Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 817,807 A→C 12.0% D255A (GAC→GCC)  moaA → molybdopterin biosynthesis protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913817,8070AC12.0% 242.8 / 3.3 107D255A (GAC→GCC) moaAmolybdopterin biosynthesis protein A
Reads supporting (aligned to +/- strand):  ref base A (75/14);  new base C (3/13);  total (78/29)
Fisher's exact test for biased strand distribution p-value = 5.07e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TTCTGAACTGGATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGG  >  NC_000913/817573‑818045
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               
ttCTGAACTGGATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGCGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGCTCCACCAATTACGTCAACGCAGCGACGGTCCCGCCCAAGTCTTTTGCCCTCCAGATTCCCCCGGAGGGATTGGCCT‑TATCCTCCCGTCTGAACAAGAAtta                                                                                                                                                                                                                                             >  2:952476/1‑238 (MQ=255)
ttCTGAACTGGATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TCTCATTCCGTATGAAAAAAACTTc                                                                                                                                                                                                                                             >  2:233170/1‑239 (MQ=255)
ttCTGAACTGGATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCCCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACt                                                                                                                                                                                                                                               >  1:295094/1‑237 (MQ=255)
    gacttGGATCCGACATTGCCCTACCCAGGTTGGTTTCGCGGAACTGATGGAAACGGGGGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCGACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCGTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTgcg                                                                                                                                                                                                                                         <  2:1036185/235‑1 (MQ=255)
        tGGATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCAc                                                                                                                                                                                                                                    <  1:1215349/239‑1 (MQ=255)
        tGGATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAAAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑cc                                                                                                                                                                                                                                      >  1:117807/1‑237 (MQ=255)
          gATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAACCGGGCGAGTGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCCCCAATTACGTCAACGCAGCGCCGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACCCCGGAGAGATTGGCCT‑TATCATCCCGTTTGAAAAACACTTCTCCG‑CCACtt                                                                                                                                                                                                                                  >  2:1049754/1‑239 (MQ=255)
          gATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGGGAGATTGGCCT‑TATCATGCCGCATGCAAAAGACTTTTCCG‑CCCCtt                                                                                                                                                                                                                                  >  2:346761/1‑239 (MQ=255)
          gATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCTGATGAAAAAGACTTCTGCG‑CCACt                                                                                                                                                                                                                                   >  1:427465/1‑238 (MQ=255)
          gATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCCCGCAAGTCTTTTGCCAACCAGATTTCGACGGACAGATTGGCCT‑TATCATGCCGTTTGAAAAAAACTTCAGCG‑CCACtt                                                                                                                                                                                                                                  >  2:1060539/1‑239 (MQ=255)
                 cATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACCAAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGCTTCCGCCGGGAAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTGTCAAcc                                                                                                                                                                                                                            >  2:1358221/1‑238 (MQ=255)
                      cccTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGCTTACACCGGAGAGATTGGCCT‑TATCATCCCGTTTGAACAAGACTTCTGCG‑CCCCTTGAAACCGCCTgc                                                                                                                                                                                                                      >  2:571103/1‑239 (MQ=255)
                      cccTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGCGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTgc                                                                                                                                                                                                                      >  1:825152/1‑239 (MQ=255)
                      cccTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTCGG‑CCCCTTGCAACCGCCTgc                                                                                                                                                                                                                      >  1:1099212/1‑239 (MQ=255)
                           tCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGtt                                                                                                                                                                                                                 >  1:1032876/1‑239 (MQ=255)
                           tCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGtt                                                                                                                                                                                                                 >  1:222014/1‑239 (MQ=255)
                           tCCAGCTGCGATTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGCGAGATTGGCCT‑TATCATGCCGGAGGAAAAAGACTTCTTCG‑CCACTTGCAACCGCCTCCGCGt                                                                                                                                                                                                                  >  1:54111/1‑238 (MQ=255)
                                cTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCACTTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGCTTGGCCT‑TATCATGCCGACTGAAAAAGACTTCTTCG‑CCACTTTCAACCGCCTGCCCGTTtcctc                                                                                                                                                                                                            >  2:799547/1‑239 (MQ=255)
                                cTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTtcct                                                                                                                                                                                                             >  1:975907/1‑238 (MQ=255)
                                   cGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGGG‑CCACTTGAAACCGCCTGCGCGTTTCCCCCAt                                                                                                                                                                                                         >  2:1105545/1‑239 (MQ=255)
                                   cGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCAt                                                                                                                                                                                                         >  1:1064947/1‑239 (MQ=255)
                                     tttCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGGG‑CCCCTTGAAACCTCATGCGCGCTTCGTCCATTg                                                                                                                                                                                                       >  1:904173/1‑239 (MQ=255)
                                     tttCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGAGACGGTCCCGCGCAGGTCTTTTGCCATCCAGATTACGCCGGGGAGAGTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTg                                                                                                                                                                                                       >  2:1283201/1‑239 (MQ=255)
                                     tttCATCGAACTGATGGAAACGGGCGAGGCCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGTAGAGATTGGCC‑‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCGCCATTgt                                                                                                                                                                                                      >  1:578542/1‑238 (MQ=255)
                                         aTCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCCGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTaa                                                                                                                                                                                                   >  1:679735/1‑239 (MQ=255)
                                                      gggagttgtgtaaaagagacagctTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGGCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGATTTCTGCG‑CCCCTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTg                                                                                                                                                                                       <  1:1218218/216‑1 (MQ=255)
                                                        aCGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCTCGCGCGTTTCCTCCATTGGCAAAATCCACCCCTg                                                                                                                                                                                       >  2:116515/1‑236 (MQ=255)
                                                          gggCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGt                                                                                                                                                                                  >  1:249023/1‑239 (MQ=255)
                                                            gCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGGTTGGCCT‑TATAATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCCCCTGCCCGTTTCCTCCATTGGTAAACTCCATACCTGCCTGttt                                                                                                                                                                                >  2:275014/1‑239 (MQ=255)
                                                                 ggCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCAATGGTAAACTCCACCTCTGCCGGTTTTGTGa                                                                                                                                                                           >  2:197757/1‑239 (MQ=255)
                                                                 ggCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAAAGCAGCGACGGTCCCGCGCAAGGCTTTTGCCATCCAGCTCAACCCGACGAGAGTGGCCT‑TATCATGCCGAATGGAAAGACCTTTGGCG‑CCAATTGAAACCCCATCGGCGTTTCCCCCATTGGTAAAATCACTCTCTCCCCGTTTGgaga                                                                                                                                                                           >  2:1014774/1‑239 (MQ=255)
                                                                    aGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTCTGAAAAAGCCTTCTCCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACACCACCCCTGCCTGTTTGGGGAAgg                                                                                                                                                                        >  2:692279/1‑239 (MQ=255)
                                                                      cGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGCCGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAAACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGCTAAAACCCACCTCTCCCTTTTTGGGCAAggcg                                                                                                                                                                      >  2:853446/1‑239 (MQ=255)
                                                                      cGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTCCC‑CCACCTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCCTCTCTGCCGCTTTGGTGCAggcg                                                                                                                                                                      >  2:7251/1‑239 (MQ=255)
                                                                                  tAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGCCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCCTTTTAAAAAGACTTCTGCG‑CCCCTGGCAACAGCCTGCCCGTTTCCTCCATTCTTAAACTCCACCCCTCCCTGTTCGGTTAAGCGCGCGGTAAccctc                                                                                                                                                          >  2:193127/1‑236 (MQ=255)
                                                                                       aTCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGGATGAAAAAGACTTCTTCG‑CCTCTTGCAACCGCCTGCGCGTTTCCTCCATTGGAAAACTCCATCTCTGCCTTGTTGGTGAAGGCGGCGTTAACCTGCGCGa                                                                                                                                                      >  1:818421/1‑238 (MQ=255)
                                                                                       aTCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCGGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGCCTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGAt                                                                                                                                                     <  1:233170/239‑1 (MQ=255)
                                                                                         cacaTCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGa                                                                                                                                                      >  1:917478/1‑236 (MQ=255)
                                                                                         cacaTCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGATTGGTGAAGGCGGCGTTAACCTGCGCGa                                                                                                                                                      >  1:1171436/1‑236 (MQ=255)
                                                                                             cctctGGTAGGTTTCTGCGGGCCGAGCTCCGGCCTCGGGGCTGGACCCACCAATTCCGTCACCGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCTCTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATctgctg                                                                                                                                               <  2:918256/238‑1 (MQ=255)
                                                                                                ctGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCACCCGCCTGCGCGCTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAGGGCGGCGTTAACCTGCGCGATCTCCTGGaa                                                                                                                                            >  1:121964/1‑239 (MQ=255)
                                                                                                ctGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTTCGCGTTTCCTCCCTTGGTAAACTCCCTCTCTGCCTGTTTGGTGGAGGCGGCGTTAACCTGGACGATCTTCTGGag                                                                                                                                            >  2:1050182/1‑238 (MQ=255)
                                                                                                ctGGTCAGGTACTGCGAGACAAGCTACTGCGTCGCGGCTGGATCCACCAATTACGCCAACGCAGCTACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACATCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGGGAAGGCGGCGTTAACCTCCGCGACCTACTGGaa                                                                                                                                            >  2:908443/1‑239 (MQ=255)
                                                                                                   cgggttttCTGCGTTGCGGGCTTCTGCGTCGGGGGTGGCTCCCCCAATTCCGTCAACGGCGCGGGGGTCCCGCGCAAGTTTTTTGCCATCCAGATTACGCGGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCC‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGa                                                                                                                                          <  2:124531/232‑1 (MQ=255)
                                                                                                        gTTCTGCGTGACGAGCTACTGCGGCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGACACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGCGAAGGCGGCGTTAACCGGCCCGACCAGCAGGaaaagaaaa                                                                                                                                     >  2:183325/1‑231 (MQ=255)
                                                                                                              cGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCCTTGGTAAACTCCATCTCTCCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGaaaaggaaaaccaaaa                                                                                                                              >  2:706256/1‑225 (MQ=255)
                                                                                                              cGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCa                                                                                                                              >  1:70160/1‑239 (MQ=255)
                                                                                                                  aCGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAg                                                                                                                                >  1:124531/1‑233 (MQ=255)
                                                                                                                  aCGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAg                                                                                                                          >  1:546906/1‑239 (MQ=255)
                                                                                                                  aCGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTCCGCGATCTGCTGGAAGAGGATACCCAGCAACAg                                                                                                                          >  1:201725/1‑239 (MQ=255)
                                                                                                                  aCGAGCTACGGCCTGGCGGCTGGAACCACAAATTCCGTCAACGCCGCGACGGTCCCGCGAAAGTCTTTTGCCACCCAGTTTCCGCCGGAAGTATTGGCCT‑TACCAGGCCGTTTGAAAAAGACTTCGGCC‑CCCCTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACa                                                                                                                           <  1:772056/238‑1 (MQ=255)
                                                                                                                      gCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGACGACGATACCCAGCAACAGgcgc                                                                                                                      >  1:551412/1‑239 (MQ=255)
                                                                                                                               tCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTTCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTACCCTGCGCGATCTGCTGGAAACCGATACCCGGCAACAGGCGCTGGAAgcgg                                                                                                             >  1:59374/1‑238 (MQ=255)
                                                                                                                                cgcgGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGCCTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGc                                                                                                                                                                                      <  1:1208473/165‑1 (MQ=255)
                                                                                                                                cgcgGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGc                                                                                                                                                                                      >  2:1208473/1‑165 (MQ=255)
                                                                                                                                    gCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTTCGCGATCTGCTGGAAGACGATACCCAGCAAAAAGGCCGGGAAGCGCGTaat                                                                                                        >  2:810405/1‑237 (MQ=255)
                                                                                                                                                  ttgccgcaaccgggcggcgGCCCCGCACAAGTCTTTTGCCATCCAGGTTACCGCGGGGAG‑TGGGCCTCGATCAGGCCGTATAAAAAAGCCGTCGGCG‑CCACGTGCAAGCGCCTGCGCGTTTCCTCCAATGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCg                                                                                          <  2:696945/226‑1 (MQ=255)
                                                                                                                                                  ttACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCg                                                                                          >  1:574068/1‑239 (MQ=255)
                                                                                                                                                      gTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTCCTGGAAGACGATTCCCGGCACCAGGCGCTGGAACCGCGCATTTAACCGGCGCGACGGgag                                                                                      >  2:1262216/1‑239 (MQ=255)
                                                                                                                                                       tCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTTACAGGCGCTGCGGggga                                                                                     >  1:696945/1‑239 (MQ=255)
                                                                                                                                                       tCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGTTGCGGgagc                                                                                     >  1:1339624/1‑238 (MQ=255)
                                                                                                                                                       tCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCCCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCGGGAAGCGCGTATTTCAGCGGCGCTGGGGggga                                                                                     >  2:1157220/1‑239 (MQ=255)
                                                                                                                                                       cgctcgcgggtatggTCCCGCGCTAGTCTTTTTCCATCCAGTTTAGCGCGGAGTGTTGGGCTT‑TGTCTCGCCTTTAGAAAAAGACTTCGGCC‑CCTCTTACAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGgaga                                                                                     <  2:608785/226‑1 (MQ=255)
                                                                                                                                                         aaCGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGTAACCCAGCAACACGCCCTGGAACCGCGTCTTTCAGCGGTGCTCCgggtgcag                                                                                   >  1:53396/1‑234 (MQ=255)
                                                                                                                                                                 gccGGGCCCGGGCAAGGCTTTTGCCAGCCAGTTTCCGCCGGAGAGATTGGCTT‑TATCAGGCCGTATGAAAAAGCCTTCTGCG‑CCTCTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGa                                                                            <  1:1273810/236‑1 (MQ=255)
                                                                                                                                                                         ggcggAAGTCTTTTCCAATCAAGTTTACGCGGGAGAGATTGGCTT‑TATCATGCCGTATGAAAAAGACTTCGTCG‑CACCTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTcc                                                                   <  1:275014/238‑1 (MQ=255)
                                                                                                                                                                         cgctCAAGTCTTTTCCCATCCAGATTACGCCGGAGAGATTGGCCT‑TACAATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTcc                                                                   <  1:197757/239‑1 (MQ=255)
                                                                                                                                                                              aaGTCTTTTGCCATCCAGATTCCCCCGGAGAGATTGCCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCCCTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCAt                                                              <  1:1105545/239‑1 (MQ=255)
                                                                                                                                                                                      tccctccacagttcggCCGGAAGGTTTGGCCT‑TCTCGTGCCTTAGGAAAATGCCTCCGCCC‑CCGCTTGCAACCGCCTGCGGGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAaacaa                                                      <  2:232613/224‑1 (MQ=255)
                                                                                                                                                                                         cATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGCCGTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAacac                                                   <  1:116515/239‑1 (MQ=255)
                                                                                                                                                                                           tCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATa                                                                                                                                     >  1:1024842/1‑155 (MQ=255)
                                                                                                                                                                                              ggATTACGCCGGAGAGATTGGCCT‑TATCTTGCGGTAGGAAAAAGCCTTCTGCC‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACGCCGGTa                                              <  1:952476/238‑1 (MQ=255)
                                                                                                                                                                                                     gCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGCAGCGCGGATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCGGCATCAAAACAACCCCGGGATTACgaa                                       >  2:1347813/1‑237 (MQ=255)
                                                                                                                                                                                                     gCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCa                                                                                                     >  1:254595/1‑177 (MQ=255)
                                                                                                                                                                                                     gCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCCGCCTGTTTGGGGAAGGCGGCGTTAACCTGCCCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAACCGCGTATTTCCACCGCGCTGCGGGAGAAGAAACAAAACCATTTCCTGAATCAACAAAACACAGGGATAAcaca                                       >  2:754636/1‑239 (MQ=255)
                                                                                                                                                                                                     gCCGGAGAGATTGGCCT‑TATCAGGCCGTTTGAAAAAGACTTCGGCG‑CCAATGGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCa                                       <  1:1262216/239‑1 (MQ=255)
                                                                                                                                                                                                     cggggaagagttGGCCT‑TACCATGCCGTATAAAAAAGCCTTCGGCC‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCa                                       <  1:7251/229‑1 (MQ=255)
                                                                                                                                                                                                     cccGGAGATTTGGTCCT‑TATCCGGCTGTA‑GAAAAAAATTTGCGCGCCCTCTTTCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCACTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCa                                       <  2:1032876/238‑1 (MQ=255)
                                                                                                                                                                                                       gggggataTTTGCCT‑TTTCAGTCCGTATGAAAAAGCTTTCTGCC‑CCCCTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCaaa                                     <  1:1187990/232‑1 (MQ=255)
                                                                                                                                                                                                       gggaaagttcgcccT‑TATCATGCCGTATGAAAAAGTCTTTCGCG‑CCACTGGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCaaa                                     <  1:1136924/227‑1 (MQ=255)
                                                                                                                                                                                                             ggtgggCCT‑TTTCAGCCCTTATAAAAAAGCCTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTa                                <  1:692279/234‑1 (MQ=255)
                                                                                                                                                                                                             atttGCCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTa                                <  1:1358221/236‑1 (MQ=255)
                                                                                                                                                                                                              aTTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCTGCGTTAACCTGTGCGATCTGCTGGAAGCCGATACCCAGCAACAGGCGCTGCAAGCGCGTATTTCAGCGGCGCTGCGGAGGGAGAAACAGACCCATTTCATGCATCAAAACAAAACCGGAATAAGACAAAAATTATc                              >  2:1136924/1‑239 (MQ=255)
                                                                                                                                                                                                              aTTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAAAAACAGACCCATTTCCTGCATCAAACCAACACCGGTATTACGCAAAACTCATc                              >  1:988390/1‑239 (MQ=255)
                                                                                                                                                                                                              aTTGGCCC‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGCGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATc                              >  1:352101/1‑239 (MQ=255)
                                                                                                                                                                                                                       tacagtGCCTTAGGAAAAAGCCTTCTGCG‑CCCGTTGCAACCGCCTGCGCGTTTCCGCCATTGGTAAACTCCAGCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTg                      <  1:1049754/234‑1 (MQ=255)
                                                                                                                                                                                                                        aTCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCTGCGCTGCGGGAGAAGAAACAGACACATTTCCTGCATCAAACAAACAACGGTATTACGCAAAACTTATAGTACATTg                      >  1:74156/1‑238 (MQ=255)
                                                                                                                                                                                                                        aTCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGggaaga                                                                                  >  1:239315/1‑178 (MQ=255)
                                                                                                                                                                                                                         tCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGCGAAGAAACAGACCCATTTCCTGCATCAAAAAAAAACCCGGATTACGCAAAACTTAACGAACATTggc                    >  2:141090/1‑239 (MQ=255)
                                                                                                                                                                                                                              ccGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTa               >  1:352250/1‑239 (MQ=255)
                                                                                                                                                                                                                              ccGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTa               >  1:999381/1‑239 (MQ=255)
                                                                                                                                                                                                                              ccGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTa               >  1:950708/1‑239 (MQ=255)
                                                                                                                                                                                                                               cGTAGGAAAAAGCCTTCGGCG‑CCCCTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTaa              <  1:1050182/239‑1 (MQ=255)
                                                                                                                                                                                                                                 tATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGGGCTGCGGGAGAAGAAACAGACCCCTTTCCTGCATCAAAACAACACCGGTATTACGCAAAACATACCGTAAATTGGCGGaaaaaa            >  2:405312/1‑239 (MQ=255)
                                                                                                                                                                                                                                  aTGAAAAAGATTTCTGGC‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAAc           <  1:706256/239‑1 (MQ=255)
                                                                                                                                                                                                                                   tGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGCCGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGGGAAGACACAGACCCATTTCCTGCATCAAAACAACACCGGTATTCAGCAAAACTTCTCGTACATTGGCGGCTAAAAc           >  1:366978/1‑238 (MQ=255)
                                                                                                                                                                                                                                   tGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACCGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCACTTTCCTGCATCCAAACAACACCGGGATTAAGCAAAACTTATCGTACATTGGCGGGTAAAACg          >  2:372222/1‑239 (MQ=255)
                                                                                                                                                                                                                                   tGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACACCACCGGTATTACGCAAAACTTATCGTACATTGCCGGCTAAAAc           >  1:517026/1‑238 (MQ=255)
                                                                                                                                                                                                                                           aCTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCTTACATTGGCGGCTAAAACGCCAAAAgg  >  1:494186/1‑239 (MQ=255)
                                                                                                                                                                                                                                           aCTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAgg  >  1:155969/1‑239 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               
TTCTGAACTGGATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCT‑TATCATGCCGTATGAAAAAGACTTCTGCG‑CCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATTTCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATTACGCAAAACTTATCGTACATTGGCGGCTAAAACGTCAAAAGG  >  NC_000913/817573‑818045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A