Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F9 I0 R1	260	102.5	2178490	93.2%	2030352	226.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,241,926	T→A	11.8%	T110S (ACC→TCC)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,926	0	T	A	11.8%	372.7 / 6.9	133	T110S (ACC→TCC)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base T (68/42); new base A (4/13); total (74/59)
Fisher's exact test for biased strand distribution p-value = 3.80e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

CGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCG‑CAAA‑CTGGTTAAAAGAGACCAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAAT  >  NC_000913/4241689‑4242163

cgaaaCGGGAAAAAAAAGTGTTTACGCAGTAAATTAAAGTTTGCCCAAAAATAACCG‑CAAC‑CTTGTTAAAAGAGACCAGTTCCCCGCG‑AATAT‑TAGCTGGAGCCATTTCCCCATTATACATTGGCGAAACCATTGAGTCAAAACCAACCCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGT‑TTTAT‑AGAGGT‑a                                                                                                                                                                                                                                                   <  1:363455/236‑1 (MQ=255)

cGGAAGGGGAAATAAAATAGTTTAGGCAGTAAACTAAAAGTTGCCAGAAAATAAGCG‑AAAA‑CTGGTAAAAAGAGACCATTTCCCCGGG‑AATAG‑GAGCTGGAGCCAGTTCCGCAATATACATGGGCGAGAGCATTGAGGCTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑a                                                                                                                                                                                                                                                   <  1:787933/239‑1 (MQ=255)

   aaCGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCG‑CAAA‑CTGGTTAAAAGAGACCAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AAAGGT‑aaaa                                                                                                                                                                                                                                                >  1:472529/1‑239 (MQ=255)

    acggaaaaaaaaaTAGTTTACGCAGTAAACTAAAAGTTGCCAGAAAATAATGG‑AAAA‑CTGTTAAAAAGAGACCAGTTCCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACAAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAAc                                                                                                                                                                                                                                               <  1:937376/230‑1 (MQ=255)

          aaTAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCG‑CAAA‑CTGGTTAAAAGAGACCAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCCGGACATAACCTGCCCGATAAACAGG‑CAAACGGTTGCCC‑GGG‑GTTAT‑AAAAGA‑AAAACCAAGt                                                                                                                                                                                                                                          >  2:910416/1‑238 (MQ=255)

                    tttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCG‑CAAA‑CTGGTTAAAAGAGACCAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑Gc                                                                                                                                                                                                                             >  1:32132/1‑239 (MQ=255)

                     ttgcGCAGAAAACAAAAAGTTGCCAAAAAATAAGCG‑CAAC‑CTGTTAAAAAGGGACAAGTTCCGCAAG‑AATAT‑GAGCTGGAGCAAGTGCAGAAATAACCATGGGCGAGCGCATTGAGACTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑Gc                                                                                                                                                                                                                             <  1:985952/235‑1 (MQ=255)

                     ttccgaaGTAACCTAAAGGTTGCATGAAAATAATCG‑AAAA‑CTGGTTAAAAGAGACCATTTTCCGGCG‑AATAT‑GAGCTGGAGCAAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATCCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑Gc                                                                                                                                                                                                                             <  1:562842/232‑1 (MQ=255)

                      tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCG‑CAAA‑CTGGTTAAAAGAGACCAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAg                                                                                                                                                                                                                           >  1:655517/1‑239 (MQ=255)

                      tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCG‑CAAA‑CTGGTTAAAAGAGACCAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGCG‑AAAACCAAGTTC‑TGGCCAG‑GCAg                                                                                                                                                                                                                           >  1:744278/1‑239 (MQ=255)

                      tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCG‑CAAA‑CTGGTTAAAAGAGACCAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAAAAGG‑CACAGTTTTGTCC‑GGG‑TTTAA‑AAAGGT‑AAAACCAAGTTC‑TGGCCAgaaaa                                                                                                                                                                                                                            >  2:401106/1‑235 (MQ=255)

                       accaagaaaaCAAAAAG‑TCCCCAAAAAAAAACGCCAAA‑CTGGTAAAAAGAGCCCAGTTCCCCGCG‑AAATT‑GAGCTGGAGCCAGTCCCCAAAATTACATTGGCGAAGGAATTGAGCCTAAACCACCGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGa                                                                                                                                                                                                                          <  1:542076/232‑1 (MQ=255)

                        cGCAGTAAACTAAAAGTTGCCCGAAAATAATCG‑CAAA‑CTGGTTAAAAGAGACCAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CCCAGTGTTTTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGa                                                                                                                                                                                                                          >  1:456357/1‑238 (MQ=255)

                             tAAACTAAAAGTTGCCCGAAAATAATCG‑CAAA‑CTGGTTAAAAGAGACCAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGCTAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGA‑AAAACCAAGTTC‑TGGCCAG‑GCAGaaaccac                                                                                                                                                                                                                    >  2:590428/1‑234 (MQ=255)

                             tAAACTAAAAGTTGCCCGAAAATAATCG‑CAAA‑CTGGTTAAAAGAGACCAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGAAAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AAAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑ccaaa                                                                                                                                                                                                                   >  2:588656/1‑236 (MQ=255)

                                     aaGTTGCCCGAAAATAATCG‑CAAA‑CTGGTTAAAAGAGACCAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACCTAACCTGCCAGATAAACAGG‑CACAGCGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CATaaac                                                                                                                                                                                                                 >  1:370225/1‑233 (MQ=255)

                                       ttgtccaaaaaaTAAT‑G‑CAAACCTGGTAAAAAGAG‑CCAGTTTCCCGGA‑AAAATGGGGCGGGACCCAGGTCCGAAAATAACATTGGCGGGAGAATGGAGGTTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGTGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATa                                                                                                                                                                                                         <  2:460141/231‑1 (MQ=255)

                                          gCCCGAAAATAATCG‑CAAA‑CTGGTTAAAAGAGACCAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGGTAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTT‑TGCCCAG‑GCAGAA‑CCTACACCAGGAGTaaaa                                                                                                                                                                                                      >  1:39295/1‑239 (MQ=255)

                                           cccGAAAATAATCG‑CAAA‑CTGGTTAAAAGAGACCAGTTTCCCGCG‑AACAT‑GAGCTGGAGCCAGGTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTACCGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACAAGAaaaaaaaa                                                                                                                                                                                                     >  2:1055375/1‑239 (MQ=255)

                                                  atctcgcaaaaa‑CTGTTTAAAAAAACCCATTTCCCCGGG‑AATAG‑GAGGGGGAGCCAGTCCCGAAATATACATTGGCGAAAGCATTGAGGCTAAACCACCGCCACAACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGa                                                                                                                                                                                               <  2:456357/230‑1 (MQ=255)

                                                       cagaaaa‑GTGGTTAAAAGAAACCATGTGCCGGGA‑AATCG‑GGGCGGGCGCCAGTCCCGCATTATGCATTGGGGAGGTCATTGGGACTAAACAACCGCAAAACCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGGCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGAcagca                                                                                                                                                                                          <  2:279736/235‑1 (MQ=255)

                                                        g‑CAAT‑CTGTTAAAAGGAGACAAGTCTCCGGGC‑AATAT‑GAGCTGGGCCTAGGTCACAAATATACATTGGTGAGTGCATTGAGGCAAAACCAACGCCAATGCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGGCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAT‑CCTACACCAGAAATAAAAAACAGGAcagcag                                                                                                                                                                                         <  1:442064/238‑1 (MQ=255)

                                                         gaaaa‑CTGGTTAAAAAAGACCGTTTCCCCGCG‑AATAT‑GAGTGGGAGCAAGTTCCGAAATAAACATTGGGGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACagcagc                                                                                                                                                                                        <  1:757430/237‑1 (MQ=255)

                                                           caaaatttttAAAAAGGACCCTTTTCCCGGGAAATTG‑GGGCTGGAGCCAGTCCCGAAATAACCATTGGGGAGAGCATGGAGGTAAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACagcagc                                                                                                                                                                                        <  2:180345/231‑1 (MQ=255)

                                                            aaacgtgTTAAAAGAGACCAGTTTCCCGGG‑AATGT‑GATCTGGACCCAGTTCCGAAATATACATTGGCGAAAGCATTAAGGTCAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAgcagca                                                                                                                                                                                       <  1:531682/232‑1 (MQ=255)

                                                                  gTTAAAAGAGCCCAGTTTCCCGAG‑AATAG‑GAGGTGGAGCCAGTTCCGAAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTaa                                                                                                                                                                                <  1:175224/239‑1 (MQ=255)

                                                                  gTTAAAAGAGACCAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAAAAGGACAGCAGCAATCTTaa                                                                                                                                                                                >  1:1077611/1‑239 (MQ=255)

                                                                   gaaaaaGAGACAAGGTCCCCGGA‑AATAT‑GAGCTGGACCCAGTGCCGAAATATACATGGGCGAGAGCATTGAGTCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTaa                                                                                                                                                                                <  1:272603/236‑1 (MQ=255)

                                                                      aaggagaCCAGTTCCCCGGA‑AATAT‑GAGCTGGCGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGa                                                                                                                                                                            <  1:190062/236‑1 (MQ=255)

                                                                            aacAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCACTTCCGCAATATTCATTGGCGAGAGCACTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGCTCAATAACAAATTTCGGGACATAACCTACCAGAGTCATGGG‑CACACTGTTGTCC‑GGG‑ATTCT‑CGAGGG‑AAAACCCAGTTC‑AATCCAG‑GCAGAA‑CGTACACGAGCGAGCGAACACAGGAACGCAGCAATATTAATGGAATCacg                                                                                                                                                                      >  2:694977/3‑239 (MQ=255)

                                                                            aCCAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCacg                                                                                                                                                                      >  1:598804/1‑239 (MQ=255)

                                                                                   tCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACAAGAAATAAAAAACAGGAAAGCAGCAATCTTAAGGGAATCCCGACGACCg                                                                                                                                                               >  1:1008141/1‑239 (MQ=255)

                                                                                       gcg‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGc                                                                                                                                                           >  1:879854/1‑239 (MQ=255)

                                                                                                      ggAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACCGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATa                                                                                                                                              >  1:614012/1‑239 (MQ=255)

                                                                                                                             tGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGGTACTGCAATAACAACC‑GAGGGCACCGCCgatga                                                                                                                      >  1:987761/1‑239 (MQ=255)

                                                                                                                              ggCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAACTAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTAATGCAATAACCACC‑GAGGGCACCCCCgatgaa                                                                                                                     >  2:658585/1‑238 (MQ=255)

                                                                                                                              ggCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCcatgat                                                                                                                     >  1:575875/1‑239 (MQ=255)

                                                                                                                                cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGCTGATGc                                                                                                                   >  1:511968/1‑239 (MQ=255)

                                                                                                                                cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGc                                                                                                                   >  1:655673/1‑239 (MQ=255)

                                                                                                                                cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAACGGTTACTGCAATAACCACC‑GAGGGACACGCCgagaaggc                                                                                                                   >  1:584092/1‑233 (MQ=255)

                                                                                                                                cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCAAAGCGGTTCCTGCAATAACCACC‑GAGGGCACCGCCGATGATGc                                                                                                                   >  1:1028656/1‑239 (MQ=255)

                                                                                                                                cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACAACCGAAGCGGTAACTCCAATAACCACC‑GAGGGAAacgcccaggagga                                                                                                                   >  2:1082042/1‑231 (MQ=255)

                                                                                                                                  agagatttGGGGCTAACCCAACCAAAATCCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTTTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑GGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATACCCACC‑GAGGGCACCGCCGATGATGCa                                                                                                                  <  1:910416/232‑1 (MQ=255)

                                                                                                                                  agagCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGGGAATCACGACAACCGAAGCGGTTACTGCAAAAACCACC‑AACGGCACCCCCgacacagaaa                                                                                                                 >  2:358730/1‑231 (MQ=255)

                                                                                                                                    agCGTTGAGGAAAAACAAGCACAAAGACCGCAAATAATGCAATAAATAAAAAATTCGGGGAAATAACCTGCCAGATAAACAGG‑CCCAGTGTTGTCA‑GGG‑TTTAT‑AGGGGT‑AAAACCAATTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACATCGCAAATCTTAAGTGAATCACGACGACCGAAGCGTTTACTGCAATAACCACC‑GGGGGCACCGCCGATGATGCAAc                                                                                                                <  1:886565/238‑1 (MQ=255)

                                                                                                                                    agCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAAcc                                                                                                               >  1:334190/1‑239 (MQ=255)

                                                                                                                                    agCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATAACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAAcc                                                                                                               >  1:212103/1‑239 (MQ=255)

                                                                                                                                         tGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGAc                                                                                                                                                                                              <  1:319552/156‑1 (MQ=255)

                                                                                                                                         tGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGAc                                                                                                                                                                                              >  2:319552/1‑156 (MQ=255)

                                                                                                                                         gtgaaaaaaacccccacaaaacccGACAATAAGGAAAAAAAAAAAAAATTCCGGAACATACCCTGCCAGATAAACAGG‑CACAGTGTTGT‑C‑GGG‑GTTAA‑AAAGGTAAAAACCAAGTTC‑GGCCCAG‑CAAGAC‑CCAACACCAAAAAAAAAAAACAGGACAGCACAAATCTAAAGTGAACCACGACGACCGAAGCGGTTACGGCAATACCCCCC‑GAGGGCACCGCCGATGATGCAACCAATCa                                                                                                          <  2:212103/217‑1 (MQ=255)

                                                                                                                                                aaaCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGATTCACGACGACCGAAGCGGGTACTGCAAGAACCACC‑AAGGGCACCGCCGATGCGCCAACCAATCAGAgcgcg                                                                                                   >  1:496784/1‑238 (MQ=255)

                                                                                                                                                 aaCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTAACTGCAATAACCACC‑GAGGGACCCGCCGATGATGCAACCAATCAGAGCGCt                                                                                                   >  1:1079087/1‑238 (MQ=255)

                                                                                                                                                       cGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTGACTGCAAAAACCCCC‑GAGGGCACCCCCGATGAGGAAACCAAACAGAGCACTGGCCACg                                                                                            >  2:347918/1‑239 (MQ=255)

                                                                                                                                                          aaaaccccgaaaatggtgaaaaaaaaaCAAATTCGGGGAAATACCCGCACAAATAAACGGG‑AACAGTTGTGCCC‑GGG‑TTAAA‑AGAGGA‑AAAACCAAGTTC‑TGGCAAG‑GCAGAA‑CCAACACCAAAAAAAAAAAACAGGACAGCAACAATGTTAAGTGAATCCCGACGCCCGAAGGGTTTACTGAAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaa                                                                                         <  2:655517/218‑1 (MQ=255)

                                                                                                                                                                  ccAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGGTACTGTCAAAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCGGGCCACGCAAAACCCTaa                                                                                 >  1:927660/1‑239 (MQ=255)

                                                                                                                                                                         tGCGATAAATAACAAATTCCTGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTa                                                                                                                                                      <  2:889544/164‑1 (MQ=255)

                                                                                                                                                                         tGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTa                                                                                                                                                      >  1:889544/1‑164 (MQ=255)

                                                                                                                                                                          gcgcTAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGCAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCCCC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACCGGGAGt                                                                         >  1:161259/1‑239 (MQ=255)

                                                                                                                                                                          gCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGt                                                                         >  1:56962/1‑239 (MQ=255)

                                                                                                                                                                             ataaataaCAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCAACGCACAACCCTAACAGGGAGTTg                                                                       >  1:78479/1‑238 (MQ=255)

                                                                                                                                                                              aaaaaaaCAAATCCCGGGACAAAACCTGCCAGAAAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAA‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGCAAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGc                                                                     <  1:688057/238‑1 (MQ=255)

                                                                                                                                                                                          tCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGATGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCAAGACGACCGAAACGGTTACTGCAATAACCACC‑GAGAGCACCGCCGATGATGCAACAAATCAGCGCGCTGGCCACGCAAAACCCAAACAGGGAGTGGGCAACGGATGCACt                                                         >  2:763493/1‑239 (MQ=255)

                                                                                                                                                                                            cgtgACATAACCTGCCAGATAAACAGA‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACAAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCCGGCACAACCCTAACAGGGAGTTGGCAGCGGATTCACTTa                                                       >  1:880251/4‑239 (MQ=255)

                                                                                                                                                                                            cGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTa                                                       >  2:698661/1‑239 (MQ=255)

                                                                                                                                                                                             gggACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGTTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCCCTTaa                                                      >  1:1023294/1‑239 (MQ=255)

                                                                                                                                                                                                   tAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAg                                                                                                                                                                                                              >  2:749449/1‑82 (MQ=255)

                                                                                                                                                                                                   tAACCTGACAGATAAACAGG‑CACAGTGTTGTCC‑GGT‑TTAAT‑AGAGGT‑AAACCCAAGTTC‑TGGCAAG‑GCAGAC‑CCTACACCAAAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTtg                                                <  1:590428/239‑1 (MQ=255)

                                                                                                                                                                                                    aaCCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCACCCCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGtttttg                                               >  2:654970/1‑237 (MQ=255)

                                                                                                                                                                                                       ccgcccagaaaaCAGGCCACAGTTTTGTCG‑GGG‑TTTAA‑AAAGGA‑AAAACCAAGTTC‑GGGCCAG‑GCAAAC‑CCAACCCCAGAAAAAAAAAACGGGACAGCAGCAATCTAAAGGGAACCCCGACGACCGAAGCGGTTACGGCAATACCCCCC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTgg                                             <  2:525606/230‑1 (MQ=255)

                                                                                                                                                                                                         gCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCGGCGGATTCCCTTAAGTTTTTTGGAGc                                          >  1:914806/1‑239 (MQ=255)

                                                                                                                                                                                                         gCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGAGCTGGCCACGCAAAACCCCAACAGGGGGTTGGCCGAGGATTCCCTTAAGTTTTTTGGAga                                          >  2:85667/1‑238 (MQ=255)

                                                                                                                                                                                                                aaaacgggctagttgtgttCC‑GGG‑TTTAA‑AGGGGA‑AAAACCAAGTT‑‑TGGGCAGAGCAGAC‑CCTAACACAAAAAAAAAAAACGGGAAAGAAACACTCTAAAGTGAAACACGACGACCGAAGCGGTTACTGCAATACCCCCA‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGca‑ac                                      <  2:268162/218‑1 (MQ=255)

                                                                                                                                                                                                                aaaCAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGCGCA‑ACAAAg                                  >  1:1002895/1‑239 (MQ=255)

                                                                                                                                                                                                                 accggg‑CACAGTTTTGCCC‑GGT‑TTTAT‑AGGGGA‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACAAGAAATAAAAAACAGGACAGCAGCCACCTTAAGGAAACCCCGACGACCGAAGCGGTTACTGCAATACCCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGa                                 <  2:71041/235‑1 (MQ=255)

                                                                                                                                                                                                                 aaacgg‑CACAGTGGTGGCC‑GGG‑TTTAA‑AGGGGT‑AAAACCAAGTTC‑GGGCCAG‑GCAAAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGGGAATCACGACGACGGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGa                                 <  1:752826/235‑1 (MQ=255)

                                                                                                                                                                                                                 aaaaggaccgggggttgcCG‑GGG‑TTTAA‑AGAAGA‑AAAACCAAGGTT‑GGGCCAG‑GAAACC‑CAAACCCAAAAAAAAAAAAACGGGAAACCAAAAATGTAAAGGGAATCACAGAGACCAAAGCGGTTCCGACAAAAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAg                                  <  2:928574/226‑1 (MQ=255)

                                                                                                                                                                                                                 aaCAGG‑GACCGGTTTTTCC‑GGGTTTTAA‑AGAGAT‑AAAACCAAGTTC‑GGGCAGG‑GCAAAA‑CCCACCCAAGAAAAAAAAAACAGGCCAGAAGAAACCTAAAGGAAATCCCGAAGACGGAAGGGGTTACT‑CAAAAACCCCCGGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAg                                  <  2:1028656/239‑1 (MQ=255)

                                                                                                                                                                                                                  cgggg‑CACGGTGTTGTCC‑GGG‑TTTAA‑AGAGGT‑AAAACCAAGTTC‑GGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGa                                 <  1:46764/235‑1 (MQ=255)

                                                                                                                                                                                                                    aGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGAcagca                                                                                                                                                                                          >  2:67303/1‑85 (MQ=255)

                                                                                                                                                                                                                    aGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGAcagca                                                                                                                                                                                          <  1:67303/85‑1 (MQ=255)

                                                                                                                                                                                                                            gtgtTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGATACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGACGGTATTGAg                       >  1:95857/1‑239 (MQ=255)

                                                                                                                                                                                                                              ttttttc‑GGG‑TTTTTAAGAGGA‑AAACCCAGTTTC‑GGGCCAG‑CCACAA‑CCTAAACCAAAAAAAAAAAACGGGACAACACAAATCTAAAGGGAACCACGCCGACCGAAGCGGTTCCTCAAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGACGGTATTGAGt                      <  2:598804/233‑1 (MQ=255)

                                                                                                                                                                                                                              ttttgcagggg‑TTTAA‑AGAGG‑‑AAAACCCAGTTCTTGGGCAG‑CGAAAA‑CCTACCCCAGAAATAAAAAACAGGAAAACAGAAATCCTAAGTGAATCACGACGACAGACGCGTTTACTGAAATACCCACC‑GGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGACGGTATTGAg                       <  2:710634/230‑1 (MQ=255)

                                                                                                                                                                                                                               gtttacaggg‑TTGAT‑AGAGGT‑AAACCCAATGTC‑AGACCGG‑GGAGAA‑CCAACACAAAAAATAAAAAACGGGACAGAAGCAATCTTAAGTTAATCACGACTGCCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGACGGTATTGAg                       <  1:79937/230‑1 (MQ=255)

                                                                                                                                                                                                                                tttccgggg‑TTTAA‑AGAGG‑‑AAAACACAGTTT‑GGGCCAGAACAAAA‑ACAACCCCAAAAAAAAAAAACAGGACGGCAGCAATCTTAAGTGACACCCCCCGACCGAAGGGGTTCCTGCAAAAACCCCC‑GAGGCCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGACGGTATTGAGTGa                    <  2:78479/233‑1 (MQ=255)

                                                                                                                                                                                                                                ttccc‑GGGTTTTAA‑AGAGGA‑AAAACCAAGTCC‑GGGCCAG‑GCAAAA‑CACACACCAGAAAAAAAAAGAAGAAAAGCAGAAATCTTAAGTGAATCCGGACGCACCATGCGGTTACGCCAATAACCACC‑AGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGACGGTATTGAGTGa                    <  2:33074/236‑1 (MQ=255)

                                                                                                                                                                                                                                tgtCCGGGG‑TTAAA‑AGATGA‑AAAACAAAGTTC‑GGCCCAG‑GCAGAC‑ACTAACCAAAAAAAAAAAAAAAGGACAGCAGAAACGTAAAGAAAATCACGACCACAGAAGCGGTTACTGCAATACCCACA‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGACGGTATTGAGTGa                    <  2:161259/239‑1 (MQ=255)

                                                                                                                                                                                                                                 gtCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGGGTTGGCCGCGGATTCACTTAAGTTTTGTGGAGCA‑AAAAAAACGGCATTGAg                       >  2:46764/1‑234 (MQ=255)

                                                                                                                                                                                                                                    c‑GGT‑TTTAT‑AGAGG‑‑AAAACCTAATTT‑TGGCCCC‑GGACAACCCTACACAAAAAAAAAAAAACGGGACACCAACACTTGTAATGAAATCACAACGCGCGATGCGTTTACTGCAAAACCCCCA‑GGGGTCACCGCCGATGATGCAACCAGTCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGACGGTATTGAGTGCCTCaa               <  2:1068982/239‑1 (MQ=255)

                                                                                                                                                                                                                                    c‑GGG‑TTTAA‑AGAAGA‑AACACCAAGTTC‑GGGACAG‑GCAAAC‑CCAACACCAAAAAAAAAAAACAGAACAGCACAAATTTAAAGTGAACCCCGACGCCCGAAGGGTTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGACGGTATTGAGTGACTCaa               <  2:879854/239‑1 (MQ=255)

                                                                                                                                                                                                                                        g‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGACGGtctgga                        >  1:928574/1‑222 (MQ=255)

                                                                                                                                                                                                                                        g‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGACGGTATTGAGTGACTCAACAg            >  1:757951/1‑239 (MQ=255)

                                                                                                                                                                                                                                         aaaaaagaagggT‑AAAACCAATTTC‑GGCCCAG‑GAAAAA‑CCTACCCCAGAAAAAAAAAACAGGACAGCAGCAATCTAAAGTGAACCCCGACGACCGAAGCGGTTACTGAAATACCCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGACGGTATTGAGTGACTCAACa             <  1:300130/229‑1 (MQ=255)

                                                                                                                                                                                                                                           ttAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCAACGACAAACCCTACAAGGGAGTTGGCAGCGGGTTCACTTAAGTTTTGTGGAGCACACAAA‑ACGGAATTggaggacccaaaaga           >  2:32407/1‑224 (MQ=255)

                                                                                                                                                                                                                                                    gT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCt                                                                                                                                                                                   <  1:534541/64‑1 (MQ=255)

                                                                                                                                                                                                                                                    gT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCt                                                                                                                                                                                   >  2:534541/1‑64 (MQ=255)

                                                                                                                                                                                                                                                    gT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAACACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGACGGTATTGAg                       >  1:1077944/1‑218 (MQ=255)

                                                                                                                                                                                                                                                    gT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCCCTTAAGTTTTGGGGAGCA‑ACAAAGACGGAATTGAGGGACCCAAAAGGACCGGaaaa  >  2:671499/1‑238 (MQ=255)

                                                                                                                                                                                                                                                    gT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGAGGGAATTGAGGGAATCAAAAGAACCGGaaaa  >  2:806720/1‑238 (MQ=255)

                                                                                                                                                                                                                                                    gT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAACGGATTCACTTAAGTTTTGTGGACCA‑ACAAAGACGGATTTTAGGGAACCAAAAGTACCGGaaaa  >  2:520615/1‑238 (MQ=255)

CGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCG‑CAAA‑CTGGTTAAAAGAGACCAGTTTCCCGCG‑AATAT‑GAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGG‑CACAGTGTTGTCC‑GGG‑TTTAT‑AGAGGT‑AAAACCAAGTTC‑TGGCCAG‑GCAGAA‑CCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACC‑GAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCA‑ACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAAT  >  NC_000913/4241689‑4242163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A