Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F17 I0 R1 124 155.2 3079696 98.3% 3027341 226.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,256,084 T→C 11.7% M128V (ATG→GTG)  plsB ← glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,256,0840TC11.7% 293.4 / 4.1 124M128V (ATG→GTG) plsBglycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (32/69);  new base C (2/13);  total (42/82)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

ACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTT  >  NC_000913/4255849‑4256317
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           
aCGACAGCCGGACGTGGACGGGAAATGTG‑CATCCGCGCCCGCCGCGCCAGTTTCTGAGCGATAGTTTTAGCTGTGCCGTGTTACCCCGCCATGCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAATCGGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTGCGCCGTTAAGCATACGCAGCGGCGGGTTCACTGCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AATCATC‑Ac                                                                                                                                                                                                                                         <  2:1026425/239‑1 (MQ=255)
 cGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCCGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTTCCTTCCCCCCTTT‑CCTGCCCCGGCCCCC‑CCCC‑AAAcacc‑ac                                                                                                                                                                                                                                         >  2:1514048/1‑238 (MQ=255)
   gCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTT‑TACCCCCCGGCGCGC‑GCCC‑AAAAATC‑ACCg                                                                                                                                                                                                                                       >  1:326162/1‑238 (MQ=255)
    agcccAGACGTTGCCGGGCAAAGTG‑CATACGCGCCCCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGa                                                                                                                                                                                                                                      <  2:663636/235‑1 (MQ=255)
     acccaagcGTTGGCGGGAAAAGGT‑AATACGCGCCCCGCGCCCAGGTTTCTGAGCAATAGTTTTCCCCTTCCCGTGTTCGCCCGCAAAAGGGCGCAGCGAAACGGACGGCGAAAAACGCAAAAAACTGTCGCGCCCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGaca                                                                                                                                                                                                                                    <  2:191499/232‑1 (MQ=255)
      gCCAGCCGTGGACGGGCAAAGTG‑CATCCGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTACCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGaca                                                                                                                                                                                                                                    <  2:211670/238‑1 (MQ=255)
      gCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑TACCCCCCCGCGCGC‑GACC‑CAACATC‑Accccca                                                                                                                                                                                                                                    >  1:340067/1‑234 (MQ=255)
       ccAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGACTTTTT‑TACCCCCCGGCTCGC‑GACC‑CAAAAAT‑ACCGacac                                                                                                                                                                                                                                   >  1:121036/1‑238 (MQ=255)
        cAGCCGTTG‑CGGGGCAAGTGCCATCCGCGCCCCGCGCGCCAGTTTCTGACCGATAGTTTTAGCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGaca                                                                                                                                                                                                                                    <  2:326162/236‑1 (MQ=255)
        cAGACGTTGACGGGCAAAGTG‑CATCCGCGCCCCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGaca                                                                                                                                                                                                                                    <  1:329513/236‑1 (MQ=255)
           ccGTTGACGGGCAAAGGC‑CATACGCCCCACGCGCGCCAGTTCCGGGGCAATAGTTTTACCCGTGCGGTGTTCTCCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGc                                                                                                                                                                                                                               <  2:882806/237‑1 (MQ=255)
           aCGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTTGCCTTTTT‑TCCCCCCCGGCGCGC‑GACC‑ACAAATA‑Acccgccacggc                                                                                                                                                                                                                               >  1:413979/1‑229 (MQ=255)
             tgtGACGGGAAAAGTG‑CATACGCGCCCCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCTTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCAcc                                                                                                                                                                                                                            <  1:525736/237‑1 (MQ=255)
                 aCGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACCTCGCCTTTTT‑TACCCCCCGGCGCGCGGCCC‑AAACA‑C‑ACCGACACCGGCAccc                                                                                                                                                                                                                           >  2:149660/1‑235 (MQ=255)
                   gggCAACGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGc                                                                                                                                                                                                                      <  1:1376778/239‑1 (MQ=255)
                   ggcgaaaGTG‑CACACCCGCCACGCGCCCCAGTTCCGGAGGGTTGGTTTTATCCGTGCGATGTCCCGCCGCCATACGGCGCTGCGAACCTGACGGCGAGAAACACACAAAACTGTCGCGGCCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTCCGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTg                                                                                                                                                                                                                       <  2:538490/234‑1 (MQ=255)
                         aGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAACGGCGGGTTTACATCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAAAATC‑ACCGCCACTGGCACCATCTGCACAT‑c                                                                                                                                                                                                                >  1:886626/1‑238 (MQ=255)
                               aTACGCGCCGCGCGCGCCAGTTGCGGAGCGATAGTTTTATCCTTGCCTTGTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGAtt                                                                                                                                                                                                          <  1:623283/239‑1 (MQ=255)
                               aTACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACGGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGAt                                                                                                                                                                                                           <  1:705008/238‑1 (MQ=255)
                                tACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCCC‑CACC‑AAACATC‑CCCGGCACTGGGACCCTCTGCCCCA‑CCCGAttt                                                                                                                                                                                                         >  1:1260624/1‑239 (MQ=255)
                                  cgcgcccgccggccaggTTTCTGGCGGATGGTTTTGCCCTTGCCGTGTTCACCCGCCGTCCGGCGCAGCGAACCTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTg                                                                                                                                                                                                        <  2:1321591/222‑1 (MQ=255)
                                    cgcCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCCTTTT‑CACCCCCCGGCCGGC‑GACC‑AAACAAC‑ACCCACACCAGCTACCCCTGCACAC‑CCCGATTTgggg                                                                                                                                                                                                     >  2:341639/1‑238 (MQ=255)
                                      ccACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGTCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCCCTTCGCCTTTTT‑CAACCCCCCGCGCGC‑CTCC‑AAACAAC‑ACACACACCGGCACCactcctccataccagctttggttt                                                                                                                                                                                                    >  2:592152/1‑216 (MQ=255)
                                      ccACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTTACTTTCCCTTTTT‑CACGCCCCGGCGAGC‑GAAC‑AAAAATC‑ACCCACACTGGCACCACATTCACAT‑CCCGATTTgggat                                                                                                                                                                                                    >  1:703419/1‑236 (MQ=255)
                                      ccACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑TACGCCCCCGCCCGC‑GACC‑AAACATC‑ACCCGCACTGGCACCCCCTGCCCAT‑CCACATTTTGGtt                                                                                                                                                                                                    >  1:978033/1‑238 (MQ=255)
                                      ccACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCCCCTTTTT‑TACGCCCCGGCGCGC‑GACC‑CAACAAC‑ACCGCCACTGGGCCCATCTGCACCT‑CCCAGTTTTGGTTg                                                                                                                                                                                                   >  1:817642/1‑239 (MQ=255)
                                          gcgcgcCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTAc                                                                                                                                                                                               <  1:466687/239‑1 (MQ=255)
                                             cgcCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGCTCACTTCGCCTTTTT‑TACGCCCCCGCGCGC‑GCCC‑CAACATC‑ACCGACACCCGCACCATCTGCCCAT‑CCAGATTTTGGTTGCTACgcg                                                                                                                                                                                            >  1:191928/1‑237 (MQ=255)
                                              gcCAGTTTTTGAGCGATGTGTTTATCCGTGCCGTGTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTg                                                                                                                                                                                           <  2:487540/239‑1 (MQ=255)
                                              ccaggttgcgagccGATATTTTTATCCGTGCCGTGTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTg                                                                                                                                                                                           <  2:1354283/226‑1 (MQ=255)
                                               ccAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑CACC‑AAAAACC‑ACCCACACTGGCCCCATCTGCACCT‑CCAGATTTGGGTTTCTACGGTGc                                                                                                                                                                                          >  1:688769/1‑239 (MQ=255)
                                                    ttCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAGCTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCaa                                                                                                                                                                                        <  1:1058989/236‑1 (MQ=255)
                                                          ggaaTAGTTTTATCCGTGCCGGGTGCACCCGCAAAACGGCGAAGCGAAACGGACGGCGAGAAACGCACAAAACTTTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGAt                                                                                                                                                                                <  2:924803/235‑1 (MQ=255)
                                                          gCGATAGTTTTATCCGTGCCGTGTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGAt                                                                                                                                                                                <  1:1514048/238‑1 (MQ=255)
                                                          gCGATAGTTTTATCCGTACCGTGTTCATCCGCCGTACGGCGCGGGGAAACTGAGGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGAt                                                                                                                                                                                <  1:471439/238‑1 (MQ=255)
                                                          cggATAGTGTTAGCCGGGCCGTTTTCATCCACCATAGGGCGCAGGGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGAt                                                                                                                                                                                <  2:826538/236‑1 (MQ=255)
                                                             aTAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑TACTCCCCCGCCCGC‑GCAC‑AAACAACAACCAACACT‑GCACCCTCTGCCCATCCCAATTTTGGGTTCTTCGGGTGCAAAGC‑AGATAGTc                                                                                                                                                                            >  2:663356/1‑239 (MQ=255)
                                                             aTAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACCTCGCCTTTTT‑TACGCCCCGGCGCGC‑GACC‑CAACATC‑ACCGACACTGGCACCATCTTCACCT‑CCCGATTTGGGTTGGTACGGTGCAAATCGAGATAGTc                                                                                                                                                                            >  1:422738/1‑239 (MQ=255)
                                                                  tgaacccgtgccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTgg                                                                                                                                                                        <  2:69454/233‑1 (MQ=255)
                                                                   ttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                                                      <  1:1067615/239‑1 (MQ=255)
                                                                   ttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                                                      <  2:228098/239‑1 (MQ=255)
                                                                   ttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                                                      <  1:1189943/239‑1 (MQ=255)
                                                                   ttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                                                      <  1:498482/239‑1 (MQ=255)
                                                                   ttATCCGGGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                                                      <  1:1334398/239‑1 (MQ=255)
                                                                   ttACCCGTGCCGTGTTCATCCGCCACCCGGCCCCGCGAACCTGACGGCGAGAAACGCACAAACCTGTCGCGCCCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                                                      <  2:157295/239‑1 (MQ=255)
                                                                   ttACCCGTGCCGGGTCCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCAAAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                                                      <  2:340067/239‑1 (MQ=255)
                                                                   ttACCCGGGCCGGGGCCACCCGCCATACGGCGCAGCGAAAATGACGGCGAAAAACGCACAAAACTGCCGCGACCGGG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                                                      <  2:413979/239‑1 (MQ=255)
                                                                   tgaTCCGGGACGTGTGCACCCACCATACGGCACAGCGAAACTGACGGCGAGAAACGGAAAAAGCGGTCTCGACCGAG‑CCACA‑GTCCAGCGAAAAA‑TTTC‑TGTACGCCAGTAAGCAGACGCAGCGGCGGGTTCAGTTGGCCTTTTT‑CCCGCCCCGGCGCGC‑GGCC‑AACCATC‑CCCGACGCTGGCACCAGCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                                                      <  2:1088057/237‑1 (MQ=255)
                                                                   taaccggtgccgggTTAACCCGCCATACGGCGCAGCGAAACGTACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCGCA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                                                      <  2:789170/233‑1 (MQ=255)
                                                                    gagcagcgacgtgGTCGTCCGCCATGCGGCGCGACGAAACTGGCGGCGAGAAACGCGCAACACTGGGGGGACCGGG‑CCGAA‑GTACAGCGAAAAA‑TTTC‑TGGACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCATTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCGGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                                                      <  2:34262/229‑1 (MQ=255)
                                                                      tCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCgaga                                                                                                                                                                                 <  1:559598/225‑1 (MQ=255)
                                                                          tgccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTT‑TTCCCCCCCGCGCGC‑CACC‑AAACATC‑ACCCACACTCGCACCATCTCCACAC‑CCAGATTTTGGTTGCTTCGGTGCCAATCAAGATAGACGTGGGACAGGttt                                                                                                                                                               >  2:572923/1‑238 (MQ=255)
                                                                           gccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCCGCGCCC‑GCCC‑CAACATC‑ACCGCCACCGGCACCATCTCCCCCC‑CCCCATTTTGGGTGCCACGGCGGCAACCGAGATAGTCGTGGGACAGCCTaa                                                                                                                                                              >  2:129079/1‑239 (MQ=255)
                                                                           gccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCCCTTCGCCTTTTT‑TACCCCCCGGCGCGC‑GGCC‑CAACACC‑CCCCACCCCGGCACCCTCTCCCCCT‑CCCGATTTTGGGTGCTACGGGGGAAATCGAGAAAGGCGTGGGACAGCATaa                                                                                                                                                              >  2:868270/1‑239 (MQ=255)
                                                                                gtTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑TACGCCCCCGCGCGC‑CACC‑CAAAATA‑ACCGACCCTGGCACCCCCCCCCCCA‑CCCGATTTGGGTTGCTTCCGTGCAAAAAGCGAAAGTCGTgggaattgctcttctac                                                                                                                                                         >  2:559598/1‑224 (MQ=255)
                                                                                gtTCATCCGCCATACGGCGCAGCAAAACTGACGGCGAGAAACGCACAAAACGGCCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGGGGCGGGTTCACTTCGCCTTTTG‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGAc                                                                                                                                                         <  1:1093365/239‑1 (MQ=255)
                                                                                gtTCACCCGCCATACGGCGCAGCGAAACGGACGGCGAAAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGAc                                                                                                                                                         <  1:700338/239‑1 (MQ=255)
                                                                                 tgctaccGCCAAGCGGGGCAGCGAAACTGTGGGGGAGAAACCAACAAAACGGTGGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATGCGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑GCCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGAct                                                                                                                                                        <  2:1406699/234‑1 (MQ=255)
                                                                                  tCATCCGCCATGCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGAct                                                                                                                                                        <  1:1159545/238‑1 (MQ=255)
                                                                                    aTCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCCCTTCCCCTTTTT‑TACGCCCCCGCGCGC‑GACC‑CAACATC‑ACCCACACCGGCACCCTCTCCACAT‑CCCCATTTGGGGAGGTAACGTGCGAATAGCGATAGTCGGTGAACAACTTAAATAACtctt                                                                                                                                                     >  2:1410055/1‑239 (MQ=255)
                                                                                         ccATACTGCGCAGCGAAACTGACAGCGAGAAACGCACAAAACAGTCGCTACCGAG‑CCACA‑GTACAGCGAAAAC‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGATTCCTTTGCCGCTTT‑TTCGGCCCCGCTCGC‑GGCC‑CTACATCAACCGACCCTTCCA‑CACCTCTACAC‑CCCGACTTGGATTGCTTCGGTGCCAATCGAGATAACCGCTGACACGTTTAATAGACCCTTCttt                                                                                                                                                 >  2:5728/1‑238 (MQ=255)
                                                                                           aTACGGCGCAGCGAAGCTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                                                              <  1:412119/239‑1 (MQ=255)
                                                                                           aTACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                                                              <  1:244740/239‑1 (MQ=255)
                                                                                           aTACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                                                              <  1:1479587/239‑1 (MQ=255)
                                                                                           aTACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCCTTTT‑TTCCCCCCGGCGCGC‑GGCC‑AAACATC‑ACCCACCCTGGCACCCACCTCCCAC‑CCAGATTTTGGTTGCTTCCGGGCAAATCGAGATAGTGGTGGGACAGCTTAATAAACACTTCTTTTgg                                                                                                                                              >  2:1479587/1‑239 (MQ=255)
                                                                                           aTACGGCGCAGCGAAACTGACGGCGAGAAACGCAAAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                                                              <  1:1195483/239‑1 (MQ=255)
                                                                                             aCGGCGCAGCGAAACTGACGGCGAGAAACGCAAAAAACGGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCg                                                                                                                                            <  1:482940/239‑1 (MQ=255)
                                                                                             aCGGCGCAGCGAAACGGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCg                                                                                                                                            <  1:868270/239‑1 (MQ=255)
                                                                                                 cgcAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCgtgt                                                                                                                                         <  1:1358143/238‑1 (MQ=255)
                                                                                                 cgcAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACCTCGCCTTTTT‑CACCCCCCCGCCCGC‑GACC‑AAAAAAC‑AACCGCACCGGCCCCCCCCTCACAA‑CCACACTTGGGGTGCTTCGGTGCAAACCCAGATAATCGTGGGACCGCTTAATAGCATCTTTTTTCTGCGTGTa                                                                                                                                        >  2:1382704/1‑239 (MQ=255)
                                                                                                  gcACCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTCCGGCGTGTaa                                                                                                                                       <  1:878974/239‑1 (MQ=255)
                                                                                                    aGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑TACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCCGATTTTGGTTGCTACGGGGCAAATCGAGATAGTCGTGGAACAGCTTAATAAACTCTTCTTTTGGCGTGTAATa                                                                                                                                     >  1:1432040/1‑239 (MQ=255)
                                                                                                       gAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAgg                                                                                                                                   <  1:1286181/238‑1 (MQ=255)
                                                                                                                         gcaaaaaacCTGTCGCGCCCG‑G‑CCCCAGGTACA‑CCAAAAA‑TTTCTTGTCCGCCGTAAGGCATCCGCGGGGGGGGTTTCACTCCGCTTTTTT‑CCCCCCCCGGCGCGC‑GACA‑AAACCCC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGAAACAGCTTAATAGACTCTTCTTGCGGCGTGTAATAGGTGAACACACGCGGCccgcc                                                                                                                <  2:1120687/234‑1 (MQ=255)
                                                                                                                         cccaaaaaaCCGTCGCGCCCGGGCCCGCA‑GTCCAGCAAAAAT‑TTTG‑TGTCCCCGGTAAAGCACCCGCCGCGGCGGTTCCACTTCCCCTTTTC‑CCCCCCCGGGCGCGC‑ACCA‑AAACACC‑CCCGACACGGGCACCATCTGAACAT‑CCAGATTGGGGTTGCTACGGTGAAAATCGAGATAGGCGGGAAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATGGGTGAACACACGCGGCccgc                                                                                                                 <  2:817642/234‑1 (MQ=255)
                                                                                                                            aaaaacCTGCCCCGACCGAG‑CCACA‑GAACAGAAAAAAT‑TTTT‑TTGCCCCCGTAAAGCATCCGCGGCGGGGGTTTCCCTCCGCTTTTTC‑CCCCCCCCGGCGCCC‑GACA‑AAACATC‑CCCGACACGGGCACCATCTGCACAC‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGTTTAATAGACTCTTCTTTCGGCGTGAAATAGGTGAACACCCGCGGCCCGCCGtg                                                                                                             <  2:556543/237‑1 (MQ=255)
                                                                                                                                aaCTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAt                                                                                                                                                                                                                  <  1:639408/135‑1 (MQ=255)
                                                                                                                                  ccgccgccaccggccccACA‑GTACAGCAAAAAT‑TTTC‑TGTCCACCGTAAACATTCCGCGGGGGCGGTTTCCCTCCCCCTTTTC‑CCCCCCCCGCGGCGC‑GACA‑AAACACC‑CCCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTCCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGtgaatga                                                                                                        <  2:565428/224‑1 (MQ=255)
                                                                                                                                         gACCGGG‑CCACA‑GTACAGCGAAAAA‑TTTT‑TGTACGCCTTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAg                                                                                                <  1:282692/239‑1 (MQ=255)
                                                                                                                                         gACCGAG‑CCAAA‑GTACAGCAAAAAT‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCTTTTTT‑CCCGCCCCGGCGCGC‑GACC‑AAACACC‑CCCGACACTGGCACCATCTGCACAT‑CAAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAg                                                                                                <  1:1248105/239‑1 (MQ=255)
                                                                                                                                          gccGAG‑CCACA‑GTACAGAAAAAAT‑TTTT‑TGTCCCCGGTTAAGCATCGGCGGGGGGGGTTTCCCTTCCCTTTTTC‑CCCGCCCCGGCGCGC‑GAAA‑AAACATC‑CCCGACCCGGGCACCATCTGCACAT‑CAAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACCCACGCGGCCCGCCGTGAATGAACACATAg                                                                                                <  2:1331567/237‑1 (MQ=255)
                                                                                                                                            cGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTAACTTTCCCTTTTT‑CACGCCCCCGCGCGC‑GACC‑AAAAATC‑AACGCGCCCGGCACCATCTGCACCT‑CCAGATTTAGGTTGCTACTGTGCAAAAACAGATAGTGGGTGGACAACTTAATCAACTCTTTGTTAGGCGAGTAATAGGAGAAGCAACACGGGCTGCCGTGAAGAAACAAATAacgg                                                                                             >  2:520836/1‑238 (MQ=255)
                                                                                                                                             gAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCCCTTCGCCTTTTT‑CACCCCCCCGCGCGC‑GCCC‑AAACATC‑ACCCACACCGGCACCCTCCTCACAT‑CCAGAATTGGGGTGCTTCGGGGCAAATCGAGATAGACGTGGGACAGCTTAATAGACTCTTCTTTCTGCGGGTAATAGGGGAAAAAACACGGGCCCCCCTGAATTAacaacaaacgcc                                                                                            >  2:501511/1‑230 (MQ=255)
                                                                                                                                              cgagcccacgTACAGAGAAAAATTTTT‑TGGCCGCCGTAAAGAATCGGCGGGGGGGGGTTCCTTCCGCTTTTTT‑CACCCCCCGCGGCG‑‑GACCAAAACACC‑ACCGACCAGGGCACAATCTGCACAT‑CCAGATTGGGGTTGCTACGTGGCAAATGGAGATAGTGGTGAAACAGTTTAATAGACTCTTCTTTCGGCGTGAAATAGGGGAACACACGCGGCCCGCCGTGAATGAACACATAgcg                                                                                              <  2:1148883/230‑1 (MQ=255)
                                                                                                                                                           aGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCCTCTGCACAT‑CCAGATTTTGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGGGTAATAGGTGAACACACCCGGCCCGCCGCGAATGAACACATAGCGCGGCAGTAGCggg                                                                                 >  1:537349/1‑236 (MQ=255)
                                                                                                                                                           aGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTT‑TACGCCCCCGCGCGC‑GCCC‑CAACATC‑ACCGCCACCGGCACCCTCTGCACAT‑CCCGATTTGGGTTGCTACCGTGCAAATCGCGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGAGAACACACGCGGCCCCCCGTGGATGATACCATAGCGCTGCAGTAGCGTg                                                                                 >  2:731713/1‑238 (MQ=255)
                                                                                                                                                           aGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGGTGCTAATGTGCAAAACGATATATTCTTCTATTCGCGTTATAGACTCTTCTTTCGGCGGGTAATAGGGGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGGAGCGTg                                                                                 >  1:900844/1‑238 (MQ=255)
                                                                                                                                                             cGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTTCCCTTTTT‑TACGCCCCCGCGCGC‑GGCC‑CAACATC‑ACCCACACCGGCACCCTCTTCACCT‑CCCGATTTTGGGTGCTACGGTGCAAAACGAGGAAGTCGTGGGACAGCTTAATAAACTCTTCTTTTGGCGGGTAATAAGGGAACACACCCGGCCCCCCGGGGATGCACACCTAACGCGGCAGAAGGGCGcc                                                                               >  2:24404/1‑238 (MQ=255)
                                                                                                                                                             cGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑TACGCCCCCGCGCGC‑GACC‑AAACATC‑ACCCACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAAACGAGATAGTCGTGGAAAAGCTTAATAGACTCTTCTTTCTGCGGGTAATAGGGGAAAACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCg                                                                              >  1:954243/1‑239 (MQ=255)
                                                                                                                                                                          tGTCCGCCGTTAAGCATACGCAGCGGCGGGTTCATTTCCCCTTTTT‑CCCGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACGGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAg                                                                   <  1:501511/239‑1 (MQ=255)
                                                                                                                                                                             cccccGTAAACCATACGCGGCGCGGGGTTCCTTTCCCTTTTTC‑CCCGCCCGGGCGGGC‑GACA‑AACCACC‑ACCGACACGGGCCCCATCTGCACAT‑CCAGATTTGGGTTGCTCCGGTGCAAATCGAGAAAGTCGTGAAACAGCTTAATAGCCTCTTCTTTCGGCGTGAAATAGGTGACCCCACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCgg                                                                <  2:900844/236‑1 (MQ=255)
                                                                                                                                                                                        cATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCttt                                                                                                                                                 <  1:1042940/147‑1 (MQ=255)
                                                                                                                                                                                         atccGCAGCGGCGGGTTCCCTTCCCCTTTTC‑CACCCCCCGGCGCGC‑AACA‑AAACATC‑CCCGACACTGGCCCCATCTGCACAT‑CCAAATTGGGGTTGCTACGGTGAAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGt                                                     <  1:35781/235‑1 (MQ=255)
                                                                                                                                                                                         aacccccgcggcgGGTTCCCCTGCCCTTTTTCCCCCCCCCGGCGCGC‑GCCA‑AAACACC‑CCCGACACTGGCCCCATCTGCACAT‑CAAGATTGGGGTGGCTCGGGTGAAAATCGAGATAGTCGTGAAACAGCTAAATAGACTCTTCTTTCGGGGTTTAATAGGTGACCACCCGCGGCCCGCCGTGAATGAACATATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGt                                                     <  2:1217878/232‑1 (MQ=255)
                                                                                                                                                                                                   gtaaaagaaccagctcTTTTC‑CCCCCCCCGGCGCGC‑GACA‑AAACACC‑ACCGACACTGGCACCATCGGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGGGGAACAGCTTAATAGCCTCTTCTTTCGGCGTGTAATGGGTGAACCCACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTc                                           <  2:834698/223‑1 (MQ=255)
                                                                                                                                                                                                    ggtgctccgtctcccctttgacgcGCCCCCGCGCGC‑CGCC‑AACCCTC‑CCCGACACGCGCCCGTTCTGCACAT‑CGAGATTTGGGTTCCTGCGGGGCAACGCGAGATAGTCGGGGAAAAGCTTAATAGACTCTTCTTCCGGCGGTAAATAGGTCAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTc                                           <  2:640906/215‑1 (MQ=255)
                                                                                                                                                                                                                            gCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGa                                 >  1:834698/1‑224 (MQ=255)
                                                                                                                                                                                                                                 ggCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCa              >  1:273146/1‑238 (MQ=255)
                                                                                                                                                                                                                                      gc‑gACA‑AAACACC‑CCCGACACTGCCACCATCTGCACAT‑CCAGATTTGGGTTGCTAGGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAAt        <  1:616669/239‑1 (MQ=255)
                                                                                                                                                                                                                                        gcaacaaaaCATC‑CCCGACACGGGCCCCATCGGCACCT‑CCAGATTTGGGTTGCTCCGGTGCAAATCGAGATAGTCGGGGAACAGCTTAATAGACTCTTCTTGCGGCGTGAAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAAt        <  1:663356/233‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           
ACGGCAGCCAGACGTTGACGGGCAAAGTG‑CATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGC‑GACC‑AAACATC‑ACCGACACTGGCACCATCTGCACAT‑CCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTT  >  NC_000913/4255849‑4256317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A