Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F18 I0 R1 274 115.5 2655328 86.7% 2302169 226.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,601,682 T→A 11.1% L139F (TTA→TTT)  yjjB ← DUF3815 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,601,6820TA11.1% 414.2 / 4.8 155L139F (TTA→TTT) yjjBDUF3815 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (96/37);  new base A (4/16);  total (102/53)
Fisher's exact test for biased strand distribution p-value = 1.15e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

TGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGAAGCCATAAAGGTTGACCACT  >  NC_000913/4601444‑4601920
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                
      aaCGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGCAGGGAGATAACTTTTATAGTAACACAC‑GTAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGTAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCC‑GGAATGGAAAGCCCGATGCT‑TAACGc                                                                                                                                                                                                                                             >  2:184756/1‑238 (MQ=255)
            ttGGTGATCGTTTCCCAGGGGGTCAATCCCAACGATGGCCGGGGTCAAAACGTGGGAAAGAATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATCTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑Ccaaca                                                                                                                                                                                                                                     <  1:505348/239‑1 (MQ=255)
             tGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTTTAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATAAGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAACCCGATGGA‑AAACGccgggccct                                                                                                                                                                                                                                     >  1:1106729/1‑231 (MQ=255)
                   tCGTGTAACAGGGCGTCAATACCAACGGCGTTCGGGGTCAAAACGCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑Taa                                                                                                                                                                                                                                                <  2:775657/222‑1 (MQ=255)
                    cGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGCGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCAAGGGCGCTTGCGGGACAACCATAATCAA‑GGAATGGAAAAACCGATGGG‑AAACGCC‑Ccaaaaacagaaaa                                                                                                                                                                                                                             >  1:928951/1‑232 (MQ=255)
                    cGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGCATGGAAAGACCGATGGA‑TAACGAA‑CCACCAATCGCTGa                                                                                                                                                                                                                             >  2:436849/1‑239 (MQ=255)
                        tACCAGGGCGTCAATCCCAACGGCGTGCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑t                                                                                                                                                                                                                                                  <  2:947096/215‑1 (MQ=255)
                        gaCCAGGGCGTCAATTCCAACGACGGCCGGGGTCAAAACTCTTGAAGACATATTGGTTGTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGGCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑t                                                                                                                                                                                                                                                  <  1:845803/214‑1 (MQ=255)
                             cggCGTCATTACCAACGACGTCCGCAGTCAAAACTCTGGAAGACATACTGTCTCTCAACTATGGAACGATGAACGAGAAGGGAGATAAATTTTATAGTAACACCC‑GGCGAAGAGGAGGCTGTCTCCCATCCGTGTGCTTAAGCGAGGCAACTTTATACGCGAGGGCGCTTACGGTACAACCATATTTCA‑GGAATGGAGAGACCGATGGA‑TAACGAC‑Acaacaactttctcaaataca                                                                                                                                                                                                                      >  1:775657/2‑223 (MQ=255)
                                cGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAACAACCGATGGT‑TAACGCA‑CCAACAATCGATGAATCTgc‑aagcaa                                                                                                                                                                                                                >  2:1292190/1‑239 (MQ=255)
                                  tCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTCTAAGCGAGGGGGCCTGCGGTACAACAATACTACG‑GGAAAGGAAAGACCGATGGA‑GAACGCA‑Ccaacctgtctcttaaactatccaaac                                                                                                                                                                                                                >  1:947096/1‑216 (MQ=255)
                                  tCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAACGCCCGATGGA‑AAAACCC‑CCCACAATCAATAAAGCTGT‑AAGAAAGt                                                                                                                                                                                                              >  1:859803/1‑239 (MQ=255)
                                  tCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCACCAACCGATGAAGCTGT‑AAGAAAGt                                                                                                                                                                                                              >  2:200237/1‑239 (MQ=255)
                                  tCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGa                                                                                                                                                                                                                             <  1:876616/225‑1 (MQ=255)
                                  tCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGa                                                                                                                                                                                                                             >  2:876616/1‑225 (MQ=255)
                                  tCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGAA‑CCAACAATCGATGAAGCTGT‑AAGaaaat                                                                                                                                                                                                              >  2:1266455/1‑237 (MQ=255)
                                  tCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGACGGA‑TAAGGCA‑CCAAAAAACGATGAACCTGT‑AAGaaaat                                                                                                                                                                                                              >  1:820958/1‑237 (MQ=255)
                                          gtcagatgtgtaaaagagacggctGGAAGCCATAGTGTGATTAAAATATGGGACGATGAACGGGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAAGAAGCTGGCTCTCATCCGTGGGCTTAAGCGAGGCAATTTTAGACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATTAAGCTGT‑AAGAAAGTTGGTTaa                                                                                                                                                                                                       <  1:333791/216‑1 (MQ=255)
                                          aaCGACGTCCGGGGTCAAAACTCTGGAAGACATACTGGTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAAc                                                                                                                                                                                                      <  2:677002/239‑1 (MQ=255)
                                          aaCGACGTCCGGGGTCAAAACCCTGGAAGACATACTGTTACCAAAATATGGAACGATGAACGAGAAGGGAGTAAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAAGCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAAc                                                                                                                                                                                                      <  2:524309/239‑1 (MQ=255)
                                                gTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTa                                                                                                                                                                                                >  2:64215/1‑239 (MQ=255)
                                                 tCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAAAAGGGCaa                                                                                                                                                                                               >  2:1307655/1‑239 (MQ=255)
                                                             aCTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGtt                                                                                                                                                                                   <  2:820958/239‑1 (MQ=255)
                                                                 tGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGGGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAAAAATCGATAAAGCGTT‑AAGAAAGTTGGTTAAACGGGAAAACACCAAAGGTTCGCt                                                                                                                                                                               >  1:198734/1‑239 (MQ=255)
                                                                 tGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGCTGGA‑TAACGCA‑CCAAAAAACGATGAAGCTGA‑AAGAAAGTTGGTTAACAGGGAAAACACAAAAGGTTccgt                                                                                                                                                                               >  1:162932/1‑236 (MQ=255)
                                                                 tGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCACCAATCGATGAAGCTGT‑AAGAAAGTTGGGTAACAGGGTAATCACCAACGGTTCCCt                                                                                                                                                                               >  2:458967/1‑239 (MQ=255)
                                                                 tGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCACCAATCGATGAACCTGT‑AAGAAAGTTGGTTAAAAGGGGAATCATCAACGGttacct                                                                                                                                                                               >  1:904545/1‑235 (MQ=255)
                                                                           aCTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAAc                                                                                                                                                                     >  2:374275/1‑239 (MQ=255)
                                                                                 tctcAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTg                                                                                                                                                                   >  2:178526/1‑235 (MQ=255)
                                                                                           ggAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGTTAATCACCAACGGTTCCCTGTAACCAAAATGGCTGATTTTTAcc                                                                                                                                                      >  1:579534/1‑238 (MQ=255)
                                                                                             aaCGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTAccgcc                                                                                                                                                   >  2:583421/1‑239 (MQ=255)
                                                                                                 aTGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTa                                                                                                                                                                                                >  1:130317/1‑190 (MQ=255)
                                                                                                  tGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGaaac                                                                                                                                              >  2:435618/1‑238 (MQ=255)
                                                                                                  tGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAAt                                                                                                                                              >  2:139232/1‑239 (MQ=255)
                                                                                                  tGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAAAAGGGCAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTAACCGCCGaaaa                                                                                                                                              >  1:449859/1‑238 (MQ=255)
                                                                                                       gagaTGGGTGATGACTTTTTTAGTAACCCAC‑GGAGAGGTGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTAGCCGGGAGGGGGCTGGGGATACACCCATAATCCA‑GGAATGGAAAGCCGGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTTT‑AAGAACGTTGGTTAACAGGGTAATCATCAACGGTACGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTg                                                                                                                                         <  1:375014/239‑1 (MQ=255)
                                                                                                       gaaaaGGGGGTTTACTTTTATAGAAACACCA‑GGGGGAGGGGGGGCTGTCTCTCATGCGTGTGCTTAAGCGAGGCAATTTTATCGGCGAGGGCGCTTGCGTTAAAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TACCGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACGGGCTGATTTTTACCGCCGAAATCATTg                                                                                                                                         <  1:862184/236‑1 (MQ=255)
                                                                                                         gaAGGGATATAACTTTTATAGTAACGCAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTg                                                                                                                                         <  2:198734/237‑1 (MQ=255)
                                                                                                                 aTAATTTTTATAGTAAC‑CACGGGAGGAGGGGAGGCTGTCTCTCATCCGTGGGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAAGCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAg                                                                                                                                <  2:837057/238‑1 (MQ=255)
                                                                                                                 aTAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATAATTGCGGTATAAGc                                                                                                                               >  2:259805/1‑239 (MQ=255)
                                                                                                                       tttATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCACCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGatat                                                                                                                         >  2:880774/1‑239 (MQ=255)
                                                                                                                        ttATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGCATAAGCCgtaat                                                                                                                         >  2:448728/1‑234 (MQ=255)
                                                                                                                           taGTAACACAG‑GGGGGAGGGGAGTCTTTCCCTCATCGGTGTGGTAAAGCGGGGAAATTTAATAGGGGGGGGCGCTTGGGGTAAAACCAAATTCCG‑GGAAGGGAAAGCCCGAGGGA‑AAACGCA‑CCAACAATCAATGAAGCTGA‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGTTTCGCTGTAACCTAACTGGCTGATTTTAACCGCCGAAATCATTGCGGTATAAGCCGATATGccc                                                                                                                     <  1:869962/239‑1 (MQ=255)
                                                                                                                               accacac‑GGTGGGGGGGAGGCTGTCTCTCTTCCGTGGTCTTAAGCGAGGAAATTTGATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAAGGGAAAGACCGATGGA‑TAACGCA‑CCAACAAGCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCggg                                                                                                                  <  1:739047/236‑1 (MQ=255)
                                                                                                                                acacac‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGaa                                                                                                                                                                                                                 >  1:212963/1‑142 (MQ=255)
                                                                                                                                acacac‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCCTTGCGGTAAAACCCGCAAAGCCCGGGaa                                                                                                                >  1:906014/1‑239 (MQ=255)
                                                                                                                                acacac‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAAAAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATAGGCCCGGGaa                                                                                                                >  2:301350/1‑239 (MQ=255)
                                                                                                                                    ac‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGCTGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGCAATCATTGCGGTATAAGCCGATATCCCCGGGAACATa                                                                                                            >  2:472257/1‑239 (MQ=255)
                                                                                                                                       ggaggaGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAgg                                                                                                          >  2:386436/1‑239 (MQ=255)
                                                                                                                                         aggagaggagGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGcc                                                                                                                              >  2:21982/1‑217 (MQ=255)
                                                                                                                                           gagaggagGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAATGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATa                                                                                                      >  2:714738/1‑239 (MQ=255)
                                                                                                                                              aggaggCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGCATAAGCCGATATGCCCGGGAACATAGGGATAACg                                                                                                   >  2:131209/1‑239 (MQ=255)
                                                                                                                                                gaggCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGCTTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACgg                                                                                                  >  2:535775/1‑238 (MQ=255)
                                                                                                                                                 aggCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGAAATCATCAACGGTTCGCTGGAACCTAACTGGCTGATTTTAACCTCCTAAACCATTCGCGTATAAACCCATATACCCCGGAACACAGGGaaaaacgc                                                                                                 >  1:127563/1‑234 (MQ=255)
                                                                                                                                                   gCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTGGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCACGGTAACATAGGGATAAAGgccg                                                                                               >  2:881975/1‑238 (MQ=255)
                                                                                                                                                   gCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGAAATGCCCGGGAACAAAGGGAAAACGgccgc                                                                                              >  1:447892/1‑239 (MQ=255)
                                                                                                                                                   gCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAAACTAACTGGCTGCTTTTTACAGCCGAAATCATTGCGGGATAAACCGATATGCCCGTGAACAAAGGGAAAACGgccgc                                                                                              >  2:654118/1‑239 (MQ=255)
                                                                                                                                                     tGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGCTAACGGCCGCCa                                                                                            >  2:376634/1‑239 (MQ=255)
                                                                                                                                                        ctctcATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGGTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGAAATGCCCGGGAAAATAGGGGTAACGCCCCCAACgg                                                                                         >  1:867160/1‑239 (MQ=255)
                                                                                                                                                           agaatcgggtctttAAGCGAGGCATTTTTATAGGGGAGGGCGCTGTGGGTAAACCAATAACCCA‑GGAATGGAAATACAG‑TGGATTAAACCA‑CAATAAATCTGTAAGGTTGA‑AAAAAAGTGGGTTAACTGGTAAATCATCACCGTTTCGTGTTATCCTACCTGGCTATTTTTTCCCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaa                                                                                      <  1:692080/227‑1 (MQ=255)
                                                                                                                                                             gggagattggtataagagacagaTTTTATACGCGGGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGAAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTaaa                                                                                     <  2:1307370/216‑1 (MQ=255)
                                                                                                                                                             aTCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGGATAAGCCGATATGCCCGGGAAAAAAGGGATAACGGCCGCCACGGTAAac                                                                                    >  1:1126122/1‑239 (MQ=255)
                                                                                                                                                                 gtgtGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCT‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGGAATCATCAACGGTTCCCTGTAACCTAACTGGCGGATTTTTACCGCCGAAATCATTGCGGGATAAGCCGAAATACCCGGGAACATAGGGATAAAGgccgcccaagaaaaaaaat                                                                                >  1:649078/1‑226 (MQ=255)
                                                                                                                                                                 gtgtGCTTAAGCGAGGCAATTTTATACGCGAGGACGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGCAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGAAAACACtt                                                                                >  2:1010303/1‑239 (MQ=255)
                                                                                                                                                                        tAAGCGGAGAAATTTTAACCGGGAGGGCGTTTGCGGTACCACCATAATCCG‑GGAATGAAAAGGTCGTGGGA‑AAACCCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGTTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATg                                                                         <  1:259805/239‑1 (MQ=255)
                                                                                                                                                                                gCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAAACTACGGATAACGGCCGCCACGGAAAAAACTTTCGGAGgccacaaaa                                                                 >  1:1091791/1‑232 (MQ=255)
                                                                                                                                                                                gCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATAAGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGAt                                                                 >  2:592474/1‑239 (MQ=255)
                                                                                                                                                                                     tttATACGCGAGGGCGCTTGCGGTAAAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑AAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAg                                                            <  2:904545/239‑1 (MQ=255)
                                                                                                                                                                                          aCGGGAGGGCGCTTGGGGTCCAACAATAACCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGTTAATCATCAACGGTTCGTTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGa                                                       <  2:867160/239‑1 (MQ=255)
                                                                                                                                                                                            gggggggCGGTTGCGGTAAAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGAcc                                                     <  2:859803/235‑1 (MQ=255)
                                                                                                                                                                                              ggggggctttGCGGAAAAACCATAAACCA‑GGAAGGGAAAGGCCGATGAC‑AAACCCA‑CCAACAATCGATGAAGTTGA‑AAGAAAGTTGGTTAACAGGGTAACCAACAACGGTTCGTTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCa                                                    <  1:263882/230‑1 (MQ=255)
                                                                                                                                                                                              gAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGTTAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCAt                                                   >  2:1004233/1‑239 (MQ=255)
                                                                                                                                                                                              gAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAACCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGAAAAAACTTTCGGATGCGCCAAATACAAGCCCAACCaa                                                   >  1:1237739/1‑238 (MQ=255)
                                                                                                                                                                                                tgtttaaaaaaaaaaaaCCATAAACCA‑GGAAGGGAAAGCCCGAAGAA‑AAAGCCA‑CCAAACATCGATGAAGCTGA‑AAAAAAGGTGGTTAACAGGGTAATCTTAAAGGTTTCGTTTAAACCTAACGGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGACCATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCa                                                    <  1:954897/221‑1 (MQ=255)
                                                                                                                                                                                                gggCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCC‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATAAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCAtt                                                  <  2:928951/238‑1 (MQ=255)
                                                                                                                                                                                                 ggCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATACCGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGa                                                >  2:1229716/1‑239 (MQ=255)
                                                                                                                                                                                                   gtaaaagggacagaCCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACATTCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAAt                                              <  2:254042/226‑1 (MQ=255)
                                                                                                                                                                                                     gtttgggttaaaacATAATCCAGGGAGAGGAAAG‑CCGATGGA‑TAACGTACCCAAAAACGGTTGAAG‑TGTAAAAAAAGTGGTTTAAGAGGGTAATCATAAGTGGTTGGCTGAAACTAAACGGGAGATTTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAAt                                              <  1:24834/225‑1 (MQ=255)
                                                                                                                                                                                                      ttGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCAGTGCGGTATAAGCCGAGATCCCCGGGAACATAGGGATAACGGCCGCCACGGTAAAAACCTTCGGATGCGCCAGAAACAAGCCCGACCATTAAAAAcc                                           >  1:166316/1‑239 (MQ=255)
                                                                                                                                                                                                        tgggTAAAACCAAAATACA‑GGAATGAAAAGGCCGATGGT‑TACCGCC‑CCACAAATTGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCAGCAACGGTTCGATGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCaa                                         <  1:178526/237‑1 (MQ=255)
                                                                                                                                                                                                        tggagaaaacccaaaTCCA‑GGATGGAAAACAGCGGTGGA‑AAACACA‑CAAAAAAGCGATAAAGGGGA‑AAAAAAGTTGGTTAACGGGGTAATCATAAACGGTTCGCTGTAAACTAGCTGGCTAATTTTTCCCGCCGAAATCAGTGCGGTATGAGCCGATATGGCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCaa                                         <  1:1004233/226‑1 (MQ=255)
                                                                                                                                                                                                        gCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAAAACTTCCGAATGCGCCAGATACCAACGCGCCCATTTGATACCaa                                         >  2:602019/1‑239 (MQ=255)
                                                                                                                                                                                                                  caaaaTCCA‑GGAATGGAAAGACCGATGGA‑TAACGCC‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGTTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGAc                               <  2:1262098/236‑1 (MQ=255)
                                                                                                                                                                                                                  aaTAATCAG‑GGATTGAAAATACCGTGGAA‑AAACGCA‑CCAAAAACGGATGAAGCTGG‑AAGAAAGTGGGTTAACGGGGAAATCAACAACGGTTCGTTGTACCCTAACTGGCTGATTTGTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGAc                               <  1:386436/238‑1 (MQ=255)
                                                                                                                                                                                                                   ctaaaccaagaaaTGGAAAGACAGATGGA‑AACCGCC‑CAAAAAAGGGGTGAGGTTTA‑AAGAAATTGGTTTAAGAGGGTAATCATAAACGGTTCGCTTTACCCAACCGGGCTGATTTTTCCCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGAc                               <  1:64215/228‑1 (MQ=255)
                                                                                                                                                                                                                      aTCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATAATTGCGGAATAAGCCGCTATGCCCGGGACCAACAGGCAAACGCCCGCCACGGGAAAACCTTTCGGAGCCGCCAGATACCAGACCGACCATTACAAAACAATCGTAccagcaac                            >  1:1239167/1‑232 (MQ=255)
                                                                                                                                                                                                                      aTCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGAGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCCCGGTAAACACTTTCGGATGGGCCAGAAAACAGCGCGGACATGAAAAACAAAAGGGACCGAcagga                           >  1:488940/1‑235 (MQ=255)
                                                                                                                                                                                                                            ggAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCa                                          >  2:954897/1‑219 (MQ=255)
                                                                                                                                                                                                                            ggAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAAATACCAGCCGGACCATTGAAAACCAATGGTACCGACCAGCAAAGa                      >  1:1262098/1‑239 (MQ=255)
                                                                                                                                                                                                                             gAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAAGGGCCGCCCCGGTGAACACTTTCCGATGCGCCCGAAACCAGCCCGCCCACTCAAAACCAATGGGACCGACCCGCaaaaaa                     >  1:8291/1‑234 (MQ=255)
                                                                                                                                                                                                                             gAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCCGATACCAGCGCGACCATTGAATACCAATGGTACCGCCCAGCATAGaa                     >  2:1206872/1‑239 (MQ=255)
                                                                                                                                                                                                                             gAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGAc                               >  2:268599/1‑229 (MQ=255)
                                                                                                                                                                                                                              actGGAAACACCGATGGA‑TAACCCA‑CCAAAAATCGATGAAGGTAA‑AAGAAAGTTGGTTACCGGGGAAACCATCAACGGTTCCGTTTACCCTAACTGGTTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGa                      <  2:1101354/235‑1 (MQ=255)
                                                                                                                                                                                                                              aaTGGAAAGCCCGATGGA‑TACCGCA‑CCAAAAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAg                       <  2:1126122/236‑1 (MQ=255)
                                                                                                                                                                                                                                  gAAAGACCGATGGA‑TAACGCC‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGACCGCCAAGTAAAACACTTTCGCTTGCGCAAGAAACCACCGGGCCCATCGAATACCAAATAGCCCGACCAGCAAAAAACCCaa                >  1:1222804/1‑238 (MQ=255)
                                                                                                                                                                                                                                  gAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCgg                                                                                                                   <  2:848258/140‑1 (MQ=255)
                                                                                                                                                                                                                                  gAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGATTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCCCCGAAATCATTGCGGAATAAGCCGATATGCCCGGGAACATAGGGATAACGCCCGCCACGGTAAACACTTTCGGACGCCCCAAATAAAACCGCGACCATTGAAAAACAAAAGTACAGACCAAAAGAGAAGCCAt                >  1:19436/1‑239 (MQ=255)
                                                                                                                                                                                                                                       aCCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGCAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGAGCGACCATTGAATACCAATGGTACCGACCAGCATAGAAGCCCTCAAgg           >  2:173863/1‑239 (MQ=255)
                                                                                                                                                                                                                                           aTGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGAAGCCATAAAGGTTg        >  2:816943/1‑238 (MQ=255)
                                                                                                                                                                                                                                            tGGA‑AAACGCA‑CCAACAATCGATAAAGCTGA‑AAGAAAGTTGGTTAACGGGTTAACCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGAAGCCATAAAGGTTGa       <  2:1240192/238‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                
TGCCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACAC‑GGAGGAGAGGAGGCTGTCTCTCATCCGTGTGCTTAAGCGAGGCAATTTTATACGCGAGGGCGCTTGCGGTACAACCATAATCCA‑GGAATGGAAAGACCGATGGA‑TAACGCA‑CCAACAATCGATGAAGCTGT‑AAGAAAGTTGGTTAACAGGGTAATCATCAACGGTTCGCTGTAACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGAAGCCATAAAGGTTGACCACT  >  NC_000913/4601444‑4601920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A