Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,825,217 G→T 10.6% Q137K (CAA→AAA)  spy ← periplasmic ATP‑independent protein refolding chaperone, stress‑induced

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,2170GT10.6% 388.7 / 2.4 136Q137K (CAA→AAA) spyperiplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (52/65);  new base T (16/2);  total (69/67)
Fisher's exact test for biased strand distribution p-value = 6.01e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01

Only 100 of 137 total aligned reads displayed.

ACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGT  >  NC_000913/1824980‑1825450
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           
ccggggggtgtttacgggCAACTGGGTTGTTTTAGGTTAGTGGTGATGGGGTTTTTTTAACACTTGTTGGAAAAGAAAAAAAAAA‑CCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGc                                                                                                                                                                                                                                            <  1:1200880/223‑1 (MQ=255)
  cGTGGATGGTTATCGGCAACTCGGGTGATTGACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGAAGTGGACAAGACCGCGGGCTCTTCGGTTTTTTCG‑TTAAGGATTATTTAGCAGGTGGAGGGACTTTCTCTTTTTGCCCTGGGCGTTCTGTTAA‑ACCCTCCTCAAAAATA‑G‑A‑AATAAAATTCtt                                                                                                                                                                                                                                          >  2:825722/1‑239 (MQ=255)
       aTGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGCCAAGACCG‑GCGGTCTTAAGTTTTTTTGCTGAAAGATTTTTTAGCCGTTTCAGGCATTTTACCTTTTTCGCCCGGAGGTTCTGTCCG‑AGCGTTTTCCAAATTA‑G‑C‑ATTCAATTGtttttttt                                                                                                                                                                                                                                     >  1:1275387/1‑237 (MQ=255)
           gttcgcgaactgggggttgtttACGTGTGTGGTGATCGTGTTTTTTTAACATCTTTGAGAGAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGtt                                                                                                                                                                                                                                  <  1:1355270/219‑1 (MQ=255)
                 gacacgtggtggATTTGCTTTGGTGGTGATCGGTTTTTTTTTACACGTGTTAGAGAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGt                                                                                                                                                                                                                           <  2:40510/228‑1 (MQ=255)
                     cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGGTTTTTTGCTGAAAGATTATTTAGCAGTTGCCGGCATTTTACCTTTTTCCGCTGGACGTTTTTTTAG‑ACCCGTCTCAAAATTA‑GAC‑TTTAAATttttttttttttctgccggcacg                                                                                                                                                                                                                        >  2:136085/1‑224 (MQ=255)
                     cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGGCTTAAGTTTTTTGGCTGAAAGATTATTCCGCCGGTGCAGGCATTTTTCCTTTTTCCGCTGGACGGTCTGTCAG‑AAGCCTCTCAAAATTATG‑A‑TTTAATTTGCTTTTTTTTTTCCgggccagg                                                                                                                                                                                                                        >  2:1114894/1‑233 (MQ=255)
                                tACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGAATATTCAGCAGTTGCAGGCCTTTTACCTTTTTCCGCCGGACGTTTTGTCAG‑ACGCTTCTCAACATTA‑G‑C‑ATTAAATTTCTTTTTTTTTTTCGCCGGCAGGAGGTT‑GTaaaa                                                                                                                                                                                                           >  1:1357860/1‑238 (MQ=255)
                                tACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGCCTTAAGTTTTTTTGCCGAAAGATTATTCAGCAGTTTCAGGCCTTTTACCTTTTGCCGCTGGACGGTCTGTCAG‑AAGCTTCTCCAAATTA‑T‑C‑CTTTAATTTCTTTTTCTGTTCCGGCGTCAGGTGTTT‑GTaaaa                                                                                                                                                                                                           >  1:1240299/1‑238 (MQ=255)
                                      tgttttgacttgtttttttttACATTGGTTGGAAAAAAAAAAAAAAA‑CCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGtt                                                                                                                                                                                                     <  2:1098981/225‑1 (MQ=255)
                                        tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAACAGTTTCCGGCCTTTTACCCTTTTCCGCTTGGAGTTTTGTCAT‑ACGCGTCTCAAAATTA‑G‑C‑ATTAAATTTCTTTTTTTTTTCCGGCGCCGGGCTGTT‑GTAAAtttttt                                                                                                                                                                                                      >  2:1095947/1‑234 (MQ=255)
                                        tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGGAAGAGTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGGAGTTTTGTCAG‑ACGGTTCTCAAAATTA‑G‑C‑ATTAAATTTCTTTTTCTGTTTCGGCGTCAGGAAGTT‑GTAAATTTTGt                                                                                                                                                                                                      >  1:951036/1‑236 (MQ=255)
                                                   ttttttttACAATTGTTTGAAAAAAAAAAAAAAACACCGCCCATCCTGTCCACCTCATTACTGGAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCAt                                                                                                                                                                                        <  2:816048/238‑1 (MQ=255)
                                                    tttttttACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCATCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAAGTTTTTGCCTGGAAGATTATTCATCGCTTGGAGGCATTTTATCTTTTTCCGCAGGCGGTTTTTTTAG‑ACGATTGCCCAAATTA‑T‑C‑ATTAAATTGCTTTTTCTGTTCCTGCGCCAGGAGGTT‑TTACATTTTGTGTT‑GGGGTTcctgtt                                                                                                                                                                                      >  2:741709/1‑235 (MQ=255)
                                                                ttttagaaaaagaaaaaaaaa‑aCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGt                                                                                                                                                                          <  1:1065352/226‑1 (MQ=255)
                                                                ttgaaaaaaaaaaaaaaaaaa‑cCCGCCGATCCTGTCCCCCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGt                                                                                                                                                                          <  2:159469/226‑1 (MQ=255)
                                                                  tttGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTTAGTTTTTTTGCTGAAAGATTATTCAGCAGGTGGAGGGATTTTTACTTTTTCCGCTGGACGGTTTTTCAG‑AAGCTTCTCAAAATTA‑A‑CAATAAAATTTCTTTTT‑TTTTTTGGCGGCAGGATGTT‑GTAAAATTTTTTTT‑GGGGTTTCAtttggggccccatggta                                                                                                                                                                        >  2:1006902/1‑226 (MQ=255)
                                                                    tGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTTGGTGAAAGATTATTCAGCAGTTGCAGGCATTTTATCTTTTTCCGCCGGACGTTCTGTCAT‑GAGCGTCTCAAAATTT‑G‑C‑ACTAAAATTCTTTTTTTTTTTCGGGGGCCGGAGTTT‑GTAAAATTTGTTCT‑GGGttttccttttggcccccaatttg                                                                                                                                                                        >  2:146550/1‑217 (MQ=255)
                                                                    tGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGCAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTAGGTGAAAAATTATTCCGCAATTGCACGCATTTTACCTTTTTGCGCTGGACGGTCTTTCAG‑ACGCTTCCCCAAATAA‑G‑C‑ATTAAATTGGTTTTTTTTTTCCGGGCTCCGGTTTTT‑TATAATTTTTTTTT‑GGGGTTCCCTTGGGGCCCGCATTTTAgg                                                                                                                                                                      >  2:1336437/1‑238 (MQ=255)
                                                                         agaaaaAAGAAA‑TCCGGCGGTCCTGTCCACCGGATTTCGGCAAGGTGGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTgc                                                                                                                                                                 <  2:32856/239‑1 (MQ=255)
                                                                          aagaaaagaaa‑CCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTgcg                                                                                                                                                                <  1:330609/238‑1 (MQ=255)
                                                                          aaaaaaaaaaa‑aCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTGTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTgcg                                                                                                                                                                <  1:1174510/236‑1 (MQ=255)
                                                                                               ccTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCg                                                                                                                                             <  1:278358/238‑1 (MQ=255)
                                                                                                ctttCCCCCGCATTACTGCAAGGTAGTGGACAAGACCG‑GGGGTTTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGAt                                                                                                                                           <  2:1228476/236‑1 (MQ=255)
                                                                                                cTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGAt                                                                                                                                           <  1:1095947/239‑1 (MQ=255)
                                                                                                  gTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTTGCTGGAAGATTATTCAGCAGGTGCAGGCCTTTTACCTTTTTCCGCTGGACGGTCTGTCAA‑ACGCTTCTCAAAAATA‑A‑C‑CATTAATTTCTTTTTTTTTTCCCGCGTCCTGTGGTT‑TTAAAATTTGTTTT‑GGGGTTTCCTGTTCCCCAGCAAGGTAGCTTTTCCGTTTTTTTCCCtttttttggttt                                                                                                                                         >  2:921162/1‑232 (MQ=255)
                                                                                                   tcccacGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTg                                                                                                                                        <  1:1336437/234‑1 (MQ=255)
                                                                                                        cGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTTTTAAGTTTTTTGGCTGAAAGATTATTTCGCAGTTGGAGGCATTTTACCTTTTTTCGCTGGGAGGTCTTTCCG‑ACGCTTTTCAAAATTA‑A‑C‑CTTTAATTGCTTTTTCTGTTCCTGCGGCCGGGGGTTGGTAAATTTT‑TTCT‑TGGTTTTCCTTTGGGCCCGCCGGTTAGCTTTGCGCTGTTTTTTCAtttttttggtatggggt                                                                                                                                    >  2:691631/1‑226 (MQ=255)
                                                                                                        cGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTTGCTGGAAGATTATTTAGCAGTTGCAGGCCTTTTTCCCTTTTCCCCCGGACGTTCTTTCCG‑ACGCTTTTCAAAATTA‑G‑A‑CTTTAATTGCTTTTTTTGTTCCCGCGTCCGGATGTT‑TTAAATTTTGTTTT‑TGGTTTTCCTTTTCGCCAGCCTTTTGACTTTTGCCTGTTTTTCCATTTTTTCGATTTGCGCt                                                                                                                                    >  2:582178/1‑238 (MQ=255)
                                                                                                        cGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATTTGCGCCAGCATGTTAGCTTTTCGCTGTTCTTCCATTTTTTCGGTCtcggct                                                                                                                                    >  1:1114478/1‑233 (MQ=255)
                                                                                                        cGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGCCTTTTGTTTTTTGGGTGAAAGGTTATTCAGCAGATGGAGGCATTTTTCCTTTTTCCGCTGGCCGGTCTGTCAG‑ACACTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTTTGTTTCGGCGTCAGGGTGTT‑GTAAATTTTTTTTT‑GGGTTTTCATTGTCGCCCGCATGTTAACTTTGAGCTGTTTTTCCATTTTTTTGATCTgcggt                                                                                                                                    >  2:932770/1‑236 (MQ=255)
                                                                                                         gCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTc                                                                                                                                  <  1:574006/239‑1 (MQ=255)
                                                                                                             tACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCCTTTTACCTTTTTCCGCTGGACGTTCTGTTAA‑ACGCTTCTCCAAATTA‑G‑C‑ATTTAATTGCTTTTTTTGTTCCGGCGTCAGGATGTT‑GTAAATTTTTTTTT‑GGGTTTTCTTTTGGGCCCGCATGTTAGCTTTGCGCTGTTTTTCCATTTTTTCGATTTGCGCTTCCGCt                                                                                                                              >  2:471777/1‑239 (MQ=255)
                                                                                                               cTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGGTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTTCATTTTTTCGGTCTGCGCTTCAGCttt                                                                                                                            >  1:753614/1‑239 (MQ=255)
                                                                                                                   aaGGTAGTGGACAAGGCCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACt                                                                                                                        <  1:541928/239‑1 (MQ=255)
                                                                                                                       tAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCAttttt                                                                                                                                                <  1:850627/211‑1 (MQ=255)
                                                                                                                        tgTGGACAAGACCG‑GCGGTCTTAAGTTTTGTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCGGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTAt                                                                                                                    <  1:435126/237‑1 (MQ=255)
                                                                                                                          tGGACAAGGCCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGa                                                                                                                 <  1:471777/239‑1 (MQ=255)
                                                                                                                          tGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTTTCAAAATTA‑G‑C‑ATTAAATTGCCTTTTTTGTTCCGGCGTCAGGATGTT‑GTAAACTTTGTTCT‑TGGTTTCCATGTCGGCCAGCATGTTAGCTTTGCGCTGTTCTTCCCTTTTTTCGATTCTCCCTTCCCCCTTTACTTTTTcta                                                                                                                 >  1:1043413/1‑237 (MQ=255)
                                                                                                                          tGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTTAATTGCTTTTTTTGTTCCGGCGTCAGGGTGTT‑GTAAATTTTGTTTT‑TGGTTTTCCTTTGCGCCCGCCTGTTTGCTTTTGGCTGTTTTTTCCTTTTTTCGACTTGCGCTTCACTTTTTACTTTTTCGa                                                                                                                 >  2:1124232/1‑239 (MQ=255)
                                                                                                                          tGGACAAGACCG‑GCGGTCTTAAGGTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGCTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTTTGTTCCGGCGTCAGGATGTT‑GTAAATTTTTTTCT‑GGGGTTCCATGTGCGCCAGCATGTTAGCTTTTCGCTGTTCTTCCATTTTTTCGGTCTGCGCTTCCGCTTTTACTTTATCGa                                                                                                                 >  1:744947/1‑239 (MQ=255)
                                                                                                                            gACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGcc                                                                                                                                                                                 <  1:451964/173‑1 (MQ=255)
                                                                                                                               aaGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTTT‑GGGTTTTCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTTCGATCTGCGCTTCAGCTTTTACTTTTTCGAAGGt                                                                                                             >  1:309303/1‑238 (MQ=255)
                                                                                                                                 gACCG‑GCGGTCTTAAGTTTTTTTGCTGAAAGATTATTTAGCCGGTGCACGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTTAATTTATTTTTTTTTTTCTGCGTCAGGAGGTT‑GTAAATTTTGTTTT‑TGGTTTCCCTTTTTGCCAGCCTGTTAGCTTTTGGGTGTTTTTCCTTTTTTTCGATCTGCCCTTCCGCTTTTTTTTTTTCGTAggatt                                                                                                           >  2:347021/1‑235 (MQ=255)
                                                                                                                                 gACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCCTGTGCGCCATACTGTTACCTTTTGGCTGTTTTTCCATTTTTGCGATTTGCGCTTCAGCTTTTTCTTTATTGAAGGAAt                                                                                                           >  1:162436/1‑238 (MQ=255)
                                                                                                                                 gACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGAAGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTTCTTTTTCTGTTCCGGCGTCAGGATGTT‑TTAAATTTTGTTCT‑GGGTTTCCCTTTGGGCCAACATGTTAAGTTTTGGCTGTTGTTCCATTTTTTTGGTCTTCGTTTTCGCtttttattttttcaaggtta                                                                                                           >  1:1320472/1‑222 (MQ=255)
                                                                                                                                 gACCG‑GCGGTCTTAAATTTTTTTGCTGAAAGATTATTTAGCAGTTGCAGGGATTTTTCCTTTTGCCCCTGGACGTTTTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGTTTTTTTTGTTTCGGCGGCTGGAGGTT‑GTTAATTTTGTTTT‑GGGTTTCCTTTTTCGCCAGCATGTTAGCTTTGTGCTGTTTTTCCCTTTTTTCGGTTTGCGCTTCACCTTTTCCttttttgtagggttc                                                                                                          >  2:302938/1‑227 (MQ=255)
                                                                                                                                    cg‑gcggTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCt                                                                                                       <  1:1122507/239‑1 (MQ=255)
                                                                                                                                           tCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                  <  1:136085/238‑1 (MQ=255)
                                                                                                                                            ctttatttttttGGTGTAAAGTTTATTCAGCAGTTGTAGGCATTTTTCCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCTGCAGGATGTT‑GTAAATTTTGTTCG‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                <  2:1378050/233‑1 (MQ=255)
                                                                                                                                            cTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCGG‑ACGATTCTCAAAATTA‑T‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                <  1:664586/239‑1 (MQ=255)
                                                                                                                                            cTTAAGTTTTTTGGCTTAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                <  1:932770/239‑1 (MQ=255)
                                                                                                                                            cTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTTTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                <  1:582178/239‑1 (MQ=255)
                                                                                                                                            cTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                <  1:146550/239‑1 (MQ=255)
                                                                                                                                            cTTAAGGTTTTTGGCTGAAAGGTTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                <  1:1085637/239‑1 (MQ=255)
                                                                                                                                                 gTTTTTTTGTTTAAAGATTATTAAGCGGTTGCAGGCATTTTACCTTTTGCCGCTGGCCGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTTTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTc                                                                                           <  2:1124079/239‑1 (MQ=255)
                                                                                                                                                     tttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGCTGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGGCagga                                                                                       >  2:535468/1‑236 (MQ=255)
                                                                                                                                                     tttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTTTTCCATTTTTGCGGTCTGCGGTTCAGCTTTTACTTTATTGAAGGTATCGTTGCCAaagattcatagc                                                                                       >  2:739890/1‑232 (MQ=255)
                                                                                                                                                     tttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTG‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAGGGGATCCCTGGCAATGATGTccg                                                                                         >  1:1378050/1‑235 (MQ=255)
                                                                                                                                                            gAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                >  1:317871/1‑223 (MQ=255)
                                                                                                                                                            gAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTAATGCATTgtgc                                                                                >  1:220052/1‑239 (MQ=255)
                                                                                                                                                            gAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTGCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                <  2:317871/223‑1 (MQ=255)
                                                                                                                                                                   tattCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGACTTCATGCATTGCGCGGGGt                                                                           >  2:1064551/1‑237 (MQ=255)
                                                                                                                                                                    attCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTAGGCTTTTACTTTATCGAAGGCATCGCTGGCAATGAGGTCATCAATTGCGCGGCGttc                                                                         >  2:605415/1‑238 (MQ=255)
                                                                                                                                                                     ttCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                >  1:1210286/1‑214 (MQ=255)
                                                                                                                                                                     ttCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                <  2:1210286/214‑1 (MQ=255)
                                                                                                                                                                           agTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCATTGATGTCATTCATTTCGCGGGGTTTTTCCAgcg                                                                 >  2:1048620/1‑239 (MQ=255)
                                                                                                                                                                           agTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcg                                                                 >  2:311373/1‑239 (MQ=255)
                                                                                                                                                                              tGCAGGCATTTTACCTTTTGCCGCTTGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg                                                              <  1:50946/239‑1 (MQ=255)
                                                                                                                                                                              tGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg                                                              <  1:344191/239‑1 (MQ=255)
                                                                                                                                                                               gCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGtt                                                                                                                                                                                                     >  2:1360695/1‑104 (MQ=255)
                                                                                                                                                                                    cATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGt                                                         <  1:125595/238‑1 (MQ=255)
                                                                                                                                                                                       tttACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg                                                              >  1:1100620/1‑230 (MQ=255)
                                                                                                                                                                                       tttACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCa                                                     >  1:1371900/1‑239 (MQ=255)
                                                                                                                                                                                       tttACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCa                                                     >  1:789797/1‑239 (MQ=255)
                                                                                                                                                                                       tttACCTTTTGCCGATGGACGTTCTGGCGGTACG‑TTCTCAAAATTA‑G‑C‑ATTGAATTGGTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTTCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCa                                                     <  1:888338/239‑1 (MQ=255)
                                                                                                                                                                                             ttttGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGt                                               <  1:1006902/239‑1 (MQ=255)
                                                                                                                                                                                              tttGCCGCTGGACTTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCTGGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGt                                               <  1:141695/239‑1 (MQ=255)
                                                                                                                                                                                                 tcccttGGTCGTTCTGTCAG‑ACTCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACg                                           <  2:1240299/234‑1 (MQ=255)
                                                                                                                                                                                                     cTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTg                                        >  1:141157/1‑238 (MQ=255)
                                                                                                                                                                                                      tGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCt                                         <  1:527137/236‑1 (MQ=255)
                                                                                                                                                                                                             tCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTGAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                >  2:570097/1‑174 (MQ=255)
                                                                                                                                                                                                             tCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACCCTGGCCTTTCAt                               >  2:445967/1‑239 (MQ=255)
                                                                                                                                                                                                                gTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCCCGGCGTTCTTCCAGCGGCGGCCCTTTCTTCTGGTCACGCTGGCCTTTccggct                            >  2:527137/1‑234 (MQ=255)
                                                                                                                                                                                                                       cGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTcg                        <  1:1066807/237‑1 (MQ=255)
                                                                                                                                                                                                                         cTTTTCAAAATTT‑G‑C‑TTTTAATTTGTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGa                    <  2:1306381/239‑1 (MQ=255)
                                                                                                                                                                                                                          ttCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGCCGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGa                    >  1:631651/1‑238 (MQ=255)
                                                                                                                                                                                                                           tctcAAATTTT‑G‑C‑ATTAAATTGTTTTTTCTGTTCCGGCGTCAGGATGTT‑GGAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATc                  <  2:1155205/239‑1 (MQ=255)
                                                                                                                                                                                                                            ctcAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg                                                              >  1:327651/1‑194 (MQ=255)
                                                                                                                                                                                                                            ctcAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGCTGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGa                    >  1:236752/1‑236 (MQ=255)
                                                                                                                                                                                                                            ctcAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCCCGGa                    >  1:1306381/1‑236 (MQ=255)
                                                                                                                                                                                                                                    tA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCtt                                                                                                                                   <  2:1116975/117‑1 (MQ=255)
                                                                                                                                                                                                                                    tA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCtt                                                                                                                                   >  1:1116975/1‑117 (MQ=255)
                                                                                                                                                                                                                                    tA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg                                                              >  2:166811/1‑186 (MQ=255)
                                                                                                                                                                                                                                    tA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg                                                              <  1:166811/186‑1 (MQ=255)
                                                                                                                                                                                                                                       g‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGTTCTGCTGTTTCTg       >  1:853415/1‑239 (MQ=255)
                                                                                                                                                                                                                                        agtaTTAAATTGCTTTTTCCTGTCCGGGGTTAGGATTTT‑TTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCATCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGTAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCt        <  2:789797/236‑1 (MQ=255)
                                                                                                                                                                                                                                         c‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTg                                                                                                                                        <  2:1027456/109‑1 (MQ=255)
                                                                                                                                                                                                                                         c‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTg                                                                                                                                        >  1:1027456/1‑109 (MQ=255)
                                                                                                                                                                                                                                             tAAATTGCTTTTTCCGTTCCGGCGTCAGGATTGT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGcgcg   <  1:691631/239‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           
ACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAG‑ACGCTTCTCAAAATTA‑G‑C‑ATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTT‑GTAAATTTTGTTCT‑GGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGT  >  NC_000913/1824980‑1825450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A