Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F9 I0 R1 260 102.5 2178490 93.2% 2030352 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,727,771 C→G 16.1% intergenic (+15/‑66) nemA → / → gloA chromate reductase, quinone reductase, FMN‑linked, N‑Ethylmaleimide reductase, old yellow enzyme/glyoxalase I, Ni‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,727,7710CG16.1% 152.2 / 3.8 76intergenic (+15/‑66)nemA/gloAchromate reductase, quinone reductase, FMN‑linked, N‑Ethylmaleimide reductase, old yellow enzyme/glyoxalase I, Ni‑dependent
Reads supporting (aligned to +/- strand):  ref base C (44/16);  new base G (5/10);  total (50/26)
Fisher's exact test for biased strand distribution p-value = 5.94e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAACCGAAGAAGCGGTGATTGAAC  >  NC_000913/1727537‑1728005
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           
tttCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCCCAGCGTGCC‑GCAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAgcggcg                                                                                                                                                                                                                                         >  1:653500/1‑239 (MQ=255)
tttCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGACGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTAAGCTTAAACACCAGCGTGCC‑GAAAGTTTCTACGGTGTCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTACTCCAACATTGCCGgcggcg                                                                                                                                                                                                                                         >  1:495624/1‑239 (MQ=255)
      cgttccgttttggggggagggggCATACAAAGTAGAAAAATGGGAAACGCTGGTGGGCAAAGGG‑TTAATTGGTGGGGTGGCATTTGGTCGTGACTGGATTGCGAACCGGGATCGGGTCGCCCGCTTGCAGCGCAAAGCTGGGCTTACCCCACAGCGTGCC‑GAAAGTTTCTAGGGTGGCGGCGCGGAAGGTTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAgc                                                                                                                                                                                                                                   <  2:609697/217‑1 (MQ=255)
         tCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGGGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CACTTACCAGACGTTGTAATCAAACATTGCGACCGGCGTAAAgccg                                                                                                                                                                                                                                 >  1:230109/1‑238 (MQ=255)
           cGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAgcgccc                                                                                                                                                                                                                               >  1:149530/1‑234 (MQ=255)
                   ggCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAACGTTTTTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGCTTACCCCACGTTTTAATCCAACATTGCGAGGGGCGGaaaacgccgcccatacta                                                                                                                                                                                                                      >  2:654757/1‑224 (MQ=255)
                                    agcaGAAAACGCTGAAACGATGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGGGACTGGATTTGGAACACGGATCTGGTCGCCCGGTTGCAGCGCAACGCTGAGCTTATCGCCCAGCGTGAC‑GAAAGTTTCAACAGTGGCGGCGCGGAAGGCGATC‑‑C‑‑CGATTACCCCACGTTCTAAACCACACTTTCGAGTGGGGTCACGGCGCCGCAAGCAAAGAACAACATTTTGAAt                                                                                                                                                                                                      >  2:713507/4‑238 (MQ=255)
                                    aGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTCATCCAACATTGCGAGCGGGGGAAACCCGCCCCTCTACTAAAAAAAAATTTTaaa                                                                                                                                                                                                       >  2:757579/1‑237 (MQ=255)
                                    aGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGaa                                                                                                                                                                                                       >  1:13508/1‑237 (MQ=255)
                                    aGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGAGCGGACGGCTATA‑‑CCACGATTACCCGACGTTGTAATACAACATTGCGACGGGCGTAAAACCGCCGCCATAATaaaaaacaaatttaaa                                                                                                                                                                                                       >  2:347407/1‑227 (MQ=255)
                                    aGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑GTAATTGATGCGGTGGCATTTGGTCGTGACTGGCTGGCGAACCCGGATCTCGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGACGCGGAATGCCATA‑‑C‑‑CGCGTACCCGAGGCTTCACTACAAAATTGCGGCGGGGGTCAAGCCCCCGGTCTACCAAATCAAAATATTTaaca                                                                                                                                                                                                     >  2:154984/1‑237 (MQ=255)
                                     gTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACCGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTCTA‑‑C‑‑CGATTACCCGCCGTTGTAATACAACATTGGGAGGGGCGTAAAGCCCCGCCTTTAGTAAAAAAAAATTTTGaa                                                                                                                                                                                                       >  2:637775/1‑236 (MQ=255)
                                     gTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAAAATTTTGAATCt                                                                                                                                                                                                    >  1:466496/1‑239 (MQ=255)
                                      tAGAAAACGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCACAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGATCCTACGGTGCCGGCCCGGAAGGCAATA‑‑C‑‑AGTTTACCCGACGTTGTATTCCACCAATTCGAGGGGCGTAACGCCCCCCCCA‑ACATAAACAACAattttacatact                                                                                                                                                                                                  >  2:157639/1‑231 (MQ=255)
                                      tAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGaa                                                                                                                                                                                                       >  1:259358/1‑235 (MQ=255)
                                      tAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGACGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGCCGTTGGTATCCAACATTGCGGGCGGCGTAAAACCCCCCCAATACAAAAAAAAAATTTTaaacagt                                                                                                                                                                                                   >  2:369271/1‑235 (MQ=255)
                                       aaaaaaaCGTGAATCGCTGATCGGCAAAGGG‑TTAATTGATGGGGTGGCATTTGGTCGGGACTGGTTTGCGAACCGGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCCCAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGt                                                                                                                                                                                                  <  1:1032039/237‑1 (MQ=255)
                                           aaaGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAg                                                                                                                                                                                               >  1:192535/1‑238 (MQ=255)
                                            aaGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTTTAATCCAAAATGTGGAGCGGCGTAAAGCCCCCCCTTTACTAAAAAAAAATTTTAAAATTGTTAGcc                                                                                                                                                                                             >  2:843146/1‑239 (MQ=255)
                                                tGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGTAAGGCTATA‑‑C‑‑CGATTACCCGCCGTGGTAATCCAACACTTCGAGCGCCGTAAAGCCGCCGCAATACTAAAACACCATTTTTAATcagataacaatat                                                                                                                                                                                         >  2:20583/1‑227 (MQ=255)
                                                tGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACCTTTTGAATCTGTTAGCCAttt                                                                                                                                                                                         >  1:463855/1‑239 (MQ=255)
                                                  gaacccgttgtgggaaaGGGTTTAATTGGTGCGGGGGCTTTTGTGCGTGAGTGGTTGCCGAACCCGGATCGGGCCCCCCGCTGGCAGGCAAAAGCTAGGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGGGGGGGCGCGGAGGGCTATA‑‑C‑‑CGATTCCCCGACGTTGTAATCCAACATTGCGAGGGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCAtttt                                                                                                                                                                                        <  2:610005/225‑1 (MQ=255)
                                                  aaaCGCTGATCGGAAAAGGG‑TTAATTGATGCGGTGGCATTTGGGCGGAGCTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCAtttt                                                                                                                                                                                        <  1:349873/238‑1 (MQ=255)
                                                   aaCGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAAAAACATTTTGAATCTGTTAGCCATTTTGa                                                                                                                                                                                      >  1:356459/1‑239 (MQ=255)
                                                          aTCGGCAAAGGG‑GTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCAGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GCAAGTTTCTACGGTGGGGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGCCGTTGTAATCCAACATTGCGGGCGGCGTAACGCCGCCGCCATACTAAAACAACATTTTTAAACTGTAAGACATTATGGGGAaaaa                                                                                                                                                                               >  2:109790/1‑239 (MQ=255)
                                                                     g‑TTAATTGGGGCGGTGGCATTTGGTCGTGCCTGGATTGCGAACCCGGATCTGGTCCCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCAAAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATC‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGt                                                                                                                                                                      <  1:654757/237‑1 (MQ=255)
                                                                     g‑TTAATTGATGCGGGGGCATTTGGTCGTGACTGGATGGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCGCAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGt                                                                                                                                                                      <  1:709118/237‑1 (MQ=255)
                                                                      ttttgttgtggCGGTGGCATTTGGTCGTGAGTGGAGTGCGCACCTGGATCTGGGCCCCCGTGTGCGGGGCAAAGCTGAGTTTAACCCACAGCTGGCA‑GAATGTTGTGACGGTGGCGGGGGGGAAGGTTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGGGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGtc                                                                                                                                                                     <  2:168603/228‑1 (MQ=255)
                                                                         atttgttgcgtgtgcttttGGTGGTGAGTGGGTTGCGACCCCGGATCTGGTCCCCCGCTTGCAGCGCAAAGCTGGGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGtcttct                                                                                                                                                                 <  2:259358/223‑1 (MQ=255)
                                                                               tGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGGGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTCGGGATAAAAAGATCCGGATTCTTCaaa                                                                                                                                                            >  2:166816/1‑236 (MQ=255)
                                                                               tGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATa                                                                                                                                                            >  1:762829/1‑238 (MQ=255)
                                                                               tGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAAAATTTTGAATCAGTTTGCCATTTTGAGGATAAAAAAATGCGGctttttacaac                                                                                                                                                           >  2:761231/1‑234 (MQ=255)
                                                                               tGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCCTTACTAAAAAAACATTTTAAATCTGTTAGCCCTTTTGGGGATaaaaaaaggggcctctttcaaac                                                                                                                                                           >  2:867569/1‑221 (MQ=255)
                                                                               tGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGGAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTAGATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTTTTCAAAc                                                                                                                                                           >  1:674119/1‑239 (MQ=255)
                                                                                gCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATAcc                                                                                                                                                          >  1:26671/1‑239 (MQ=255)
                                                                                     ggCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACACCATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATActctgcg                                                                                                                                                     >  1:729194/1‑233 (MQ=255)
                                                                                           gggtgttGACTGGATTGCGAACCCGAATCTGGTCGCCCGCTTGCCGCGCAAAGCTGAGCTTAACCCACAGGTTGCC‑GAAATTTTCTACGGTGGCGGCGGGGAAGGCTATA‑‑C‑‑CGATTACCCGACTTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGt                                                                                                                                              <  1:843146/233‑1 (MQ=255)
                                                                                                gtgacgtgttgcggccccggtttCTGGTGCCCCGTTTGCGGCGCAAGGCGGAGCTAAACCACCAGCGTCCC‑GAAGGTTTCGAGGGGGCGGGCGGGGAAGGCTATC‑‑C‑‑CGATTACCCGACTTTGTAATCCAACATGGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCg                                                                                                                                         <  2:13508/217‑1 (MQ=255)
                                                                                                 tGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACCTTTTGAATCTGTTACCCCTTTTAGGGGTAAAAAAATGCGTCTTCTTCAAACAATCA‑TGCGCGTTGGCGa                                                                                                                                        >  2:144461/1‑239 (MQ=255)
                                                                                                 tGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAAAAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGCCTTCTTCTTCCCATGC‑TCCGCGTTGGCGa                                                                                                                                        >  1:924733/1‑239 (MQ=255)
                                                                                                        aTTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGACTCTGTTAGCCATTTTGTGGATAAAAAAATGCGTCTTCTTCATACCAAGC‑TGCCGGTTTGCGATTTGCaa                                                                                                                                 >  2:30694/1‑239 (MQ=255)
                                                                                                        aTTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAAAATTTTGAATCTGTTAGCCATTTTGAGGTTAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTCGGCGATTTGCa                                                                                                                                  >  1:648123/1‑238 (MQ=255)
                                                                                                                 ccGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTTCAACGCTCCATc                                                                                                                        >  1:737176/1‑239 (MQ=255)
                                                                                                                 cagtctttggccccccGCTTGAGCCGCAAAGGTAGGCTTACCCCCCCG‑GTGCCGGAAAGTTTGTGGCGTGGGGGCGCGAAAGGTTATT‑‑C‑‑CGTTTCCCCGACTTTGTAATCCACAATTGCGGGGGGCTTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATc                                                                                                                        <  2:332645/226‑1 (MQ=255)
                                                                                                                      tttggcccccccCTTGCAGCGAAAAGCGGGGTTAACCCCCCGGCGGGCC‑AAAAGTTTCTCCGGGGGCGGGCGGGAAGGCTATA‑‑C‑‑CGATTCCCCGACGTTGTAACCAAACATTGCGAGGGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGAttt                                                                                                                   <  2:924733/231‑1 (MQ=255)
                                                                                                                          gTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GACAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTAGAGGATAAAAAGGTACGTCTTCTTCAAACCATGC‑TGCGCGTTGGCGATTTGCACAGATCCATCGGTTTTtat                                                                                                               >  2:479834/1‑239 (MQ=255)
                                                                                                                          gTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGTTAAAAAGATGCGTCTTCTTCATACCATGC‑TGCCCGTTGGCGATTTGCAACGccttctccttttaacc                                                                                                               >  1:818029/1‑224 (MQ=255)
                                                                                                                           tCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAaaca                                                                                                                                                                                                                 <  1:854751/141‑1 (MQ=255)
                                                                                                                           tCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAaaca                                                                                                                                                                                                                 >  2:854751/1‑141 (MQ=255)
                                                                                                                               ccGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCGACGCTCCATCGATTTTTATACCaa                                                                                                          >  1:820562/1‑239 (MQ=255)
                                                                                                                                  cTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTTCGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTCAAGCCGCCGCTATACTAAAACAACCTTTTGAATCTGTTAGCCATTTTGCGGATAAAAAGATGCGACTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGGTTTTTATACCAAAGt                                                                                                       >  1:285886/1‑239 (MQ=255)
                                                                                                                                     ggcgcgaAAAGGTGGGTTTACCCCACAGGGGGCC‑AAAGGTTTCGACGGGGGCGGGCGGGAAGGTTAAC‑‑C‑‑CGATTCCCCGACTTTTAAATCCAACATTGCGAGGGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCt                                                                                                    <  2:703272/236‑1 (MQ=255)
                                                                                                                                          gaaaggcggggCTTAACCCCAGGGGTGCC‑AAAAGTTCTAAGGGTGGCGGGCGGGAAGGTTATC‑‑C‑‑CGATTCCCCGACGTTGTAACCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCa                                                                                               <  2:653500/229‑1 (MQ=255)
                                                                                                                                                        aaCCCACAGCGCGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGCTAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGTATGAAATGGCTGCGt                                                                                 >  1:312690/1‑239 (MQ=255)
                                                                                                                                                             acaGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGCTTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGCGGATAAAACGATGCGTCTCCTTC‑TTCCATTCATGCGCGTTGCCGGTTTCCACCCCTCCCTCGTTTTTTTTCCCAAAGGACTGGGGATGAAActcccactaacaag                                                                            >  2:293566/1‑227 (MQ=255)
                                                                                                                                                             acaGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAGACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGCTTTGCAACCCCCCATCCATTTTTTTACCAAAGTGCTGGGCATGAAACTACTGCGTACCac                                                                            >  2:212298/1‑238 (MQ=255)
                                                                                                                                                             acaGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCa                                                                             >  1:128133/1‑238 (MQ=255)
                                                                                                                                                                   tGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTTTACCAAAGTGCTGGGCATGAAAAGGCTGGGGACCAACGaaaa                                                                      >  2:215964/1‑239 (MQ=255)
                                                                                                                                                                         aaattttttcggggggggggggggAAGGTTTTA‑‑C‑‑CGTTTCCCCGAGTTTGAAATCCAACATTGGGAGGGGCGAAAGGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCgg                                                                 <  2:463855/217‑1 (MQ=255)
                                                                                                                                                                         aaaGTTTCTCGTGTGGGGGCGCGAAGGGATATC‑‑C‑‑CGATTCCCAGACGTTGTAATCCAAAAGTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCgg                                                                 <  1:582943/239‑1 (MQ=255)
                                                                                                                                                                          atttttcagcgggggcgcgggggAAGGTTATC‑‑C‑‑CGTTTCCCCGACGTGTTAATCCAACATTGAGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCgg                                                                 <  1:43638/217‑1 (MQ=255)
                                                                                                                                                                           gtttttcgggggggggggcgggAGGGTTATA‑‑C‑‑CAATTCCCGGACGTTTAAATCCAAGATGGCGAGGGCGGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGaa                                                               <  2:149530/219‑1 (MQ=255)
                                                                                                                                                                             tttggtcgggggggggggggAAGGCTTAC‑‑C‑‑CGTTTCCCCGACGTTGAAAACCAACATGGGGAGGGGCGTAAAGCCGCCGGTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGaa                                                               <  2:128133/219‑1 (MQ=255)
                                                                                                                                                                                cTACGGTGCCGGCCCGGAAGGCTATACCC‑‑CAATTCCCCGACGTTTTAACCCAAATTGGCGGGCGGCGTAAACCCGCCGATATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATAc                                                            <  1:347407/239‑1 (MQ=255)
                                                                                                                                                                                      tGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGCTAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCCTGAAAATGCTGCGTACCAGGGAAAACCCGGAATACAAAAACTc                                                    >  2:832500/1‑239 (MQ=255)
                                                                                                                                                                                          ggcgGGTAAGGCTATC‑‑C‑‑CTTTTCCCCGACTTTGTTATCCAATCTTGGGAGGGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTg                                                <  2:356459/239‑1 (MQ=255)
                                                                                                                                                                                           gcgcGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGt                                                                                                                                                                      <  1:426194/121‑1 (MQ=255)
                                                                                                                                                                                           gcgcGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGt                                                                                                                                                                      >  2:426194/1‑121 (MQ=255)
                                                                                                                                                                                                                 aTTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGcc                             >  1:495802/1‑239 (MQ=255)
                                                                                                                                                                                                                 aTTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAAAACTCACTGGCGTTTGTTGGCTACGGcc                             >  2:604423/1‑239 (MQ=255)
                                                                                                                                                                                                                   tACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCg                           >  1:490713/1‑239 (MQ=255)
                                                                                                                                                                                                                         aCGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAAcc                     >  1:1034825/1‑239 (MQ=255)
                                                                                                                                                                                                                                 atcagtaaTTGCGGGCGGCGTAAAGCCGCCGTTATACTAAACCAACATTCGGAATCTGTTAGCGATTTTGAGGATAAAAAGATGCGTCTTCGTCATACCATGC‑TGCGCGGTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAAGACAAATACTCACTGGCGTGTGTTGGCTACGGCCCGGAAACCGAAGAAGc             <  1:215964/232‑1 (MQ=255)
                                                                                                                                                                                                                                      aCATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAACCGAAGAAGCGGGGa        >  2:510359/1‑239 (MQ=255)
                                                                                                                                                                                                                                       cATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAACCGAAGAAGCGGTGAt       >  1:310391/1‑239 (MQ=255)
                                                                                                                                                                                                                                       cATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAACCGAAAAAACGGGGaa       >  2:185367/1‑238 (MQ=255)
                                                                                                                                                                                                                                           gCGAGCGGGGTAAAGCCGCCGGTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAACCGAAGAAGCGGTGATTGaa   <  1:791857/239‑1 (MQ=255)
                                                                                                                                                                                                                                             gAGGGGCGAAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAACCGAAGAAGCGGTGATTGAAc  <  1:637775/238‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           
TTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAACCGAAGAAGCGGTGATTGAAC  >  NC_000913/1727537‑1728005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A