Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F9 I0 R1 142 103.7 2603848 81.8% 2129947 227.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 921,011 T→G 27.7% V222G (GTG→GGG)  macB → macrolide ABC transporter peremase/ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913921,0110TG27.7% 67.9 / 2.0 44V222G (GTG→GGG) macBmacrolide ABC transporter peremase/ATPase
Reads supporting (aligned to +/- strand):  ref base T (21/9);  new base G (6/8);  total (27/17)
Fisher's exact test for biased strand distribution p-value = 1.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGC‑TGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGC  >  NC_000913/920774‑921247
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               
cacaGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGTGGATGGCGATCCTGCATCACCTGCGCGATCGTGGGCATACGGTGCTTATCGTCACCCCAGAACTGCAGGTCGAGGCTCAGGCCGAGCAGGGGCTCGCACTTCACGACGGCGAAATtgtg                                                                                                                                                                                                                                                >  1:362874/1‑239 (MQ=255)
cacaGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGGGATCGAAATTCGCGCCGGCGAAATtgt                                                                                                                                                                                                                                                 >  2:607634/1‑238 (MQ=255)
cacaGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCCCGGGCCGAGCGGGGGATCGAAATTCGCGACGGCGAAATtgt                                                                                                                                                                                                                                                 >  2:62312/1‑238 (MQ=255)
 acaGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGGGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACAGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATtgtg                                                                                                                                                                                                                                                <  2:1200543/238‑1 (MQ=255)
  aaGCTTTCGGGTGGTCAGAAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTgcg                                                                                                                                                                                                                                              <  2:1139529/238‑1 (MQ=255)
    tgtttCGGGTGGTCAACAACAGGGCGCCAGCATCGGGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCa                                                                                                                                                                                                                                            <  2:688731/237‑1 (MQ=255)
       ttCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGAGAAGAGGTGATGGCGATCCTGCATCAGATGCGCGCTCGTGGCCATCCGGTGCTTATCGTACCCCACGATCCGCAGGCCGCTGCCGCGGCCAAGCGGGGGATCGAAAATCGCGACGGCGAAACTGGACGCaac                                                                                                                                                                                                                                          >  1:67794/1‑237 (MQ=255)
             tggtCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTccc                                                                                                                                                                                                                                    >  2:214155/1‑238 (MQ=255)
               gtCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGGGGGCATACGGTGATTATCGTCACCCACGGTCCGCCGGTCGCGGCTCATGCCGGGCGGGTGTTAGGAATTCGGCGCGGCACAATTTGGCGAAACCATCCCGc                                                                                                                                                                                                                                  >  1:168627/1‑238 (MQ=255)
                tCAGCAACAGCGGGTCAGCATCGGGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCa                                                                                                                                                                                                                                            <  2:126044/227‑1 (MQ=255)
                          cgcgTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCCCGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATCCGCGACGGCGAAATTCGGCGCAATCCCCCCGCCCTTGAAAAAgg                                                                                                                                                                                                                      >  1:1022366/1‑238 (MQ=255)
                          cgcgTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCCCAGGTCGCTGCTCCGGCCGAGCGGGTGATCGCAATTCGCGACGGCGAAATTGGGCGCAATCATCCGGCCCTTGAAAAAGt                                                                                                                                                                                                                      >  2:1261661/1‑239 (MQ=255)
                                                   tgaTCGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGACGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGCCGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGGCGGaa                                                                                                                                                                                               >  2:1011042/1‑236 (MQ=255)
                                                      aCGGTGGTCAGGTAATTCTTGCCGATGCACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATAGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAAGGGTACT‑GGCGGGACGGAACCggtt                                                                                                                                                                                         >  2:523551/1‑239 (MQ=255)
                                                      aCGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCTTTGAAAAAGGGAATGTTACT‑GGCGGGCGGGAACCtttt                                                                                                                                                                                         >  2:586288/1‑239 (MQ=255)
                                                      aCGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGGGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGGGCGTGTGATCCAAATTCGCGCCGGCGAAATTTGGCCCAACCCTCCCCCCATTGTAAAAGGGAAGGTTACT‑ggggggcggggacaaggg                                                                                                                                                                                         >  1:429988/1‑227 (MQ=255)
                                                      aCGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGCGATGGCGATCCTGCATCAGCTGCGCGCTCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGATCAGGCAGAGGGGGGGATCGAAATTGGCGACGGCGAAATTCGGCGCAAATCACCCGCCATTGAAAAAGGGAAGGGTACT‑ggggggggcagcaaggtg                                                                                                                                                                                         >  1:1141286/1‑227 (MQ=255)
                                                      aCGGTGGGCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGTCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCCGCTCCGCGATAGTGGGCATACGGGGATTATCGTCACCTAGCATCCGCAGGTCGCTCTTATGGCGGAGCGGCTGATCGCAATTGGCGAGTGCGACATTTTGCGAAATCCTCCAGCCCTGGCACAAGAGCATGGTACT‑gcatggcggcaccattgt                                                                                                                                                                                         >  1:313495/1‑222 (MQ=255)
                                                       cGGTGGTCAGGGAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCCTACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCCCGACGGCGACATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGTACTGAAccactt                                                                                                                                                                                         >  1:283119/1‑234 (MQ=255)
                                                         gggggcgggTAATTCTGCGCGATGAACCACCGGGCGCACTGGACAGCCATTCGGGCGAAGAGGTTATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCCCCCACGATCCGCAGGTCGCTGCTCAGGCCGGGCGGGTGATCGAAATTCGGGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCtgttg                                                                                                                                                                                        <  2:1022366/230‑1 (MQ=255)
                                                          tggtCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGGGAATGTTACT‑GGCGGGGCGGGACCCGTTGTcc                                                                                                                                                                                     >  2:1173306/1‑238 (MQ=255)
                                                            gtCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCTCGACGGCGAAATGGTGCGCAATCCGCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAAAACGTTGTCAac                                                                                                                                                                                   >  2:600347/1‑239 (MQ=255)
                                                                    aTTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGGAACcgttgtgacacaggggcc                                                                                                                                                                           >  2:200494/1‑227 (MQ=255)
                                                                                                     aGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACCCGGTGGCTGGCTGGGGGCA‑GTTTGTTATCGGTTTTAAcca                                                                                                                                         >  2:1258321/1‑237 (MQ=255)
                                                                                                          ttCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACAAGGCGTTCGGCTGGCGGCA‑GTTTTTccagggatttaaaagggcgc                                                                                                                                    >  2:570280/1‑220 (MQ=255)
                                                                                                            cTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCCCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCCGTTTTCAACACGGGTCCTGTCCTGCGGCATGTTTG‑CCGCGGGTTTAACGCGgggcgg                                                                                                                                  >  2:810333/1‑237 (MQ=255)
                                                                                                                         gATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGGACCTGTTGTCAACACGGGGTCTGGCTGGCGGCA‑GATTGTCACCGGGTTTAACGAGGCGGTggacatggactggc                                                                                                                     >  2:1056989/1‑226 (MQ=255)
                                                                                                                                 tCCTGCATCAGTTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCCGGTCGCTGCTCAGGCCGAGCGGGGGATCGAAATTCGCGACGTCGAAATTGTGTGCAATCCTCCCGCCCTTGAAAAAGTGAATGTTACTGGGGGGGAGGGAACCGGTGCTC‑ACAAGGCGTCTTGTTGCCTGCA‑GTTTTTCCGCGGGTTTTACAAGgcgccgcccagagcgcgggggggggcg                                                                                                             >  1:49101/1‑216 (MQ=255)
                                                                                                                                 tCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GCTTGTCAGCGGTTTTAACGGGGGCATGACGAAGGCCTggcggggcgtg                                                                                                             >  2:401348/1‑234 (MQ=255)
                                                                                                                                 tCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAAAAAGGGGTCTGGCTGGTGGCA‑GTTTTTCAGCGGTTTTAACGAGGCGCGGACGCTGGCATGGCGGgggcgg                                                                                                             >  2:367255/1‑237 (MQ=255)
                                                                                                                                            cTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGGACCCGATTGCACACCGGGTTTTGGCTGGGGGCC‑GATTGGCAGCGGGTGTATCGCGGCGCTGCCGGGGGCATGGGGGGGGCggggggggcgaaa                                                                                                  >  1:202151/1‑229 (MQ=255)
                                                                                                                                            cTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGGTAAAACGGGGTCTGGCTGGCGGCA‑GTTTTTCAGCGGTTTTAACGAGGCGCGGACGATGGACTGGCGGGGGCTGGGAGCGAATaa                                                                                                  >  2:737234/1‑239 (MQ=255)
                                                                                                                                                ggggtCGGGGGCATACGGTATTTATCGTCACCCAGGATCCGAAGGTCGTGGTCCAGGCGGGGGGGGTGACAGAAATTCGCGACGGCGAAATTGTGCGAAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAAt                                                                                               <  2:1245624/234‑1 (MQ=255)
                                                                                                                                                     ggggggCATAGGGTAATTTTGCCCCCCCACGACCCGCAGGGCGCTGCTCAGGCCGGGGGGGTGATCGAAATTCGCGACGGCAAAATTGGGCCCAACCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTa                                                                                          <  2:1246767/235‑1 (MQ=255)
                                                                                                                                                              ggcgTGATTCACCTCACCCACGGGCGGGGGGCCGCTGCTCCGGCCGGGCGGGTGATCGAAAGTCGGGCCGGCGAAATTGGGCGAAACCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGAc                                                                                <  2:195068/236‑1 (MQ=255)
                                                                                                                                                                     ttttCGTCACCCACGATCCGGAGGTCGCTGCACAGGCCGAGGGGGTGAACGAAATGCGCGACGGCAAAATTGGGCACTATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGC‑t                                                                         <  2:168627/235‑1 (MQ=255)
                                                                                                                                                                     ttggcctcccccGCGATCCGCAGGTCGCGGCTCAGGCCGAGCGGGTGATCAAAATGCGGGACGGCGAAATTGTGCGCAATCCCCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGC‑t                                                                         <  2:463691/229‑1 (MQ=255)
                                                                                                                                                                         gctCACCCGCGTCCCGCAGGTCCCTGCTCAGGCCGGGCGGGTGATCGAAATTCGCGACGGCGAAATTGGGCACAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGC‑TGGGGa                                                                    <  2:473030/237‑1 (MQ=255)
                                                                                                                                                                                cccGATCGCCAGGGCGCTTGTCAGGCCTAGCGGGTTAACTAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGTAGCGAATAAAATGCGTACTTTACTGACCATGC‑TGGGGATtattat                                                             <  2:615522/237‑1 (MQ=255)
                                                                                                                                                                                cagagtcgggagtgcGCTGCCGAGGCCGGGCGGGGGATCGAAATCGGGGGCGGCGAAAGTGTGCCCAGTCCGCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGC‑TGGGGATtattat                                                             <  2:1167596/225‑1 (MQ=255)
                                                                                                                                                                                caagATCCGCGGGGCGCTGCTGAGGCCGGGGGGGTGCTGAAAATTCGGGACGGGGAAATTGGGCGAAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGC‑TGGGGATtattat                                                             <  2:362874/236‑1 (MQ=255)
                                                                                                                                                                                      cggagggccggtgccCAGGCCGGGGGGGTGATCGAAATTCGCGCCGGCGAATTTGGGCGCAATCCGCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGC‑TGGGGATTATTATCGGTAt                                                       <  2:899032/228‑1 (MQ=255)
                                                                                                                                                                                        gCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCC‑GTTTGTCAGCGGGTTTAACGAGGCGCTGACGATGGCATGGCGGGGGCTGGCGGCGCATAAAAAGCGTGCTTTTCTTACCCTGC‑TGGGGGTTATTATCGGGAttt                                                     >  2:353159/1‑238 (MQ=255)
                                                                                                                                                                                               gctgctCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGa                                                                                                                                                                                                    >  1:992867/1‑91 (MQ=255)
                                                                                                                                                                                               gctgctCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGa                                                                                                                                                                                                    <  2:992867/91‑1 (MQ=255)
                                                                                                                                                                                                            ggggggTGATCAAAATTCGGGACGGCAAAATGGGCCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGC‑TGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGt                                  <  2:66167/235‑1 (MQ=255)
                                                                                                                                                                                                             gCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCCGGCAGGGGATAAAAAGGGGCCTTTTCTGACCCTGCTTGGGGTTTTTTTTCGGATTTGGCTGGGGGGTTTcatttttg                                 >  2:934925/1‑232 (MQ=255)
                                                                                                                                                                                                                             cgggtcgtggAAATTGGTCGAAATCCTCCCGGCATTGAAAAAGTGAAGGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGC‑TGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGAcgccgc                <  2:49101/230‑1 (MQ=255)
                                                                                                                                                                                                                                           tgtgCACAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGGCAACGCGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGC‑TGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGc  <  2:49246/239‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               
CACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACT‑GGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGC‑TGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGC  >  NC_000913/920774‑921247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A