Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F111 I2 R1	11	49.6	1720206	93.9%	1615273	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,020,956	A→G	intergenic (‑37/‑182)	sulA ← / → sxy	SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,020,956	0	A	G	100.0%	116.6 / NA	44	intergenic (‑37/‑182)	sulA/sxy	SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/21); total (23/21)

CACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGAT  >  NC_000913/1020817‑1021089

cACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATgtgcag                                                                                                                                    <  2:303107/143‑1 (MQ=255)

   tGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATgtgcagtac                                                                                                                                 >  1:354305/1‑143 (MQ=255)

   tGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATgtgcagtac                                                                                                                                 >  1:289881/1‑143 (MQ=255)

         gCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACCTAATCAATCAAACCCCTGGGAGATACTGGATGCATCTGCAGTACAGCCaa                                                                                                                           >  2:336633/1‑142 (MQ=255)

         gCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGGACATAATAAATCCAGCCCCTCTGAGCTACTGGATGGATGAGCAGTACATcccc                                                                                                                           >  2:336520/1‑141 (MQ=255)

                  tAGTGGTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGAcaacaa                                                                                                                  <  2:781264/143‑1 (MQ=255)

                    gtgtTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAg                                                                                                                <  2:326471/143‑1 (MQ=255)

                      gtTTGCCGTAGAGACACGCGCAATTTCACTTGCTGCGGATGAGGACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGAt                                                                                                              <  1:195983/143‑1 (MQ=255)

                         ttCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCaa                                                                                                           <  1:235119/143‑1 (MQ=255)

                              tAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATTATCATTCCCGCCCCTCTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCATa                                                                                                      >  1:195994/1‑143 (MQ=255)

                               agagACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAATACATCCAGTGACAACACAGATCAACCCCAt                                                                                                     >  1:149226/1‑143 (MQ=255)

                                gagaCACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTAtt                                                                                                    <  2:79378/143‑1 (MQ=255)

                                   acacGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTc                                                                                                 >  1:256368/1‑143 (MQ=255)

                                   acacGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGACTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATCTTc                                                                                                 >  1:701074/1‑143 (MQ=255)

                                    gacGCGCAACTTTGCTGGGTGCGGAGGAGAACGACGAAGAACGATGGGCATAGCCTGAAGTGTACATAAGCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCg                                                                                                <  2:58733/142‑1 (MQ=255)

                                             ttttACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGCTCAACCCTATTATCGGAAagaggc                                                                                       >  2:201260/1‑141 (MQ=255)

                                               ttACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTTACAACAAAGATCAACCCTATTTTCGTAAAGAGCCTc                                                                                     >  1:135550/1‑143 (MQ=255)

                                                   ttGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGACAGAGCCTCGCaa                                                                                 >  1:732858/1‑143 (MQ=255)

                                                       tgcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAGttt                                                                             >  1:742853/1‑143 (MQ=255)

                                                        gtgggtGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAAtttt                                                                            <  2:306780/138‑1 (MQ=255)

                                                        gcGGATGAGCACGACGAAGAACGATGTGCATAGCCTCAAGTGTACATAATCAATCTAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGCTCAACCCTATTTTCGGAAAGAGCCTCGCAAAtttt                                                                            <  2:224001/143‑1 (MQ=255)

                                                          ggATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATCATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGt                                                                          <  2:446858/143‑1 (MQ=255)

                                                          ggATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGCCATACACGATCTGCCCTATTTTCGGAAAGAGCCTCGCAAATTTTGt                                                                          <  1:163266/143‑1 (MQ=255)

                                                           gATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTCGCGGAAAGAACCTCGCaagcattgtc                                                                         >  2:282482/1‑135 (MQ=255)

                                                               agaACGACGAAGAACGAGGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTAACAACCAAGATCAACCCTATCTTCTGAAAGAGCCTCGCAAATTTTGTCGTTg                                                                     <  1:364711/143‑1 (MQ=255)

                                                                gaACGACGAAGAACGATGTGCATAGCCTGGAGTGTACATAAGCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTgg                                                                    <  2:582297/143‑1 (MQ=255)

                                                                gaACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTgg                                                                    >  1:559705/1‑143 (MQ=255)

                                                                aaaCGAAGAAATACGTGGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGCCAACAAAGATCAACCCTATTTTCGGAACGAGCCTCGCAAATTTTGTCgccgt                                                                    >  2:841296/2‑139 (MQ=255)

                                                                       gaagaaCGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTAAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAGGAGCCTCGCAAACTTTGTCGTTGGTGatgat                                                             >  1:268863/1‑139 (MQ=255)

                                                                                    cATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATATTCGGAAAGAGCCTCGCAAAATATATCGTTGATGACGGGAAAACATAAATTa                                                >  2:128426/1‑143 (MQ=255)

                                                                                      tAGCCTGAGGGGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTGCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAAt                                              <  2:535645/143‑1 (MQ=255)

                                                                                            gAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTGTGTCGTTGGTGACGCGACAACCTAAATCAATCTTGcc                                        >  2:505373/1‑143 (MQ=255)

                                                                                                  tACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGGGCAGTACATCCAGTGACAACGAAGATGAACCCTATTTTCGGAAAGAGCCGCGCAAGATGTGGCGGTGGTGACGGGAGAACGTAAATTAATCTTGCCGCttga                                  >  2:605286/1‑141 (MQ=255)

                                                                                                    gataagtcgtcCAGGCCCGGTGAGGTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCATCCCGATTCTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGa                                <  2:747369/134‑1 (MQ=255)

                                                                                                    cATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCGAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGa                                >  1:140363/1‑143 (MQ=255)

                                                                                                       aatcaatcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATa                             <  1:436191/143‑1 (MQ=255)

                                                                                                       aatcaatcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGCTGGTGACGGGAAAACACCAATTAATCTTGCCCCTTAAGAATa                             >  2:252504/1‑143 (MQ=255)

                                                                                                         tcaatcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATCAg                           >  1:467540/1‑143 (MQ=255)

                                                                                                           gatcCAGCCGGTGGGAGTTACTGTGTGGATGTGCAGTACATCCAGTGCCAAGAAAGATCAGCCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAGTTAAGCTCGCCCCTTAAGAATAAGtt                         <  2:105983/142‑1 (MQ=255)

                                                                                                             ttcAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAAAAAAGATCAAACATATTTTCGGAAAGAGCCTCGAAAATTTTGTAGTTGCTGATGGGAAAAAATAAATTAATATAGAACGTTAAGAATAAGtaaa                       >  2:276242/3‑140 (MQ=255)

                                                                                                                     gtgagAGCTACTGTAGGGATGTGCGGTACATCCAGTGACAACAAAGATGAACCCTATGTTCGGAAAGAGCCTCGCAAAGTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTc               <  2:701074/139‑1 (MQ=255)

                                                                                                                     cTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTc               <  2:256368/143‑1 (MQ=255)

                                                                                                                        tgAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAAACCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTa            <  1:850156/143‑1 (MQ=255)

                                                                                                                            ttACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTa        >  1:457409/1‑143 (MQ=255)

                                                                                                                            ttACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTa        <  1:419686/143‑1 (MQ=255)

                                                                                                                                tGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGCAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCTTAGTTAACgg    >  1:776669/1‑143 (MQ=255)

                                                                                                                                  gaTGGACGTGCAGTACATCATGTGACAGCATAGCTCACCCTTATTTTCTGAAATAGCCTCGCAATTTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGAt  <  2:412332/142‑1 (MQ=255)

CACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGAT  >  NC_000913/1020817‑1021089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A