Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F17 I0 R1 52 59.5 1410130 88.0% 1240914 215.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,616,800 G→A 23.4% V346V (GTG→GTA)  yneK → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,616,8000GA23.4% 88.4 / 5.3 49V346V (GTG→GTA) yneKuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (27/10);  new base A (3/9);  total (30/19)
Fisher's exact test for biased strand distribution p-value = 5.46e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTAACCGTTTTAAGATCATTTCATCACTTTTTCGCAACTCACCCGATAA  >  NC_000913/1616574‑1617021
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                             
ttttAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACAGCC‑AAAAGTTACTCAC‑GAAGACCTGGTg                                                                                                                                                                                                                               >  2:38085/1‑227 (MQ=255)
         aaCAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGACAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTTAAACTGGTTGTAAAAAATGAAAAACAGCC‑AAAAGTTACCCAC‑CAAAACCTGGTGaaaaaaa                                                                                                                                                                                                                        >  1:565925/1‑223 (MQ=255)
               atttttctctTTTTCAGAACTTTTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTAGGAATCCAAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCt                                                                                                                                                                                                               <  1:122631/222‑1 (MQ=255)
                  ctcctcATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTccc                                                                                                                                                                                                            <  2:280392/228‑1 (MQ=255)
                      tcATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGt                                                                                                                                                                                                        <  2:154795/228‑1 (MQ=255)
                      tcATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAAAACCGCA‑AAAAGTTACTCAC‑GAAAACCTGGTAAAAAAAAAGCCTCAccccccag                                                                                                                                                                                                         >  1:280392/1‑225 (MQ=255)
                            taaGAATTTGTTTTTTTTTTTCGCAAATATTGTATTTCACTTGCACCAATTTAAGAACCCAAATGAAAGTGATTTGAATGACGTGTTGAGGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAAGGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGacc                                                                                                                                                                                                  <  1:38085/226‑1 (MQ=255)
                               gAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAAAACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAACCAGTCCCAGCTCCCCTTTCAGaccgc                                                                                                                                                                                                >  2:623933/1‑225 (MQ=255)
                                    tGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAAAACCGCC‑ACAAGTTACTCAC‑GACGACCTGGTGAAAAACAGAACTCGCTCCCCGTTCAGacaccataaac                                                                                                                                                                                          >  1:547681/1‑219 (MQ=255)
                                      tttttATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAAAACCGCC‑AAAATTTACTCAC‑GAAGCCCTGGTGAAAAACAGGCCTCGACACCCTGTCAGACCACCTATAacc                                                                                                                                                                                        >  2:248603/1‑228 (MQ=255)
                                                              ttGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCCCCCCTGTCAACCCCCCTAAACCCGCAAACCCCAAAACCCCAAAcccc                                                                                                                                                                >  1:453189/1‑226 (MQ=255)
                                                                gCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACACCCTATACCCGCAACCGCCAAAACGCCAGACCTccc                                                                                                                                                              >  2:523307/1‑228 (MQ=255)
                                                                 cACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCATACCTCCCt                                                                                                                                                             >  2:480162/1‑228 (MQ=255)
                                                                   cTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGCCCACCTATACCCGCAACCGCCAAAACGCCAAACCTCCCTg                                                                                                                                                            >  2:464212/1‑227 (MQ=255)
                                                                   cTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAACTTACTCAC‑GAAGACCTGGTAAAAAACAGGCCTCGCTCCCCTGTCAAACCACCTATACCCGCAACCCCAAAAACCCCAAAccccccca                                                                                                                                                            >  1:337619/1‑225 (MQ=255)
                                                                    tttCACCAATTTAAGAACCCAAAAGAAAGTAATTTAAATAACGTGTGGAGGCCAGCATCATTAAGGGATAATAAAATTATAAAAAATAGGGCGCGCAGGCCCCAAAAACTGTTTGAAAAAAATGAAACCCCGCC‑AAAAGTTACCCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGaa                                                                                                                                                          <  1:40520/226‑1 (MQ=255)
                                                                    tATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTcccc                                                                                                                                                                                                           <  2:73006/179‑1 (MQ=255)
                                                                     aTCACCAATTTATGAATCCCTATGAAAGTGATTTGAATGACGTGTTGATGCCAGAATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGAAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCG‑AAAAGTTACTTAC‑GAAGACCTGGTGAAAAACAGGCCTCGTTCCCCTGTATGACCACCTAAACCCGCAACCGCCACAACGCCAGACCTCCCTGAAc                                                                                                                                                         >  2:549653/1‑228 (MQ=255)
                                                                      tCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACg                                                                                                                                                        >  2:296351/1‑228 (MQ=255)
                                                                      tCACCAATTTATGAATCCCAATGAAAGGGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACAACCTATACCCGCAACCGCCAAAAAGCCAGACTTCCCTGAACg                                                                                                                                                        >  2:118596/1‑228 (MQ=255)
                                                                                          aagAAAGTTATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGAAAAAATTACCAACCATATGGCGCGAAGGCCCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATCCCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTAc                                                                                                                                    <  2:453189/226‑1 (MQ=255)
                                                                                           tGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAAGCCTCGCTCCCCTGTCAGACCACCAATACCCGCAACCGACAAAACGCCAGACCCCCCTGAACGTCATTAAACCGCGATGTTAcc                                                                                                                                   >  1:697654/1‑228 (MQ=255)
                                                                                               aGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGTCGCGCAGGACCCTCAAACTATTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTAAC‑GAAGACCGTGTAAAAAACATGCCTCGCTCCCCGGTCAGACCACCTATACCCGCAACCGCCAAAACACCAGACCCCCCTGAACGACATTAAAACGAAATGTTACCgccc                                                                                                                               >  2:257710/1‑225 (MQ=255)
                                                                                                   aTTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGAGGTTACCGActctct                                                                                                                           >  2:13422/1‑228 (MQ=255)
                                                                                                                 ttGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGACCTCGCTCCCCTGTCAGACCACCTATACCCGCAACAGCCAAAAAGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACACCCCCTCTAACGCGCGAAAGaa                                                                                                             >  2:311559/1‑228 (MQ=255)
                                                                                                                           catcatTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGa                                                                                                                                                                    >  2:444421/1‑163 (MQ=255)
                                                                                                                                        tgatAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCCTGATGTTACCGACACTCTGACCCCTGAAAGAATAAGAGCCCGAGAACCGGaaaa                                                                                       >  2:271461/1‑227 (MQ=255)
                                                                                                                                            aaaaTAATCAAACCTTGGCCACGCGAGACCCTCAAAATGTTGTAAAAAAAGGACCCACCCCC‑AAAAGTTACCCAC‑GAAACCCTGCTAAAAAACACGCCCCGCCCCCCTGTCACACCCCCTATACCCGCAACCGCCAAAACGCCAGGCCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGa                                                                                  <  1:686192/228‑1 (MQ=255)
                                                                                                                                                 tataaaaaattgggCCCGCAGCACCACAAACCTGTTTGAAAAAAATGAAACCCCGCA‑AAAAGTT‑CACACAGAAGACCTGGTAAAAAACAGGCCTCCCTCCCCTGTCCGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAg                                                                             <  2:337619/216‑1 (MQ=255)
                                                                                                                                                        catcgcccccaaaaccccccCAAATGGTTTGAAAAAAAACACACCCCCCC‑AAAACGACCACCC‑GAAGACCTGGTAAAAAACAGCCCTCCCCCCCCAGCCAAACCCCATATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGCCATTAAACCGCGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACaa                                                                      <  1:296351/212‑1 (MQ=255)
                                                                                                                                                             ggCGCGCAGGACCCTCAAACTGTTTGTACAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCCCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACCCCAGACCTCCCTGAACGCCATTAAACCGTGATCTTCCCGCCTCTCTGACGCGTTACAGAATCAGCGACGGAGAAACGGAAAACCCCGTCCAACTCACaaacaca                                                                 >  1:19101/1‑224 (MQ=255)
                                                                                                                                                             ggCGCGAAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACCCAC‑GAAGAACTGGGGAAAAAAAGGCCTCGACCCCCTGTCCGAACACCTCCCCCCGAAAACACCCCCCCGCCAGCCCTCCCTGAACGTCATTAAACACCGATGCTCCCGACCCTCTCACGCGGAAAACAACCCACCTCAGAAAAAAGCAAAACGAGAACCAGaaaccaaaacca                                                                 >  1:551222/1‑217 (MQ=255)
                                                                                                                                                             gccgcgcATGCCCCTCAAACTG‑TTGTAAAAAACCAAACCCCCCCAAAAAGTACCCCAC‑GAACCCCGGGAGAAAAACCGCCCGCGCCCCCCTGCCACACCTCCTACACCCGCAGCCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCa                                                                 <  1:480746/226‑1 (MQ=255)
                                                                                                                                                              gcgcgaggcaccccaaaaCTGTTAGAAAAAAATAAAACCCCCCC‑AAAAGTTACTCCA‑CAAGACCGGGTAAAAAACAGGCCTCGCTCCCCTGTAGGACCCCCTAACCCCGAAGCCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCAt                                                                <  1:547654/213‑1 (MQ=255)
                                                                                                                                                                 cgcAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGACAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACCCGGGCAAGAATCAGCGCCAGAGAACCGGAAAACGCGATCCAGATCACAAATGAATTga                                                             >  1:656890/1‑227 (MQ=255)
                                                                                                                                                                 cgcAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTACCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGCCATTAAACCGTGATGTTAcc                                                                                                                                   >  2:64236/1‑158 (MQ=255)
                                                                                                                                                                     ggcacctaaaccagttttaaaaaaaTGAAACCCCGCA‑AAAAGTTCCCCCC‑GAAGCCCTGGAAAAAAACAGGCCCCGCTCCCCTGTCAGCCCACCTATCCCGGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTc                                                         <  2:656890/209‑1 (MQ=255)
                                                                                                                                                                       ccccccaaacgtgttGAAAAAAATAAACCCCCCCC‑AAATGTTCCCCCC‑AAATGCCGGGCAAAAACCGGGCCGCCCCCCCCTGTAAACCCCCCTATACCCGAAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTcac                                                       <  1:464212/215‑1 (MQ=255)
                                                                                                                                                                        accaaaatctgttgtgaaaaaaCGAACCACCCCC‑AAAATTTACCACC‑GAAGCCCTGGTAAAAAACAGGCCTCGCACCCCTGGCAGCCCCCCTACGCCCGAAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCGCGGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTca                                                        <  1:89685/210‑1 (MQ=255)
                                                                                                                                                                               aaaaagagacagaaaTGAACCCCCGCC‑AAAAGTTACCCAC‑GAAGACCTGGTGAAAACCAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGAAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTaa                                               <  2:263939/216‑1 (MQ=255)
                                                                                                                                                                                cTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCAAGACCTCCCTGAACGCCATTAAACCGTTATGTTACCGACCCCCTGACGCGTGAAACAACAATCGTCACAGAACCGGAAAACCCGATCCAATTCACAAATACATAGCAttaaaaaaacaaac                                              >  1:699649/1‑216 (MQ=255)
                                                                                                                                                                                 tGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCCAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGCCGCGTGAAAGAATCAGCGTCCGAGAAACGGACAACGCCTTCCAGATCCCAAATGCATTGTATTCACATCATTAAcc                                             >  2:57487/1‑228 (MQ=255)
                                                                                                                                                                                  gTTTGTAAAAAATCAACCCCCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAACCAGGCCTCGCTCCCCTGTCAGCCCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTaa                                               <  2:482900/225‑1 (MQ=255)
                                                                                                                                                                                   tttGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGACCCAGATCACAAATGCATTGGATTCCCATCATTAACCGt                                           >  2:20018/1‑228 (MQ=255)
                                                                                                                                                                                   tttGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACCCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGGATTCACACCCTTAACCGt                                           >  2:451675/1‑228 (MQ=255)
                                                                                                                                                                                     tGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGt                                                                                                                                            >  2:415296/1‑129 (MQ=255)
                                                                                                                                                                                                      cGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAAAGCAATGAATTCACATCATTAACCGTTTTaaaacgatcccttaa                         >  1:263939/1‑214 (MQ=255)
                                                                                                                                                                                                        cc‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGCGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCCCATCATTAACCGGTTTAAGATAATTTCTACACtt                      >  1:53807/1‑228 (MQ=255)
                                                                                                                                                                                                                      cacaaaccccgggTAAAAACCGGGCCTGCCTCCCCTGTCAAACCACCAAACCCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAGATGCCTTGTTTTCCCATCATTAACCGTTTTAAGATCATTTCATCACTTTTTCGCAACTCa          <  2:551222/217‑1 (MQ=255)
                                                                                                                                                                                                                              ccTGGGAAAACACCGGCCTCGCTCCCCTGCCAGCCCCCCTATCCCCTCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTAACCGTTTTAAGATCATTTCATCACTTTTTCGCAACTCACCCGATaa  <  2:53807/228‑1 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                             
TTTTAGCAGAACAAGAATCTCCTCATTTTCAGAACTTGTTTTTATTTTTCGCCAATATGTTATTGCACTATCACCAATTTATGAATCCCAATGAAAGTGATTTGAATGACGTGTTGATGCCAGCATCATTAAGTGATGATAAAATTATCAAACATATGGCGCGCAGGACCCTCAAACTGTTTGTAAAAAATGAAACACCGCC‑AAAAGTTACTCAC‑GAAGACCTGGTGAAAAACAGGCCTCGCTCCCCTGTCAGACCACCTATACCCGCAACCGCCAAAACGCCAGACCTCCCTGAACGTCATTAAACCGTGATGTTACCGACTCTCTGACGCGTGAAAGAATCAGCGTCAGAGAAACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTAACCGTTTTAAGATCATTTCATCACTTTTTCGCAACTCACCCGATAA  >  NC_000913/1616574‑1617021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A