Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F9 I0 R1 260 102.5 2178490 93.2% 2030352 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,872,402 T→G 12.3% V121G (GTG→GGG)  yeaJ → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,872,4020TG12.3% 320.7 / 4.3 128V121G (GTG→GGG) yeaJputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (68/39);  new base G (2/18);  total (71/57)
Fisher's exact test for biased strand distribution p-value = 9.48e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

TGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGAAAAGGTATTT  >  NC_000913/1872168‑1872640
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               
tGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTTCGCGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACACGGGCTTACGCGG‑CACACGACAAACCGCATCCACTGACTGGGACACAATTGTGGaa                                                                                                                                                                                                                                             >  1:868484/1‑239 (MQ=255)
tGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCACTAACACCCATGGCTTAAACCTGCCTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGAACCAAATGTGGaa                                                                                                                                                                                                                                             >  2:872882/1‑239 (MQ=255)
tGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CCCACTCAAAACCCCATCCCCTGACTGCGACACAATTGTGGaa                                                                                                                                                                                                                                             >  2:273892/1‑239 (MQ=255)
tGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGCCCCAATTGTGGa                                                                                                                                                                                                                                              >  1:1027594/1‑238 (MQ=255)
tGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGaa                                                                                                                                                                                                                                             >  1:269718/1‑239 (MQ=255)
      aagcagccctcccctTTTTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTgca                                                                                                                                                                                                                                       <  2:148023/227‑1 (MQ=255)
       agcactcctctcctTTCTTTTTGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGGCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTgcag                                                                                                                                                                                                                                      <  2:1079016/230‑1 (MQ=255)
       aGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTgcag                                                                                                                                                                                                                                      <  1:754305/239‑1 (MQ=255)
       aGCAGACTCCTCCTGTCATTATGATAAGTATCAGAATCAGAGTATTGCCGCGCAGGTGATGCGCGCTCTCGCTGCTGAGCAGGCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTgcag                                                                                                                                                                                                                                      <  1:309732/239‑1 (MQ=255)
          aGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCCTAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCACCCCCTGACTGCGACACAAATGTGGAAGCTgcacaac                                                                                                                                                                                                                                   >  2:84159/1‑235 (MQ=255)
               cctcctTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACCCGG‑CAAACTACAAACCCCATCCACTGACTCCGACCCAATTGTGGAAGCTGAAGCACCAtt                                                                                                                                                                                                                               >  2:625998/1‑238 (MQ=255)
                ctcctTTCATTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATACCCCCCATGGCTTCAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACCCTACAAACCGCATCCCCTGCCTGCCACACACTTGGGGCAGCTGCACCACTAttc                                                                                                                                                                                                                              >  2:833884/1‑237 (MQ=255)
                        tttATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTCCAGCCCTATTACCCGCtttt                                                                                                                                                                                                                     >  1:42209/1‑239 (MQ=255)
                          tATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGCCACAATTTTGGAAGCTGAAGCCCCATTACCCGCttttcg                                                                                                                                                                                                                   >  1:204477/1‑237 (MQ=255)
                           ttGATAAGTATCAAAATCAGAGTATTGCCGCGCATGTGCTGCGGGCTCTCGCTGCGGAGCAGTCCGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACCCCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGAt                                                                                                                                                                                                                  <  2:154461/238‑1 (MQ=255)
                               tAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAgg                                                                                                                                                                                                              <  1:273892/239‑1 (MQ=255)
                                aattttcaaaatcaggGTATTGGCGCGCAGGTGATGCGCGCCTTCGCTGCTGAGGAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAgg                                                                                                                                                                                                              <  2:577763/233‑1 (MQ=255)
                                  gTATCAGAATCAGAGTATTTCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGCGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGC‑CACACTACAAACCGCTACCACTGCCCGCGACACAATTGTGAAAACGGCACCACTCTTACCCACTTTTGACCAGGCAg                                                                                                                                                                                                           >  2:170328/1‑239 (MQ=255)
                                             agagTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCaa                                                                                                                                                                                                >  1:33584/1‑238 (MQ=255)
                                                    ttccGCGCAGGGGATGCGCGCTCTGTGGGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCGGCGAGGCTTTGGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCAGAAAGCCCCGGGCTTAAGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGACCAGGCAGTGG‑AAGGCAACCGccacc                                                                                                                                                                                        <  2:782661/237‑1 (MQ=255)
                                                       cgcgCATGTTATGGGCGCTCTCGCTGCGGAGCATGCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCGGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATCCCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAg                                                                                                                                                                                      <  1:353724/238‑1 (MQ=255)
                                                           cATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTCCAAACACCATCCACTGACTGCGACCCAATTACGGACGCACCAGCACTATTACCCCCTTTTGATC‑CGCAATGGAAAGACAACCCCCACCAgacagc                                                                                                                                                                                 >  2:331800/1‑234 (MQ=255)
                                                              gtgATGCGCGCTCCCGGTGTGGAGCAGTCGGAATGGCCGCAAGAACAGGGGCGCGCCATTTGCGAGGCTTTGGAGTCGGCCAATAACACCCATGGCTTAACCCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTa                                                                                                                                                                              <  1:1013819/239‑1 (MQ=255)
                                                              gtgATGCGCGCTCCCGCTGCTGAGCAGTGGGAAGTGTCGCAAGAACAGCGGCGCGCCATCTGCGAGCCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTa                                                                                                                                                                              <  1:31992/239‑1 (MQ=255)
                                                              gtgATGCGCCCTTCCGCTGCTGGGCAGTGGGAAGTTCCGCAAAAACGGCGGGGCGCCACCGGGAGGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCAAAAATCCCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTa                                                                                                                                                                              <  2:320281/239‑1 (MQ=255)
                                                              gggATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGGAAGGCTTTTGAGTCTTCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATCCCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTa                                                                                                                                                                              <  1:319132/237‑1 (MQ=255)
                                                                     gcccccccgcgGCTGGGAAGTGGGAAGTGTCCCCAAAACGGCGGCGCCCCAGCTGCGAGGTTTTTGGGCCCGCCAATAACCCCCATGGCTTAACCCTGACTGCCCATAAATCCCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTCCCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTc                                                                                                                                                                        <  2:543802/231‑1 (MQ=255)
                                                                        ctctCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAgg                                                                                                                                                                     >  1:997332/1‑238 (MQ=255)
                                                                             cgtgctgGGCAGTCGGAAGGGTCGCCAGAACAGCGGCGCCCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAACCCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTgg                                                                                                                                                               <  1:284899/237‑1 (MQ=255)
                                                                                     gCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGCCTGCCCATAAATCCCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGcc                                                                                                                                                        <  1:184456/238‑1 (MQ=255)
                                                                                         tCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGCCACAATTGTGGAAGCTGAAGCACTCTTACCCGCTTTTGCTCAGGCAGGGG‑AAGGAAACCGCCCACAAGATGATTCAGGTTTAGGTCTTTGGAAGGACGaaaa                                                                                                                                                   >  2:369860/1‑237 (MQ=255)
                                                                                         tCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCCCCAGGATGCTTAAGGTTCCGGGCTTGGGATGTCCGAaga                                                                                                                                                   >  1:966920/1‑239 (MQ=255)
                                                                                         tCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGGCTTGGGATGGCCGAaga                                                                                                                                                   >  1:558251/1‑239 (MQ=255)
                                                                                         tCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGCCTTGGGATGGCCGAaga                                                                                                                                                   >  1:346207/1‑239 (MQ=255)
                                                                                           ggAAGTTGCGCAAGAACGGCGGCCGGCCATCTGCGAGGCTTTTGAGTCTCCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATCCCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAagag                                                                                                                                                  <  1:793770/238‑1 (MQ=255)
                                                                                                 gtCCCCAAAACAGCGGCGCCCCATCGGCGAGGCTTTGGAGTCTGCCAATAACCCCCATGGCTTAAACCGGACGGCCCAAAAATCCCCGGGCTTCCGCGG‑CACACACCAAACCGCATCCACTGACTGCGACACAATTGGGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAAttt                                                                                                                                            <  2:1066289/238‑1 (MQ=255)
                                                                                                   cGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGCAGAGAAATTTCAc                                                                                                                                         >  1:23746/1‑239 (MQ=255)
                                                                                                       aGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGGTTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACtatt                                                                                                                                     >  2:941922/1‑239 (MQ=255)
                                                                                                              cggcgCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACCATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAATGG‑CAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGGAGAGACATTTCACTATTATCTCGa                                                                                                                              >  1:1077836/1‑239 (MQ=255)
                                                                                                                            gggggttttgggctctcCCAAAACCCCCCAGGGCGTAACCCGGCCTGCCCAAAAAACCCCGGGCTTACGCGG‑CACACTCAAAACCGCAGCCACTGACTGCGACACAATGGGGGAAGGTGCAGCACTATTCCCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCGTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCt                                                                                                                <  2:966920/226‑1 (MQ=255)
                                                                                                                                gCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTagg                                                                                                             >  1:719636/1‑236 (MQ=255)
                                                                                                                                    ttGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCCCTATTATCTCGATCTCAAAGACCCCTATGTCTAt                                                                                                        >  2:130514/1‑239 (MQ=255)
                                                                                                                                           tGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGCCTTGGGATGGCCGACGGGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTAATTTTAta                                                                                                 >  1:475399/1‑238 (MQ=255)
                                                                                                                                           tGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGCCTTGGGATGGCCGAAGAGAAATTTAACTATTATCTCGATCTCAATGACCGCTATGGCAAATTTTATg                                                                                                 >  2:380057/1‑239 (MQ=255)
                                                                                                                                            gCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGTTGATTACGTTTCAGGGCTTGGGATGGCCGAAGAGAAATTCCAATATTATCTCGACCTCAAGGAACGCAATGTTAATTTTAATGa                                                                                                >  2:100252/1‑239 (MQ=255)
                                                                                                                                            gCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATATCGATCTCAATGACCGCTATGTCTAtttttttta                                                                                                >  1:997361/1‑235 (MQ=255)
                                                                                                                                              cAATACCACCAAGGGCTTAAACCTGACTTCCCAAAAATCCCCGGGCTTACGCGG‑CCCACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAg                                                                                               <  1:872882/238‑1 (MQ=255)
                                                                                                                                                  accccccATGGCTTAACCCGTACCGCCAATAAATCCCCGGGCTTACGCGG‑ACCACAAAAACCCCCATCCACTGACTGCGACACAATTGGGGAAGCTGCAGCACTATTCCCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGt                                                                                          <  2:200541/235‑1 (MQ=255)
                                                                                                                                                  aaCCCCCATGGCTTAACCGTGCCTCCTCTTAAAACCCCGGGTTTCCGCGC‑CACACTACAACCTGCTCCCACTACGTGCGCCAAAATGGTGGAACCTGGAGAATAATTCCCCGTTTTTGAAGGGGCAGTGG‑AAGGCAACCGCCACCATGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGt                                                                                          <  2:868484/239‑1 (MQ=255)
                                                                                                                                                    ccccctgggtttAACCCGGCCTGCCAATAAAACCCCGGGCTTCCGCGG‑CACCCTACAACCCCCATCCACTGACGGCGACACAATTGGGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTa                                                                                        <  2:305422/229‑1 (MQ=255)
                                                                                                                                                    cccccGTGGTTTAAACCTGCCTGCCCATAAACACCC‑GGCTTTAGCGGACACAGTACAAACCGCGTCCACTGACGGCGACACAATTGTGGGAGCTGCAGAACTAGTCCCCGCTGTTGACCGGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTa                                                                                        <  2:204477/237‑1 (MQ=255)
                                                                                                                                                    cccccATGGCTTAACCCGTACCCCCCATAAACACCCGGGCTTACGCGG‑CACACTAAAAACCGCATCCACTGACTGCGAACCAATTGTGGAAGCTGCAGCACTATGACCCGCTTGTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTa                                                                                        <  1:833884/237‑1 (MQ=255)
                                                                                                                                                    cccccATGGCTTAACCCGGACTCCCCATAAATCCCCGGGTTTACGCGG‑CCCACAACAAACCGCATCCCCTGACGGCGACACAATTGGGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGGGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTa                                                                                        <  2:42209/237‑1 (MQ=255)
                                                                                                                                                    cccccATGGCTTAAACCTGACTGCCCATAAATCCCCGGGCTTACGCGC‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTCCCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTa                                                                                        <  1:884364/237‑1 (MQ=255)
                                                                                                                                                    cacCCATGGCTTAAACCTGACTGCCAATAAATCCCCGGGCTGACGCGG‑CACCATACAAACCGCCTCCCCTGACTGCGACACAATTGTGGAAGCTGCAGCCCTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTa                                                                                        <  1:545119/239‑1 (MQ=255)
                                                                                                                                                       ccATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGCAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTCG‑AAGGCAACCGCCACCAGGATGATTCCGGTTCAGGTCTTGGGATGGCCGAAGAGGAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTCTGAGCCGGTTAATg                                                                                     >  1:782405/1‑239 (MQ=255)
                                                                                                                                                       ccATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATg                                                                                     >  1:200541/1‑239 (MQ=255)
                                                                                                                                                         aTGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGGCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATTGGCCGGTTAATGtg                                                                                   >  2:758016/1‑238 (MQ=255)
                                                                                                                                                           ggCTTAACCCTGACGGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGGGGAAGCTGAAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTATGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGa                                                                                 <  1:942602/239‑1 (MQ=255)
                                                                                                                                                                aacccTGACTCCCCTTAATTCCCCGGGTTTACGCGG‑CACACTACAAACCGCATCCACTGACCGCGACAGAATTGTGGAAGCTGCAGCACTATTACCGGTTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACt                                                                            <  2:346207/236‑1 (MQ=255)
                                                                                                                                                                 aaCCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAAttt                                                                                                                                            <  2:306236/174‑1 (MQ=255)
                                                                                                                                                                 aaCCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACtt                                                                           >  1:8796/1‑239 (MQ=255)
                                                                                                                                                                     aaggacagcttAAATCCCCGGGCTTCCCGGC‑CACACTCAAAACCCCACCCCTTGACTGCGACACAATGTGGGAAGCTGCAGCACTATTCCCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCg                                                                       <  2:1074967/229‑1 (MQ=255)
                                                                                                                                                                       aCTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGTCTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCCCTATTACCTCGATCTCAATTACCGCTACGTCTATATTTAGGAGCCGGGTAATGTTGAAAACTTTTCggg                                                                     >  2:645205/1‑237 (MQ=255)
                                                                                                                                                                       aCTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTAcc                                                                                                                                                                                                                            >  1:657102/1‑89 (MQ=255)
                                                                                                                                                                       aCTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTAcc                                                                                                                                                                                                                            <  2:657102/89‑1 (MQ=255)
                                                                                                                                                                       aCTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGAt                                                                     >  1:802331/1‑239 (MQ=255)
                                                                                                                                                                       aCTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGAt                                                                     >  1:565474/1‑239 (MQ=255)
                                                                                                                                                                             cATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTAAGGTCTTGGGATGGCCGAAGAGAAATTTCCCTATTATCTCGATCTCAATGACCGCCATGTCTATTTTTATGAGCCGGTTAATTTTGAATACTTTggtcggaaaaa                                                               >  2:869378/1‑229 (MQ=255)
                                                                                                                                                                                aaaTGCCCGGGCTTACGGGG‑CACACTACAAACCGCTCCCACTGACTGCGACACAATTGGGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGGGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTg                                                            <  1:830738/239‑1 (MQ=255)
                                                                                                                                                                                  accccggggCTTACCGGG‑CACACTACAACCCGCCCCCCCTGCCTGCGACACATTGGTGGAAGCGGCAGCATTATGACCCGTTTTTGACAGGGCAGTGG‑AGGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGt                                                          <  2:808312/233‑1 (MQ=255)
                                                                                                                                                                                     cccGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATTACCGCTATGTCTATTTTTATTAGCCGGTTAATGTTTAATACTTTTCGATGAAAAAAGGGtcct                                                       >  2:421887/1‑239 (MQ=255)
                                                                                                                                                                                     cccGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATTAGCCGGTTAATGTTGAAAACTTTGCGATGAAAAAAGGGtcct                                                       >  2:524124/1‑239 (MQ=255)
                                                                                                                                                                                     cccGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGtcct                                                       >  1:702011/1‑239 (MQ=255)
                                                                                                                                                                                     cccGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAAAAACGGGtcct                                                       >  2:531785/1‑239 (MQ=255)
                                                                                                                                                                                        gggCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACt                                                             >  1:1074967/1‑230 (MQ=255)
                                                                                                                                                                                          gCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTCTTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGTGCCGGTTAAATTTGAATAATTTGGGATAAATAAATGGtccttcct                                                   >  2:43755/1‑238 (MQ=255)
                                                                                                                                                                                          gCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAAAACTTTGCGTTGATTAACTGTTCCTTCCTg                                                  >  1:138460/1‑239 (MQ=255)
                                                                                                                                                                                                gcgG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGTTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCa                                            <  1:380057/239‑1 (MQ=255)
                                                                                                                                                                                                     cacaCTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATAACCGCTATGTCTATTTTTTTGAGCCGGTTAATGTTGAATACTTTGCGTTGAATAACTGGTCCTTTCTACAGTCAAGaa                                        >  2:947494/1‑239 (MQ=255)
                                                                                                                                                                                                      ccactcaaaaCCCCATCCCCTTCCTCGGAAACAATTTTGGAAGCTCCAGCATTTTTCCCCGCTTTTGATCAGGCAGGGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGa                                         <  2:439142/230‑1 (MQ=255)
                                                                                                                                                                                                               aCCCCATCCACTGACTGATGAACAATTGTGGAAGCGGGAGCCCTATTACCCGGTTTTAATCAGGCAGTGG‑AAGGCAACCGCCCCCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCat                              <  1:947494/239‑1 (MQ=255)
                                                                                                                                                                                                                ccGCACCCACTGACTGACACAAAATGGGGGAAGGCGCAGCACTTTTCCCCTTTTTTGATCAGGCAGGGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCatc                             <  2:33584/239‑1 (MQ=255)
                                                                                                                                                                                                                    aTCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGt                                                                                                                                                                    >  1:503363/1‑100 (MQ=255)
                                                                                                                                                                                                                    aTCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCatcgat                          >  1:445354/1‑238 (MQ=255)
                                                                                                                                                                                                                    aTCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGAATTGGCatcgatc                         >  1:618235/1‑239 (MQ=255)
                                                                                                                                                                                                                    aTCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACT‑GTCCTTCCTGCAGTCAGGAAGTATTGGCatcggtcg                        >  1:410098/1‑239 (MQ=255)
                                                                                                                                                                                                                       cACTGACTGCCACCCAATTGGGGAAGCTGCAGCACTATTACCCGCTTTTGATAAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCa                      <  1:524124/239‑1 (MQ=255)
                                                                                                                                                                                                                           ggatgcaaaacaATTGTGGAAGCTGCAGCACTATTACCCGTTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGa                  <  1:625998/230‑1 (MQ=255)
                                                                                                                                                                                                                              tgCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTCTTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGatat               >  1:789711/1‑239 (MQ=255)
                                                                                                                                                                                                                              tgCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTTGGATGAATAAATGGTCCTTCCTGCAGTCAGGAAGAATTGGCATCGATGGAAAAGatat               >  2:759685/1‑239 (MQ=255)
                                                                                                                                                                                                                                cGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGCCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTCTTTTTATGAGCCGGTTAATGTTGAATCCTTTGCGATGACTAACTGGTCCTTCTTGCACTCAGGAAGAATTGGCATCGATTGCAAAGAAATTg             >  2:722970/1‑239 (MQ=255)
                                                                                                                                                                                                                                 gACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATg                                                                                     >  1:815110/1‑166 (MQ=255)
                                                                                                                                                                                                                                 gACACAATGGTGGAAGTTCCACCACTTTTCCCCGTTTTTTATAAGGCGGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACT‑GTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGaa           <  2:410098/239‑1 (MQ=255)
                                                                                                                                                                                                                                  cccaatttGTGTAAGCTGCCGCCATTTTCCCCGTTTTGGAACGGGCGGGGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGaa           <  2:23746/233‑1 (MQ=255)
                                                                                                                                                                                                                                   cacaATTGGGGAAGTGGCAGCTCTATTCCCCGCTTTTGATAAGCAAGTGA‑AAGGCAACCGCCACCAGGATGATTACGTTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGaa           <  1:119188/238‑1 (MQ=255)
                                                                                                                                                                                                                                   cacaATTGGGGAAGTGGCAGCACTATTACCCGTTTTTGATCAGGCAGGTG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGaa           <  1:43755/238‑1 (MQ=255)
                                                                                                                                                                                                                                   cacaATTGGGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGaa           <  1:130514/238‑1 (MQ=255)
                                                                                                                                                                                                                                   accaATTGTGGAAGCTGCACCACTATTACCCGCTTTTGAACAGGCGGGGG‑AAGGCAACCGCCCCCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTAGTTTTATGAGCCGGTTAATGTTGAATCCTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGaa           <  1:331800/236‑1 (MQ=255)
                                                                                                                                                                                                                                           tgGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGAAAAGGTAttt  >  1:994464/1‑239 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               
TGCAGAAAGCAGACTCCTCCTTTCTTTATGATAAGTATCAGAATCAGAGTATTGCCGCGCATGTGATGCGCGCTCTCGCTGCTGAGCAGTCGGAAGTGTCGCCAGAACAGCGGCGCGCCATCTGCGAGGCTTTTGAGTCTGCCAATAACACCCATGGCTTAAACCTGACTGCCCATAAATACCCGGGCTTACGCGG‑CACACTACAAACCGCATCCACTGACTGCGACACAATTGTGGAAGCTGCAGCACTATTACCCGCTTTTGATCAGGCAGTGG‑AAGGCAACCGCCACCAGGATGATTACGGTTCAGGTCTTGGGATGGCCGAAGAGAAATTTCACTATTATCTCGATCTCAATGACCGCTATGTCTATTTTTATGAGCCGGTTAATGTTGAATACTTTGCGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGAAAAGGTATTT  >  NC_000913/1872168‑1872640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A