Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F110 I1 R1 11 49.5 1781824 94.3% 1680260 142.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 701,362 G→T S78Y (TCC→TAC)  nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913701,3620GT100.0% 132.4 / NA 46S78Y (TCC→TAC) nagCN‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/21);  total (25/21)

TGCTGGGTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGG  >  NC_000913/701227‑701501
                                                                                                                                        |                                                                                                                                           
tGCTGGGTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCCCTTTGCTGCTGAGATCAAACAGAGTGCTGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGCAAT‑GGCGGGCTTCGCTGACGCTGTAGATAgc                                                                                                                                      >  1:594393/1‑143 (MQ=255)
    gggTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGCTGTAGATAGCgagg                                                                                                                                  >  1:212398/1‑143 (MQ=255)
           ttCGGGCAGCGGTTAATGTTTTTCGGCGAGTACTTTGCTGCTGAGATCAACTAGAGTGATGGTGGCGTCATTACGACCAAGCCGTACGCCGATGGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGcccc                                                                                                                           <  1:566889/143‑1 (MQ=255)
                   gCGGGTAATGTTCGTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAg                                                                                                                   <  2:809277/143‑1 (MQ=255)
                    cGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAgg                                                                                                                  <  2:847831/143‑1 (MQ=255)
                     gggTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGc                                                                                                                 <  2:193613/143‑1 (MQ=255)
                       gTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGCTCAAACAGCGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGGGGAAAT‑TGCGGGTTGCGGTGACGACGTAGATAGCGCAGCGGCCCCCGGCGGAGGccc                                                                                                               >  1:736598/1‑142 (MQ=255)
                       gTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCct                                                                                                               >  2:136029/1‑143 (MQ=255)
                        tAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATAAAACAGAGTGATGGTGGCGTCATGACGACGAGGCCGTACGCCGCCTG‑TAGAAAGTATCCCAGCTTTGGTGGCGATGTAGCTGTCGCGGCGGCCCCCGGTGGAGGCctg                                                                                                              >  2:647058/1‑143 (MQ=255)
                          aTGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCctgct                                                                                                            >  2:828580/1‑143 (MQ=255)
                           tGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGTCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGGTGTAGATGGCGCGGCGGCCCCCGGTGGAGGCctgctg                                                                                                           >  2:542688/1‑143 (MQ=255)
                                 tctGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCTATGGCTTGGACAA‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAAc                                                                                                     <  2:242780/143‑1 (MQ=255)
                                    gcgaGCACTTTGCTGCGGAGGTCAAACAGTGTGGTGGTGGCGGCAGGGCGACCAAGCCGTACGCCGATTGCGTGGAAAT‑GGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAActtc                                                                                                  <  1:823323/140‑1 (MQ=255)
                                     ccAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGGCCCCGGTGGAGGCCTGCTGATCAActtct                                                                                                 <  2:846389/143‑1 (MQ=255)
                                      cAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAActtctt                                                                                                >  2:303053/1‑143 (MQ=255)
                                       aGCTCTTTGCTGCTGGGATCGAGCAGGTTCATGTTGGCGTCTTGACGACCTAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCttt                                                                                               <  1:564419/143‑1 (MQ=255)
                                        gCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTg                                                                                              >  2:614292/1‑143 (MQ=255)
                                         cgctctgtggctgAGGTCAGACGGAGTGGTGGTGGCGGGATGCCGACCACGCCGGACGCCGATTGCGTGGAAAT‑TGCGGGTTCCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGa                                                                                             <  1:195068/134‑1 (MQ=255)
                                          aCTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGAt                                                                                            <  2:832790/143‑1 (MQ=255)
                                           cTTTGCTGCTGAGATCAAACAGAGTGATGGTGGGGACATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATc                                                                                           <  2:63130/143‑1 (MQ=255)
                                                 tgctgAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCcg                                                                                     >  2:4778/1‑143 (MQ=255)
                                                     gagaTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGtt                                                                                 >  2:67099/1‑143 (MQ=255)
                                                       gaTCGAACAGGTTGATGGTCGCGTTCTGGCGACCGGGCCGTACGCCGATGGCCTGGAAAT‑TGAGGGTTTCTGTTACGATGTAGATAGCGCGGCGGTTCCCGGTCGAGGCCTGTTGATAAACTTCTTTGATCAGCCCGCGTTCg                                                                               <  1:645986/143‑1 (MQ=255)
                                                        gtCAAACAGAGTGGTGGGGGCGTCATGACGTCCCCGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGa                                                                              <  1:119917/142‑1 (MQ=255)
                                                        aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAGAT‑TGCGGGGTGCGGCGAGGATGTAGATAGCGCGGCGGGCCCCGGTCGAGGCCTGCGGCGCCAGTTCTTGGAGCAGCGCCCGCTCGa                                                                              >  1:612159/1‑143 (MQ=255)
                                                        aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGa                                                                              >  2:871799/1‑143 (MQ=255)
                                                        aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGGTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTGCGa                                                                              >  1:457986/1‑143 (MQ=255)
                                                             aCAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGc                                                                         >  1:362632/1‑143 (MQ=255)
                                                                 agagagggaggCGTCAGGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGAc                                                                     <  2:232831/134‑1 (MQ=255)
                                                                       ggtggCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTaa                                                               <  2:832665/143‑1 (MQ=255)
                                                                         tggCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAAtt                                                             >  2:630660/1‑143 (MQ=255)
                                                                             gTCATGACCACCAAGCCGTAAGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTgg                                                         <  2:212398/143‑1 (MQ=255)
                                                                                  gacgacCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTCATTTTGCTTCCg                                                    >  2:312464/1‑143 (MQ=255)
                                                                                        cAAGCCGTACGCCGATTGCATGGAAAT‑TGCGGGCTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCCGAGGAGGCCTGCTGATCAACTCCCCTGATCAGCCCCCGGGCGGCAAGCAGACGCATACTTCTGGCTACggccgcc                                              >  1:374379/1‑137 (MQ=255)
                                                                                          tgCCGTAGGCCGATTGGGTGGGCCT‑TGCGGGTTTCGGTGGCGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCggg                                            <  1:293146/142‑1 (MQ=255)
                                                                                                  gCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGCTCAGCCCGCGGTCGGTAAGCTGACGCGTAATTTGGGGTACGCTGGCGGGGGCCAggc                                    >  1:768836/1‑141 (MQ=255)
                                                                                                  gCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGAAGCt                                    >  2:546852/1‑143 (MQ=255)
                                                                                                      aTTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCt                                <  1:303053/143‑1 (MQ=255)
                                                                                                        tGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCgggggaatatacacgcc                              <  2:653311/143‑13 (MQ=255)
                                                                                                             ggAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGCCTTTGCTCg                         >  1:737016/1‑143 (MQ=255)
                                                                                                              gAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCCGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTCACGCTGGCGGGGGCAAGCTGGCTTTGCTCgg                        >  1:554827/1‑143 (MQ=255)
                                                                                                                 ct‑tGCGGGTTTCGGTGGCGATGTAGATGGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTTGCTTTGCTCGGCaa                     <  2:85048/142‑1 (MQ=255)
                                                                                                                            tCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTAAATCcg           <  2:888023/143‑1 (MQ=255)
                                                                                                                                   cGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAtt    >  1:798748/1‑143 (MQ=255)
                                                                                                                                     aTGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATGCGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCACTCTGAATCCGCCAGATTgg  >  1:356873/1‑143 (MQ=255)
                                                                                                                                     aTGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTgg  >  1:70133/1‑143 (MQ=255)
                                                                                                                                        |                                                                                                                                           
TGCTGGGTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGG  >  NC_000913/701227‑701501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A