Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F18 I0 R1 274 115.5 2655328 86.7% 2302169 226.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,563,969 G→T 12.3% A241E (GCA→GAA)  yjiL ← putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,563,9690GT12.3% 267.5 / 3.3 111A241E (GCA→GAA) yjiLputative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehydratase
Reads supporting (aligned to +/- strand):  ref base G (35/58);  new base T (15/2);  total (51/60)
Fisher's exact test for biased strand distribution p-value = 1.21e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 112 total aligned reads displayed.

GACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAA  >  NC_000913/4563733‑4564203
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            
gccccacccctccccGGGACCCCCCTTTTTATTCCCCCGCCGGGGGAACAGGGCAGCCAAAGCCA‑AACCCCGCGGCCGCCGGTT‑AAATCAGGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGTTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTgcg                                                                                                                                                                                                                                            <  1:731451/228‑1 (MQ=255)
  cTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCCCT‑CGTTTGCCA‑AATAACCCCCCGCCCATAGGGcc                                                                                                                                                                                                                                          >  1:440972/1‑239 (MQ=255)
      atcagCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGAAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCa                                                                                                                                                                                                                                      <  2:1001997/238‑1 (MQ=255)
       tcagCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTATATGACGCCGACCGTGGAGTG‑AAATAAAACTAAAAACTCTTTCATCGGCGTGT‑CCCCACC‑CAGTTACCA‑ATTACCCCCGCGCCAAATGCGCCCGCaa                                                                                                                                                                                                                                     >  2:717612/1‑239 (MQ=255)
            ccAGGGATCCCCCATTGTATTCCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTg                                                                                                                                                                                                                                 <  2:1304149/238‑1 (MQ=255)
                 gaccccccatgtttgtcccccGCCGGGGGGACAGGTCCGCCAAAGGCA‑TAACCCGCGGCCGCCGGGT‑AAATCACGGGGAATAGCGCTGACGCGAAAAGTCAGGCCCGCTGCTTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCAt                                                                                                                                                                                                                            <  2:265442/218‑1 (MQ=255)
                     cccatttGTATTCCCCCGCGGGGGGGACAGGTCAGCAAAATCAA‑TACCCCGCAGCCGCCGCGT‑AAATCAGGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGTTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTCCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGa                                                                                                                                                                                                                       <  2:522654/234‑1 (MQ=255)
                     cccagtggTATTCCCCCGGCGGGGGGACGGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCCCGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGa                                                                                                                                                                                                                       <  2:661455/232‑1 (MQ=255)
                     aCCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCCTGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGTCGCCGACACT‑GCGTGAAAATAAAAATAAAAACTCTTTCATCGGCGCGT‑CCCCCCC‑CGTTACCCC‑ATTCCCCCCCCTCCAACTTCGCACCCAAATTgcgctccgcgg                                                                                                                                                                                                                       >  1:1209456/1‑232 (MQ=255)
                     aCCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGGGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGG‑CCTCCCT‑CCTTGCCCA‑ATTCCCCCCCCCCCAATTTGCCCACCAAATTGGGCaaccggg                                                                                                                                                                                                                       >  1:1172871/1‑233 (MQ=255)
                     aCCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTTCCCCACCCAATTTGGCATCAGGa                                                                                                                                                                                                                       >  2:1299658/1‑239 (MQ=255)
                     aCCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCCAATTGCGCATCAGGa                                                                                                                                                                                                                       >  2:322359/1‑239 (MQ=255)
                     aCCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCCCCAATTTCGCCAGCAAATTgcgatccagga                                                                                                                                                                                                                       >  2:844859/1‑231 (MQ=255)
                     aCCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTAACCCCGCGCCAATTTCGCCCGCaaaattgccatcatga                                                                                                                                                                                                                       >  2:1024113/1‑226 (MQ=255)
                     aCCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGGTTGTATGCCGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACAA‑CTTAACGCCCCCCCAAATGCAACACCAAAATGCGCGACAGGa                                                                                                                                                                                                                       >  1:649471/1‑239 (MQ=255)
                        cATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAAGGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATgg                                                                                                                                                                                                                    <  2:1085923/239‑1 (MQ=255)
                          ttGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATGAAAATAAAAACTCTTTCATCGGCGTGG‑CCCCACT‑CTTTGACCA‑CTTACCCCCCCCCCAAATATGCCCGCAAAtatgccacatggagggga                                                                                                                                                                                                                  >  1:1054291/1‑222 (MQ=255)
                          ttGTATTTCCTCCGCGGGGGGAAAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGCCCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGt                                                                                                                                                                                                                  <  2:1011364/239‑1 (MQ=255)
                           tGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AACTCACGGGGACTATCGCTGCCGCGAAAAGTCCTGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGGGTG‑AAATAACAATAAAAACTCTTTCATCGGCGTGT‑CCTCACC‑CGCTAGCCA‑ATTACATCCCCCCCAATTGCCCCACCAAATTtcgctcacggaggggca                                                                                                                                                                                                                 >  1:807832/1‑226 (MQ=255)
                              aTTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATACAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACC‑CGTTGTCCA‑AATAACCCCCCGCCAAATTCGCCAGCaaaatttcccacaagaaggggaatt                                                                                                                                                                                                              >  1:449055/1‑217 (MQ=255)
                                 tCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CCTTTGCCA‑ATTACCCCCGCGCCAATTTGCCCAGCAAATTTCGCATCCAGCGGGGGATTTAca                                                                                                                                                                                                           >  2:847180/1‑238 (MQ=255)
                                  ccTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGGGCATCAGGATGGGTATTTACCg                                                                                                                                                                                                          >  2:1249157/1‑239 (MQ=255)
                                  ccTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCCCGCCAATTGCGCCAGCAAATTGGGCATCAGGATGGGGATTTTCCg                                                                                                                                                                                                          >  2:1295012/1‑239 (MQ=255)
                                    cggcgggggggAAGTGTCAGCCAAATGAA‑TAACCCGCAGCCGCAGGGT‑AAATCACGGGGACTATCGCTGCCGCGAAAAGTCATGCCCGCTGCCGTCAACGCTTTGCGTGTTTGTATGCCGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑c                                                                                                                                                                                                      <  1:27451/234‑1 (MQ=255)
                                     cGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCCCT‑CGTTGACCA‑ATTACCGCCGCGCCCATTGCGCCCCCAAATTGGCCATCCGGATGGGGATTTACCG‑C‑Ca                                                                                                                                                                                                     >  2:544504/1‑239 (MQ=255)
                                               gcAGGTCA‑CCCAATGCACAAACCCGCACCCGCCGGGA‑AAACCACGGGGAATATGGCTGACGGTAAAAGCACGGCCCGCTGCTTAAAACGCTTTGCGGGTTTGTATGACGGCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CTTCACT‑CGTTGACAA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGt                                                                                                                                                                                            <  1:36573/237‑1 (MQ=255)
                                                    tCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGAt                                                                                                                                                                                       <  2:449055/238‑1 (MQ=255)
                                                    tCAGCCAAATGCA‑AACCCCGCAGCCGCCGCGT‑AAATCCCGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTTCAACGCTTTGCGGGTTTGTATGCCGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCCCT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTg                                                                                                                                                                                           <  2:310034/234‑1 (MQ=255)
                                                        ccAAATGCA‑TCACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCAGGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGCCGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGCAGCGC‑CCT‑ACTCCGATGACCA‑ATACACCCCCCGCCAATATCGCGAGGAAATCTCGCACAAGGATGGGTATTTACCG‑G‑CATTACCAgggggagtcgcc                                                                                                                                                                                   >  1:1305019/1‑228 (MQ=255)
                                                           aaTGCA‑TAACCCGCAGCCGCCGCGT‑AAATCCCGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTGCCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCAt                                                                                                                                                                               <  2:440972/239‑1 (MQ=255)
                                                             tGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGGTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCCCACT‑CCTTCACCA‑ATTACCGCCCGGCGCAATGCCCCAGCAAAAATCGACTACGAGTGGGTATTTACCC‑G‑CATTCCCGGGGGAGAGTCACGCATcc                                                                                                                                                                             >  1:964326/1‑239 (MQ=255)
                                                             atgactAACCCGCCGCCGCC‑CGTGAACTCCTGGGGAATGTGGTTGAAGAGAAATTCCAGGCCGGCGTCCTGCAACTATTGTCGGGTTTGGATGATCCCGACCTTGGAGTA‑AAATAAAAATACAAACCTTTTCATCGGCTGGT‑CTTCACT‑CGTTGCCAA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATc                                                                                                                                                                              <  1:199492/234‑1 (MQ=255)
                                                                   cccccGCCCCCCCCGAGT‑AAATCCAGGGGAATTTGGCGTGGGCGAAAAGTAAGCCCAGTTGCCTGCAACTCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAAAAAAAATAAAACCTCTTTCATCGGCGGGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCa                                                                                                                                                                        <  1:1299658/237‑1 (MQ=255)
                                                                   aaCCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATCAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑AATACCCCCGCGCCAACTTCCCCCGCAAACTCCGCATCAGGGAGGGGATTTACCG‑G‑CATTCGCCGGGGGGCTTCCAGCAACCggggc                                                                                                                                                                        >  1:177174/1‑237 (MQ=255)
                                                                        gacgccgccGGTT‑AAACCACGGGGAATATCGCTGACGGGAAAAGTCAGCCCCGCTGCCTGAAACGCTTGGCGGGTTTGTATGCCGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACtt                                                                                                                                                                   <  2:1291147/236‑1 (MQ=255)
                                                                        gCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGAACGTGGAGTG‑AAATACAAATAACAACTCTTTCATCGGCGTGT‑CCTCACT‑TGTTGTCCA‑ATTACCGCCGCGCCAATTGCGCCCGCAAATTGCGCATCAGGGTAGGTATTTTCCG‑G‑CATTCACCGGGGAGGTACCCGCACCCGGGCaaaat                                                                                                                                                                    >  1:113596/1‑236 (MQ=255)
                                                                         cAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGCCGTGT‑CCTCACT‑CGTTTACCA‑ATTACCGCCGCGCCCATTTCGCCAGCCAATAGCCCACCACAGTGGGTATTTAACG‑G‑CCGTCTCCGGAGAGATTCCAGCATCCGGGCAAACttt                                                                                                                                                                  >  1:799806/1‑238 (MQ=255)
                                                                         cAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGCTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGCCGTGT‑CCTCACC‑CGTTGACCACATCACC‑CACCGCCAATTGCTCACGCCACATGTCGAGTAGGAGGGGTATTTACCGCG‑CTCTC‑CCGGTGAGATTCCACCACCCGGGaaaaaaga                                                                                                                                                                  >  1:1318535/1‑235 (MQ=255)
                                                                               ccgcGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGGGT‑CCTCACT‑CGTTTACCA‑ATTACCGCCCCGCCCATTTCGCCAGCAAATTGCGCCTCCGAGAGGGGATTTACCG‑G‑CATTCCCGGGGGGGATTCCAAGACCCGGGCaaaactttgggaa                                                                                                                                                            >  1:1299559/1‑229 (MQ=255)
                                                                                          ccgggggAATTGCGCTGACGCGAAAAGTCATGCCCGTTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGc                                                                                                                                                  <  2:177174/236‑1 (MQ=255)
                                                                                          ccggggaAATATCGCGAGCGCGAAAAGTCATGCCCGCTCCCTGAAAGGCTTTGCGGGTTGGTATGCCGCCGACCGTGGAGTG‑AAAAAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTGCCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGc                                                                                                                                                  <  2:172231/236‑1 (MQ=255)
                                                                                                           agtggataaaaagacacggCTGCCGGCAACGCTTTGCGGGTTTGTATGACGCCGACCTTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAAt                                                                                                                                  <  2:953071/220‑1 (MQ=255)
                                                                                                           aCGCGCAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACAGCCGCGCCAATTGAGCCAGCAAATTCGGCATCAGGATGGGGCATTACCG‑G‑CATTCCCAGGGAGGATTACTGCAGCCGGGCAAACTTCTGGCCATGACAAACGCAACAAgccaaaaaaaga                                                                                                                                 >  1:212384/1‑228 (MQ=255)
                                                                                                           aCGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCCCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGAGTTTACCG‑G‑CATTCCCAGGGGGAATTCCACACTCCCGGGACACTTTTGGCAACCCCTAACGccccaccaaaacaaaac                                                                                                                                  >  1:727974/1‑223 (MQ=255)
                                                                                                             gcgAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGTTTTGTATGACGCCGACCGTGGAGTA‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCgg                                                                                                                               <  2:1172871/239‑1 (MQ=255)
                                                                                                             cagAAAAGTCCTCCCCTGTGCCTGCAACGTTTGGCGGTTTTTTATGGCGCCAGCCGTGGAGGG‑AAAAAAAAATAAAAACCCTTTCATGGGGGGGC‑CCTCACC‑GGTTGACCA‑ATTGCCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCAGCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCgg                                                                                                                               <  1:393184/237‑1 (MQ=255)
                                                                                                              cgAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAACCGCTTGCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACtt                                                                                                                                                                   <  2:299955/202‑1 (MQ=255)
                                                                                                              ataaaaGTCATGCCCGCTGCCGACAACGCTTTGGGGTTTTGTATGCCGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCAGCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCgg                                                                                                                               <  2:799806/236‑1 (MQ=255)
                                                                                                               gAAAAGTCAGTCCCGCTGCCTGGCACGCTTTGCGGGTTTGTATGCCGCCGACCGTGGATGG‑AAATAAAAATAAAAACTCTTCCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGgcg                                                                                                                             <  2:702176/239‑1 (MQ=255)
                                                                                                               gAAAAGCCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGCCGCCGGCCGTGGAGTG‑AAAAAAAAATAAAAACTCTTTCATCGGCGTGG‑CCTCCCT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGgcg                                                                                                                             <  2:1209456/239‑1 (MQ=255)
                                                                                                               caaaaGTCATTCCCGCTGCCTGCAACGTTTTGGGGGTTTGTATGACCCCAACCGGGGATGA‑AAATAAAAATAAAAACCCTTGCATCGGCGTGT‑CCTCACG‑CTTTGACCA‑ATTCCCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGgcg                                                                                                                             <  1:1295012/238‑1 (MQ=255)
                                                                                                                aaaaGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAACCTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGgcg                                                                                                                             <  2:1157464/238‑1 (MQ=255)
                                                                                                                        tGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGG‑CCTCACT‑CGTTGACCA‑ATTAACGCCCCGCCAATTTCGCCAGCCAATTTGGCATCAGGCAGGGTATTTACCG‑G‑CCTTCGCAGGTGGGATTCCAGCATCCGGGCAAAATTCTGGCAAAGa                                                                                                                                                         >  1:299955/1‑202 (MQ=255)
                                                                                                                              ttgcctgcAACGTTTGGCGGTTTTGTATGCCGCCGACCGTGGAGTG‑AAATAAAAATAAAACCTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGAc                                                                                                              <  2:1054291/238‑1 (MQ=255)
                                                                                                                               tgcctgcAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGG‑CCTCCCT‑CGCTGACCA‑ATTACCCCCCCGCCAATTTCCCCCGCAAATTGCGCCCCAGGGCGGGATTTTACTG‑G‑ATTTTGCAGCGGCGCTTTCGCCCTCCTGGCCACCTTTCGGGAACGTCTAACGCCCCCAGAAACCAGAACACGCGCTTCCCCGgaagacc                                                                                                              >  1:946008/1‑233 (MQ=255)
                                                                                                                                          cTTTGCGGTTTGTTATGACCGCAACCGTGGAGTT‑AAATAAAAAAAAAAACTCGTTAATCGGCGTTT‑CCTCCCC‑CATTGCCCA‑ATCACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAAGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAAt                                                                                                  <  2:232021/239‑1 (MQ=255)
                                                                                                                                               gggttttttatatgcccGCCCTTGGGGTA‑AAAAAAAAATAAACACCCTTTAGACGGCTGAC‑CCGCGCT‑CGTTGCCCA‑AGTTCCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCa                                                                                             <  1:1146377/224‑1 (MQ=255)
                                                                                                                                                     tGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCCCGCCCATTGCGCCAGCAAATTGCGCATCCGGGCGGGTATTTACCC‑G‑CATTCGCCGGGGGGATTCCCGCATCCGGGCAAACATCTGGCAATACCTAACGCCACCAGTAAACAGAATACGCGCTTCACAGGAGAAACGAGACAAGAAAATGGCAcaccc                                                                                        >  1:908257/1‑237 (MQ=255)
                                                                                                                                                       tATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAACCTCTTTCACCGGCGTGT‑CCTCACC‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGgcg                                                                                                                             <  2:659867/199‑1 (MQ=255)
                                                                                                                                                       tATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCCCCCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTTCCA‑G‑CATTCGCAGGTGAGATTCCACCATCCGGGCAAACTTTTTGCAATTCCTAAACCCACCAAGAAAAAGAAACGGCGCTTAACAAGAAAAACGAACAAAGaaaaagaaaaccccccc                                                                                     >  1:643057/1‑225 (MQ=255)
                                                                                                                                                       tATGACCCCCCCCGTGGAGGA‑AAATAAAAATAAAAACTCTTTCATCGGCGTTG‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCcg                                                                                     <  2:56409/239‑1 (MQ=255)
                                                                                                                                                            cgccgcCCGTGGAGTG‑AAATAAAAATAAAACCTCGTACATCGGCGGGT‑CCCAACT‑TGTTGACCA‑ATGACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCa                                                                                <  2:1054693/239‑1 (MQ=255)
                                                                                                                                                             gccgccCGTGGATTG‑AAATAAAAATAAAAACTCTTTCATGGGCGTGT‑CCTGACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCa                                                                                <  2:521677/238‑1 (MQ=255)
                                                                                                                                                                 aCCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTTCGCCAGCAAATTGCGCATCCGGATGGGTATTTACCG‑G‑CCTTCCCCGGTGAGATTCCAGCATCCGGGCAAAATTTTGGCAATGACCAACTCCACCAGTAAAAAGAAACGGCGCCTCAACGGAGAAAAGGGCAATAAAAATGGGACACCCCCCGGCCaccccg                                                                           >  1:1270096/1‑234 (MQ=255)
                                                                                                                                                                       ggggtaaaaaaaaaataaAACCTCTTCCATGGGC‑TGTCCCTCACT‑CTTTGACAA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAAt                                                                      <  2:1304053/233‑1 (MQ=255)
                                                                                                                                                                       ggggtaaaaaaaaaaaaaaaCCCCTCCCAGCGGGGTGC‑CCTCACC‑CTGTACCAA‑ATGCCCGCCGCGCCAATTGCGCCATCAAATTGCCGGTCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAAt                                                                      <  1:395394/233‑1 (MQ=255)
                                                                                                                                                                             aaataaaaataaAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTTCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCCGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCCCCATCGCGTTAATCACt                                                                  >  2:381822/1‑237 (MQ=255)
                                                                                                                                                                             aaataaaaataaAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATTACTAACGCCCCCAGTAAAAAGAATCGGGCCTTCACAGGGGAGACGAGCCATTAAATTGGCACCCCTCCCGGCCCTCCCGGTAATCaacc                                                                 >  2:395394/1‑235 (MQ=255)
                                                                                                                                                                                               tttCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGGGAGATTCCAGCCTCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAAAAACGGAGCTTCCCCGGCGGAACGAGCAGTGAAATTGGCACCCCTCCCCGCCACCCCGCTAAACACCCCCCCGAGAAAAGCTTCCgg                                              >  1:218609/1‑239 (MQ=255)
                                                                                                                                                                                               tttCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGAAGGGTATTTACCG‑GCCATTC‑CAGGGGAGATTCCCGCATCCGGGCAAACTTCTGGCAATGCCCAAGCCCCCCCGTAAACAGCAACGGCGCTTACCAGAGGAGACAAGCAATTAAATTGGGACTCCCCCCCGCCAACCCGTTAAACCCCCCCGCGAGaaaccctcttgc                                              >  1:1041965/1‑229 (MQ=255)
                                                                                                                                                                                                    cgggCGGGT‑CCTTACT‑CTTTAACCA‑ATTCCCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGGGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGa                                         <  2:946008/237‑1 (MQ=255)
                                                                                                                                                                                                         gggtggcctccccggTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGAc                                        <  2:1270096/223‑1 (MQ=255)
                                                                                                                                                                                                           gt‑CCTCACT‑CGTTGACCA‑ATTAACGCCGCGCCAATTGCGCCAGCAAATTTCGCATCAGGGTGGGTATTTACCG‑G‑CATTCGCAGGGGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGGAAACAGAAGCGGGGCTTCCCCGGAGAGACGAGCAATGACATTTGCACTCCTCCCCGCCACCCCGTTAAACACCCCCGCGAGAATCCCTTCGGGCGCGACcccccc                                  >  1:291411/1‑237 (MQ=255)
                                                                                                                                                                                                            gccctccct‑cTTTGACCA‑ATTCCCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGc                                  <  2:873950/237‑1 (MQ=255)
                                                                                                                                                                                                                   ctcctttGCCCA‑TTTACCGCCGCGCCAATTGCCCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAgcg                            <  2:472305/234‑1 (MQ=255)
                                                                                                                                                                                                                        ttGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGCGATTCCAGCATCCGGGCAAACTTCTGGCAATGCCTAACGCCACCAGTAAACAGACTCGGCGCTTACCAGGAGAGCCGAGCAATGAATTTGGCCATCCTCCGCCCCATCGGCTGAATCACCCCTGCGCGAATAGCATCTGGCGCCACGCCCCCCGGGCGCACGc                       >  1:527463/1‑239 (MQ=255)
                                                                                                                                                                                                                        ttGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCgg                                                                                                                               <  2:839095/135‑1 (MQ=255)
                                                                                                                                                                                                                        ttGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCgg                                                                                                                               >  1:839095/1‑135 (MQ=255)
                                                                                                                                                                                                                        ttGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGAACTCCTCCGCGCCATCGCGTTAATCACCCCTGCGAGAATCGCTTTTGGCGCGACCCCCCCTGAGCGCAGGc                       >  1:1125069/1‑239 (MQ=255)
                                                                                                                                                                                                                            ccA‑TTTCCCTCCGCGCCAATTGCGCCAGAAAATTGCGGATCAGGATGGGTTTTTACCG‑G‑CATTAGGAGGTGAGATTCCAGCATCCGGGCAAACTTCGGGCAATGACTAACGCCAACAGTAAACCGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGac                   <  1:1056463/239‑2 (MQ=255)
                                                                                                                                                                                                                            ccA‑ATTCCCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGAt                   <  2:1299559/239‑1 (MQ=255)
                                                                                                                                                                                                                            ccA‑ATGCCCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCAGCCGGGCAAACTGCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGAt                   <  2:113596/239‑1 (MQ=255)
                                                                                                                                                                                                                             cattgccccttcggcccAATTGCGGCCGCAAATTCTGCATCAGGATGGGTATTGACCG‑G‑CATTCGTAGGTGAGCTTCTAGCATCCGGGCAAACGTCCGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGGAAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCATCCTGAt                   <  1:198743/224‑1 (MQ=255)
                                                                                                                                                                                                                             cA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CCTTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGAt                   <  2:1318535/238‑1 (MQ=255)
                                                                                                                                                                                                                              cattgtcccccgccgcatttGCCCCGGCAAATGGCGCATCGGGAGGGGTATTTCCCG‑G‑CAGTCGCAGGTGAAATTCCAGAATCCGGGCAAAATTCGGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGGTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCGCCGCGCGAGCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATc                  <  1:1039077/221‑1 (MQ=255)
                                                                                                                                                                                                                                aTTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCACTCAAATTGGACCTCCTCCGCGCCATCGCGTTAATCACTACTGCGGAAATCCCTTCTGGCCCCGCGCCCCCTGGaggcaggcggtcc                  >  1:1280503/1‑232 (MQ=255)
                                                                                                                                                                                                                                aTTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGCATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATc                  >  2:1179087/1‑237 (MQ=255)
                                                                                                                                                                                                                                aTTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGGTc                  >  2:665769/1‑237 (MQ=255)
                                                                                                                                                                                                                                aTTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACCCCTCCGAGAATCGCTTCTGGCGCGCCCCCCCCGGCGCGCCGGCTGATCgg                >  1:1313172/1‑238 (MQ=255)
                                                                                                                                                                                                                                aTTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATAACTCCTGCGAGAATCGCTTCCGGCCCGACCCCCCCGGACAGCAGGATGATc                  >  1:651963/1‑237 (MQ=255)
                                                                                                                                                                                                                                       ccgcTCCAATTTGGCCAGAAAATTGCCCATCAGGGGGGGTATTTACCG‑G‑CATTCGGAGGTGAGATTCCAGCAACCGGGCAAACTTCCGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGAt         <  1:381822/239‑1 (MQ=255)
                                                                                                                                                                                                                                       ccgcGCCAATTTCCCACGCAATTGGCGCATCAGGATGGGTATTCTCCG‑G‑AATGCCCAGGTGAGAGTCTAGCCGCCGGGCAAACTCCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAAGTGGCACTCCCCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTTAGCGCAGGCTGATCGCTTCTGAt         <  1:396574/239‑1 (MQ=255)
                                                                                                                                                                                                                                       ccgcGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGAt         <  2:643057/239‑1 (MQ=255)
                                                                                                                                                                                                                                       cccccccAATTGCCCCAACAATTTGCGCATTAGGATGGGTATTTACCC‑G‑GATTCGCAGGGGAGATTCCAGCAGCCGGGCAAACGTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGAt         <  1:32777/234‑1 (MQ=255)
                                                                                                                                                                                                                                        cgcgCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑AATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGGCTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGGAATTGGCACTCCTCCGCGCCCTCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGAt         <  2:949279/238‑1 (MQ=255)
                                                                                                                                                                                                                                          cgcCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGCCGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTCCTGGCGCGACGCCCGCTGAGCGCAGGCTGGTCGCTTCTGATTc       >  2:617556/1‑238 (MQ=255)
                                                                                                                                                                                                                                           gcCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGGGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGAttat      >  2:1137747/1‑236 (MQ=255)
                                                                                                                                                                                                                                           gcCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAgcg                            <  2:221659/216‑1 (MQ=255)
                                                                                                                                                                                                                                             cAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCa   <  2:789875/239‑1 (MQ=255)
                                                                                                                                                                                                                                              aaTTGCGCCAGCAAATTGCGAATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCAGCCGGGCAAACGTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCaa  <  2:1322606/239‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            
GACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCA‑TAACCCGCAGCCGCCGCGT‑AAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTG‑AAATAAAAATAAAAACTCTTTCATCGGCGTGT‑CCTCACT‑CGTTGACCA‑ATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCG‑G‑CATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAA  >  NC_000913/4563733‑4564203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A