Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F28 I0 R1
|
54 |
59.4 |
1320120 |
91.9% |
1213190 |
218.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
2,149,958 |
T→C |
34.7% |
E343G (GAG→GGG) |
yegI ← |
protein kinase‑related putative non‑specific DNA‑binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,149,958 | 0 | T | C | 34.7%
| 30.8
/ 3.3
| 25 | E343G (GAG→GGG) | yegI | protein kinase‑related putative non‑specific DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (3/13); new base C (7/2); total (10/15) |
| Fisher's exact test for biased strand distribution p-value = 8.95e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACAT > NC_000913/2149739‑2150163
|
cgcgGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCCACTGTCGCCCTCACCACCGCCCCAACTTTCGCCAGCCCCAAATTCCCG‑CCGGCGGTTATAACCCCCTCGccc > 2:486493/1‑227 (MQ=255)
gcgtGAAGGCCGAAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACGGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATa < 2:20122/225‑1 (MQ=255)
ccTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGGGCCACTGGCGCCCTCACCATCGCCCCGAACTTTGCCCGACCAAAATTTCCC‑CCGGGGGGAATGACCCCTTccccccgttcacta > 2:633893/1‑217 (MQ=255)
taaaGGCCGAAAAGCAGCAGCAAAAATGCTGAAAGGGGGTTCTAAAGCAAAAGGATGATTCCGCGCCGAAACAGGCCTAA‑AGGAAGGGGCCTGCCAGGCGGTTGGAAATGATCGGTTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCAAAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATaaa < 2:137902/225‑1 (MQ=255)
cagcGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCCACTGACCCCATCACCATCGCCCCGACTTTCGCCAGCCACAAATTCCCC‑CCCGGGGGAAGGACCCCCTTCCCGCGACCAATAAAATAAATAaaacacttata > 1:659438/1‑221 (MQ=255)
aGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATTACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCC‑CCGGTGGGAATGACCTCTTCCCCGGGATCAATAAAAAAGGAAAAACCtgtctcttttacaaatttccga > 2:9306/1‑207 (MQ=255)
aGCAGAATGATGTATTCGCGCCGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGGGCTACCTACGCCATCACCAACCCCCAGACTTTCACCCGCACAAAATTTCCG‑CCGGGGGGAATGACCCCTTCGCCCGGATCAATAAAAAAGATAAAACCCTTATTTTTCAGCCCAcacccacggcagacaagg > 1:655252/1‑214 (MQ=255)
tgatgTATTCGCGCCGTAACAGGCCTAA‑GGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTGGCAATGCTGGCGGTGCTACGGACGCCATCACCATCGCCCAGACTTTCGCCAGCAAAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAg < 2:86614/228‑1 (MQ=255)
tgaggTTTTCGCGCCTTAACGGGCGTAA‑AGGAAGCGGCCGGCCAGTCGGTTGGAAAGGATCGGGTAATGGCAGTTGCAATGGTGGCGGTGCTACGGACGCCACCACCATCGCCCAGACTTTCGCCAGCAAAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAg < 2:127331/228‑1 (MQ=255)
tgagtgttcagagcGTAAAAGGCCCAAGAGGGAGCGCCCTGCCAGGGGGTGGAAAATGACGGGGTAATGGCATTTGCGATGTGGGCGGTGCTCCTGACCCCATCACCCCCGCCAGGTCTTCCGCCAGCAAAAATTTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAg < 1:372019/215‑1 (MQ=255)
ggttttgtggcgcCGTAACAGGCCTAA‑AGGAAGCGGCCGGCCAGTCGTTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGGGGTGCTACTGACGCCATCACCATCGCCCAGACTTCCGCCAGCACAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGc < 2:167344/219‑1 (MQ=255)
ccGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCCTCGCCCAGACCTTTGCCCGCACAAAAATCCCG‑CCCGGGGGAATGACCTCTTCGCCCAGATCAATAAAAAAAGAAAAACCCTGTTTTACCAGCGCACACCACCGGGCGA‑CCATCAAAAAAGCGGGGGAAACaac > 1:565792/1‑226 (MQ=255)
tAACAGGCCAAA‑AGGAAGCGGCCTGCCGGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATaaa < 2:354793/159‑1 (MQ=255)
gacggggCCTGCCGGTCGGTTGGAAATGATCGGGAAAGGGCAGTTGAAAGGCTGGGGGTGCAACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATAc < 1:216940/223‑1 (MQ=255)
gcgcgccctgcgtggggTTGGAAATGACGGGGTAATGGCGGTGGCAAGGCTGTGGGGCCTACGGCCCCATTCACCATCCCCCAGACTGTCGCAGGAAAAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACAt < 1:633893/213‑1 (MQ=255)
aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGCGCTCCTGACCCCCCCCCCATCCCCCCAACATTCGCCCGCACAAAATTCCCC‑CCGGGGGGAATGACCCCCTCGCCGCGGTCAACAAAAAAAAAAAAACCCCGCTTATCCCGCCCCCCCCACCGGCAATCCAGACAAA‑GATCCGGGGAAACAAGCAATGcccacaacac > 1:136864/1‑215 (MQ=255)
tCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGAAGCCATCACCATCCCCCAGACGTTCGCCCGCACAAAAATTCCC‑CCGGTGGTAATGACCCCCTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCACCGCCCACCCCAGGCCGG‑CAGTCAAAAAAAAGGGGGAAAAATTAATTGCCGAAAACAAACATTTT‑TTTTAcatgtgggggagaaaagcc > 1:423868/1‑210 (MQ=255)
ggtgctgtgcggttacagtcgcggCTGTGCTATGGCCGCTATCCCCCTCGCCCAGACTTCCGCCAACAAAAATTCCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCa < 1:14099/205‑1 (MQ=255)
aGTTGCAAGGCTGGCGTTGCTACTGCCGCCATCACCATCGCCCAGACTGTCGCCAGCCAAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTAcc < 2:229356/228‑1 (MQ=255)
gCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCG‑CCGGGGGTAATGACCCCTTCGCCGAGGTCAAAAAAAAAAATAACACCTTGATTATCCCACGCCCACCACGGGCAGA‑CAGGCAAAAAAGCGGGGGAAAAATGCCTTGCTGAAACGGTAACTTTCTTTTAATTTGTGGCTAAGAGAGTCC‑TCGCGGCACCCCACCCTTTAGCCCTCgg > 1:375351/1‑227 (MQ=255)
tCGCCAGCACAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCTTGaagt > 2:349581/1‑226 (MQ=255)
aaaTTCCCG‑CCGGTGGTAATGACCCCTTCGCCGAGATCAATAAAATAGATAACACCGTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAAc < 2:136864/228‑1 (MQ=255)
aaatCCCGCCCGGTGGTAATGACCTCTTAGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAAc < 2:655252/225‑1 (MQ=255)
aTTCCCG‑GCGGTGGTAATGACCCTTGCGGCGAGATCAATAAAATAGATAACCACTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACGGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACAt < 2:295944/228‑1 (MQ=255)
aTTCCCG‑GCGGGTGTATAGCCCTTCTCGCCGGGAACAATAAAATAGATAACCCCTGGATTAACCAGGGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACAAGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACAt < 1:486493/228‑1 (MQ=255)
|
CGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACAT > NC_000913/2149739‑2150163
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
