Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F27 I0 R1	322	127.1	2757226	91.8%	2531133	223.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	758,596	G→C	14.6%	intergenic (+191/‑110)	sdhB → / → sucA	succinate dehydrogenase, FeS subunit/2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	758,596	0	G	C	14.6%	153.3 / 2.7	69	intergenic (+191/‑110)	sdhB/sucA	succinate dehydrogenase, FeS subunit/2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand): ref base G (37/19); new base C (7/4); total (46/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

TCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTG  >  NC_000913/758379‑758827

tCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCCC‑GAGCCACTCGGTAGACaaaa                                                                                                                                                                                                                      >  1:705661/1‑237 (MQ=255)

 gtttttttttgCAGCGGAATGCCGAACCCGTAGGCCTGAAATCGCGCGCAAGCG‑CCCAAGAGAGCACACGGTGCCGGACGGGGGGTGCACGCCTT‑TCCGGGCTAAAGAGTCATCCCGCGGAGGCCAGAAAAGCGCAGCGCATCAGGAGTAAAAAAAAAATGCAGAAATTCTTAAAAAACG‑CCCCCG‑GA‑CACAAAGACAGTTTTAAA‑AGTTTC‑CT‑TCGC‑GAGCCACTACGTAGACAagag                                                                                                                                                                                                                     <  2:361828/231‑1 (MQ=255)

    tgttgtTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCt                                                                                                                                                                                                                   >  1:264557/1‑238 (MQ=255)

    tgttgtTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑AACTAAGACAGTTTTTAA‑AAGTTC‑CT‑TCCC‑GATCCACTACGGAGACAAGACCt                                                                                                                                                                                                                   >  1:501953/1‑238 (MQ=255)

    tgttgtTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCCCATCAGGCGTAACAACGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GC‑AACCACAACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACCACGTAAACAAAACCTc                                                                                                                                                                                                                  >  2:843353/1‑239 (MQ=38)

                    tGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCCTCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGCCAAGACCTCGCAAGTGAACCCCgc                                                                                                                                                                                                   >  1:188212/1‑237 (MQ=255)

                           aCCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGACCGCCTTACCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GC‑AACCAAGACAGTTTTTAA‑AGGTTC‑CT‑TCAC‑GAGCCACCACGCAAACAAGAGACCGCAAGGGAACCCCGgcccgcca                                                                                                                                                                                            >  2:511106/1‑236 (MQ=38)

                               tAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAAAGCTCGCAAGTGAACCCCGGCCCGcac‑aac                                                                                                                                                                                        >  1:755138/1‑235 (MQ=255)

                               tAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCCC‑TGGCCCCTACGTAGACAAAAGCTCGCAAAGGAACCCCGGCACGCCC‑ATc                                                                                                                                                                                        >  1:1195097/1‑237 (MQ=39)

                                aGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAAAGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCCC‑GAGCCACCACGTAAAAAAAAACCCGCAAGAGAAACCCCGcacacac‑acc                                                                                                                                                                                        >  2:442090/1‑234 (MQ=38)

                                 gaatgtggaaaaagagccggCGTCCAATCAG‑GAAACCAGGGCCGGATGCGGGGTGAACGCCTTACCCGGCCTAAA‑AGTCATTCCCCGTAGCCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACG‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACt                                                                                                                                                                                     <  1:968265/219‑1 (MQ=255)

                                  gcaggATAAGCCCGGCAGGCGCCGCACAGG‑GCAACCGGGCCCGGATGCGGCGGAAACGCCTTACCCGGCTTAAA‑AGTCATTCCCCGTGGGCTTGATAAGCGCAGCGCATCAGGCTTAACAAAAAAAGGCAGAAAATTTTAAAAAACT‑CCCCTT‑GA‑CACTAAGAAATTTTTAAA‑AGTTCC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACt                                                                                                                                                                                     <  1:489694/233‑1 (MQ=255)

                                       aTAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑AAGCCACTACGTAGACAAAGGCTAGCAAGGGAAACCCggacgccccacaccgtgggggg                                                                                                                                                                               >  1:423446/1‑219 (MQ=255)

                                       aTAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCCC‑GAGCCACTACGTAGACAAAAGCTTGCAAGTGAACACCGGCACGCCC‑ATCCCTGTGctgg                                                                                                                                                                              >  1:1228994/1‑236 (MQ=255)

                                       aTAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AAGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCCCAATTGAACCCCGGCACGACC‑ACCACTGCGCGTg                                                                                                                                                                              >  1:1231103/1‑239 (MQ=255)

                                       aTAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AACTTC‑CT‑TCCC‑GAGCCACCCCGGCGCCCAGAGCTCGCAAACAAACCCCGGCACACAA‑ATCCCtgtggggg                                                                                                                                                                              >  2:77980/1‑235 (MQ=255)

                                                gcAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAAAGCTCGCAAGTGAACCCCGGCAC‑CACAACCACTGTGCGTGGGAGAATcc                                                                                                                                                                     >  2:1096411/1‑239 (MQ=255)

                                                 cAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAAAGCTCGCAAGTGAACCCCGGCACGcac‑acaccagagcggggaagctggc                                                                                                                                                                     >  2:489694/1‑217 (MQ=255)

                                                       tCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCCCTGTGCGTGGTAGTATCCACGGCGa                                                                                                                                                              >  1:701172/1‑239 (MQ=255)

                                                               g‑gCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCCCGCCC‑ATCACTGTGCGTGGTAGTATCCACGGCCAAGTAAGCa                                                                                                                                                      >  1:294404/1‑239 (MQ=255)

                                                                  cAACCGGGGCCGGATGGGGCGTAAACGCCTTAGCCGGCCTAAA‑AGTAATTCCCGGTAGCCTTAAAAGGCGCAGCGCATCAGGCGTAAAAAAAAAATGCAGAAATTCTTTAAAAACT‑GCCCCG‑GA‑CACAAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCAt                                                                                                                                                     <  1:1096411/238‑1 (MQ=255)

                                                                   cttgtaggCCGGATGCGGCGTGAACCCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑AACTAAGACAGTTTTTAA‑AGATTC‑CT‑TCCC‑GAGCCACTACGTAGACAAGAGCTCGCAAAGTAAACCCGGCCCGCCC‑CTCACTTCGCGTGTAAGTATCCCAGGCCAAGAAACCATaa                                                                                                                                                   >  1:533170/8‑239 (MQ=255)

                                                                         tgcgggtgggggcgTAACCCCCTTACCCGCCTAAAA‑AGTAATTCCCTGTAGGCTGGAAAGGCCCACCGCATCAGGCATAACAAAGAAATGCAGAAATTCTTAAAAACCT‑CCCCCT‑GA‑CACTAAGACAGTTTTAAA‑GGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAg                                                                                                                                              <  1:820590/228‑1 (MQ=255)

                                                                         tGCCGGATGGGGCGTGAACGCTTTACCCGGCCTACA‑ATTAATTACCCGTGGGTCTGATAAGCCCAGCCCATCAGGCGTAAAAAAGAAATGCAGGAAATCTTTAAAACCT‑GCCCCT‑GA‑CCCTAAGACATTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAg                                                                                                                                              <  1:442090/238‑1 (MQ=255)

                                                                         tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTTCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGa                                                                                                                                             >  1:267202/1‑239 (MQ=255)

                                                                          gCCGGTGGCGGCTTAACCGCCTTACCCGGCCTACA‑ATTCATTCCCCGTAGGCCTAAAAAGCCCAGCGCATCAGGCGTAACAAAGAAATGCAGAAAATCTTAAAAACCT‑CCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGTTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGa                                                                                                                                             <  1:384885/238‑1 (MQ=255)

                                                                          ccggggtgggggggaaaCGCCTTATCCGGCCTAAA‑AGTCATTCCCGGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAAAAAAGAAATGAAGAAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACATTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAg                                                                                                                                              <  1:1026292/222‑1 (MQ=255)

                                                                                        gAACGCTTAACCCGGACTAAA‑AGTCAATCCCGGTAGGCCTGAAAAGACCAGCCCACCAGGCATAAAAAAAAAATGAAGGAATTTTTTAAAACCG‑GCCCCT‑GA‑CACAAAGACATTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCa                                                                                                                               <  1:671546/238‑1 (MQ=255)

                                                                                        gAACGCCTTCACCGCGCTACA‑AGTCATTACCGGTGGGCCTGAAAAGCGCAGCGCATGGGGCGTAAAAAAGAAATGCAGAAAATTTTAAAAAACT‑CCCCCA‑GA‑CAATAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCa                                                                                                                               <  1:1285846/238‑1 (MQ=255)

                                                                                            cttttctgcGGCCTAAA‑AGTGATTCCCCTGGGGCCGAAAAAGCGCACCCAAGCAGTAGTAAAAAAAAAGTGCAGGAACTTTTAAAAACCT‑GCCCCT‑GA‑CACTAAGGCATTTTTTAA‑GGGTTC‑CG‑TCCG‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATg                                                                                                                          <  2:501953/231‑1 (MQ=255)

                                                                                                       ccTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCCC‑ATCACTGTGCGTGGTAGTATCAACGGCGAAGTAAGAATAAACAAGATGCTTAAGGGAGCAAAATGCAGaaaaaggc                                                                                                               >  2:587820/1‑233 (MQ=255)

                                                                                                                  aTTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATACACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATAACGATGCAGAACAGGGCTTTGAAAg                                                                                                       >  1:331085/1‑237 (MQ=255)

                                                                                                                    tACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAAATGCTTAAGGGATCACGAGGCCAAACAGCGCTTTGAAAACCTg                                                                                                   >  2:146883/1‑239 (MQ=255)

                                                                                                                      cccGTAGGCCTGATAAGCGCAGCGCATAAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑AT‑TCGC‑GCGCCACTACGTAGACAAGAGCGCGCAAGTGAACACCGGCACGCAC‑ATCACAGTGCGTGGTAGTATACACGGCGAAGTAAGCATAAAAGAGAAACTTAAGGGAGCAATATACAGAAAAGACCTTTaaaaacgtgtg                                                                                                 >  2:765613/1‑232 (MQ=255)

                                                                                                                           gggCTTGAAAAGCGCAGCGCATGAGGCATAAAAAAAAAAGGCAGAAAATCTTTAAAAACT‑GCCCGC‑GA‑CACAAAGCCGGTTTTTAA‑AGTTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGa                                                                                             <  1:843353/237‑1 (MQ=255)

                                                                                                                           aGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAAAAGCGCTTTGAAAGCCTGGTTgg                                                                                              >  1:638896/1‑237 (MQ=255)

                                                                                                                                       cgcagcgcaTCACGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCAGGTACGCAC‑CTCACTGTGCGTGGTAGAATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGAGTCAGAACAGCGATTTGCAAGCCAGGGTGGA‑CCCTTTTTACct                                                                                >  2:242758/1‑238 (MQ=255)

                                                                                                                                         cagcgcaTCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGCCAAGAGCTCGCAAGTGAACCCCGGCACGAAC‑ATCACTGTGCGTGGTAGTATCCCAGGCGAAGTAAGCATAACAAAGACGCTTAAGGGATCACGATTCAGAAAAGCCATTTGAAAGCCTGCTTGGA‑CCCTTCTTACctc                                                                               >  2:889236/1‑237 (MQ=255)

                                                                                                                                            cgcaTCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑TTCTTCTTACCTCTCTg                                                                           >  1:1222631/1‑238 (MQ=255)

                                                                                                                                            cgcaTCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTg                                                                           >  1:990563/1‑238 (MQ=255)

                                                                                                                                            cgcaTCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCAACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGAAGCAGAACAGCGATTTGAAAGCCTGGTTGGA‑ACCTTCTTACATCTCTgg                                                                          >  2:484839/1‑239 (MQ=255)

                                                                                                                                            cgcaTCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGGGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAAAAGCGCCTTTAAAGCCTGGTTGGA‑CTCTTCTTAccccccggg                                                                          >  2:1236632/1‑232 (MQ=255)

                                                                                                                                                 cAGGCGTAACAAAGAAATGCAGGAAATCTTTACAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGACAACAAGACCTCGCAATTGAACCCCGGCACGCAG‑ATCACTGTGCGTGGAAGGATCCACGGACAAGTAAGCATAAAAAAGACGCTTAGGGGATCACGCTGCCGAACAGCGCTTTGAAAACCTGGTTGGACCTCTT‑TTACctctcttggcgaa                                                                     >  2:1006298/1‑232 (MQ=255)

                                                                                                                                                 cAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCAC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGACGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTAcccccctatgcg                                                                       >  1:435204/1‑227 (MQ=255)

                                                                                                                                                      gTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCag                                                                >  1:1035497/1‑239 (MQ=255)

                                                                                                                                                        aaaaaaGAAATGCAGAAAATCTTTAAAACCT‑GCCCCT‑GA‑CACTAAGACAGTTTTAAA‑AGGTCC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCagag                                                              <  1:146883/236‑1 (MQ=255)

                                                                                                                                                         aaaaaaaaaagaaaaaaaatttaaaaaaCC‑GCCCCT‑GACCACAAGGCTTGTTTAAAA‑AGTTTC‑CT‑CGGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCagag                                                              <  2:638896/216‑1 (MQ=255)

                                                                                                                                                         aCAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTGTTCTTACCTCCCTGGCGCAAACCAGAGc                                                             >  1:490901/1‑239 (MQ=255)

                                                                                                                                                         aCAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGCTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCaga                                                               >  1:701116/1‑237 (MQ=255)

                                                                                                                                                                  tGCAAGAATTTTTAAAAACCG‑GCCCCT‑AA‑CACAAAGA‑AGTTTTGAACAGTTTC‑CT‑CACT‑GAGCCACTACGTAGACAAGATGTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGaa                                                    <  1:649009/239‑1 (MQ=255)

                                                                                                                                                                   tgagaaaaaCTTTAAAACCTCGCCCCG‑GA‑CAAGAAGAACGTTTTAAA‑GGGTCC‑CT‑GCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGaa                                                    <  1:1084169/234‑1 (MQ=255)

                                                                                                                                                                       gAAATCTTTAAAAACT‑GACCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGCCAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAACAGATGCTTAAGGGATCACGATGCCGAACAGCGCTTTCAAAGCCAGGTTGGC‑CTCTCCTTCCCCCTCTGGCCCACAccgaggttggaaagaaaaac                                                >  2:1138611/1‑220 (MQ=255)

                                                                                                                                                                              atAAAACCT‑GCCCCT‑GA‑AACTAGGCATGTTTTTAA‑AGTTCC‑CT‑TCGG‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGaa                                        <  1:119615/238‑1 (MQ=255)

                                                                                                                                                                               aaaaaaCT‑CCCCCTGGA‑CACAAAG‑CTGGTTTAAA‑AAGGTC‑CT‑TCCCGGGGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGaa                                        <  2:267202/238‑1 (MQ=255)

                                                                                                                                                                                        gCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGCAGTATCCACGGCGACGTAAGCATCAAAAAGTTGCTTAAGGGATCACGCTGCCGAACAGCCCTTTCAACGCCTGGTTGGA‑CTCTCCTTACCTCCCCGCCGCAACACACAGCCGGATAGAACAGcccttcaccaaactctta                               >  2:96362/1‑225 (MQ=255)

                                                                                                                                                                                                     tAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCtt                     >  1:1375295/1‑237 (MQ=255)

                                                                                                                                                                                                            tttttaAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAAc                             <  1:950060/222‑1 (MQ=255)

                                                                                                                                                                                                            gTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAAc                             >  2:950060/1‑223 (MQ=255)

                                                                                                                                                                                                            cattttAA‑AGGGTCTCT‑GCCG‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGt              <  1:729032/237‑1 (MQ=255)

                                                                                                                                                                                                             tttttAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAAAAGCTCTATGAAGACTTCTTAAACAAACCTGACTCGGTTg            >  2:715780/1‑239 (MQ=255)

                                                                                                                                                                                                             tttttAA‑AGGTTC‑CT‑TCGC‑GAGCCACAACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTg            >  1:247729/1‑239 (MQ=255)

                                                                                                                                                                                                             gtttata‑aGGTTC‑CTGCCGG‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGGGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGtt             <  1:587820/233‑1 (MQ=255)

                                                                                                                                                                                                               tttAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAACGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGAACCTGAATCGGGTGAc          >  2:76634/1‑239 (MQ=255)

                                                                                                                                                                                                                        ttC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATa                                                                                                                                                    >  1:238215/1‑94 (MQ=255)

                                                                                                                                                                                                                        ttC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATa                                                                                                                                                    <  2:238215/94‑1 (MQ=255)

                                                                                                                                                                                                                        ttC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAACCCAGAGCTGGAAAGAAACGCCCTATGAAGCCTTCTAAACCGATccaaaacaggttaacgcaaaccg  >  2:669754/1‑218 (MQ=255)

                                                                                                                                                                                                                        ttC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTg  >  1:1308989/1‑239 (MQ=255)

                                                                                                                                                                                                                            cT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAAc                             <  1:155472/209‑1 (MQ=255)

                                                                                                                                                                                                                            cT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAAACTTCTTAAc                             >  2:155472/1‑209 (MQ=255)

TCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GA‑CACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTG  >  NC_000913/758379‑758827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A