ=255)

                                                                                                                                                                                                                                   tgaCGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGa          <  2:1305462/239‑1 (MQ=255)

                                                                                                                                                                                                                                         gctcGGTGTGGTGTCGTTGAAGTTTGCGGT‑GT‑GGTGGGCGT‑TGGGGACGGTTATCTTTCCCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGcc     <  1:962010/235‑1 (MQ=255)

                                                                                                                                                                                                                                         cTCCGGTTTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCg    <  2:1239962/239‑1 (MQ=255)

                                                                                                                                                                                                                                          tCCGGTTTGTTTTCGTTGAAGTTTGCCTTGGC‑GGT‑GGCGG‑TGGGGACGATGATCTTGCCCGTCGGGGGT‑GCGCGGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGGTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGa   <  1:765384/239‑1 (MQ=255)

                                                                                                                                                                                                                                          tCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCCCCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGa   <  2:744954/239‑1 (MQ=255)

                                                                                                                                                                                                                                          tCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCCCCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGa   <  2:325340/239‑1 (MQ=255)

                                                                                                                                                                                                                                           ccGGTTTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCCCCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGaa  <  2:1152686/239‑1 (MQ=255)

                                                                                                                                                                                                                                               tCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCcg                                                          <  2:467316/179‑1 (MQ=255)

ACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATG‑AATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGC‑AGT‑GGCGA‑TGGGGACGATGATCTTCACCGTCGGCGGT‑GCGACGGTCGAATTTATGAAAGTA‑GCCATTGGCGGTATTCT‑CGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCG‑CTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAA  >  NC_000913/4280218‑4280689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A