Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 726,177 A→C 13.1% V287G (GTG→GGG)  kdpB ← potassium translocating ATPase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913726,1770AC13.1% 291.6 / 2.3 126V287G (GTG→GGG) kdpBpotassium translocating ATPase, subunit B
Reads supporting (aligned to +/- strand):  ref base A (52/51);  new base C (5/16);  total (59/67)
Fisher's exact test for biased strand distribution p-value = 3.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

GCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACGCGGAAAACG‑GCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTCGTTCGGCGTTTTGCGTCGCTGTG  >  NC_000913/725948‑726408
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          
gcgcAGGTTAAAACGGTGCTTGGCGGGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCgcgc                                                                                                                                                                                                                                   <  1:55299/239‑1 (MQ=255)
        tAAAACGCTTCTTGGCGGGGTTCCACATCCTGGGGCCTTCCGGCGTTTCACCAGCCAG‑CGAAGCCAGTTGTGCGGGGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATc                                                                                                                                                                                                                           <  1:470444/239‑1 (MQ=255)
            ggttaaaaagacagggATCCCAATGCTGCGGCCTTCCGGGTTTTCCCCCGCCGG‑GGAAGCCAGTTGTGCGGGCGCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCgg                                                                                                                                                                                                                        <  2:91756/224‑1 (MQ=255)
            aCGGTGGTTGGCCGGGATCACAATCCGGGGGCCTTCCGGGTTTTCCACCGCCCG‑CGAAGCCAGTTTTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCGGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGc                                                                                                                                                                                                                       <  2:191436/239‑1 (MQ=255)
                tgctTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCCGCTCa                                                                                                                                                                                                                    >  1:38572/1‑238 (MQ=255)
                tgctTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCCCCCCTAGCATCCGGcccc                                                                                                                                                                                                                    >  2:1245736/1‑235 (MQ=255)
                tgctTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCAAATTCCCGCCTAGCATCCGGCTCa                                                                                                                                                                                                                    >  1:1098755/1‑238 (MQ=255)
                tgctCGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGACTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTGGCAGAACG‑CCACGTCCACCTGCCGCTTCACCGG‑AACGCCGCCTGGGGGAAATCACATTCGCCCCTacccaccccctca                                                                                                                                                                                                                    >  2:47605/1‑226 (MQ=255)
                 gctTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCACGCATCCGGCTCac                                                                                                                                                                                                                   >  1:666415/1‑237 (MQ=255)
                     ggCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCg                                                                                                                                                                                                               >  1:332362/1‑238 (MQ=255)
                     ggCGAGGATCAAAATTCTGGGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCgg                                                                                                                                                                                                              <  1:1095721/239‑1 (MQ=255)
                         gggtatcaattgcgggggCCTCCCGGGGTTTGTTCACCCGG‑CGTATCGCTTTGGGCGGCGTCCGCCTGCGTTTTTTCCTCCCGGCCCTGGGC‑GGGG‑ATAAACCCCGACGCCTGACGGTTACCGAGTGTA‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGa                                                                                                                                                                                                           <  2:584202/222‑1 (MQ=255)
                              caccatgctggcgCCTTCCTGCGTTTCATCACCCAG‑CGAGGCAGGGTGTGCGGCGTCAGCGGGCGTTTTTTCACCCCCGCCCGGCGC‑GGGG‑ATAAACTCCGACGCCGGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CCCCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGa                                                                                                                                                                                                     <  2:114576/227‑1 (MQ=255)
                              cacaATACTGGGGCCTTCCGGCGTTTCACAAGCCAG‑CGAAGCCAGTTGGGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGa                                                                                                                                                                                                     <  1:925060/239‑1 (MQ=255)
                              acaaaTACCGCGGCTTTCCGTCGTTTCCCCCGCCAGTCGAGCCCTGTGGT‑CGGCGGCCGCCAGCGTTTTTTCACCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGACTGCCGGTTCCCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGGAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGa                                                                                                                                                                                                     <  2:460390/236‑1 (MQ=255)
                                caATACTGCGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAACCACATTCGCCCCTAGCATCCGCCCCACCCCgcggccccgcgcc                                                                                                                                                                                                   >  2:1057457/1‑227 (MQ=255)
                                   ttcTGCGGCCTTACGGGGTTTCACCAGCCAG‑CGAAGCCAGTTGTGCGGGCTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCt                                                                                                                                                                                                <  1:220631/237‑1 (MQ=255)
                                      tGCGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCgg                                                                                                                                                                                                              >  1:91756/1‑222 (MQ=255)
                                        cGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCCGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGCCGTTTACCGAGGGTG‑ATGGCGCCGG‑TTTTCTCCAGCAGCAGAACGTCACCGT‑CACCTGCCGCTTTAACTG‑CACGCCCGCTGGGGGCCATCACATTCGCGCCTCGCATCCCGCCCATCCCCGCGACGCCCATCCCCGaca                                                                                                                                                                                            >  2:704405/1‑238 (MQ=255)
                                        cGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGCCGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CCCCGGCCGCTTCACCTG‑CCCGTCCGCTGGTGGCAATACCATTCGCGCCTACCACCCGCATCACCCCgccacgcccgatcccctaaaa                                                                                                                                                                                           >  2:790522/1‑219 (MQ=255)
                                        cGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCACCTG‑CACGTCCGCTGGTGGCAATCACATTCGCCCCTCGACTCCGGCCCATCCCGGCGCCCCCGCTCGCTGaca                                                                                                                                                                                            >  2:839124/1‑238 (MQ=255)
                                        cGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCCCATTCGCGCCTAGCATCCGGCTCATCCCGCCGACGCCCATCGCTGacaa                                                                                                                                                                                           >  2:465012/1‑239 (MQ=255)
                                        cGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGTTcccggccac                                                                                                                                                                                           >  1:1230142/1‑231 (MQ=255)
                                        cGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGcca                                                                                                                                                                                            >  1:803444/1‑238 (MQ=255)
                                        cGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGcca                                                                                                                                                                                            >  1:933353/1‑238 (MQ=255)
                                        cGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGcca                                                                                                                                                                                            >  1:1068227/1‑238 (MQ=255)
                                        cGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGaca                                                                                                                                                                                            >  1:344880/1‑238 (MQ=255)
                                        cGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCACCCACGCGACGCCGATCGCTGccaa                                                                                                                                                                                           >  1:477136/1‑239 (MQ=255)
                                        cGGCCTTACGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGGTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGAAGCAGAACGTCAACGT‑AACCTGCCGCTTCAACTG‑CACGTCCGCTGGGAGCAATCACATTCGCGCCTGGCATCCGGCCCATCCCGGGGAGGCCGATCGCTGacaa                                                                                                                                                                                           >  2:253816/1‑239 (MQ=255)
                                         ggCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCCTCCGGCGCATCCCGGCGAGGCCGATCACTGacaac                                                                                                                                                                                          >  1:1322007/1‑239 (MQ=255)
                                          gCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CCCCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCCCATTCGCGCCTAGCATCCGGCTCATCCCGCCCACGCCGATcccggaaaaaa                                                                                                                                                                                         >  2:225272/1‑229 (MQ=255)
                                           ccTTCCGGTGTTTCATCAGCCAG‑CGAAGCAAGTTGGGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTAT‑CAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAg                                                                                                                                                                                        <  1:1220605/238‑1 (MQ=255)
                                           ccTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTCCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTCGCGGCAATCTAATTCGCGCCAGCCATCCGGCCCATCCCCGccgccccaaccccagacaaagg                                                                                                                                                                                        >  2:1159420/1‑218 (MQ=255)
                                             ttCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCCGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCCAATTCGCGCCTAGCATCCGGCCACTCCCGGCGCCGCCGCGCGGTGACAACAga                                                                                                                                                                                       >  1:1183694/1‑237 (MQ=255)
                                              tcgggCGTTTCACCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGgcc                                                                                                                                                                                     <  1:1031890/236‑1 (MQ=255)
                                               ccGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTAATCCCGGCGACGCCGATCGCTGACAACAGgcc                                                                                                                                                                                     >  1:380133/1‑238 (MQ=255)
                                                 gggttttctctgcgcgg‑cGAGGCCCGTTTGGGGGCGTCAGCCGGGGTTTTTTCATCCCCGCCCTGCGC‑GGGG‑ATAAAACCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCATTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGgccgcc                                                                                                                                                                                  <  2:1171392/223‑1 (MQ=255)
                                                 ggCGTTTCACCACCCAG‑CGAAGCCCGGTGTGCGGCGTCAGGCGTGTTTTTTTCATCCAGCGCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGGG‑AGGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGgccgcc                                                                                                                                                                                  <  2:666415/239‑1 (MQ=255)
                                                     tttCATCAGCCAG‑CGAAGCCAGTTGTGCGGCTTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATa                                                                                                                                                                              <  1:486564/239‑1 (MQ=255)
                                                      ttCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATa                                                                                                                                                                              <  1:1245736/238‑1 (MQ=255)
                                                       tcatcaGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGCCAACAGGCCGCCAATAGt                                                                                                                                                                            >  1:26772/1‑239 (MQ=255)
                                                          tcaGCCAG‑CGAAGCCAGTTGGGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGt                                                                                                                                                                         <  1:791651/239‑1 (MQ=255)
                                                          gcagccag‑gGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCCCGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGt                                                                                                                                                                         <  1:1068926/238‑1 (MQ=255)
                                                          ccccccag‑gGAACCCAGTTGGGCGGCGTCGGCCAGGGTTTTTTCACCCACGCCCTGCGC‑GGGG‑AAAAACTCCGACGCCTGACGGTTACCGGGTTTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGACCGCCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGt                                                                                                                                                                         <  2:933353/235‑1 (MQ=255)
                                                                     gaGCCTGTTGGGCGCGGTCCGCCAGGGTTTTTTCATCCGCGCCCTGGGG‑GGGG‑ATAACCTCCGCCGCCTGACGGTTACCAAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGACCGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATcaga                                                                                                                                                               <  2:297614/238‑1 (MQ=255)
                                                                      aGCCAGTTGGGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATcaga                                                                                                                                                               <  1:225272/238‑1 (MQ=255)
                                                                            ttGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACca                                                                                                                                                        >  1:1374221/1‑239 (MQ=255)
                                                                             ttgtcggccgcgcccgtgtttttttCCCCCTCGCCCTGCGC‑GGGG‑ATAACCCCCGACGCCTGACGGTTCCCGGGTGTG‑ATGGGTCCGT‑TTTTATCCAGTAGCAGAACGTCACCGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACcag                                                                                                                                                       <  2:680907/220‑1 (MQ=255)
                                                                               tgCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGGGGTTGGGATCAGACAGACcagca                                                                                                                                                     >  1:982122/1‑239 (MQ=255)
                                                                                    cgTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGCCAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGcc                                                                                                                                                >  1:680907/1‑239 (MQ=255)
                                                                                    cgTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGCGGACGCCGATCGCTGACAACAGGCCGCCAATATTGGTTGGGATCAGCCAGACCAGCAACGcc                                                                                                                                                >  1:587593/1‑239 (MQ=255)
                                                                                    cctcctcctcctttttttCA‑CCCCCCCCCGCGCGGGGG‑ATAAACCCCGACCCCTGATGGTTCCCGGGTGTT‑ATGGTGCCGG‑TTTTAACCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACGG‑CCCGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGcc                                                                                                                                                <  2:477136/227‑1 (MQ=255)
                                                                                      tCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGCCAACAGGCCCCCAATAGTGGTTTGGagccgaacgacccacacggccc                                                                                                                                               >  2:1144244/1‑217 (MQ=255)
                                                                                      ccccccccggTTTTTTCACCCACCCCCCGGGC‑GGGG‑AAAACCCCCGACGCCTGAGTGTTCCCGTGTGTG‑ATGGTGCCTG‑TTTTCTCCAGTGGCAGAACTTCACCGT‑CCCCTGCCGCTTCAACCG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGccac                                                                                                                                              <  2:1082314/230‑1 (MQ=255)
                                                                                          gccccGTTTTTTTACTCACGCCTTGTGC‑GGGGAATAACCTCAGGCTCCTACCGGCTACCGAG‑GTG‑GTTGGGCCGGTTTTTATCCAGAAGCAGAACGCCACCGT‑CTCCTGCCGCGTGAACTG‑CACGGCCGCTGTGGGCAATCCCATTCGCGCCTAGCAACCGGCTCATCCCGCCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCcagcag                                                                                                                                           <  2:501902/235‑1 (MQ=255)
                                                                                           cgggtttttttCCTCCCGCCCCGGGGC‑GGGG‑TTAAACTCCGACGCCTGACGGTTCCCGTG‑GTGTATGGTCCCGG‑TTTTATCCAGTAGAAGAACGTCAACGT‑AACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTa                                                                                                                                         <  2:950878/234‑1 (MQ=255)
                                                                                             gcGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATAGCTGACAACAGGCCGCCCATAGTGGTTGGGTTCAGACCGACCAGCAGCGCCACCAgca                                                                                                                                         >  1:822864/1‑235 (MQ=255)
                                                                                                   tttCATCCCCCCCCTGCGC‑GGGG‑ATAAACCCCGCCCCCTGGCGGTTACCGGGTGTG‑ATGGGGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑Cg                                                                                                                                <  2:1230142/239‑1 (MQ=255)
                                                                                                   tttCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑Cg                                                                                                                                <  1:1061963/239‑1 (MQ=255)
                                                                                                     ttcccgtcGCCCTGGGG‑GGGG‑ATAAACCCCGTCGCCGACCGGTCACGGTGGTTT‑ATGGGCGCGG‑TTTTTTCCAGTGACCGACCGGCACCGT‑CACCTGCCGCTTCAACTG‑CACGTCCGGTGGTGGCAATCCCATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCt                                                                                                                              <  2:1028604/232‑1 (MQ=255)
                                                                                                     tcctCCACGCCCTGCGC‑GGGG‑TGAAACTCCGACGCCTAACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCt                                                                                                                              <  1:506847/236‑1 (MQ=255)
                                                                                                     tCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTCCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCCCATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCt                                                                                                                              <  1:1212682/239‑1 (MQ=255)
                                                                                                     tCACCCACGCCCTGCGC‑GGGG‑ATAACCCCCGACGGCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCt                                                                                                                              <  1:481854/239‑1 (MQ=255)
                                                                                                      tctcccccgccTGGGG‑GGGG‑GTAAACCCCGGCGTCTGAGGGTTACCGAGTGTG‑GTGGTGCCGG‑TTTTACCCAGTATCAGAACGTCAACGT‑CCCCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACAGTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTg                                                                                                                             <  2:1098755/230‑1 (MQ=255)
                                                                                                      cacccACGCCCTGCCG‑GGGG‑ATAAACCCCGACGCCTGGCGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCACCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTg                                                                                                                             <  1:996914/236‑1 (MQ=255)
                                                                                                       tcctccccccccgtgggggggtaaaaCCCCGCCGCTGGACGGTTCCCGGGTGTG‑GTGGTGCGGG‑TTTTACCCGGTAGGAGAACGTCAACGT‑CCCCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCAGCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCt                                                                                                                              <  2:1100421/216‑1 (MQ=255)
                                                                                                          ccgccccGGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTCCCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CCCCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTg                                                                                                                         <  1:1288540/235‑1 (MQ=255)
                                                                                                            cGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACACCAGGCCGCCAATAGTGGTTGGGGTCAAACAAACCACCCGCGCACCCAGAACCGTTA‑ccccgcagcca                                                                                                                       >  2:62641/1‑229 (MQ=255)
                                                                                                               ccTGGGG‑GGGG‑TAAAACTCCGACGGCTGGCGGTTACCGGGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAAAACGTCAACGT‑CCCCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTa                                                                                                                   <  1:894594/239‑1 (MQ=255)
                                                                                                                   cgc‑gGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTC‑CATTACCg                                                                                                                >  1:908985/1‑238 (MQ=255)
                                                                                                                    gcggggg‑ATAAACTCCGACGCCTGACGGTTACCGAGTTTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCg                                                                                                                <  1:1057457/235‑1 (MQ=255)
                                                                                                                     c‑gGGG‑ATAAACTCCGGCGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGcc                                                                                                              <  1:1159420/238‑1 (MQ=255)
                                                                                                                                  tCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACGCGGaaaa                                                                                                  <  1:1010093/239‑1 (MQ=255)
                                                                                                                                    cGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGCGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCACCCCGGaaaaa                                                                                                 >  1:1278278/1‑237 (MQ=255)
                                                                                                                                      aCGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTCCCGCCCCACGCGGAAAACg‑g                                                                                              >  1:1070091/1‑238 (MQ=255)
                                                                                                                                      aCGCCTGACGGTTACCGAGAGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCCAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGCCAACAGCCCGCCAATAGTGGTTGGGACCAGACATGACAGCAGCCACCCCAGAACCGTTA‑GACTGAACG‑CAATTCCGCCCCAccagaaaaaggg                                                                                               >  2:1231376/1‑227 (MQ=255)
                                                                                                                                            gACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGCCAACAGGCCGCCCATAGGGGTTGGGATCCGGCAAACCAGCCG‑GCCCACAGGACCCCTACCGCT‑ACTGCCATCACCCCCCCACCCGCAAAACGCGCcacac                                                                                        >  2:62537/1‑238 (MQ=255)
                                                                                                                                            gACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTCCCGTTA‑CGCGGACTG‑CATTACCCCCCCCCCCGGAAAACG‑Gcccaccc                                                                                       >  2:1010093/1‑234 (MQ=255)
                                                                                                                                                   aCCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGTCAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACCCGGAAACCG‑GCCCCAGCGTGGCgg                                                                                >  1:29029/1‑239 (MQ=255)
                                                                                                                                                     cggggttgtgttgTGCCGT‑TTTTTTCCAGTACGAGAACCGCACCGT‑CACCCGCCGCTTCAACTG‑CCCGTCCGCTGGTGGCAATCCCATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACGCGGAAAACG‑GCCACAGCGTGGCGGt                                                                               <  2:803444/227‑1 (MQ=255)
                                                                                                                                                       gatgtg‑ATGGGGCCGG‑TTTTACCCGTGAGCAGAACGGCACCGT‑CCCCTGCCGCTTCAACTG‑CCCGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACGCGGAAAACG‑GCCACAGCGTGGCGGTTGc                                                                            <  1:580394/237‑1 (MQ=255)
                                                                                                                                                       aGTGTG‑ATTGGGCCGG‑TTTTATCCAGTAGCAGAACGCCAACGT‑CACCTGCCGCTTCAATAT‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCAt                                                                                                                                                                                                                            <  1:1310761/98‑1 (MQ=255)
                                                                                                                                                       aGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGACTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCAATTAGCGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGATA‑CGCTGCCTG‑CATTCACCCCCCACGCCGAAAACG‑Gcacaaccagggagggggt                                                                            >  2:194713/1‑222 (MQ=255)
                                                                                                                                                           tg‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCCGTACCGTTA‑CGCTGACTG‑CATTAACGCCCCCCGCGGAAAACG‑GCCCCCCCGcggcggggggcgcc                                                                        >  2:157862/1‑231 (MQ=255)
                                                                                                                                                                tggtccGT‑TTTTCCCCAGTGGAAAAACGCCACCTT‑CCCCGCCCGCTTCAACTG‑CCCGCCCGCTGGGGGCCATCACATTCGCTCCGAGCATCCGGCTCATCCCGGCGACGCAGACCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACGCGGAAAACG‑GCCACAGCGTGGCGGTTGCCAGTaaaa                                                                    <  2:1032204/235‑1 (MQ=255)
                                                                                                                                                                 gTGCCGG‑TTTTCTCCAGTAGCAGAACGTAACCGT‑ACACTGCCGCTTCAACTG‑CACGGCCGCTGGTGGCAATCCCATTCGGGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGCCCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACGCGGAAAACG‑GCCACAGCGTGGCGGTTGCCAGTaaaa                                                                    <  1:1326722/238‑1 (MQ=255)
                                                                                                                                                                  tGCCGG‑ATTTATCCAGTAGCAGACCGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTACGCTGGTGGCAATCCCATTCGCGCCTAGCATCCGGCTCATc                                                                                                                                                                                                                  >  2:1310761/1‑98 (MQ=255)
                                                                                                                                                                  ggtccgtgtttACCCAGTAGCAAAACGGCACCGT‑CCCCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACGCGGAAAACG‑GCCACAGCGTGGCGGTTGCCAGTaaaa                                                                    <  1:1191074/230‑1 (MQ=255)
                                                                                                                                                                     cGG‑TTTTATCCAGTAGCAGAACGTAAACGT‑CCCCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACGCGGAAAACG‑GCCACAGCGTGGCGGTTGCCAGTAAAAAGACg                                                               <  1:62641/239‑1 (MQ=255)
                                                                                                                                                                          ttttcccggTAGCAGAACGTCAACGT‑CCCCTGCCGCTTCAACGT‑CACGTCCGCTGGTGGCCATCCCATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACGCGGAAAACG‑GCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAg                                                           <  2:982122/231‑1 (MQ=255)
                                                                                                                                                                                  gtcacAGAACGTCAACGT‑CACCTGCCGTTTCAACTG‑CACGTCCGCTGGTGGCAATCCCATGCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACGCGGAAAACG‑GCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCa                                                   <  1:1144244/235‑1 (MQ=255)
                                                                                                                                                                                  gTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCCCGCGGAAAACG‑GCCACAGCCGGGGGGGTGCCCGCaaaaaaaaaaaaaggaaggga                                                   >  2:1054183/1‑223 (MQ=255)
                                                                                                                                                                                   tAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCt                                                                                                                                                                                                >  1:275670/1‑100 (MQ=255)
                                                                                                                                                                                   tAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCt                                                                                                                                                                                                <  2:275670/100‑1 (MQ=255)
                                                                                                                                                                                   tAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCACTAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGAACCGTTA‑CGCTGCCTG‑CATTACCGCCCCCCGCGGAAAACG‑GCCACAGCGGGGGGGGGGCCAACaaaaaaaaaaaaagcagaggga                                                  >  2:659499/1‑222 (MQ=255)
                                                                                                                                                                                   tAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACGCGGAAAACG‑GCCACAGCGCGGCGGGTGCCCGCAAAAAAAAGAAAGTACGGGCaa                                                  >  1:1078714/1‑239 (MQ=255)
                                                                                                                                                                                                 cgctgaCCTGCCG‑TTCCACTGCCACTGCCGCTGGTGGCAATCCCATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACGCGGAAAACG‑GCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGt                                     <  1:728631/234‑1 (MQ=255)
                                                                                                                                                                                                 acggccccTGCCCGTTCAACTG‑GCCGTCCGCTGGTGGCAATCACCTTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACGCGGAAAACG‑GCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGt                                     <  1:286150/233‑1 (MQ=255)
                                                                                                                                                                                                      cccTGCCGCTTCAACTG‑CACGGCCGCTGGTGGCAATCCCATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACGCGGAAAACG‑GCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAggg                                <  1:218633/238‑1 (MQ=255)
                                                                                                                                                                                                                             ccccggttggCAATCCCATTCCCGCCTGGCTCCCGGCTCATCCCGGCGACGCCGATCCGTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGGCCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTCCCGCCCCACGCGGAAAACG‑GCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAAGCAGCAGAATGGTCAGGTCAAGTTCGTTCGGCGTTTTGCg          <  2:1078714/232‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          
GCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAG‑CGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGC‑GGGG‑ATAAACTCCGACGCCTGACGGTTACCGAGTGTG‑ATGGTGCCGG‑TTTTATCCAGTAGCAGAACGTCAACGT‑CACCTGCCGCTTCAACTG‑CACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTA‑CGCTGACTG‑CATTACCGCCCCACGCGGAAAACG‑GCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTCGTTCGGCGTTTTGCGTCGCTGTG  >  NC_000913/725948‑726408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A