Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,823,068 A→C 11.6% H204P (CAC→CCC)  nadE → NAD synthetase, NH3/glutamine‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,823,0680AC11.6% 243.9 / 2.1 117H204P (CAC→CCC) nadENAD synthetase, NH3/glutamine‑dependent
Reads supporting (aligned to +/- strand):  ref base A (53/40);  new base C (4/15);  total (57/60)
Fisher's exact test for biased strand distribution p-value = 5.30e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

AGGCATTGAACTGAGCGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATA  >  NC_000913/1822832‑1823298
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           
aGGCATTGAACTGAGCGATTTTGGTCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAacac                                                                                                                                                                                                                                            <  1:558917/238‑1 (MQ=255)
            gAGCGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTAACGTCTCAACAAACGT‑CAGGGAAAACAGTTACTTGCGGCCCTAGC‑TTCGCCCGAAAAACA‑TT‑Aaaaaaaaa                                                                                                                                                                                                                              >  2:1014137/1‑239 (MQ=255)
             aGCGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑TCCCGGAAAACC‑TT‑TTTAAGAAAg                                                                                                                                                                                                                             >  1:1218618/1‑238 (MQ=255)
             aGCGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGA‑TT‑GCCCGGGACACC‑TT‑TTTAAGAAAg                                                                                                                                                                                                                             >  1:566227/1‑238 (MQ=255)
              gCGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGT‑GGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGCCATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGGAAACAGTTACTGGCGGCATTAGC‑TT‑TCCCGGGACACC‑AT‑TATAAGAAAgcgc                                                                                                                                                                                                                          >  1:687746/1‑239 (MQ=255)
                  ttttGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGAAAACAGTAACTGGCGGCATTAGC‑TT‑GCCCCGAAAAAC‑CT‑TTTaaaaaaaggcaaa                                                                                                                                                                                                                       >  2:377576/1‑232 (MQ=255)
                      tgccGTGGCAATGAAAAAGCGCGTGGGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGc                                                                                                                                                                                                                  <  2:618529/237‑1 (MQ=255)
                       tCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CACTAAGGCGGCGGAGGCAATTACCGGATCCTTACCTAATACTGGTGACGACGTGACGGACATTAATCGCCGGTTTC‑TCACCAAAAAAGTCCAGGGTAAAAAGTACTTGCCGCCATTACA‑TT‑GCCTGCAACAAC‑TTATAAAAAAAAG‑GCC‑AACGGc                                                                                                                                                                                                                  >  2:682642/1‑238 (MQ=255)
                       tCCGGGGAAATGAAAAAGCGCGTGGGGGGGTGAAAGCCCAATATAGCGTTGCGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGcc                                                                                                                                                                                                                 <  2:1218618/239‑1 (MQ=255)
                            ggCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACCTTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATAAGC‑TT‑GCCCCGAACCCC‑TT‑TTTaaaaaaagcccaaaagccaggtg                                                                                                                                                                                                             >  2:591000/1‑222 (MQ=255)
                                 tGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGCACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAg                                                                                                                                                                                                      >  1:736231/1‑239 (MQ=255)
                                          gggTGAGCGGATGAAAGCACAATAGAGCATTACGGGTATGAACAGCGGTGTCGTGGTGGGCAC‑CGGTCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCg                                                                                                                                                                                              <  1:516259/235‑1 (MQ=255)
                                           cgTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATTGc                                                                                                                                                                                             >  1:256525/1‑237 (MQ=255)
                                                gCGGATGAAAGCAAAAAATCGCATTGGGGGTATGCCCGGCGGTTGCGGGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTtct                                                                                                                                                                                       <  2:1232667/238‑1 (MQ=255)
                                                cgggATGAAAGCACAATATGCCATTGCGGGTATGACCAGCGGGGTCTGGGTGGGCCC‑CGATCATGCAGCAAAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCACCAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTtct                                                                                                                                                                                       <  2:1184757/236‑1 (MQ=255)
                                                    aTGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGc                                                                                                                                                                                   <  1:377576/238‑1 (MQ=255)
                                                                tataGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGc                                                                                                                                                                      >  1:125023/1‑239 (MQ=255)
                                                                        tGCGGGTTTGCCCCGGGGTGTCGTGGT‑GGCCC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGt                                                                                                                                                             <  2:687746/239‑1 (MQ=255)
                                                                        tGCGGGTATGACCAGCGGTGTCGTGGGGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAGCAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCg                                                                                                                                                              <  1:570434/239‑1 (MQ=255)
                                                                        tGCGGGTAGGACCAGCGGTGTCGTGGTGGGAAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCg                                                                                                                                                              <  1:801787/239‑1 (MQ=255)
                                                                        gttataaggaacaggCGGGGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCg                                                                                                                                                              <  1:1361627/225‑1 (MQ=255)
                                                                        gggggtgtggACCGGGGGTGTCGTGGTGGGCCA‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATAGGGTGAAGGCGGTACGGACATTAAGCCGCTGGATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCg                                                                                                                                                              <  2:28947/230‑1 (MQ=255)
                                                                        ggCGGGTATGAACAGCGGTGTCTTGGTGGGCAC‑CGATCCTGCCGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGTATTAGC‑TT‑GCCCGGAACTCC‑TT‑TATAAGAAAGCGTC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCg                                                                                                                                                              <  1:829271/238‑1 (MQ=255)
                                                                          cGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTCAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAGCAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TTTAAGAAAGACCC‑AACAGACGATC‑TGGAGAA‑AGAACGCCCTTTTCTGcccagagaaaggggcaatgggtgg                                                                                                                                                            >  2:570434/1‑217 (MQ=255)
                                                                          cGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TTTAAGAAAGCGCC‑AACGGCCGACC‑TGGAAAA‑TGATCGCCCTTCTCTGCCAGCTAAAGTGGCACCCGGCGt                                                                                                                                                             >  1:1050027/1‑238 (MQ=255)
                                                                          cGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGGGCC‑AAACGCCGACC‑TGGAAGA‑GGAACGCCCTCCTCTCCCCGAGGaaagggacaccgcgggt                                                                                                                                                            >  2:799578/1‑224 (MQ=255)
                                                                          cGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCAATTGG‑CT‑GCCCGGGACACC‑TT‑TTTAAAAAAAGGCC‑AACGGCCGAACGTGAAAGA‑T‑AACCCCCCTCTCTGCCAGGAGAAGAGGGACCCgggggg                                                                                                                                                            >  2:903264/1‑235 (MQ=255)
                                                                          cGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGGAAACAGTTACTGTCGGCCTTAGA‑TT‑TACCGGAAAACC‑CT‑TTTAACAAAGCGCC‑AAAGGCCGACC‑TGGAAAATTGACCGCCC‑TCTCCGCCCAATTAAGGGGCAccccgaggg                                                                                                                                                            >  2:244558/1‑231 (MQ=255)
                                                                          cGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGCAAACAGTTACTGGAGGCATTTGC‑TT‑GCCCGGGACCAC‑TT‑TATTAGAAAGCGGC‑CACAGCCCCAC‑TGGGAGA‑TGATCGCCCTTCTCTTCCCCATGAAGAGGCAccagggggg                                                                                                                                                            >  2:249051/1‑231 (MQ=255)
                                                                          cGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACAGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑TCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGCTC‑TGGAACA‑TGAACGCCCTTTCCTTCCCGATGAAGTGGcctcgcgcctg                                                                                                                                                            >  1:1363977/1‑229 (MQ=255)
                                                                              taggACCAGGGGTTTCTGGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGAcc                                                                                                                                                         <  1:903264/235‑1 (MQ=255)
                                                                              tATGACCAGCGGTGGCGTGGTGGGCCG‑CGATCATGCAGCAGAAGCCATTACCGGATTCGTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGAcc                                                                                                                                                         <  1:259104/238‑1 (MQ=255)
                                                                                     ccggggtgtgtggtgGGGACACGTTCAGGCGGCAGAAGCCATTACCGGTTTTTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACa                                                                                                                                                  <  2:398549/230‑1 (MQ=255)
                                                                                       cGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAATGGCACTCGGCGTGACCTATGACAata                                                                                                                                               >  1:1030041/1‑239 (MQ=255)
                                                                                             cGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATcgac                                                                                                                                          <  1:941616/238‑1 (MQ=255)
                                                                                             cGTGGGGGGCAC‑CGAGCATGCAGGAGAAGCCATTTCCGGATTTTTCACTAAATATGGTGACGGGGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAAGAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AGCGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATcgac                                                                                                                                          <  1:619290/238‑1 (MQ=255)
                                                                                                 gtggGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGGACGGACATTAATCCGCTTGATCGTCTCAACAAACGT‑CAGGGGAAACAGTTACTGGGCGCATTTGCTTT‑CCCCGAAACACC‑TT‑T‑TACGAAAACGCC‑CACGGCCGAAC‑TGGGAGA‑TGATGGCCCCTTTCTCCCCACTGAAAGGGCACCCCGCCGGACCCATGaaccacccctttaaaa                                                                                                                                     >  2:1361627/1‑224 (MQ=255)
                                                                                                    ggCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACACAATCGACGACTAttt                                                                                                                                  >  1:291545/1‑237 (MQ=255)
                                                                                                      cAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAACGCC‑CAACGCCGATC‑TGGAAGA‑TGAACCCCCTTCCCTCCCAAATGAAAGGGGACCCCGCGGGGCCTATGaaaaataccaacgcaaattgg                                                                                                                                >  2:1324593/1‑222 (MQ=255)
                                                                                                      cAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAAACCC‑TT‑TATAAGAAAAGGCCAAACGGCC‑ATC‑TGGAAGG‑TTATCGCCCTTTTCCTCCAAATGAAGGGGCACCCGGCGGGGCCCATGAAAAAAAcgacacaccactgg                                                                                                                                >  2:1275258/1‑229 (MQ=255)
                                                                                                        c‑cGGTCATGCAGCAGAAGCCATTACCGGATTCTTAACTAAATATGGTGACGGCAGTACGGACCATAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGTACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGaa                                                                                                                              <  1:989324/239‑1 (MQ=255)
                                                                                                         cgcatcatgccggttaaGGCATTTACGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAAGCCGCTGTATCGTCTCAACAAACGT‑CAGGGGAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGACCACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGGCCTATGCCAATATCGACGACTATCTGGaa                                                                                                                              <  2:566227/224‑1 (MQ=255)
                                                                                                                   agcagTAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGaaaaa                                                                                                                      <  1:143597/237‑1 (MQ=255)
                                                                                                                    gcagAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTTTGCCAGGTGAAGTGGCACTCGGCGGGACCTATGACAAAATCGCCGACTATCTGGAAGGGGAAAACg                                                                                                                    >  1:803377/1‑238 (MQ=255)
                                                                                                                      agAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACgga                                                                                                                  >  1:192366/1‑236 (MQ=255)
                                                                                                                         aGCCAGTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCt                                                                                                               <  1:558012/238‑1 (MQ=255)
                                                                                                                              tttccGGTTTTTTTACAAAAAATGGGGGCGGGGGTACGGACATTAACCCGGTGGTACGTCCCAACAAACGT‑CGGGGTAAAGAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TAAAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAg                                                                                                         <  2:17910/236‑1 (MQ=255)
                                                                                                                                   ggATTTTTCCCTAAATTTGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGc                                                                                                    <  1:1014137/239‑1 (MQ=255)
                                                                                                                                   ggATTTTTCACTAAATAGGGGGGCGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGc                                                                                                    <  1:1046130/239‑1 (MQ=255)
                                                                                                                                   ggATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGc                                                                                                    <  1:249051/239‑1 (MQ=255)
                                                                                                                                      ttcttcACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGCCGACTATCTGGAAGGGAAAAACGTACCCCAACAGGTCGcccg                                                                                                 >  1:1280052/1‑237 (MQ=255)
                                                                                                                                       tcttcACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAGCAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGAACGCCCTTCTCTGCCAGATGAAGGGGCACTCTGCGTGACCTATGACCAAATCCGAGGCGACCTGGAAGGGAAAAACACACCCCAAAAAGATGCCAGa                                                                                                >  2:530271/1‑239 (MQ=255)
                                                                                                                                        tttcACTAAATTTGGGGACGGGGGTACGGACATTAATCCGCTGTATCGTCCCAAAAAACGT‑CAGGGTAAACAGTTCCTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGaa                                                                                               <  2:1280052/238‑1 (MQ=255)
                                                                                                                                        tttcACTAAAATGGGGGACGGGGGTAAGGAAATTAATCCCCTGTTCTGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGaa                                                                                               <  2:192366/238‑1 (MQ=255)
                                                                                                                                        cttcACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGaa                                                                                               <  1:1022671/239‑1 (MQ=255)
                                                                                                                                             atAAATCTGGAGGCGGCGGTACGGACAGTTATCCGCTTGAACGGCTAAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCCGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATa                                                                                          <  1:871286/238‑1 (MQ=255)
                                                                                                                                              tAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATag                                                                                         <  1:799578/239‑1 (MQ=255)
                                                                                                                                                  tatGGTGACGGGGGTAAGGTCTTTAATCCGCTGTATCGTTTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TG‑GCCCGGAACGCC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGaa                                                                                     <  2:889718/239‑1 (MQ=255)
                                                                                                                                                     ggTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCACTAGC‑TT‑GCCCGGAACACC‑TT‑TTTAAAACAGCGCC‑AACCGCCGATC‑TGGAAGA‑TCATCCCCCTTCTCTGCCAGCTGAAGCGGCACCCGGCGTGACCCTTGACAAATTCCCCACCTTCCTGGAGGGGAAAAACGTCCCCCAACCAGTCGCCCGAACaaaaacgaaacg                                                                                  >  2:445707/1‑229 (MQ=255)
                                                                                                                                                       tGACGGCGGTACGGACATTAATCCGCTGTATCGTGTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTgg                                                                                 <  1:246047/238‑1 (MQ=255)
                                                                                                                                                        gACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCCCTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCCGAACAATAGAGAACTggga                                                                               >  1:372125/1‑237 (MQ=255)
                                                                                                                                                        gACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCCCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAAAAATAGAGAACTGGTa                                                                               >  1:464980/1‑239 (MQ=255)
                                                                                                                                                                         tAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAAAATa                                                              >  1:809278/1‑239 (MQ=255)
                                                                                                                                                                                 tGTATCTTCTCAACAAACGT‑CAGGGTAAACAGTTACGGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGt                                                      <  1:752521/239‑1 (MQ=255)
                                                                                                                                                                                 tGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACCATATCGCCGACTATCTTGAAGGGCAAAACGTACCTCCACAGGTCCCCCGAACCATAGAGAACTGGTATCTGGAAACCGGACATAAACCCCg                                                       >  1:619041/1‑238 (MQ=255)
                                                                                                                                                                                 tGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTGGCCAGAACAATAGAGAACTGGAAACTGAAAACCGAACATAAACGCCg                                                       >  1:314613/1‑238 (MQ=255)
                                                                                                                                                                                 tGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAACGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCCCAACAGGTCGCCCGAACAAAAGCGAACTGGTAACTGCAAACCGCACATAAACGcag                                                       >  1:510305/1‑236 (MQ=255)
                                                                                                                                                                                  gtttCGTCCCAACAAACCG‑AAGGGGAAACAGTTACGGGGGGCATTAGG‑TT‑GCCCGGAACCCC‑TT‑TAAAAGAAAGCGCC‑AACGGCCGATC‑CGGAAGA‑TGATCGCCCTTATCTGCCAGATGAAGTGGCACTCGGCGTGCCCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTc                                                     <  2:699913/236‑1 (MQ=255)
                                                                                                                                                                                  gTAACGTCTCAACAAACGG‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACCCC‑TT‑TATAAGAAAGCCGC‑AACGGCCGATC‑GGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTTACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGt                                                      <  1:682642/238‑1 (MQ=255)
                                                                                                                                                                                   tATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCCGGACCTATGACAATATCGCCGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTTGCCAGAACAATAGAGAACTGGGATCTGAAAACCGAACATAAACGCCGTc                                                     >  1:717162/1‑238 (MQ=255)
                                                                                                                                                                                        tctcAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGGGACCTATGACAAAATCGACGACCATCTGGCAGGGAAAAACGGACCTCAACAGGTCGCCCGAACAATAGAGAACAGGAATATGAAAACCCAAAAAAAACGCCGGCCGCCaa                                               >  2:302777/1‑239 (MQ=255)
                                                                                                                                                                                        tctcAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACCGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGGGACCTATGACAATATCGACGACTATATGGAAGGGGAAAACGTACCCCACCAGGTCGCCCGAACAAAAGAGAAATGGAATTTGAAAACCCaaacaaaacccccacccccaa                                               >  2:1055249/1‑220 (MQ=255)
                                                                                                                                                                                                         ggTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAAAACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATAGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACCATTTGGAAGGGAAAAAAGTACCTCTACAGGTCGCACGAACAATAGAGAAATGGTATCTGAAAACCCAACAAAAACCCCGCACCCCAAATACCGGTTTCgagta                               >  2:918170/1‑236 (MQ=255)
                                                                                                                                                                                                         ggTAAACAATTACTGGGGGCGGTAGC‑TG‑GCCCGGAAACCC‑TT‑TAAAAGAAAGCGCC‑ACCGGCCGATC‑GGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCTGGACCTATGACAATAGCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCgat                                 <  2:617505/237‑1 (MQ=255)
                                                                                                                                                                                                                 gTTACTGGGGGCTTTAGC‑TT‑GCCCGG‑ACACCTTT‑TATAAGAAAGGGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGa                       <  2:125023/239‑1 (MQ=255)
                                                                                                                                                                                                                  ttACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGGAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGGACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAAACGAACAAAAACGCCGGTCGCCAATTAACGTTTTGCTTGATTTCTGGaa                      >  1:602096/1‑239 (MQ=255)
                                                                                                                                                                                                                   tACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCCCTCGGCGGGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAAAAGGGCGCCCGAAAAATAAAGAACTGGGATTTGAAAACCCAACATAAAAGCCGCCCCCCAATTACCCGTTTCGTTGATTTTTGGaaa                     >  2:1328417/1‑239 (MQ=255)
                                                                                                                                                                                                                   tACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGa                       <  1:845876/237‑1 (MQ=255)
                                                                                                                                                                                                                   tACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGGGACCTATGACAATATCGCCGACTATCTGGAAGGGAAAAACGTACCTCAAAAGGTCGCCAGAACAATAGGGAACTGGTATCTGAAAACCGAACATAAAAGCCGTCCCCCAATTAACGTTTTCTATGATTTCTGGa                       >  1:617505/1‑237 (MQ=255)
                                                                                                                                                                                                                    aCTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGGCCTATGACAATATCCACGACTATCTGGACGGGAAAAACGTACCCCAAAAGGTCGCCAGGACAATAGAGAACTGGAATCTGGAAACCGAACATAAACGCCCTACGCCACTTACCAGTTTCGTGGAATTTTGGaaaa                    >  2:1187911/1‑239 (MQ=255)
                                                                                                                                                                                                                          ggcATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGGGAACTGGTATCTGAAAACCGAACATAAACCCCGGCCGCCAATTACCGTTTTCGCTTATTTCTTGAAAAAGcaaa              >  1:736889/1‑238 (MQ=255)
                                                                                                                                                                                                                            cATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGGGGAACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGGACTGGGAACTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGGTTTCGATGATTTCTGGAAAAAGtaataa            >  1:192589/1‑239 (MQ=255)
                                                                                                                                                                                                                            cATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAATGGCACTCGGCGGGGCCTATGACAATATCGACTGCTATCTGGAAGGGGAAAACGAACCCCAACAGGGCGCCAGAACAATAACGAACTGGAGACTGAAAACCGACAATAAACCCCCACCCCCAAATACAGTTTTCGTTATATTTTGGAAAAAGatataa            >  2:198453/1‑239 (MQ=255)
                                                                                                                                                                                                                              ttAGC‑TT‑GCCCGGAACCCC‑TT‑TATAAGAAGGGGCC‑AACGGCCGATC‑TGGAAGA‑TGAACGACCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGAAAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTaataat           <  1:432420/238‑1 (MQ=255)
                                                                                                                                                                                                                                aGC‑TT‑GCCCGGAACTCC‑TT‑TTAAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCGCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATAGCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTg        <  1:348312/239‑1 (MQ=255)
                                                                                                                                                                                                                                aGC‑TT‑GCCCGGAACCGC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCGCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTg        <  1:1324593/239‑1 (MQ=255)
                                                                                                                                                                                                                                aGC‑TT‑GCCCGGAACACC‑TT‑TATCAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACCCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGG‑AAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGc       <  1:475440/239‑1 (MQ=255)
                                                                                                                                                                                                                                 tgttt‑GCCCGGAACCCC‑TT‑TATAAGAAAGCGCC‑AAAGGCCGATC‑TGGAAGA‑TGAACGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGT‑CGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGc       <  2:619041/236‑1 (MQ=255)
                                                                                                                                                                                                                                 tc‑TT‑GCCCGGAACTCC‑TT‑TATAAGAAAGCGGC‑AACGGCCGATC‑GGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGc       <  1:741466/238‑1 (MQ=255)
                                                                                                                                                                                                                                 gC‑TT‑TCCCGGAACCCT‑TT‑TTTAAAAAAGCGCC‑AACCGCCTATC‑TGGAAGG‑TGATCGCCCTTCTGTGCCAGATGAAGTGGCAATCGGCGGGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGc       <  2:489917/239‑1 (MQ=255)
                                                                                                                                                                                                                                 gC‑TT‑GCCCGGAACCCC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGc       <  1:1328417/239‑1 (MQ=255)
                                                                                                                                                                                                                                 gC‑TT‑GCCCGGAACCCC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGGGGCACTCGGCGTGACCTATGACAATAGCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGc       <  1:445707/239‑1 (MQ=255)
                                                                                                                                                                                                                                 gC‑TT‑GCCCGGAACCCC‑TT‑TAAAAGAAAGCGAC‑AACGGCCGATC‑GGGAAGA‑TGATCGCCCTTCTCGGCCGGATGAAGGGGCACTCGGCGTGACCTAAGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGc       <  2:1363977/239‑1 (MQ=255)
                                                                                                                                                                                                                                 gC‑TT‑GCCCGGAACACC‑TT‑TAAAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGc       <  1:244558/239‑1 (MQ=255)
                                                                                                                                                                                                                                  c‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGtaata             >  1:359562/1‑232 (MQ=255)
                                                                                                                                                                                                                                   gctgtcccGGAACTCC‑TT‑TAAAAAAAAGCCCC‑AACGGCCGATT‑TGGAACA‑TTGTGCCCCTTCTCTTCCAGATGAAGTGGCCCTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGc       <  2:840497/234‑1 (MQ=255)
                                                                                                                                                                                                                                    tt‑GCCCGGAACCCC‑TT‑TAGAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGcac     <  1:1205084/239‑1 (MQ=255)
                                                                                                                                                                                                                                    tt‑GCCCGGAACCCC‑TT‑TAAAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGc       <  1:106373/237‑1 (MQ=255)
                                                                                                                                                                                                                                        cccGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGAAGATTTCTGGAAAAAGTAATAAttttcccaaa  >  1:637046/1‑232 (MQ=255)
                                                                                                                                                                                                                                        cccGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTAACTCAACAGGTCGCCCGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCCCCAATTACCGTTTTTGATGATTTCTGGAAAAAAAAAAAATTTTCAAata  >  2:1205084/1‑239 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           
AGGCATTGAACTGAGCGATTTTGTCCGTGGCAATGAAAAAGCGCGTGAGCGGATGAAAGCACAATATAGCATTGCGGGTATGACCAGCGGTGTCGTGGTGGGCAC‑CGATCATGCAGCAGAAGCCATTACCGGATTCTTCACTAAATATGGTGACGGCGGTACGGACATTAATCCGCTGTATCGTCTCAACAAACGT‑CAGGGTAAACAGTTACTGGCGGCATTAGC‑TT‑GCCCGGAACACC‑TT‑TATAAGAAAGCGCC‑AACGGCCGATC‑TGGAAGA‑TGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATA  >  NC_000913/1822832‑1823298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A