Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,098,141 T→C 14.0% E54G (GAG→GGG)  wzzB ← regulator of length of O‑antigen component of lipopolysaccharide chains

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,098,1410TC14.0% 248.8 / 8.7 101E54G (GAG→GGG) wzzBregulator of length of O‑antigen component of lipopolysaccharide chains
Reads supporting (aligned to +/- strand):  ref base T (46/40);  new base C (2/13);  total (48/53)
Fisher's exact test for biased strand distribution p-value = 4.58e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 102 total aligned reads displayed.

GTCAATGGTAATTGCTGGTTCTTAACAGAAGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAA  >  NC_000913/2097904‑2098373
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            
gTCAATGGTAATTGCTGGTTCTTAACAGAAGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGA‑CAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTtct                                                                                                                                                                                                                                              >  2:418786/1‑237 (MQ=255)
        tAATTGCTGGTTCTTAACAGAAGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTa                                                                                                                                                                                                                                    <  1:687372/239‑1 (MQ=255)
          aTTTCTGGTTTTTAACAGGAGGTTTGATGGTAAAGTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAAGGCAGAGAAGGCAGAACTGAAGCGAACAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACt                                                                                                                                                                                                                                  <  2:899044/239‑1 (MQ=255)
           ttGCTGGTTCTTAACAGAAGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCACACGCCGGGCTGATTGATAATTGCTGTTA‑ACGACCATTTCC‑CCTTCGCTACt                                                                                                                                                                                                                                  >  2:370540/1‑238 (MQ=255)
             gttttaaaggacaggAAGGTTCGGTGGTAAGTTTT‑TCTCGGTCTACCTGATTTTCCAGCGTTTCTGCTAATGCAGAGCAGGCCGAACGGAAGCGACCAATTAAC‑GTCTCCCGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGcc                                                                                                                                                                                                                               <  2:1003453/225‑1 (MQ=255)
             gCTGTTTTTTAACAGAAGTTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGcc                                                                                                                                                                                                                               <  2:765983/239‑1 (MQ=255)
              cTGGTTCTTAACAGAAGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGA‑CAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCaa                                                                                                                                                                                                                             <  1:418786/239‑1 (MQ=255)
               tgttttttAACAGAAGGTTCGTTGGTAAGTTTT‑TCCCGTTCTTCCCGATTTCCCCGCTGTTCCGCAAAGGCAAGGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCGGCAAATCCGATTCTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCaa                                                                                                                                                                                                                             <  2:191471/233‑1 (MQ=255)
               tGGTTTTTTAAAGAAGGTTCGATGGTAAGTTTG‑TCGTGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGGCCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCaa                                                                                                                                                                                                                             <  2:447597/239‑1 (MQ=255)
                ggTTCTTAACAGAAGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGGCCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTTCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTCCTGCCaaa                                                                                                                                                                                                                            >  1:448944/1‑239 (MQ=255)
                 ggtttttaCAAAAGCTTCGGGGGTAAATTTTCTCTCTTCTTTCCTG‑TTCGCCCGCTTTTCTGCCAATTAAGGGAAGGCAGTACTGAAGCGACCAAGTAGC‑GTCTCCTGTAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCCCGTCGGGCTGAGTGATTATTGCTTTTG‑ACGTCCATTTCC‑CCTTCGCTACTGCCAAat                                                                                                                                                                                                                           <  2:1129615/232‑1 (MQ=255)
                 gTTCTTAACAGAAGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGTTTTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCaaaa                                                                                                                                                                                                                           >  2:350907/1‑238 (MQ=255)
                 gTTCTTAACAGAAGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCGGAGGGTGAATGACTTTTG‑ACGTCCATTTTC‑CCTTCgtacctcgcaaaa                                                                                                                                                                                                                           >  1:658736/1‑227 (MQ=255)
                      tAACAGAAGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCa                                                                                                                                                                                                                      <  1:977573/239‑1 (MQ=255)
                      tAACAGAAGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCa                                                                                                                                                                                                                      <  1:368635/239‑1 (MQ=255)
                           gAAGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCa                                                                                                                                                                                                                      <  2:336058/234‑1 (MQ=255)
                           gAAGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCa                                                                                                                                                                                                                      >  1:336058/1‑234 (MQ=255)
                            aaGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGATGGATAATTGCTGTTT‑ACGTCCATTTCT‑CCTTCCCTACTGCCAAATATCCAATAGcc                                                                                                                                                                                                                >  1:1349146/1‑239 (MQ=255)
                            aaGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAAAAGcc                                                                                                                                                                                                                >  1:122754/1‑239 (MQ=255)
                             aGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTGCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGc                                                                                                                                                                                                                 <  1:1018913/237‑1 (MQ=255)
                             aGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCGCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCa                                                                                                                                                                                                               <  1:95661/239‑1 (MQ=255)
                             aGGTTCGATGGTAAGTTTT‑TCCCGTTCTTCCTGATTAACCAGCGTTTCTTCAAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGc                                                                                                                                                                                                                 <  1:580993/237‑1 (MQ=255)
                                  ctgtGGTAAGTTGT‑TCCCGTTCTTTCCGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAATCGCCCAATTAAC‑GTCTCCTGCAAATCCGATACGTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGGCGGGCTGAGTGATAATTGCTGTTG‑AAGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCa                                                                                                                                                                                                          <  1:317904/236‑1 (MQ=255)
                                      ggTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCaa                                                                                                                                                                                                                     >  1:1003453/1‑224 (MQ=255)
                                               t‑tCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCCCTCCTGCCAAATATCCAAAAGCCAGGGCAAAAA‑CCACaaaaa                                                                                                                                                                                            >  2:1309222/1‑236 (MQ=255)
                                               t‑tCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTACCCCACGTCGGGCTGAGTGATAATTGCCGTTG‑ACGTCCATTTCT‑CCTTCGCTACTCCCAAATATACAATAACCAGGGCAATAC‑CCACAA‑AGa                                                                                                                                                                                           >  2:607229/1‑239 (MQ=255)
                                               t‑tCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAAATAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGa                                                                                                                                                                                           <  1:1056947/239‑1 (MQ=255)
                                                         ttccttgtttcccAGCTTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAatga                                                                                                                                                                                  <  2:1349146/228‑1 (MQ=255)
                                                         ttcCTGTTTACCCTGCCTTTCCGCTAATGAAGAGAAGGCAGAACTGAAGCGACCAATAAAC‑GTCCCTTGCAAATCCGATGATTTCGGTGAAGCCTGACCATAGCTAACATTCATGGCATTGTTATAGCCAGCAATTTTCCCCACTTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTCCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAatga                                                                                                                                                                                  <  2:765427/239‑1 (MQ=255)
                                                              gATTAGCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACCGAAGCGAACAATTAAC‑GTCTCCTGCAAATCCGATACTTACGGTACAGCCTGACCATAGATAACATGCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTT‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGAtt                                                                                                                                                                             <  1:1321631/239‑1 (MQ=255)
                                                                         gTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTgcc                                                                                                                                                                  >  1:122768/1‑239 (MQ=255)
                                                                           ttCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATAAAGATTGTAATCTTgccac                                                                                                                                                                >  2:1123167/1‑239 (MQ=255)
                                                                             cttcTAAGGCAGAGAAGCCAAAACTAACGGGCCCAATTAACTGTC‑CCTGCAAACCCGATTCTTTGGGGCCAGCTTGACCATAGATAACATTCTTGGAATTGTTATGGCCAGCAATTTCCCCCCCGGCGGGTGGAGTGATAATTGTGGTGG‑ACGTCCATTTCT‑CCTTCGCTACTGCAAAATATCCAATGGCCAGGGCAATAG‑CCAAAA‑TGAGGGAAATGATGATTGTCATCTTgccacgc                                                                                                                                                              <  2:1117364/236‑1 (MQ=255)
                                                                             cTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTATCGATGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAAATTGCCCCACGTCGGGCTGAGCGATAATTGCTGTTG‑ACGTCAATTTCT‑CCTACGGTACTGCCAACAATCCAATATCCAGGGCCAAAA‑CCACCA‑TGACGGAAAAGATGATTGGCACATTcccacg                                                                                                                                                               >  2:458345/1‑238 (MQ=255)
                                                                                      cAGAGAGGGCAGCACTGAAGCAACCAATTACC‑TTCCCCTGCAAATCCGATTCTTGCGGTGCAGCTTGCCCAAAGATAACATGCATGGTATTGTTAGAGCCAGCATTTTGCCCCCCGCGGGGCTGAGTGATAATTGTTGTTG‑ACGCCCTTTTCT‑CCTTCGCTACTGCCAAATCCCCAATACCCAGGCCAATAG‑CCAAAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGc                                                                                                                                                     <  2:27435/239‑1 (MQ=255)
                                                                                          gaAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATCACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGTCTTAGTGATGATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCCGGGCAATAG‑CCACAC‑TCACGGCAACCACTTTTTTTATCTTGCCACCCCCCAACTACCCTa                                                                                                                                                 >  2:614701/1‑239 (MQ=255)
                                                                                          gaAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATACATATTGTCATCTTGCAACGCCACAAcagaacaa                                                                                                                                                 >  2:1300800/1‑232 (MQ=255)
                                                                                               cAGAACTGAAGCGAACAATTAAC‑GTCTCCTGCAAACCCGATACTTTCGGTGCAGCCTGACCATGAATACAATTCATGGAATTGTTAGAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTATTGCCAAATATCAAATAGCCAGGGAAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTaa                                                                                                                                             <  1:1309222/238‑1 (MQ=255)
                                                                                                       aaGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGATATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTA‑ACGTCAATTTCT‑CCTTCGCTACTGCCAAATAGCCAATATCCAGGGCACTAG‑CCCCAC‑GGTCGGTAATAATGACTGCCATCTTGCAACCCCACAACTGAACTAGTaaatcacacaa                                                                                                                                    >  1:860369/1‑234 (MQ=255)
                                                                                                        accGACAATTTAAT‑GTTCCCTTCAAATCCGTTACTTTGGGGGCAGCCTGACCAAAGAAAACATTCATGGCTTTTGTAAAGCCAGCATTTTTCCCCACGTGGGGCTGAGTGAAAATTGCTGTTG‑AGGTCCATTTCT‑CCTTCGCTACTGCAAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTaaatcaatcaaa                                                                                                                                   <  2:216805/237‑1 (MQ=255)
                                                                                                            aCCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTaaatcaatcaaatcaa                                                                                                                               >  1:1225556/1‑239 (MQ=255)
                                                                                                            aCCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGCCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCCCAA‑TGACGAAAATGATGATTGTCATCTTCCAACCCCAAAACCGCAATAGAaaaaaaaccaaataaa                                                                                                                               >  2:418802/1‑239 (MQ=255)
                                                                                                             ccAATTAAC‑TTTCCCTGCAAAGCCGATCCTTGCGGGGCAGCCTGCCCAAAGATAACATTCATGGCATTGTTATGGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑CCGTCCATTTCC‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTaaatcaatcaaatcaat                                                                                                                              <  1:1286249/239‑1 (MQ=255)
                                                                                                             ccAATTAAC‑GTCTCCTGCAATTCCAATACTTTGGGTGCAGCCTGCACATAGATAACATTCATGTGTTTTTTATAGCAAGAAATTTCCCCCACGTGGGGGTGAGTGAAAATTGCTTTTG‑ACGTCCATTTCC‑CCTTCGCTACTGCCAAATACCCAAACGCCAGGGCAATAG‑CCAAAA‑TGACGAAAATGATGATTTTCATCTTGCCACGCCACAACTGCACTAGTaaatcaatcaaatcaat                                                                                                                              <  1:1338818/239‑1 (MQ=255)
                                                                                                                    aacgctcccTGAAAATCCGATACTTTCGGTGCAGCCTGACCAAAGATAACATTCAGGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATGGCTGTTG‑ACGTCCATTTCT‑CTTTCGCTACTGCAAAATATCAAATAGCCAGGGCAATAG‑CCAAAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTc                                                                                                                        <  1:212436/232‑1 (MQ=255)
                                                                                                                          tcCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAT‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCCAACCTGCACTAGTAAATCAATCAAATCAATCCGTTCCgggcc                                                                                                                  >  2:212436/1‑237 (MQ=255)
                                                                                                                          tcCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAC‑TGACGGAAATGATGATTGTCATCTTGCCTCGCCACAACTGCAATAGTAAATCAATCAAAACAAACTGTTCCGGGTc                                                                                                                  >  2:1100381/1‑239 (MQ=255)
                                                                                                                          tcCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTc                                                                                                                  >  1:398025/1‑239 (MQ=255)
                                                                                                                           ccTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAAACAAATCAATCGTTTCCGGGTCa                                                                                                                 >  2:600999/1‑239 (MQ=255)
                                                                                                                                 aattgtatatttgaggTGCAGCGTGACCATAGATAGAATTAATGCCTTTGTTATAGCCAGTAATTTGCCCCACGTGGGGGTGGGTGAATATTGCTGTTG‑CAGTCCATTTCT‑CCTCCGCCACTGCAAAATTGCCAAGAGCCAGGGCAATAG‑CAACAA‑TGACGGAAATGATGATTGTCATCTGGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGttt                                                                                                           <  2:209570/225‑1 (MQ=255)
                                                                                                                                       aTACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAACTCAATCTTTTCCGGGCCATGGTTTTCCCCa                                                                                                     >  2:847837/1‑239 (MQ=255)
                                                                                                                                         ccTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTTTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGGGATAATTGCTGTGG‑ACGTCCATTCCC‑CCTCCGCTACTGCAAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGa                                                                                                   <  1:1300800/238‑1 (MQ=255)
                                                                                                                                                           ccATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTat                                                                                 >  1:1183116/1‑238 (MQ=255)
                                                                                                                                                            gagaaatatcttcaaTGGCATTGTTAAAGCCGGCATTTTGCCCCCGGTCGGGCTGAGTAAAAATTGCTGTTG‑ACGCCAATTTCT‑CCTTGCCTACTACAAAATTTCCAATCCCCAGGGAAATAG‑CCACAA‑GGACGGAAATGATGATTGTCATCTTCCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTAct                                                                                <  1:1172316/225‑1 (MQ=255)
                                                                                                                                                            aaTAGATAACATTCAGGGCATTTTTATAGCCAGCAATTGGCCCCACGTCGGGCTGAGTGATAATTGCTTGTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATTTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTAct                                                                                <  1:1116152/238‑1 (MQ=255)
                                                                                                                                                             atagtaacattttgtGGCATTTTTAAACCCAGCAATTGCCCCCAGGTCGGGCGGAATGATAATTGCTTTTA‑CCGTCCATTTCC‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGAAAATAG‑CCACAA‑TGACGAAAATGATGATTGTCATTTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTActc                                                                               <  1:563882/225‑1 (MQ=255)
                                                                                                                                                              taaaTAACATTCGTGGTATTTTTATAGCCAGCAATTTCCCCCCCGTCGGGCTGAGTGATAATTGCTTGTG‑GCGTCAATTTCT‑CCTTCGCTACTGCCAAATACCCAATAGCCAGGGCAATAG‑CCAAAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTActct                                                                              <  1:600999/236‑1 (MQ=255)
                                                                                                                                                                gATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCCGAAACATTATTATTTTCTACtctc                                                                             >  1:701267/1‑238 (MQ=255)
                                                                                                                                                                aaTAACATTCATGGCATTTTTATACCCAGCAATTTCCCCCGCGGGGGGCTGAGTAATAATTGCTGTTG‑ACGTCCTTTTCT‑CCTCCGCTACTGCAAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTActct                                                                              <  1:849320/236‑1 (MQ=255)
                                                                                                                                                                   aactttaagggaaTTGTTAAAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATACCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCtcatc‑a                                                                        <  1:847837/227‑1 (MQ=255)
                                                                                                                                                                   aaCCTTAATGGATTTGTTAAAGCAAGAAATTTGCCCCACGCCGGGCTGAGTAATAATTGCTGTTG‑ACGCCCATTCCT‑CCTTCGCTACTGCCAAATACCAAATGGCCTGGGAAATAG‑CCACAA‑TGACGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCtcatc‑a                                                                        <  1:350907/239‑1 (MQ=255)
                                                                                                                                                                   aaCATTCAGGGCATTGTTATACCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGTTGTAG‑ACGCCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGCCAATCG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCtcatc‑a                                                                        <  1:74511/239‑1 (MQ=255)
                                                                                                                                                                     cATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGCAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGGTTTGCCCAGAAACATTATTATTTTTTACTCTCATC‑Att                                                                      >  1:283542/1‑239 (MQ=255)
                                                                                                                                                                     cATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ttt                                                                      >  1:801281/1‑239 (MQ=255)
                                                                                                                                                                      aTTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑Att                                                                      >  1:1120927/1‑238 (MQ=255)
                                                                                                                                                                      aTTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGGAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCAAGAAACattatta                                                                                         >  1:669147/1‑220 (MQ=255)
                                                                                                                                                                         cATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTAccc                                                                  >  1:990071/1‑239 (MQ=255)
                                                                                                                                                                          aTGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAC‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCCATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTTATTTATACTCTCATC‑ATTACCCt                                                                 >  2:783301/1‑239 (MQ=255)
                                                                                                                                                                          aTGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGCTTGGCATCTTGCCACGCCACAACTGCACTAGTAAATCACTCAAATCAATCTGTTCCGGGCCATGGTTTTGCCCAGAAACATAATTATTTTCAACTCTCACA‑ATAAccca                                                                 >  2:155611/1‑238 (MQ=255)
                                                                                                                                                                                ttGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGCAACATTATTATTTTCTACTCCCATC‑ATTACCCTAAATg                                                            >  2:1036965/1‑238 (MQ=255)
                                                                                                                                                                                 tGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGCCGGAAATGATGATTGTCATCTTGCCCCGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGACCTGGTTTTGCCCAGAAACATTATTATTTTCTACTCTACTC‑CTTACCACAACttcc                                                          >  2:468396/1‑236 (MQ=255)
                                                                                                                                                                                     ataGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCCGGGCAATCG‑CCACAA‑TGACGGGAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATAAATCCTTTCCGGGACAGGGTTTTGCCCCGAAACATTATTATTTTCTATTCCCATC‑ATTACCCTAAATGACGGaa                                                      >  2:176783/1‑239 (MQ=255)
                                                                                                                                                                                          catcagtttCCCCCCCGTGGGGCTGGGTGATATGTGCTGGTG‑ACCACATTTTCC‑ACTTCACTCCGGCAAAATATCCATGGGCCAGGGAAATAA‑CCAAAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTc                                                 <  2:205977/233‑1 (MQ=255)
                                                                                                                                                                                           aGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGCGTCATGGTTTTGCCCAGaaa                                                                                                 >  1:618106/1‑191 (MQ=255)
                                                                                                                                                                                             cAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACt                                                                                                                                                                                                                                  >  1:386437/1‑62 (MQ=255)
                                                                                                                                                                                             cAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACt                                                                                                                                                                                                                                  <  2:386437/62‑1 (MQ=255)
                                                                                                                                                                                             cAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAAACTGTTCCGGGTCATGGTTTTGCCCCGAAACATTATTATTTTCTACTCTCATC‑ATTACCCCAAATGACGGAAATTTCCaa                                              >  2:79572/1‑239 (MQ=255)
                                                                                                                                                                                             cAATTTCCCCCACGTCGGGCTGAGTGATAATTGCTGTTC‑ACGTCCATTTTT‑CCTTCGCTACTGCCAAAGATCCAATAGCCTTGGCAATAC‑CCACAA‑TGACGCTAATGACGATTACCATCTTCCCCCTCCACAACTAACCTAGTAAATCAATCAAATCACACTGTTCTTGGTCATGATTTTACCCAGAAACATTATAATTTTCCACTCACAAC‑ATAACCCAAACAAAAGGAAAATTCCaa                                              >  2:584318/1‑239 (MQ=255)
                                                                                                                                                                                                    ccccACGGCGGGTTTATTTATAAGTGCTTTTG‑ACGCCCATTCCC‑CCTTGCCTACGCCAAAATAGCCAATGCCCAGGACAAGAC‑CTACAA‑TGGCGGAAATGATGATTGTCTTCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCa                                       <  1:617034/239‑1 (MQ=255)
                                                                                                                                                                                                    ccccACGGCGGGTGGATGGATAATGGCTGTTG‑ACGTCCTTTTCC‑CCTTCGTATCTGACAAATTTCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGCCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCACAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCa                                       <  1:458345/239‑1 (MQ=255)
                                                                                                                                                                                                     cccccgcggagCTGAGAGATAATGGCTGTTG‑CCGTCCATTTCT‑CCTTCGCTACTGCTAAATATCCAATTACCGGGGCAATAG‑CCACAA‑TTACGGAAATTATGATTGGCATCTTCCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCa                                       <  1:1036965/228‑1 (MQ=255)
                                                                                                                                                                                                     ccccccgtgggggtgggttaaaTTGCTGGTGCACTCCCTTTTCC‑CCTTGGCTACTGCAAATTACCCAACACCAGGGGCAATAG‑CCAAAA‑GGAGGAAAATGATGATTTGCATTTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCa                                       <  2:780001/219‑1 (MQ=255)
                                                                                                                                                                                                         cgccGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCTCTACTGCCAAATATCCAATAGCCAGGGCAATAA‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAGTCAAACAAATCAATCTGTTCCGGGTAATGGTTTTGCCCCGAAACATTATAATTTTC‑AATTTCAACAATAACCCTAACTGACGAAAATTTCCAAGTcccaatattt                                  >  2:240621/4‑231 (MQ=255)
                                                                                                                                                                                                                   aGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTACCGATaa                         >  2:579564/1‑238 (MQ=255)
                                                                                                                                                                                                                    gttaTAAGTGGTGTTT‑GCGTCATTTTCT‑CCTTCGATATTCACAATATTCCATGCCCCGGGGCAATAG‑CCACAAGTGA‑GGAAAATATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGTTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATc                       <  2:658736/236‑1 (MQ=255)
                                                                                                                                                                                                                    gggATAATTGCTGTTG‑TCGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATCGTCCGGGCAATAG‑CCACAA‑TGACGGATATTATGATTTTCATCTTGCCACGCCACTTCTGCACTCGTAACTTAATCCAATCAAACTGTTCCGGGTCATGTTTTTGCCCACAAACATTATTCTTTTCTACTCCCATC‑ATTACTCTAACTGTCGGAAATTTTCAAGTccacctctttttaccaaaaa                        >  2:626584/3‑220 (MQ=255)
                                                                                                                                                                                                                         aaTTGCTGTTG‑ACGTCCTTTTCC‑CCTTCGCTACTGCCAAATATCCAAGAGCCAGGGCAAGAG‑CCACAA‑TGACGGAAATGATGATTGTCTTCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGt                  <  1:1081725/239‑1 (MQ=255)
                                                                                                                                                                                                                           ttgttgTGG‑ACGTCTATTTCT‑CCTTCGCTACTGCCAAATTCCCAATCGCCGGGGCAATCG‑CCAAAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGa                <  1:176783/239‑1 (MQ=255)
                                                                                                                                                                                                                            ttttttttgccttcttttttT‑CCTTCCCTAC‑GGCAAAAATCCAAAAACCCGGGCAAAAGCCCAAAA‑TGGCGAAAATGATTATTTCCATCTGGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGa                <  2:122754/223‑1 (MQ=255)
                                                                                                                                                                                                                            tgcttgtG‑ACGTCCTTTTCC‑CCTTCGCTATGGCCAAATTCCCAAGGCCCAGGACAATAG‑CCACAA‑TAGCGAAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGaa               <  1:370540/233‑1 (MQ=255)
                                                                                                                                                                                                                            tGCTTTTG‑CCGTCCATTTCC‑CCTTCGCTCCTGCAAAATCTCCAATAGCCAGGGAAATAG‑CAACAA‑TGACGGAAAGGATTATTTTCAACTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGaa               <  1:517195/239‑1 (MQ=255)
                                                                                                                                                                                                                            tGCTTTTG‑ACGTCCATTTCT‑CCGTCGCTACTGCCAAATATCCAATAGCCAGGGAAATAG‑CCAAAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCATAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGaa               <  1:1123167/239‑1 (MQ=255)
                                                                                                                                                                                                                            gtcTGTGG‑ACTTCCTTTTCT‑CCTCCGCTCCTGAAAAATATCCAAAGGCGAGGGAAAGAG‑CCACAA‑TGACGTAAAGGAGGATTGTCATCTTCCCACGCCACAACTGCACTAGTAAAGCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAAGTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGaa               <  2:701267/237‑1 (MQ=255)
                                                                                                                                                                                                                             gggtgtgaacgggcgtttCC‑CCTTCGCTACTCAAAAATACCCAATAGCCAGGCCAATAG‑CCAAAA‑TGACGGAAATGATGATTGCCATCTTGCCACGCCACAACTGCACTAGTAAAGCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGaa               <  1:155611/224‑1 (MQ=255)
                                                                                                                                                                                                                             gCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGGAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATAATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCAAAGTTAACCGAAAAACCTCGTGaaa              >  2:517195/1‑238 (MQ=255)
                                                                                                                                                                                                                             gCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGCCGGAAATTTCCAAGTCGCCATAGTTTAACGATAACCCCCGGGaaa              >  2:1186603/1‑238 (MQ=255)
                                                                                                                                                                                                                             gCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGCGAAt              >  1:1044479/1‑239 (MQ=255)
                                                                                                                                                                                                                              tgttttggccgccctttCTCCCTTCCATACTGCAAAATTTCAAAAAGCCAGGGAAATAC‑CAAAAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGaa               <  2:655171/225‑1 (MQ=255)
                                                                                                                                                                                                                                 ttG‑CCGTCAATTTCT‑CCGTCGCTCCTGCCAAATATCCAAGAGCCAGGGCAATAG‑CCAAAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCta          <  1:1186603/239‑1 (MQ=255)
                                                                                                                                                                                                                                          cATTTCT‑CCTTCGCTACTGCCAAATATCCAATATCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCACGTCCTCACAGTTTAGCGATAATCCTCGTGAATGCTATAGGTTaa  >  2:231694/1‑239 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            
GTCAATGGTAATTGCTGGTTCTTAACAGAAGGTTCGATGGTAAGTTTT‑TCTCGTTCTTCCTGATTATCCAGCGTTTCTGCTAATGCAGAGAAGGCAGAACTGAAGCGACCAATTAAC‑GTCTCCTGCAAATCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTG‑ACGTCCATTTCT‑CCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAG‑CCACAA‑TGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATC‑ATTACCCTAACTGACGGAAATTTCCAAGTCCGCATAGTTTAGCGATAATCCTCGTGAATGCTATAGGATAA  >  NC_000913/2097904‑2098373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A