Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,543,530 A→C 14.1% S177R (AGT→AGG)  narU ← nitrate/nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,543,5300AC14.1% 230.2 / 3.5 96S177R (AGT→AGG) narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base A (54/26);  new base C (4/12);  total (58/38)
Fisher's exact test for biased strand distribution p-value = 3.84e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGG‑CGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAATAATCAGGATTGCAGTACTAAAAACCGTCCATCGGCGTCC  >  NC_000913/1543294‑1543766
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              
atccAGAGGAGCAGGGTTTGTAAGCCAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGG‑CGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCAttacact                                                                                                                                                                                                                                              <  1:852231/237‑1 (MQ=255)
        ttGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGG‑CGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCt                                                                                                                                                                                                                                      <  1:476160/237‑1 (MQ=255)
        aTGGAGGCGTTGAAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGG‑CGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCt                                                                                                                                                                                                                                      <  1:409058/238‑1 (MQ=255)
          gcgggggTTGTAAAACAGGGGGGTGGTCGGAAATTGGGGGGCGTGAACTGG‑GTATATCATTCACCCCTGACCAGGCGGGCATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTaa                                                                                                                                                                                                                                    <  2:893889/232‑1 (MQ=255)
                     aaGACGGGGGTCTTGTCGGAAATTGAGGGTGGGGAACGGGCCAATAT‑ATTCCTCCCTGCCCGTGCGGCGATCGTGGCAATCCCCAGTGGCGGTACCCAAATCCATGCGGCATTCGCCGGCGACAACACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATcc                                                                                                                                                                                                                        <  2:402690/239‑1 (MQ=255)
                            ggggttgtctgggattgggggCGGGTGAACGGG‑CTATACCATTCACCCCGGCCCAGGGGGCGATGGTGGAAATCGCCAGTAGCGGGCCCAAAATCCAGGGGGCATTCGCCAGCGACATCCCGGAACCGGCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATGC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCAAATCCGCCAtt                                                                                                                                                                                                                  <  2:112630/219‑1 (MQ=255)
                            ggAGCTGGTCGGCAATTGAGGCGCGTGAACTGG‑CGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGATTCCTAATCCGCCATTa                                                                                                                                                                                                                 >  1:587210/1‑239 (MQ=255)
                            ggAGCTGGTCGGCAATTGAGGCGCGTGAACTGG‑CGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGAACGCC‑ATTGAAGCCGGGAAAGGCAAATAC‑AGG‑AACAAAAATGA‑CCAGCGGGGCAACCAGCTCCCTTCCACTTACA‑CCTAAGTTTCCTAATCCGCCCTTa                                                                                                                                                                                                                 >  1:276348/1‑239 (MQ=255)
                                  ggTCGGCAATTGAGGCGCGTGAACTGG‑CGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCCAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAAAAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCCACTGCATTACCCTTACA‑CCTAAGTTTCCTAATCCGCCATTAAAccaa                                                                                                                                                                                                           >  2:879450/1‑237 (MQ=255)
                                  ggTCGGCAATTGAGGCGCGTGAACTGG‑CGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCa                                                                                                                                                                                                           >  1:22344/1‑239 (MQ=255)
                                                   cgTGAACTGG‑CGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCAttacacttaca‑c                                                                                                                                                                                                                                        >  1:126956/1‑193 (MQ=255)
                                                   cgTGAACTGG‑CGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGttt                                                                                                                                                                                         >  1:102591/1‑239 (MQ=255)
                                                         cTGG‑CGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCA‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCttt                                                                                                                                                                                   >  1:402613/1‑239 (MQ=255)
                                                                       tcatCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGCCGCCGAGAAAGGCAAATAC‑AGG‑GACAAAAATGA‑CCAGCGGGGCAACCAGCTGCATTACACTTACC‑CCTAAGTTTCCTAATCCCCCATTAATCCCAAAACACCCCCC‑TTTTTTTGCTTTTGGAAAGAGGaaa                                                                                                                                                                      >  2:1303036/1‑239 (MQ=255)
                                                                       tcatCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAgaagaa                                                                                                                                                                       >  1:1067991/1‑238 (MQ=255)
                                                                       tcatCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGaaa                                                                                                                                                                      >  1:983329/1‑239 (MQ=255)
                                                                       tcatCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATACCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAgacgaa                                                                                                                                                                       >  1:937317/1‑238 (MQ=255)
                                                                           cccTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAAAGCGCCCCC‑TTGTTTGGCTTTTGGaaaaaaaaaaataa                                                                                                                                                                  >  2:1171837/1‑235 (MQ=255)
                                                                           cccTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACAGTCGGCCTGCGGAACGCC‑ATTGACCCCGAGAAAGGCAAATAA‑AGG‑AACAAAAACGT‑CCACCGGTGTAACCAGCTGCATTACACTAAAA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAAAGGGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAAATGa                                                                                                                                                                  >  2:816156/1‑239 (MQ=255)
                                                                             cTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGata                                                                                                                                                                >  1:492370/1‑239 (MQ=255)
                                                                              ttaCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGCCTTTCGCCAGCGTCATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTTACGCAGAGAAAGGGAAACCC‑AGG‑GACAAAAATGA‑CCAGCGGTGCAACCAACGGCATTAACCTTCAA‑CCTACTTTTCTTCAACCCCCTTTAGTCCCAAGAGCGCGCCC‑TTGTTTGGATTTTGGAAAAAAAAAAccaataa                                                                                                                                                               >  2:1006775/3‑233 (MQ=255)
                                                                               gACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCGGCATTACACTTACA‑CCTAAGTTTCCTAATCCCCCATTAACCCCAAAATCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAAAAACTGaacat                                                                                                                                                              >  2:136985/1‑235 (MQ=255)
                                                                                aCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACAGatatg                                                                                                                                                              >  2:1121993/1‑237 (MQ=255)
                                                                                aCCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCAATGCGGCATTCGCCAGCGACATCCCCGAACCGTCGGCCTGCGGTAAGCC‑ATTGACGCCGAGAAACGCAAATAC‑AGG‑AACAAAAATGACCCAGC‑GCGCAACCAGGTGCATTACACTTACC‑ACTAAGTTTCCCAACCCGCCATTAATAACAAAACCGATCCC‑GTGTTTGGCCTTTTGAAAGAAAAAACAGATAtt                                                                                                                                                              >  2:1186476/1‑238 (MQ=255)
                                                                                aCCACGCGGCGATCGTGGCAATCACCAGTATCGGTACCCATATCCATGCGGCATTCGCCACCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGCCGCCGAGAAAGGCAAATCC‑AGG‑CACAAAAATGA‑CCAGCGGCGCAACTACTTCCCTTAACCTTAAC‑CCCTAGCTTCCTAATCCGCCATTAA‑CCCAAAAACCATCCCGTAGTTTGCATTTTGGaaaaaaaaaattatctat                                                                                                                                                              >  2:644400/1‑230 (MQ=255)
                                                                                     tcggcgATCGTGGCAATCGCAAGTAGCGGTACCCAGATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTACTCCCCAGAGCGCTACC‑TTGTTTGGCTTTTGTAAAGAATAAACTGATATTGCCCAt                                                                                                                                                        >  2:197399/2‑239 (MQ=255)
                                                                                     gcggcgATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGCCATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑AACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCCTTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGTAAAGAAGAAACTGATATTGCCCaa                                                                                                                                                        >  2:99487/1‑238 (MQ=255)
                                                                                     gcggcgATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCCTTAATCCCAAGAACGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACAGATAATGcc                                                                                                                                                           >  2:141533/1‑236 (MQ=255)
                                                                                     gcggcgATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCACGAGCGCTCCC‑TTGTTTGGCTTTTGGAAGGAGGAACCTGAAATTGCCCAt                                                                                                                                                        >  1:432378/1‑239 (MQ=255)
                                                                                     gcggcgATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTTCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCa                                                                                                                                                         >  1:849035/1‑238 (MQ=255)
                                                                                     gcggcgATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCAt                                                                                                                                                        >  1:65381/1‑239 (MQ=255)
                                                                                     gcggcgATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAAAAGAAAATGAAATTGCCCAt                                                                                                                                                        >  2:1118197/1‑239 (MQ=255)
                                                                                     gcggcgATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGCACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCACCGTTGCAACCAGCTGCATTAACCTTACA‑CCTAAGTTTCCTAACCCGCCATTAATACCAAGAGCGCTCCC‑TTGTTGTGCTTTTGGAAAAAAGaaaaagaaaataac                                                                                                                                                           >  2:508734/1‑225 (MQ=255)
                                                                                          gATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAAt                                                                                                                                                                                                               >  1:462230/1‑180 (MQ=255)
                                                                                               gggCAATCGCCAGTAGGGGTCCCCAATTCCATGCGGCATTCGACAGCGACATCCCCGAACCGTCGGCCTGCGGTCCGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCa                                                                                                                                              <  1:173461/238‑1 (MQ=255)
                                                                                                       ccccgtgggggTCCCCAAACCCTTGGGGCTTTCCCCAGCGACATCACCAAACCGCCGGCCTGCGTTCCGCC‑TTTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACCCTTACC‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGc                                                                                                                                      <  2:65381/230‑1 (MQ=255)
                                                                                                                 ttccccaactcacgggggatttCGCCAGCAACATCCCCGCACCGGCGGCCTG‑GGTTCGCCAATTGCCGCCGAAAAAGGCAAATAC‑AGG‑ACCAAAAATGC‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATAGTGCCCATGCTCGAAGCAAAGTTTGCACCTGCaaaa                                                                                                                            <  2:937317/219‑1 (MQ=255)
                                                                                                                  tACCAAAACCCATGCGGCATTCGCCAGCGACAACACCGAACCGCCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAA‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCaaaa                                                                                                                            <  1:40938/238‑1 (MQ=255)
                                                                                                                    cccAATCCCATCGGGAATTCGACAGCGAAATCACCGAACCGTCGGCAGGAGGTCCCAC‑ATTGCCGCCGAGAAAGGCAAATAC‑AGG‑TACAAAATTGA‑CCAGCGGTGCAACCAGCTGCATTACACTTCCT‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAAcc                                                                                                                          <  1:1006775/238‑1 (MQ=255)
                                                                                                                      cAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCA‑TTGGTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTACACCTGCAAAACCTCa                                                                                                                       >  1:1270236/1‑239 (MQ=255)
                                                                                                                         aTCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGGGCAACCAGCTGCATTACACTTACA‑CCCAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGGAAGAAAAAACTGATATTGCCCCTGCCCACAACAAAGGTTTCACCCGCAAAACCgaaaac                                                                                                                    >  2:929914/1‑234 (MQ=255)
                                                                                                                            cATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGACCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCaa                                                                                                                 >  1:91903/1‑239 (MQ=255)
                                                                                                                              tGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTTCACCTGCAAAACCGCATAGCaaa                                                                                                                >  1:907621/1‑238 (MQ=255)
                                                                                                                              tGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCa                                                                                                                                     >  1:975397/1‑217 (MQ=255)
                                                                                                                              tGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCaaa                                                                                                                >  1:749674/1‑238 (MQ=255)
                                                                                                                              tGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGACCCTGCAAAACCGctaaccaaa                                                                                                                >  1:809743/1‑230 (MQ=255)
                                                                                                                              tGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATCCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAACaaac                                                                                                               >  2:64476/1‑238 (MQ=255)
                                                                                                                              tGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCAGGCTCGAAGCAAAGTTTGCACCTGCAAAACAGCATAGCacac                                                                                                               >  1:740929/1‑236 (MQ=255)
                                                                                                                              tGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TAAAAAAATGA‑CCAGAGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTGGAAGCACAGTTTGCACCTTCAAAACCGCATAgacaa                                                                                                                >  1:50067/1‑234 (MQ=255)
                                                                                                                                  gCATTCGCCAGCGAAATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATAA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcga                                                                                                            <  1:879450/238‑1 (MQ=255)
                                                                                                                                   gtatCGCCAGCAACTTCCGCAAACCATGGGCCTGCGGAACGCC‑ATTGACGCTGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGAAACCAGCTGCATTACACTTACA‑CCGAAGTTACCTAAACCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGGTTGCACCTGCAAAACCGCATAGCAAAGcgat                                                                                                           <  1:816156/235‑1 (MQ=255)
                                                                                                                                     ttCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataac                                                                                                        >  1:713382/1‑239 (MQ=255)
                                                                                                                                            ggcaCATCACCGAACCTGCGGCCGGGGGTCCGCC‑ATTGCCCCCCAAAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTCCA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAat                                                                                                 <  1:564399/236‑1 (MQ=255)
                                                                                                                                            gccacctccccGAACCGTGGGCCTGCGGTCCGCC‑ATGGACCCCGAGAAAGGCAAATCC‑AGG‑TAAAAAAATGA‑CCAGCGGTGCAACCAGGTGCATTACCCTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAat                                                                                                 <  1:1303036/230‑1 (MQ=255)
                                                                                                                                              gaacccaccaaccctgcGGCTTGTGGTCCCCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAAGGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTCTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAatat                                                                                               <  2:1102625/223‑1 (MQ=255)
                                                                                                                                              gACATCACCGACCCGGGGGCCTGGGGATCCCA‑AGTGACGACGAGAAAGGAAAATAA‑AGG‑TACAAAAATGA‑CCTAGGGGGCACCAAACCGGATTACACTTCCG‑CCTAAGTTTCTTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCCCCTGCAAAACCGCATAGCAAAGCGATAACGATAAatat                                                                                               <  2:885529/239‑1 (MQ=255)
                                                                                                                                                      cgacctgTCGGCGTGCGGTACGCC‑ATGGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGTTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAg                                                                                       <  1:197399/233‑1 (MQ=255)
                                                                                                                                                      cGAACTGTCGTCCTGTGGACCGCC‑ATGGGCGCCGA‑AAAGGGAAAATC‑AGGTTACAAAAAGCA‑CCATCGGTGCACCCAGCTTCATTTCCCATCCA‑CCTAAGTGTCCTAATCCGACATTAATCCCAAGAGCGCTCCG‑TTGTTTGGCTGTAGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAg                                                                                       <  1:644400/239‑1 (MQ=255)
                                                                                                                                                         cccGTCGCCCTGGGGTTCCCC‑ATTGCCGCC‑AGAAAAGGAAAAACAAGG‑TAAAAAAAGTA‑CCAGCGGTGCAACCACCTCAATTCCCTTTCCC‑CCTAAGTTCCCTAATCCGCAATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAg                                                                                       <  2:927361/235‑1 (MQ=255)
                                                                                                                                                         aCCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAAAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACCCTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAgg                                                                                      <  1:1021473/237‑1 (MQ=255)
                                                                                                                                                          ggcgcggggtGCGGTACCCC‑TGTGTCGCCGGGAAAAGCAAATAC‑AGG‑TAAAAAAATGC‑ACAGAGTTGCAACCATCTGCATGACACTTACA‑ACTGAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAgg                                                                                      <  2:740929/227‑1 (MQ=255)
                                                                                                                                                             tCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATAGCCCAAAAGGAGAAt                                                                                 >  1:1377071/1‑238 (MQ=255)
                                                                                                                                                                gcatggggTACGCC‑ATGGCAGCCGAGAAAGGAAAATAC‑GAG‑TACAAAAAGTA‑CCAGCGGGGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTc                                                                               <  1:1301078/231‑1 (MQ=255)
                                                                                                                                                                   tGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTCCAAAACCGCATAGCAAAGCGAAAACGAAAAAAATACCAAAAGGAAGATTCggg                                                                            >  2:1021473/1‑236 (MQ=255)
                                                                                                                                                                     cGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGCTAAATATCCCAAAAGGAGAATTCGGAtttt                                                                        >  1:334867/1‑239 (MQ=255)
                                                                                                                                                                                ttGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAagag                                                                                                                                                                                                       <  1:843309/103‑1 (MQ=255)
                                                                                                                                                                                ttGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAagag                                                                                                                                                                                                       >  2:843309/1‑103 (MQ=255)
                                                                                                                                                                                ttGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGaaa                                                                                                                                                                      <  1:137655/135‑1 (MQ=255)
                                                                                                                                                                                ttGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGaaa                                                                                                                                                                      >  2:137655/1‑135 (MQ=255)
                                                                                                                                                                                   aCGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACC‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgat                                                                                                           <  1:68379/191‑1 (MQ=255)
                                                                                                                                                                                   aCGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgat                                                                                                           >  2:68379/1‑191 (MQ=255)
                                                                                                                                                                                        gagaAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTACGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGcc                                                      >  1:55323/1‑239 (MQ=255)
                                                                                                                                                                                        gagaAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGcc                                                      >  1:16468/1‑239 (MQ=255)
                                                                                                                                                                                             aGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGAAAAATATCCCAAAAGGAGAATTCGGATTTTGAACGGCAATTCCAAGCCAAACg                                                 >  2:445853/1‑239 (MQ=255)
                                                                                                                                                                                                cAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACC‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCa                                               <  1:1309553/238‑1 (MQ=255)
                                                                                                                                                                                                 tactAC‑AGC‑CACCAACATGA‑ACAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGCAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTTGGATTTTTCACGGAAATTACAAGcaaaaacccag                                             >  2:1367074/4‑229 (MQ=255)
                                                                                                                                                                                                 aaaTAA‑AGG‑TAAAAAAATAC‑CCGGGGGTGACACCGGCTCATTTTCCCTACCC‑CCAAATTTTCCTAATCCGCCATTAATCCCAAGAGCTCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAg                                             <  2:1067991/239‑1 (MQ=255)
                                                                                                                                                                                                   attc‑AGG‑TAAAAAATTGT‑CCAGCGTGGCAACCAGCTGCATTACCCTTCCC‑CCTAAGTTCCCTAATCCGCCATTAAGCCCAAGAGCCCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATAGCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGa                                           <  1:99487/236‑1 (MQ=255)
                                                                                                                                                                                                   aTAC‑AGT‑TACAAAAATGA‑CCAGCGGTGAAACCGGCTCCATTACCCTTACA‑CCAAATTTTCTGAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGa                                           <  2:749674/239‑1 (MQ=255)
                                                                                                                                                                                                   aTAC‑AGG‑TACAAAAATGC‑CCAGGGGTGCAACCAGCTGCATTACACTTACC‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGa                                           <  1:1118197/239‑1 (MQ=255)
                                                                                                                                                                                                              cAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGACTTCGGATTTTGCACGGCAATTCCGAGCCAGCAGCCAGGAATAATCAgg                                  >  1:355713/1‑239 (MQ=255)
                                                                                                                                                                                                                 aaaTGC‑CCAGCGGTGCAACCAGCTGCATTACCCTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAATAATCAGGa                                 <  1:906491/237‑1 (MQ=255)
                                                                                                                                                                                                                    tGA‑CCAGGGGTGCAACCAGCTGCATTACACTTACC‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAATAATCAGGATTGCa                            <  1:64476/239‑1 (MQ=255)
                                                                                                                                                                                                                    tGA‑CCAGGGGTACACCCAGCTTCATTACCCTTACC‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAATAATCAGGATTGCa                            <  1:1171837/239‑1 (MQ=255)
                                                                                                                                                                                                                    tGA‑CCAGCGGTGCAACCAGCTTCATTACCCTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAATAATCAGGATTGCa                            <  1:929914/239‑1 (MQ=255)
                                                                                                                                                                                                                     tgcccagggggcccaCCAGCTGCACTACACTTACG‑CCTAAGTTTCAAAATCCGCCATTAATCCCAAGAGCGCTCCC‑TGGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAATAATCAGGATTGCa                            <  2:809743/225‑1 (MQ=255)
                                                                                                                                                                                                                     tgcccAGCGGTGCAACCAGCTGCATTCCACTTACC‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAATAATCAGGATTGCa                            <  1:136985/236‑1 (MQ=255)
                                                                                                                                                                                                                     gA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGCTAAATATCCAAAAAGGAGAATCCGGATTTTGCACGGCAATTCCGAGCCAAAAGCAAGGAATAATCAGGATTACAg                           >  2:1172518/1‑239 (MQ=255)
                                                                                                                                                                                                                     gA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGTCAGACGAAAGGAATAATCAGGATTGCAg                           >  1:50294/1‑239 (MQ=255)
                                                                                                                                                                                                                      a‑CCGGCGGTGCAA‑CATGTTCATTTCACTCTCACCCTATGGTTCCAAAACCACCATTAAGCCCAAGAGCGCTCCC‑GTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAATAATCAGGATTGCa                            <  2:606951/237‑1 (MQ=255)
                                                                                                                                                                                                                      a‑CCAGCGGGGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGAATTCGGATTTTGCCCGGCAATTCCGAGCCCGTGCCAAGGAATAATCAGGATTGCAga                          >  1:565123/1‑238 (MQ=255)
                                                                                                                                                                                                                          aGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAAAATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAAAAATCAGGATTGCAGGAca                       >  2:924538/1‑238 (MQ=255)
                                                                                                                                                                                                                            cGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATATCGATAAATATCCCAAAAGGAGAATTCGGATTTTGCACGGCAATTCCGAGCACAACGCAAGGAAAAATAAGGATTTCAgaaaaaa                     >  2:906491/1‑233 (MQ=255)
                                                                                                                                                                                                                            cGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAATAATCAGGATTGCAGTACTaa                     >  1:606951/1‑239 (MQ=255)
                                                                                                                                                                                                                            cGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAATAATAAGGATTGCAGTACTaa                     >  1:225070/1‑239 (MQ=255)
                                                                                                                                                                                                                            cGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGAATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGAAAAAATCAGGATTGCAGTaacaa                     >  2:576838/1‑235 (MQ=255)
                                                                                                                                                                                                                                 cAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAATAATCAGGATTGCAGTACTAAAAAcc                >  1:896469/1‑239 (MQ=255)
                                                                                                                                                                                                                                            aTTACCCTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAATAATCAGGATTGCAGTACTAAAAACCGTCCATCGGc      <  1:141533/238‑1 (MQ=255)
                                                                                                                                                                                                                                             ttacccttaca‑cCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAATAATCAGGATTGCAGTACTAAAAACCGTCCATCGGCGt    <  1:334323/239‑1 (MQ=255)
                                                                                                                                                                                                                                                cacttaca‑cCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAATAATCAGGATTGCAGAACTAAAAACCGTCCATCGGCGTcc  >  2:332472/1‑238 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              
AGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGG‑CGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCC‑ATTGACGCCGAGAAAGGCAAATAC‑AGG‑TACAAAAATGA‑CCAGCGGTGCAACCAGCTGCATTACACTTACA‑CCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCC‑TTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTTTGCACGGCAATTCCGAGCCAGACGCAAGGAATAATCAGGATTGCAGTACTAAAAACCGTCCATCGGCGTCC  >  NC_000913/1543294‑1543766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A