Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F9 I0 R1 260 102.5 2178490 93.2% 2030352 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 761,097 A→C 16.2% T798P (ACC→CCC)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913761,0970AC16.2% 219.4 / 2.1 111T798P (ACC→CCC) sucA2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base A (32/54);  new base C (19/5);  total (51/60)
Fisher's exact test for biased strand distribution p-value = 3.81e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

CGCACGGTTACGAAGGGCAGGGGCCGGAGCACTCCTCCGCGCGTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCTCACGTCAAGGATTTTGTCTGGTGCCAGGAAGAGCCGC  >  NC_000913/760861‑761334
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
           gAAGGGCGGGGGCGGGGGCCCTCCTCCGGGCGTCTGGAACGTTAGCTTCAACTTTGTGCGGAGCAAAACATGCAGGTTTGCGTACCGTCTCCCCCGGCACGGGGTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑gccagc                                                                                                                                                                                                                                   <  2:314019/239‑1 (MQ=255)
               ggCAGGGGCCGGAGCACTCCTCCGCGCGTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACAACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCTCGATGTCGCCGAAATCCCTGCTCCGTCATCCCCTGGGGGTTTCCTCCCCCCCAGAACTGGCGAACGCACCCTTCCT‑gccagcc                                                                                                                                                                                                                                  >  2:766072/1‑236 (MQ=255)
                 cAGGGGCCGGAGCACTCCTCCGCGCGTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCCCCCGCTGGGGGGTTCCAGCCCCGAAAAAACGGCGAACGGACCCTTCCG‑gccagcccaggg                                                                                                                                                                                                                             >  2:490548/1‑234 (MQ=255)
                 cAGGGGCCGGAGCACTCCTCCGCGCGTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCgt                                                                                                                                                                                                                             >  1:660229/1‑238 (MQ=255)
                 cAGGGGCCGGAGCACTCCTCCGCGCGTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCCCGACGAACTGGCGACCGGCACCTCCCT‑gcccgcccccgg                                                                                                                                                                                                                             >  1:390137/1‑234 (MQ=255)
                 cAGGGGCCGGAGCACTCCTCCGCGCGTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGGCATCCCCTGGCGGGTTCCAGCCTCGAAGAAATGGCGAGCCGCCCCCTCCT‑GCCAACCACCgg                                                                                                                                                                                                                             >  2:895577/1‑239 (MQ=255)
                   ggggCCGGAGCACTCCTCCGCGCGTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGAGGCGTCGTCCGCTGGTCGTGCTGTCGCCGGAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAACCTGGAAGAACTGGCGAACGGCACCCTCCT‑gccagcccctgggg                                                                                                                                                                                                                           >  2:682447/1‑232 (MQ=255)
                      gCCGGAGCACTCCTCCGCGCGTCTGGAACGTTATCTGAAACTTTGGGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGaa                                                                                                                                                                                                                         <  1:128222/238‑1 (MQ=255)
                           gccACTCCCCCCGGCGTTTGGAACGTTATCTGCAACTTTGTTCGTGGCAAACCATGCCGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATcga                                                                                                                                                                                                                    <  2:26316/236‑1 (MQ=255)
                              aCTCCTCCGCGGGGTTGGAAGTTTATCTGAAACTTGTGGCTGAGCAAAACATGCAGGTTTGCTTCCCGTCTACCCCGGCACAGGTCTACCACATGCGGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGGCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATcgacga                                                                                                                                                                                                                 <  2:654813/238‑1 (MQ=255)
                                tcctccGCGCGTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCTAACGGACCCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCt                                                                                                                                                                                                              >  1:655605/1‑239 (MQ=255)
                                tcctccGCGCGTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGCCGAGCt                                                                                                                                                                                                              >  1:1039843/1‑239 (MQ=255)
                                tcctccGCGCGTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCCCTGGCGGTTTCCACCCCCGAAGAACTGCGGAACGGCCCCTTCCT‑CCCCCCCACCGGTGAAACcgccag                                                                                                                                                                                                                 >  2:337340/1‑232 (MQ=255)
                                       cgcgTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAGGAACTGGCGAACGGCCCCTTCCT‑GCCAGCCATCGGGGAAATCGACGGGCTTGATCCg                                                                                                                                                                                                       >  1:317188/1‑239 (MQ=255)
                                        gcgTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGa                                                                                                                                                                                                      <  1:228786/239‑1 (MQ=255)
                                        gcgTCTGGAACGTTATCTGCAACTTTGGGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGa                                                                                                                                                                                                      <  1:337340/239‑1 (MQ=255)
                                            cTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCCGCCTCGAAGAACTGGCGAACGGGCCCTTCCT‑GCCAGCCACCGGGGAAATCGACAGGCTTGACCCAA‑Aagg                                                                                                                                                                                                 >  2:253067/1‑239 (MQ=255)
                                            cTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCCCCGGTCGTGAAGTCCCCGAACTCCCCGCTGCGTCACCCCCTGGCGGTTTCCAGCCCCGAAGAAATGTCGAACCGCCCCTCCCT‑GCCTGCCCACGGGGaaaaacccgacccgaatccccaggg                                                                                                                                                                                                  >  2:140699/1‑217 (MQ=255)
                                                 aCGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTg                                                                                                                                                                                             <  1:164743/238‑1 (MQ=255)
                                                  cGTTTTCTGCCATTTGGGGCTGAGCAAAACATGCAGGTTGGCTGTCCGTCCACCCCGGCCCCGGTTTACCAAAGTGTGCGGCGGCAGGCGGTGGCGGGGAGGGGTCGTCCGCTGGTCGTGATGGCCCCGAAATCCCTGCTGCGTCCTCCGCTGGCGGTTTCCAGCCTAGAAGAACTGTCGAACGGCCCCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGaa                                                                                                                                                                                           <  2:573304/239‑1 (MQ=255)
                                                  cGTTATCTGCAACTTTGTGCTGAGCAAAACCTGCAGGTTTGCGTCCCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGa                                                                                                                                                                                            <  1:490548/238‑1 (MQ=255)
                                                     tttCTGCAATTTTGTGCGGAGCAAACCAGGCGGGTTGGCGTACCGTCTGCCCCGGCAAAGGTTGACCACAGGCGGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGGCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAgcg                                                                                                                                                                                        <  2:289402/237‑1 (MQ=255)
                                                      aTCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGCAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCCTCGGGGAAATCGACGAGCTTGATCCCA‑AAGGCGTgcaaggg                                                                                                                                                                                       >  2:629872/1‑233 (MQ=255)
                                                      aTCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCCCCGAAATCCCTGCGGCGTCATCCGCTGGCGGTTTCCAGGCCTGCGGAACTGGCCAACGGCCCCCTCCT‑GCCGCCCCTCGGGGGAATCGGGACGCTTGTTCCGC‑AGCGCGTGAcgcg                                                                                                                                                                                        >  2:231920/1‑238 (MQ=255)
                                                       ttgtacaCTTTGGGCTGAGCAAAACCGGCAGGTTTGGGTCCCGGCTCACCCGGCACGGGTTTACCCCATGCTGCGTCGGCAGGCGGTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGcgcg                                                                                                                                                                                      <  2:219135/233‑1 (MQ=255)
                                                       tCTGCAATCTTGGGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGGGGGATGCGCCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGcgcg                                                                                                                                                                                      <  1:397954/239‑1 (MQ=255)
                                                              cttttgtcTGGGCAAACCATGGCGGTTTGGGTCCCTTCTCCCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGGTGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGAt                                                                                                                                                                               <  2:94376/232‑1 (MQ=255)
                                                              cttttgggtggggaaaaCATGCGGGTTTGCGTCCCGGTTCCCCGGGCACAGGTTGACCCCGTGGTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGGCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGAt                                                                                                                                                                               <  2:562853/226‑1 (MQ=255)
                                                              ctttgggcgtgacAAAACATGCAGGTTTGCGTCCCGTTTACCCCGGGACAGGTTTACCACATGCGGGGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGAt                                                                                                                                                                               <  2:660229/227‑1 (MQ=255)
                                                              cTTTGTGCTGAGCAAAACAGGCAGGTTTGCGTTCCGTCTACCCCGGCACAGGTTTACCCCATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGAt                                                                                                                                                                               <  1:545501/239‑1 (MQ=255)
                                                              cTTTGTGCTGAGCAAAACAGGCAGGTTTGCGTACCGTCTGCCCGGGCCCGGGTTTACCACATGCTGCGTGCTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGAt                                                                                                                                                                               <  1:501836/239‑1 (MQ=255)
                                                                 tgggCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGAtgtg                                                                                                                                                                            <  1:895577/236‑1 (MQ=255)
                                                                   tgCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGGCGAAACCCCTGCTGCGTCGTCCGCTGGGGGTTTCCAGCCTCGGAGAACCGCGGAACGGACCCTTCCTCGCCA‑CCACCCGGGAACTCCACGAGCGTGTTCCGA‑AGGGCAGGAAgacggcggggaagtgtt                                                                                                                                                                          >  2:13638/1‑223 (MQ=255)
                                                                        aGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACCAGCTTGATCCGA‑AGGGCGGGAAGGGGGTAGGAATGGGGTCTGGt                                                                                                                                                                     >  1:3576/1‑239 (MQ=255)
                                                                        aGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCCCGAAGAACCGGCGAACGGCCCCTTCCA‑GCCAGCCCACGGGGAAATCGACGAGATTTATCCGC‑AGAGCCGGAAGCGCGtggaggggggttttgga                                                                                                                                                                     >  2:777942/1‑223 (MQ=255)
                                                                          cAAAACATGGGGGTTGGCGTCCCGTCTCCCCCGGCCCGGGTTTCCCACAGGCGGCGTCGGCAGGCGCGGGGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTa                                                                                                                                                                    <  2:827946/238‑1 (MQ=255)
                                                                             aaCATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCCCGAAGAACTGGCAGGCGGCACCTTCCT‑GCCAGCCCTCGGTGCAACCAACAGACATCATCCGA‑AGGGCGGGAACCGCGTAGAGATGTATTTTGGTAAggg                                                                                                                                                                >  2:364153/1‑238 (MQ=255)
                                                                             aaCATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGGTTCCAGCCTCGAAAAACTGGCGAACGGCCCCCTCCT‑GCCAGCCATCGGTGAAATCGACAGGCTGGAGCCGA‑AGGGCTGGAAGCGCGtgaggaagggtttggggaaggg                                                                                                                                                                >  2:183816/1‑218 (MQ=255)
                                                                                  gCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCCCGTAGTGATGGGTTCTGGTAAGGGttatt                                                                                                                                                           >  1:345678/1‑239 (MQ=255)
                                                                                  gCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGGTTCCAGCCTCGAAGAACTTGCGAACGGACCCTTCCT‑GCCAGCCATCGGGGAAATCGGCGCGCTTGATTCGA‑AAGGCGTGAAGGCGGGAGGGAAGGGTTCGGGGAAGGGttatt                                                                                                                                                           >  2:611290/1‑239 (MQ=255)
                                                                                       tttgcgctccgcttcccccGCCACGGGTTTCCCCCGTGCTCGGTCGTCAGGCGCTGCGGGGGATGCGTCGTCCGGTGGTCGTGATGACGCCGAAAGCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCCCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGa                                                                                                                                                       <  2:730133/223‑1 (MQ=255)
                                                                                       tttGCTTTCCGTCTTCCCCGGCCCAGGTTTACCACATGCTGCGTCGCCAGGCGCTGCGCGGGATGCGTCGGCCGCTGGGCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGa                                                                                                                                                       <  2:67169/238‑1 (MQ=255)
                                                                                       tttGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGa                                                                                                                                                       <  1:777942/238‑1 (MQ=255)
                                                                                       tttGCGTACCGGTTACCCCGGGACCGGTTTACCACATGCTGCCGCGTCAGGCGCTTCGCGGGATGCGTCGGCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGa                                                                                                                                                       <  1:400306/238‑1 (MQ=255)
                                                                                       tttGCGTACCCTCTACCCCGGCAAGGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGa                                                                                                                                                       <  1:766892/238‑1 (MQ=255)
                                                                                           cGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCCGCCTCGAAGAACTGGCGACCGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGCCGAGCTTGATCCGA‑AGGGCGTGCAGCGCGTAGGGAGGTGTTCTGGGAAGGGTTATTACGACctgg                                                                                                                                                  >  2:54265/1‑238 (MQ=255)
                                                                                           cGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGCAGAACCGGCGAACGGCCCCTTCCT‑GCCAGCCATCGGTGAAATCGACGGGCTTGAGCCGA‑ACGGGGGGAAGCGCGTAGTGGTGTGTTTGGGGAAGGGTTTTTACGaaccgc                                                                                                                                                  >  2:936175/1‑234 (MQ=255)
                                                                                                cGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGACCGGCCCCTTCCT‑GCCAGCCATCGGTGAAATCCACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGGGATGTGTTCTGGTAAGGTTTTTTACGCCCTCCTGGa                                                                                                                                              >  1:337079/1‑238 (MQ=255)
                                                                                                 gTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGGGAAATCGGCGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGGTGTGTTCTGGTAAGGTTTTTAAAGACAtggtgggaa                                                                                                                                            >  2:857764/1‑236 (MQ=255)
                                                                                                  tCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCCCCTTCCT‑GCCAGCCATCGGGGAAATCGACGAGCTTGATCCGA‑AGGGGCGGAAGCGCGTAGGGAGGTGTTCTGGGAAGGGTTTTAAAGACCGGCGGGaaaa                                                                                                                                           >  2:530949/1‑237 (MQ=255)
                                                                                                  tCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGAGCGTAGGGATGTGTTCTGTTAAGGTTTATTACGACCTGCTGGAACa                                                                                                                                           >  1:873458/1‑239 (MQ=255)
                                                                                                       cccGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑CGGGCGTGAAGGCCGCAGTGATGGGTTCTGGTAAGGTTTTTTAAGACCTGCTGGAACAGcgcc                                                                                                                                      >  2:177657/1‑237 (MQ=255)
                                                                                                          ggCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGCGAAACGCGAAGGGATGTGTTTTGGGAAGGTTTATTACGACCTGCTGGAACAGCGGCGTa                                                                                                                                   >  1:1027930/1‑239 (MQ=255)
                                                                                                            cacaGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCCGCCCTCGGTGAAATCGCCGAGCTTGATCCGA‑AGGGCGTGAAGCGCGGAGGGTGGGGTTCTGGGTAGGTTTATTAAGACCGTCTGGaaaaccggcggaaa                                                                                                                                 >  2:516753/1‑227 (MQ=255)
                                                                                                            cacaGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGAAAGGTTTATTACGACCTGCCGGAAAAGCGTCGTAAg                                                                                                                                 >  1:851522/1‑239 (MQ=255)
                                                                                                                 gTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACa                                                                                                                             >  1:622304/1‑238 (MQ=255)
                                                                                                                      ccACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGACGTTGATCCGA‑AGGGCGGGAAGGACGTAGTGATGGTTTTTGGTAAGGGTTTTTACGACCGCCTGGacaacggccgaaagaaaaacaaaa                                                                                                                       >  2:488586/1‑216 (MQ=255)
                                                                                                                       cacattgggggtccgcAGGCCCTGCGCGGGATTGTGCGCCCGCGGGTCCGGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAaca                                                                                                                      <  2:738680/224‑1 (MQ=255)
                                                                                                                           tgctgcGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGGGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGAt                                                                                                                  >  1:738680/1‑239 (MQ=255)
                                                                                                                           tgctgcGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCCA‑AGGGCCGGAAGCGCGTAGTGGTGTTTCCTGGTAAGGTTTATTACGACGTGCTGGAACAGGGCCGGAAGAAAAATCAacacctg                                                                                                                  >  1:715755/1‑236 (MQ=255)
                                                                                                                           tgctgcGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGACCCTTCCT‑GCCCGCCATCGGTGAAATCGACGGGCTTGATCCGAGAGGGGGT‑CAGCGCGTAGGGGTGTGTTCTGGGAAGGTTTATTACGACCCGCTGGAAAAGCGGAGTAAAACCAAACAAAACGAt                                                                                                                  >  2:1047827/1‑239 (MQ=255)
                                                                                                                             ctgcGGCGTAAGGCGCTGCGCGGGAGGCGTCGTCCGCTGGTCGTGATGTCCGCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACGTTCCT‑GCCAGCCATCGGTGAAAGCGACGAGCTTGATCCGA‑AGGGCGTGAAGAGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATg                                                                                                                 <  1:600367/238‑1 (MQ=255)
                                                                                                                                 gtcgtcAGGCGCTGCGCGGGATGCGTCGTCCGGTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGcc                                                                                                            <  1:458315/239‑1 (MQ=255)
                                                                                                                                      ccggcggtcgggggggTGCGTCGTCCGCTGTTAGTGATGTCGCCGAAATCCCTGCTTCGGCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATtgt                                                                                                       <  2:478694/227‑1 (MQ=255)
                                                                                                                                      cAGGCGCTGGGCGGGATGCGTCGGCCGCTGGGCGTGATGTCGCCGAAATCCCTGCTGCGTAATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATtgt                                                                                                       <  1:516753/239‑1 (MQ=255)
                                                                                                                                          cgcTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTTTGCGt                                                                                                   >  1:313512/1‑239 (MQ=255)
                                                                                                                                           cgttgggggggggggcggtcCGCGGGCGGTGATTGCGCCGAAATCCCTGCGGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCGTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTa                                                                                                  <  2:337079/222‑1 (MQ=255)
                                                                                                                                           cgtGCGGGGGTTGGCTCGTCCCGGGGTCGTGATGGCGCCAAACTCCCTGCTGCGTCATCCGCGGGCGTTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCAGCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTa                                                                                                  <  2:602791/237‑1 (MQ=255)
                                                                                                                                               cgcgGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGAATct                                                                                               >  1:638018/1‑237 (MQ=255)
                                                                                                                                                ggggggTGCCTCGGCCCCTGGTCGGGATGTCCCCGAAACCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAg                                                                                             <  2:1027930/237‑1 (MQ=255)
                                                                                                                                                gggggATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAg                                                                                             <  1:489086/237‑1 (MQ=255)
                                                                                                                                                gcgGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGa                                                                                              <  1:530949/238‑1 (MQ=255)
                                                                                                                                                 tgggTTGTGTGGTCCGCTGTTCGTGAAGTCGCCGAAATCCCTGCTGGGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCGCCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGc                                                                                            <  2:625270/238‑1 (MQ=255)
                                                                                                                                                           cgtcCGGTGGTCGTGGTGTCGCCGAAATCCCTGCTGCGTCATCCGGTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTAcc                                                                                   <  1:709165/238‑1 (MQ=255)
                                                                                                                                                              ccGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTCCGACCTGCTGGAACAGCGTCGTAAGAAAAATCAACACGATGTCGCCATTGTGCGTATCCAGCAACTCTACCCGt                                                                                >  1:414510/1‑238 (MQ=255)
                                                                                                                                                                  tGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCGGCTGGAACAGCGTCGTAAGAACAATCAACACGATTTCGCCATTGTGCGTATCGAGCAACCCTAACCGTTccc                                                                            >  1:285134/1‑238 (MQ=255)
                                                                                                                                                                    gTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCCCa                                                                         >  1:693499/1‑239 (MQ=255)
                                                                                                                                                                          ttttccccGAAATCCCTTGTGCGTCATCCGGGGGCGGTTTCCAGGCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGc                                                                   <  2:1039843/233‑1 (MQ=255)
                                                                                                                                                                          aTGTCGGCGAAATCCCTGCTGCGTCATCCGGTGGGGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGc                                                                   <  1:65043/239‑1 (MQ=255)
                                                                                                                                                                           tGTCGCCTAAATCCCTGCTGGGTCACCCGCTGGGGGTTTCCAGACTCGAAAATCTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGc                                                                   <  1:613878/238‑1 (MQ=255)
                                                                                                                                                                            gggtaaaagagacagggtgcGTCATCCGCGGGCGGTTTACAGCCTCGAAGAACTGGCGAGCGGCACCTTCCG‑GCCAGCAATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCg                                                                  <  1:498519/221‑1 (MQ=255)
                                                                                                                                                                                                 tCATCCGCTGGCGGTTTCCAGCCTCGCAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGGATCGAGACACTCTACCCGTTCCCGCATAAAGCGATGCAGGACGGGTTGCCACAGt                                            >  1:227766/1‑239 (MQ=255)
                                                                                                                                                                                                    tCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACACTCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGGGTGTCAGCAGttt                                          >  1:161290/1‑238 (MQ=255)
                                                                                                                                                                                                    tCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGGAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGAATCGGGCAACTCTACCCGTTCCCGCCTAAAACGAGCCGGGAAGGGTTGCACCAGttt                                          >  2:699777/1‑238 (MQ=255)
                                                                                                                                                                                                      cGCTGGGGGTTTCAAGGCTCGGAGAACTGGCGAACCGCACCTTCCT‑GCCAGGCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCt                                       <  1:920612/239‑1 (MQ=255)
                                                                                                                                                                                                      cGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCt                                       <  1:253067/239‑1 (MQ=255)
                                                                                                                                                                                                      cGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCt                                       <  1:952087/239‑1 (MQ=255)
                                                                                                                                                                                                         tGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGCCGTAAGAACAATCAACACGATGTCGCCCTTGTGCGTATCGAGCAACTCTACCCGTTCACGCATAAAGCGATGCCGGAAGTGTTGCAGCGGTTTGCTc                                      >  1:213877/1‑237 (MQ=255)
                                                                                                                                                                                                             ggTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCTCACGTCa                                <  2:313512/239‑1 (MQ=255)
                                                                                                                                                                                                              gTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCTCACGTCa                                <  1:610383/238‑1 (MQ=255)
                                                                                                                                                                                                               tgtCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTTATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCTCACGTCaa                               <  1:35053/236‑1 (MQ=255)
                                                                                                                                                                                                                   cAGCCGCGAAGAACGGGCGAACGGCACCTTCCT‑GCCAGCCCTCGGTGAAATCGACTATCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCTCACGTCAAGGAtt                          <  1:1047827/239‑1 (MQ=255)
                                                                                                                                                                                                                        tCGAAGAACTGGCGAACGGCCCCTTCCT‑GCCAGCCATCGGTGAAATCGGCGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCTCACGTCAAGGATTTTGTc                     <  1:907659/239‑1 (MQ=255)
                                                                                                                                                                                                                        tCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCTCACGTCAAGGATTTTGTc                     <  1:183816/239‑1 (MQ=255)
                                                                                                                                                                                                                        tCGAAGAACTGGCGAACGGCACCTTCCT‑GACAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCTCACGTCAAGGATTTTGTc                     <  1:699777/239‑1 (MQ=255)
                                                                                                                                                                                                                        agaggctgcTGGGGAACGGCCCCTTCCT‑GCCAGCCATGGGTGAAATCGACGAGCTTGAGCCGG‑AGGGCGTGAAGCGCGTAGTGATGTTTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCTCACGTCAAGGATTTTGTc                     <  2:479066/231‑1 (MQ=255)
                                                                                                                                                                                                                          gaagaaCTGGCGAACGGCCCCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCTCACGTCAAGGATTTTGTCTg                   <  1:629872/239‑1 (MQ=255)
                                                                                                                                                                                                                                       aCGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCTCACGTCAAGGATTTTGTCTGGTGCCAGGAaga       <  1:857764/238‑1 (MQ=255)
                                                                                                                                                                                                                                           cACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCTCACGTCAAGGATTTTGTCTGGTGCCAGGAAGAGCCg   <  1:611290/238‑1 (MQ=255)
                                                                                                                                                                                                                                           cACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGACCAACTCTACCCGTTCCCGCATAAACCGATGCAGGAAGGGTTGCAGCAGTTTGCTCACGGCAAGGAGTTTGTCTGGTGCAAGGactgtcact  >  1:479066/1‑231 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
CGCACGGTTACGAAGGGCAGGGGCCGGAGCACTCCTCCGCGCGTCTGGAACGTTATCTGCAACTTTGTGCTGAGCAAAACATGCAGGTTTGCGTACCGTCTACCCCGGCACAGGTTTACCACATGCTGCGTCGTCAGGCGCTGCGCGGGATGCGTCGTCCGCTGGTCGTGATGTCGCCGAAATCCCTGCTGCGTCATCCGCTGGCGGTTTCCAGCCTCGAAGAACTGGCGAACGGCACCTTCCT‑GCCAGCCATCGGTGAAATCGACGAGCTTGATCCGA‑AGGGCGTGAAGCGCGTAGTGATGTGTTCTGGTAAGGTTTATTACGACCTGCTGGAACAGCGTCGTAAGAACAATCAACACGATGTCGCCATTGTGCGTATCGAGCAACTCTACCCGTTCCCGCATAAAGCGATGCAGGAAGTGTTGCAGCAGTTTGCTCACGTCAAGGATTTTGTCTGGTGCCAGGAAGAGCCGC  >  NC_000913/760861‑761334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A