Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F9 I0 R1	260	102.5	2178490	93.2%	2030352	226.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	435,554	T→G	13.7%	*140G (TGA→GGA)	nusB →	transcription antitermination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	435,554	0	T	G	13.7%	336.2 / 7.8	139	*140G (TGA→GGA)	nusB	transcription antitermination protein
Reads supporting (aligned to +/- strand): ref base T (50/60); new base G (19/6); total (72/67)
Fisher's exact test for biased strand distribution p-value = 7.40e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

TACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTA  >  NC_000913/435317‑435790

tACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAG‑AAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGAAGAACCTGTGATTCGCCCTAAAAAAAAGGGa                                                                                                                                                                                                                                            >  2:688709/1‑239 (MQ=255)

ggtataagggacaggatgaAGCCATACCTGCCCCGCCGGCTGGAAGAACTGGGACAGGGAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTg                                                                                                                                                                                                                                             <  2:770059/225‑1 (MQ=255)

      gACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCCGAAGACAGCCATAAGTTCGTCAACGGCGGACCCGCTAAAGCCGCACCTGAGATTCGCCCTAACAAAAAAGAATATcc                                                                                                                                                                                                                                       >  2:1057398/1‑239 (MQ=255)

         ggACGGAGGAACCCAATCCTTGCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAgg                                                                                                                                                                                                                                    <  2:706533/239‑1 (MQ=255)

                     ccATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCCTACAAAGTGGCCATTAACGAAGCGATCGACCTGTCGAAATCGTTCGGCGACGAAGACACCCATAAGTTCGTCACCGGCGTCCTCGATAAAGCACCACCTGTAATTCACCCCAACAAAAAGGGTTATCCAGCCCGCAAGAttta                                                                                                                                                                                                                        >  2:947530/1‑237 (MQ=255)

                     ccATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGTCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGTCCGCCATAAGTTCGTCAACGGCGACCCCCATACAGCAACACCCGTGATTTGCCCTAAAAAAAAGGGTAATCAAGGACGGGAGATTCa                                                                                                                                                                                                                        >  2:5217/1‑239 (MQ=255)

                       ctcccatgcccGCCTGCGGGAAAAAGGGGAACGGGAAGAAAAAGCAGCACGGCGCATGGCGCTGTACCAACTGTCTAAACGTAGCCAATGT‑CCATACAAAGTGGCCATTAACGAAGCGATCGAACGGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACg                                                                                                                                                                                                                      <  2:945089/231‑1 (MQ=255)

                         aCCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGc                                                                                                                                                                                                                                   >  1:770059/1‑224 (MQ=255)

                         aCCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGAAAAACCAGCACCTGGGATCCGCCCTACCAAAAAGAGAAAGCCAGGCCGGGAGATTAACGGa                                                                                                                                                                                                                    >  2:490942/1‑239 (MQ=255)

                            tgccccGCCTGCTGGAAGAACGGGGACAGGTAAAAAAAGCAGTACTGCACATGGCGCTGTACGAACTGTCGAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGa                                                                                                                                                                                                                 <  2:790590/236‑1 (MQ=255)

                            tGTACCGCCTGGTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGATGTACGAACTGTCTAACCGTAG‑CGATGGGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGa                                                                                                                                                                                                                 <  1:1025065/239‑1 (MQ=255)

                             gTCCCGCCTGCGGGAAGAACTTGGACCAGTAAGAAAAGCAGTCATGCCGTTTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGAc                                                                                                                                                                                                                <  2:475305/239‑1 (MQ=255)

                               cccGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGCAAGCCCTAAGTTCGTCAACGCCGTCCTCGAACAAGCAGCACCTGTGATTCGCCCTAACAAAAAGGGAAACCCAGGCCGGTAGATTCAAGGAAGAc                                                                                                                                                                                                                >  2:774539/1‑237 (MQ=255)

                                   ccTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTGCTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTcc                                                                                                                                                                                                          >  1:240744/1‑239 (MQ=255)

                                   ccTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCCCCTGTGATTTGCCCTAACAAAAAGGGATCTCCAGGCCGGCAGATTCACGGAAGACCCTTcc                                                                                                                                                                                                          >  2:4561/1‑239 (MQ=255)

                                        tgGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGGGATATCCAGGCCGGGAGATTCACGGCAGACCGTTCCAAGa                                                                                                                                                                                                      >  2:797223/1‑238 (MQ=255)

                                            agaaCTGGGACAGGTAAAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGc                                                                                                                                                                                                  <  1:805465/238‑1 (MQ=255)

                                                    gACAGGTAAAAAAAGCAGGACTGGCCATTGGCGTGTACGAACTTTCTAAAGGTAG‑GGATGTGCCATACAAAGTGGCCATCAACGAAGGGATCGAACTGGCCAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCcttt                                                                                                                                                                                          <  2:240922/238‑1 (MQ=255)

                                                        ggTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCcttttcttt                                                                                                                                                                                     >  1:641066/1‑239 (MQ=255)

                                                        ggTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCAGTATCCCCGGCcttttcttt                                                                                                                                                                                     >  2:855363/1‑239 (MQ=255)

                                                         gTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAACCGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCGACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTGGATTCACGGAAGACCGTTCCATGATCGCCGGCattttctttt                                                                                                                                                                                    >  1:812962/1‑239 (MQ=255)

                                                         gTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATATAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCATGGCGGGTAGATTCACGGAAGACCTTTCCAGGATCGCCTGCcttttctttt                                                                                                                                                                                    >  1:231202/1‑239 (MQ=255)

                                                             aaaaaGCAGTACGGCGCATTGCGCTGTACGAACTGTCTAACCGTAG‑CGATGTGCCATACGAAGTGGCCAGTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTAc                                                                                                                                                                                <  1:828375/239‑1 (MQ=255)

                                                                aaggggttctggccATTGCGCTTGACGAAATGGCTAAACGTAG‑GGATGTGCCATACAAAGTGGCCATTAACGAAGCCATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACctg                                                                                                                                                                             <  2:577112/226‑1 (MQ=255)

                                                                 aGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACG‑AG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACct                                                                                                                                                                              >  2:218638/1‑236 (MQ=255)

                                                                   cAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAGAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACctgctc                                                                                                                                                                          >  1:574933/1‑238 (MQ=255)

                                                                   cAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAATGGAATTCCAGGCCGGTAAATTCCCGGAAGCCCGTTCCATGAACGCCGCCCTTTTCTTTTTTACcttctg                                                                                                                                                                          >  2:972048/1‑239 (MQ=255)

                                                                      tACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAACAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATAGCCGGCCTTTTCTTTTTTACctgctggc                                                                                                                                                                        >  1:654539/1‑236 (MQ=255)

                                                                       tcttgccATTGGGCTGTACAAATTTTCAAAACGGAG‑GGATGTGCCATTAAAAGTGGCCATTAACGAAGGGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGc                                                                                                                                                                      <  2:406621/233‑1 (MQ=255)

                                                                        cttggaaTTGCGCTGTACGAACTGTCTAATCGTAG‑CAATGTGCAATACAAAGTTGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCATTTTCTTTTTTACCTGCTGAGGCa                                                                                                                                                                     <  1:616055/233‑1 (MQ=255)

                                                                        ctggccATTGCGGTGTACGAACTGTCTAAACGTAG‑GGATGTGCCATACAAAGGGGCCATTAACGAAGGGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCa                                                                                                                                                                     <  2:22540/234‑1 (MQ=255)

                                                                         tGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACGGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCAt                                                                                                                                                                    <  1:879357/239‑1 (MQ=255)

                                                                          gcggATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATa                                                                                                                                                                   <  1:401368/239‑1 (MQ=255)

                                                                          gcgcATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAATAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATa                                                                                                                                                                   <  1:1029820/239‑1 (MQ=255)

                                                                          gcgcATAGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATAACAAGTGGCCATTAACGAAGCGATCGAACGGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATa                                                                                                                                                                   <  1:774539/239‑1 (MQ=255)

                                                                           cgcATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGGGATATCCAGGCCGGAAGATTCACGGAAGCCCGTTCCTTGATCGCCGGCCTTTTCTTTTTTCCCTCCTAAGGCATaa                                                                                                                                                                  >  1:485340/1‑239 (MQ=255)

                                                                           cgcATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGAGATATCCAGGCCGGTAGATTCACGTAAGACCGTTCCATGATCGACGGCCTTTTCTTTTTTACCTGCTGAGGCCTa                                                                                                                                                                   >  1:1040704/1‑238 (MQ=255)

                                                                              aTTGCGCTGTCCGAACTGTCTAAACGGTG‑CGATGTGCCATAAAAAGGGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTGCGGCGCAGAAGCCAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGt                                                                                                                                                               <  2:849202/239‑1 (MQ=255)

                                                                               ttGCGTGGTAGGAAATGTCAAAAGGTAG‑AGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGt                                                                                                                                                               <  2:372379/238‑1 (MQ=255)

                                                                                tGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTAt                                                                                                                                                             >  1:34717/1‑239 (MQ=255)

                                                                                tGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGGGATATCCAGGCCGGTAAATTCCCGGAAGACCGTTCCATCATCGCAGGCCTTTTCTTTTTTACCTGCTGAGCCAAAACGTaa                                                                                                                                                             >  2:879357/1‑238 (MQ=255)

                                                                                  cgcTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACgctgt                                                                                                                                                            >  1:729708/1‑234 (MQ=255)

                                                                                       tACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCAtgt                                                                                                                                                      >  1:73468/1‑239 (MQ=255)

                                                                                       tACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGAAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTAACTGCTGAGGCAAAACGTAGGGccggt                                                                                                                                                      >  2:655499/1‑235 (MQ=255)

                                                                                       tACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGGGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGCATGGCAtgt                                                                                                                                                      >  1:774577/1‑239 (MQ=255)

                                                                                            acggTCTAAACGGTG‑CGATGTGCCATACAAAGGGGCCATTAACGAAGGGACCGAACTGGTGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCg                                                                                                                                                  <  1:914789/235‑1 (MQ=255)

                                                                                              ctggcgaaacgtaagaagggcccAAACAAAGTGCCCATTAACGAAGCTGTCGAACTGGCGAAATCGTGCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAg                                                                                                                                                <  2:574933/218‑1 (MQ=255)

                                                                                                 cTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCTTCATCGCCGGCCTTTTCTTTTTTACACGCTGCGGCATAACGAATGGCAAGGGGGGGGTtc                                                                                                                                             >  2:110913/1‑238 (MQ=255)

                                                                                                 cTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGc                                                                                                                                                                      <  2:338306/213‑1 (MQ=255)

                                                                                                     aCGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGCCCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGGGTtttc                                                                                                                                           >  1:110585/1‑236 (MQ=255)

                                                                                                          g‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCCTAACg                                                                                                                                                                >  1:295021/1‑210 (MQ=255)

                                                                                                          g‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGAtt                                                                                                                                    >  1:35611/1‑238 (MQ=255)

                                                                                                          g‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCAAGGCCGGTAGATTCACGGAAGACCGTTCCATGATAGCCGGCCTTTTC‑TTTTTACCGGCTGGGGCATAACGTATGGCATGGGGCGGGGTTTCCCTGGTTg                                                                                                                                   >  2:1073418/1‑238 (MQ=255)

                                                                                                          g‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGGGATATCCAGGCCGGGAGATTCCCGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCAAAACGCATGGCATGTGGCGAGTTTTCCCTGttt                                                                                                                                    >  2:363077/1‑238 (MQ=255)

                                                                                                          g‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGGGATTCGCCCTAACAAAAAGGGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGAATGGCATGGGGGGAGTTCTCCCCGAtta                                                                                                                                   >  2:625546/1‑238 (MQ=255)

                                                                                                          g‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATAGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCCACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTTTTTTTAACCTGCTTCGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGAtt                                                                                                                                    >  1:500542/1‑238 (MQ=255)

                                                                                                          acggATTTGCCATACAAAGTGGCCCTTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGAtt                                                                                                                                    <  2:240744/236‑1 (MQ=255)

                                                                                                                tgtccATAAAAGGGGGCCTTTAACGAGGCGTTCCAACCGGCGAAATCGTTCGGGGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGt                                                                                                                              <  2:35611/236‑1 (MQ=255)

                                                                                                                 tgCCATAAAAAGTGGCATTTAACGAAGCGATCGAACTGGCAAAATCGTTCGGCGCAGAAGCCAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGccc                                                                                                                                <  2:814791/236‑1 (MQ=255)

                                                                                                                      tACAAAGTGGCCATTAACGAAGCGATCGAAATGGCGAAATCGTTCGGCGCAGAAAACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACAGGTGATTCGCCCTAACAAACACGGATATCCAGGCCGGAAAATTCACGAAATACCGTTCCATTATTGACGTCCTTTTCTTTTTTACCTGCTTATACCTAACGTAAGACTTGTTGCCATGTTTCCCAGATTgacatgtttttt                                                                                                                        >  2:654719/1‑228 (MQ=255)

                                                                                                                           aGTGGCCATTAACGAAGCGATTGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGCCCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATACCGTATGGAATGTGGCGAGTTCTCCCTGATTGCCCGTTAtttttcccg                                                                                                                   >  1:696266/1‑234 (MQ=255)

                                                                                                                             tGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGAAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGAcgggg                                                                                                                 >  2:714845/1‑235 (MQ=255)

                                                                                                                                ccATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGGGTaa                                                                                                              >  1:940430/1‑239 (MQ=255)

                                                                                                                                ccATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCCCCGGCCTTTTCTTTTTTACCTGCTGAGGCTTAACGTATGGCCTGTGCCGAGTTTTCCCTGATTGCCCGTAATTTTGACCGGGTaa                                                                                                              >  2:59969/1‑239 (MQ=255)

                                                                                                                                    tAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTaagaag                                                                                                          <  2:970277/239‑1 (MQ=255)

                                                                                                                                     aaCGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCAt                                                                                                                                                                    >  1:573262/1‑180 (MQ=255)

                                                                                                                                          aGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGGCTGTGGCGGGTTTCCCCTGATTGCCCGTTCTTTTTACCGTGTAAGAAGTtctgg                                                                                                    >  2:823123/1‑237 (MQ=255)

                                                                                                                                              aTCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTTTTTTTACCGTGTAAAAAGTTCTCGTCtt                                                                                                >  2:226409/1‑239 (MQ=255)

                                                                                                                                                       gCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGGAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTCCTTAGGCATAACGTATGGCATTGGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGGCGAAGAAGTTCTCGTCTTGATGTAGaa                                                                                       >  2:733038/1‑239 (MQ=255)

                                                                                                                                                           aagcGTTAGGCGCAGAAGACAGCCATAAGTTCGGCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTg                                                                                    <  1:1063804/235‑1 (MQ=255)

                                                                                                                                                              cGTTCGGCGCAGAAGCCAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCa                                                                                <  1:562332/239‑1 (MQ=255)

                                                                                                                                                                 tCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGt                                                                                                                                                                                                                               <  1:159845/93‑1 (MQ=255)

                                                                                                                                                                 tCGGCGAAGAAGACAGCCATAAGTTCGTCAACGGCGTAATCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGt                                                                                                                                                                                                                               >  2:159845/1‑93 (MQ=255)

                                                                                                                                                                      gcAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGcga                                                                         <  1:4561/238‑1 (MQ=255)

                                                                                                                                                                          aagaCAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGAtt                                                                    >  2:345392/1‑239 (MQ=255)

                                                                                                                                                                             cccaccctAAGTTCGTCACAGGGGTACTCGATAAAGCAGCCCCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTgcg                                                                 <  2:774577/232‑1 (MQ=255)

                                                                                                                                                                             aCAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTgcg                                                                 <  1:855363/239‑1 (MQ=255)

                                                                                                                                                                               aGCCATAGGTTGGGCAAGGGCGTACGCGAAAAAGGCGCCCCTGGGATTCGCCCTAACAAAAAGTGATTTTCAGGCCGGTAGATTCACGGAAGCCCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTgcg                                                                 <  1:954541/237‑1 (MQ=255)

                                                                                                                                                                               aGCCATAAGTTCGCAAACGTGGTCCTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGTCCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTgcg                                                                 <  1:972048/237‑1 (MQ=255)

                                                                                                                                                                                  caaaattccgccAACGGGTTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACtt                                                            <  2:110585/228‑1 (MQ=255)

                                                                                                                                                                                  cATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCCCCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCGCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCa                                                               <  1:51605/236‑1 (MQ=255)

                                                                                                                                                                                  cATAAATTCGGAAACGGCGTACTCGATAAATCTGCACCTGTGATTCGCCCTAACAAAAAGTGATAGCCAGGCCGGTAGATTCACGGAAGGCCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGTTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGGCCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGTCGACGATTgcg                                                                 <  1:393752/234‑1 (MQ=255)

                                                                                                                                                                                    tAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATa                                                                                                                                                                   <  2:719665/134‑1 (MQ=255)

                                                                                                                                                                                           gTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTGAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGTTTGGGACCTTCTCataat                                                    >  1:495621/1‑234 (MQ=255)

                                                                                                                                                                                                                  agcaCCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATg                                                                                            >  1:314522/1‑175 (MQ=255)

                                                                                                                                                                                                                   gcaCCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGAt                            <  1:218638/238‑1 (MQ=255)

                                                                                                                                                                                                                   gcaCCGTTGTTTTGCCCTAACAAAAAGTGGTTCCCAGCCCCGTAGGTTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGAt                            <  2:121323/238‑1 (MQ=255)

                                                                                                                                                                                                                    caCCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCa                          >  1:219714/1‑239 (MQ=255)

                                                                                                                                                                                                                    caCCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCa                          >  2:45678/1‑239 (MQ=255)

                                                                                                                                                                                                                    caCCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAAAAGAACCTGGCggtag                          >  2:338943/1‑235 (MQ=255)

                                                                                                                                                                                                                     aCCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGccc                                                                                                                                >  1:485118/1‑136 (MQ=255)

                                                                                                                                                                                                                     aCCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAg                         <  1:655499/239‑1 (MQ=255)

                                                                                                                                                                                                                      ccTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGAACCTGGCGATCAg                         >  1:557752/1‑238 (MQ=255)

                                                                                                                                                                                                                       ctttgtttCCCCTTAAAAAAAAGGGATACCCAGGCCGGTAAATTGCCGGAAGAACGTTCCATGATCGCCGGCTTTTGCGTTTTGACCGGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTATTGATGGCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCa                       <  2:812265/233‑1 (MQ=255)

                                                                                                                                                                                                                           ggttCGCCCTAACAAAAAGGTATTTCCAGGCCGGTAGATTCCGGGAAGGCCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTTCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGa                   <  2:73468/237‑1 (MQ=255)

                                                                                                                                                                                                                               cGCCCTAACAAAAGGTGATATCCAGGGCGGGTGATTCAAGGAAGGCCGTTCCATGATCGCCGGCCTTTTCTTGTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACCGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACg               <  2:419376/239‑1 (MQ=255)

                                                                                                                                                                                                                                 cccTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTgcg                                                                 <  1:575704/187‑1 (MQ=255)

                                                                                                                                                                                                                                 cccTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTgcg                                                                 >  2:575704/1‑187 (MQ=255)

                                                                                                                                                                                                                                    tAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCtggt          <  1:345392/239‑1 (MQ=255)

                                                                                                                                                                                                                                            aGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGCCGATCAGCACTGATACGCTGGTGGCGGGTa  >  1:309896/1‑239 (MQ=255)

TACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAG‑CGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTA  >  NC_000913/435317‑435790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A