Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,140,197 T→G 11.5% H675P (CAC→CCC)  frwA ← putative PTS enzyme: Hpr, enzyme I and II components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,140,1970TG11.5% 350.1 / 4.0 143H675P (CAC→CCC) frwAputative PTS enzyme: Hpr, enzyme I and II components
Reads supporting (aligned to +/- strand):  ref base T (41/75);  new base G (23/3);  total (65/78)
Fisher's exact test for biased strand distribution p-value = 1.18e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

TCTGATTTGCTGTGTGGAATGGCAAAACTAAAGCCCAGACCGGTAGAGAAAA‑CGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGT  >  NC_000913/4139960‑4140425
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                     
gcTGATTTGCTGTGTGGAATGGCAAAACTAAAGCCCAGCCCGGTAGAAAAAC‑CGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGgt                                                                                                                                                                                                                                       <  1:1027378/237‑1 (MQ=255)
    ttttcgggtgtgGAAGGTCAAACCAAAAGCCCCACCCGGGA‑AGAAAACCGCCCCCGCGCCCCCCAAGGTCGGCTCCCGTTTTCCGCGGAAGGCGGCGGGGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCt                                                                                                                                                                                                                                  <  2:1332813/232‑1 (MQ=255)
            tgtgGAATGGCAAAACTAAAGCCCAGACCGGTAGAGAAAA‑CGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGCCTTGTTGGGTCATGCGGAATTGCGCCCGCAGGGGTTCTACTTCCa                                                                                                                                                                                                                           >  1:1018763/1‑238 (MQ=255)
              tggaatggaaAAACTAAACCCCAGGCGGGTAGAGAAAA‑GGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCg                                                                                                                                                                                                                        <  2:125289/239‑1 (MQ=255)
              tgGAATGGCAAAACTAAAGCCCAGACCGGTAGAGAAAA‑CGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACCCGGCGGTGGACAGCGGGGCGTCTTGTTGGTGCATGAGGGATTGGGCCCGCAGGGGGTCTACTTTCcgcg                                                                                                                                                                                                                        >  2:1237031/1‑239 (MQ=255)
                   tGGCAAAACTAAAGCCCAGACCGGTAGAGAAAA‑CGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGAAGGGGTTCTCCTTCCAGCGAAGt                                                                                                                                                                                                                    >  1:1093372/1‑238 (MQ=255)
                           ttAAACCCCCGCCCGGTAGAAAAAG‑CGCCCCCGCGCCCCCAAAAGCCGGTTCCCAGTTTGCGGGGATAGGGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTTTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGc                                                                                                                                                                                                           <  2:918253/238‑1 (MQ=255)
                           ccaaaGCCAGGCCCGGGAAAGAAAC‑CGGCCCCGCTACCCCACAGGCCGGCTTCCAGTTTACGCGGACAGCGGCATCGGCCCGTCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGc                                                                                                                                                                                                           <  1:739044/237‑1 (MQ=255)
                           cTAAAGCCCAGCCCGGTAGAGAAAA‑CGGCCTCGCGCGCCCAAAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGc                                                                                                                                                                                                           <  1:1225546/239‑1 (MQ=255)
                            ataagacacggcgggaagaaAAAA‑GGCCCGCGGGGGCCCACAGGTGGTCTTCCAGTGTAGGCGGATTGCGGCAGGGGCCCGCCAGGAGCAGGTTATCGGTCATGCCTTTGGGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGc                                                                                                                                                                                                           <  2:676065/222‑1 (MQ=255)
                             aaaGCCCAGCCGGTTAGAGAAAC‑CGTTGTCGCGCCCCCCCAGGGTGTCTTCGAGTTTACGCGGATGGCGGCAGGGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCa                                                                                                                                                                                                         <  2:488162/239‑1 (MQ=255)
                             aaaGCCCAGACCGGTAGAGAAAA‑CGGCCTCGGGCGCCCACAAGTGGGCTTCCAGTTTACGGGGAAAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCa                                                                                                                                                                                                         <  1:725929/239‑1 (MQ=255)
                               aGCCCAGACCGGTAGAGAAAA‑CGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCCACGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGGATTCTACTTCCACCGAAGTACCGACGGCCAtt                                                                                                                                                                                                       >  1:595733/1‑239 (MQ=255)
                                  ccAGGCGGGGAGAGAAAC‑CGGCCTCGGGCGCCCACAAGTCGGCTTCCAGTTTACGGGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGcc                                                                                                                                                                                                    <  1:531931/239‑1 (MQ=255)
                                     gACCGGTAGAGAAAA‑CGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGCGACCAGCGGTGCGTCTTGTTCGGTCATCCGGGATTGCGCCAGCCGGCGTTCTACTTCCAGCGAAGGACGGGAGGCCATTGCCTgg                                                                                                                                                                                                 >  1:778859/1‑239 (MQ=255)
                                      aCCGGTAGAGAAAA‑CGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGGGCCGGAAGGGGTTCTCATTCCAGCGAAGTACGCCCGGCCATTGCCCGGt                                                                                                                                                                                                >  1:5802/1‑239 (MQ=255)
                                                aaaa‑CGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGCTGCCCTCGGCGGTGACCAACGGTGGGGCTTGTTGGGTCATGGGGAATTTCGCCCGCAGGGGGTCTCCTTCCAGCGAGGTAAGGCCGGGCCATGCCTGGGTGGGAAAcat                                                                                                                                                                                      >  2:271028/1‑237 (MQ=255)
                                                  aa‑CGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGGGCGTCTTGTTGGGTCATGCGGAATTGCGCCCGCAGGGGTTCTACTTCCAGCGCAGTACGGCAGGCCATTGCCTgg                                                                                                                                                                                                 >  1:676065/1‑226 (MQ=255)
                                                   a‑CGGCCTCGGGCCCCCACAAGTCGGCTTCCATTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCg                                                                                                                                                                                   <  1:565365/239‑1 (MQ=255)
                                                   a‑CGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGAGGGGCTTTTTGGGGCATGCGGACTTGGTCCAGCAGGGGGTGTACTTCCAACGAAGTACGGACGGCCACTGCCCGGTTGGCGAACTTggg                                                                                                                                                                                   >  2:250356/1‑237 (MQ=255)
                                                   a‑CGCCCTCCGGCGCCCACAAGTCGGCTTCCATTTTACGCGGATCGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCg                                                                                                                                                                                   <  1:1372694/239‑1 (MQ=255)
                                                     cGGCCCCCCGCCCCAACAAGTGGGTTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCgg                                                                                                                                                                                  <  1:324982/239‑1 (MQ=255)
                                                         cTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGCATTTCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCCTTGCCTGGTTTGGCAACTTGCggaca                                                                                                                                                                               >  1:913690/1‑235 (MQ=255)
                                                            cccccccccCAAGTCGGTTTCCAGTTTACCCGGATGGGGGCTGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAc                                                                                                                                                                           <  2:1172245/234‑1 (MQ=255)
                                                              gcgcCCACAAGGCGGCTTCCATTTAACGGGGATAGCGGCAGGGGCCCGTCAGCAGCAGGTTATCGGTCATGCCTTGGAGCCCTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑gg                                                                                                                                                                        <  1:985338/239‑1 (MQ=255)
                                                                            gcttccgtttaccggcggtAGCGGCCGCCGCCCGCCAGCGGCAGGTTATCGGTCATGCCTTTGAGCCTGTCTTCGTGGCTGCGTCAGTCGCGTTCCAGTGTGATGCCCTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTgcg                                                                                                                                                           <  2:913690/220‑1 (MQ=255)
                                                                            gCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCACCGGTGCGTCTTGTTGGGGCATGCGGAATTGCGCCAGCAGGGGGTCTACTTCCCGCGAAGTACGGCAGGCCCTTGCCTGGTTGAGCAACTTCCGGCACTCCC‑GGGCATCaaacgggg                                                                                                                                                           >  2:1207751/1‑232 (MQ=255)
                                                                                cccttttaggggtatggcggcggtgccCCGCCAGCGCGAGGTTACCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATc                                                                                                                                                      <  2:124724/223‑1 (MQ=255)
                                                                                ccAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGGGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCAt                                                                                                                                                       >  1:118955/1‑238 (MQ=255)
                                                                                cacttttCCGCGGTGAGGGGCCGGGCCCCGCCAGCAGCAGGTTATGGGTCATGCCTTTGAGCACTTCTGTTTTGCTGCGCCAGGCGCTTTCCAGTGTGATGCCCTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATc                                                                                                                                                      <  2:1374512/235‑1 (MQ=255)
                                                                                 cggtttgcgcgggtgggggcgtgggcCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATcc                                                                                                                                                     <  2:1093372/216‑1 (MQ=255)
                                                                                        cgcgGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCCAGTTGCGCCATCCGCGCtt                                                                                                                                               >  1:981765/1‑238 (MQ=255)
                                                                                           ggagtgcggcagcggcCCGGCAGCAGCAGGTTATCGGTCATGACTTTGAGCCCTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCGTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTcg                                                                                                                                            <  1:339851/233‑1 (MQ=255)
                                                                                              tAGGGGCCGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTcgccg                                                                                                                                         <  1:1271338/238‑1 (MQ=255)
                                                                                               aggggcagcggcCCGCCCGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCgccgcc                                                                                                                                       <  1:465388/236‑1 (MQ=255)
                                                                                                ggggcggcggcCCGCCAGCAGCAGGTTATCGGTCATGCCTTGGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCgccgcc                                                                                                                                       <  1:771764/236‑1 (MQ=255)
                                                                                                      gcggcCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCCGCGCAGTACGGCAGGCCATTTCCTGGTTTGGCAACTTTCGGGACCCCCGGGCTA‑CAAAGTGCCCCCTCCCAGGTTTCCCCGCCCGAAATg                                                                                                                                >  2:120505/1‑239 (MQ=255)
                                                                                                       tgttgataagagacagggTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGa                                                                                                                               <  1:553068/223‑1 (MQ=255)
                                                                                                           ccGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATgg                                                                                                                            <  1:250356/238‑1 (MQ=255)
                                                                                                            cGCCCGCATCTGGTTATCGGTCTTGCCTTTGAGCCCTTCTTCTTTGCTGCGCCCGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTg                                                                                                                          <  2:761527/239‑1 (MQ=255)
                                                                                                               caggaggcggttgttcgtccgggcTTTGTGCCCTTCTTTGTTGCGGGGCCCGTCGCTTTCCAGGTTGATGCACTCGGCTGGGTCCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTgcg                                                                                                                        <  2:1099544/215‑1 (MQ=255)
                                                                                                                  cagcagGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCa                                                                                                                    >  1:1293304/1‑239 (MQ=255)
                                                                                                                     cagTTTATCGGTCATGCCTTTGAGCCCTTCTTCTTTGCTGGGCCAGTCGGTTTCCAGGGTGATGCACTCGGCGGTGACCCGCGGTGCGTCGTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGc                                                                                                                 <  2:1123796/239‑1 (MQ=255)
                                                                                                                      agGTTATCGGTCATGCCTTTGAGCCCTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATg                                                                                                                  <  1:907069/237‑1 (MQ=255)
                                                                                                                      agGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCCATCCAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCCTGCt                                                                                                                >  1:173588/1‑239 (MQ=255)
                                                                                                                          ttgttgTCCTGCTTTTTATCATTTCTTTTTTGCCGGCCCAGGCGCTTCCCAGTGTGGTGCACGCGGCGGTGACCAGCGGTGCGTCTTGTGGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAg                                                                                                             <  2:25073/233‑1 (MQ=255)
                                                                                                                          tATCGGTCATGCCTTTGAGCCCTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGt                                                                                                            <  1:913630/239‑1 (MQ=255)
                                                                                                                               ttcttccgttttggcccgttttttttGCCGCCCAAGTCGGTTTCCGGCGGGATGCCCTCGGAGGGGGCCGACGGTGCGGCTTGTTGGGGCATCGGGAATTGCGCCAGCAGGTGTTCGTCTTCCAGCGAAGTACGGCATGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATc                                                                                                       <  2:975821/216‑1 (MQ=255)
                                                                                                                               gttgtgtcTTTGGGCCTTTCTTCTGTGGTGCGGCGGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGGGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACCCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATc                                                                                                       <  2:551511/232‑1 (MQ=255)
                                                                                                                                tcagtgcTTTGAGGCCTTTTTCTTGGCTTGCCCTGTCGGTTGCCGGTGGGATGCCATCGGCGGTTACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATc                                                                                                       <  2:1294702/232‑1 (MQ=255)
                                                                                                                                 tcttccTTTGGCCCCTTTTTCTTGTCTGCGCCCGTCGCTTTCCAGTGTGATGCACTCGGCCGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATcc                                                                                                      <  2:173588/234‑1 (MQ=255)
                                                                                                                                  aTGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTTCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGGGTTCATccc                                                                                                     >  1:511189/1‑237 (MQ=255)
                                                                                                                                  aTGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCa                                                                                                     >  1:112073/1‑238 (MQ=255)
                                                                                                                                  aTGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGGGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACCCAC‑GGCTATCAAGGTGCGCCATCCCAGCTTTTCCCCCCGGAATTGATGGGGCCCCCATGCCGGGtttacccc                                                                                                     >  2:563289/1‑232 (MQ=255)
                                                                                                                                   tGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTTCAGCGAAGTACGGCAGGCCATTGcc                                                                                                                                                                                                    >  2:1191691/1‑143 (MQ=255)
                                                                                                                                   tGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCCTTGCCTGGTTGAGCAACTTGCGGCCCTCAC‑GGCTATCAAGTTGCGCCATCCGGGCTTTCGCCCCCGGCATTGATGGTgcggcactgcttggtttctccag                                                                                                    >  1:636851/1‑218 (MQ=255)
                                                                                                                                   tGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGcc                                                                                                                                                                                                    <  1:1191691/143‑1 (MQ=255)
                                                                                                                                    gCCTTTGACTTCTTCTTCTTTTCTGCGCCGGTCGCTTGCCAGTGTGATGCACTCGGCGGTGACCAGGCGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGcc                                                                                                  <  2:371054/239‑1 (MQ=255)
                                                                                                                                         tGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCCGCAGGGGTTCTACTTCCCGCGAAGTACGGCAGGCCATTGCCCGGTTGAGGCACTTTCGGCCCTCCC‑GGCTATCAAGTTGCGCCATCCCACCTTCCGCCGCCGGAATTGAAGTGGCGCCCAtccctggttccaccaagaccaga                                                                                             >  2:531964/1‑217 (MQ=255)
                                                                                                                                                ttcttcttTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCCGGCCATTGCCTGGTTTAGCAACTTGCGGCCCTCCC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCGGCGTTCATcccaccccaaaccccac                                                                                       >  2:1025262/1‑223 (MQ=255)
                                                                                                                                                       ttgctgggccgttttcTTTCCGGTGGGTTGCCCCGGGCGGTCACCAGGGGGTGGCCTTGTTGGGTACAGCGTAATTGCGCACGCAGGTGTTCTCCTGCCAGCGAATTACGGCAGGCCATTGCCTGGTTGAGAAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACgg                                                                               <  2:595733/224‑1 (MQ=255)
                                                                                                                                                       ttgctcgccccgttgCTTTCCGTTGGTATGCACCTGGCGGTGTCCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTTTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTGCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACgg                                                                               <  2:1098865/225‑1 (MQ=255)
                                                                                                                                                       ttGCTGGTCCAGTCGTTTTCCAGTGGTATGCACTCGGGGGTGACCAGTGGTGCCTCTTGTTGGGTAATGCGGAATTGCGCCAGGAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACgg                                                                               <  1:596542/239‑1 (MQ=255)
                                                                                                                                                       ttGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGGGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCCCCGGAATTTGGTGTGCGGCCATGCTGAGTTCCCCCAGCCCTAACCCCACCCGCAACg                                                                                >  2:745773/1‑238 (MQ=255)
                                                                                                                                                        tgctgcGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGGGCCATCCGAGCTTTCCCCCCCGGAATTGATGGTGCGCCCCTGCCGAGTTCATCCCGCCCCAAGCCCACCAgacaagag                                                                              >  2:1292164/1‑232 (MQ=255)
                                                                                                                                                          ctgcGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACggaa                                                                             >  1:1254847/1‑236 (MQ=255)
                                                                                                                                                          ctgcGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGgca                                                                             >  1:1098865/1‑238 (MQ=255)
                                                                                                                                                               ccAGTCGCTTTCCAGTGGGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACg                                                                        <  1:236244/238‑1 (MQ=255)
                                                                                                                                                                              tgtgATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGGACCTTTCGCTccc                                                         >  1:720695/1‑238 (MQ=255)
                                                                                                                                                                                   tGCACTCTGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGACAGGCCATTGCCTGGTTCAGCAACGTTGGGCCCCCAC‑GGCTATCAAGTTGTGCCATCAGAGCTTTCGCCGCCCGCATTTATAGGCTGCCCAAGCTGGCTTCTTCCAACCCCACACCGCCCAGCCACGGCAGAACAGGACCTTTTGCTCCCaccca                                                    >  2:503736/1‑234 (MQ=255)
                                                                                                                                                                                   tGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCCTTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTTGCCGCCGGCATTGGTGGGTCGCCTATGCTGAGTTTATCCCACCCTAAACCGCCCAGAAACGCCACAACGGAAACCTTCGCCCCCactcc                                                    >  2:281459/1‑234 (MQ=255)
                                                                                                                                                                                   tGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAg                                                                                             <  1:273879/197‑1 (MQ=255)
                                                                                                                                                                                   tGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGGCCAGCAACGGCAGCACGGAACCTTTCGCTCCCACCTc                                                    >  1:1241508/1‑238 (MQ=255)
                                                                                                                                                                                        tcggggtgggccggcgggtggTCTTGTTGGGTCATCGGGAACTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGca                                              <  2:118955/219‑1 (MQ=255)
                                                                                                                                                                                        tCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGca                                              <  1:7271/239‑1 (MQ=255)
                                                                                                                                                                                        gcggcggTGACCAGGGGTGGGTCTTGTTGGGGCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGca                                              <  1:1292164/238‑1 (MQ=255)
                                                                                                                                                                                        cctggggttgcctggggTGCTGCTTGTTGGGTCATGCGGAATGGCGCCAGCAGGTGTTCTACTTCCAGGGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTAGCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGca                                              <  2:1346657/224‑1 (MQ=255)
                                                                                                                                                                                          ggcggTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGGATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGAAACTTGCGGCACTCAC‑GGCTATCAAGGTGCGCCATCCGAGCTTTCCCCGCCGGGATTGATGGGTCGCGCATGCTGAGTTCATCCCGCCCTCAGCCCACCCCACACGGAAGCACGGAACCCTTCcccccccaaccccccccca                                            >  2:887802/1‑226 (MQ=255)
                                                                                                                                                                                          ggcggTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTACCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGGGGGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCCCTCCACCCTCACCGcaca                                            >  1:1351257/1‑239 (MQ=255)
                                                                                                                                                                                                 ctcggCGGGGCGTCTTGTTGGTTCTGGCGGAATTGCGCAAGCAGGTGTTCTACTACCAGGGAAGTACGGCAGGCCATTGCCTGGTTGGGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAAt                                     <  2:981765/235‑1 (MQ=255)
                                                                                                                                                                                                     gCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGGGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCACTCCCAACTAACCGAACAGACCAAACCaa                                 >  2:664356/1‑238 (MQ=255)
                                                                                                                                                                                                       ggTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCAtt                                <  1:1169737/238‑1 (MQ=255)
                                                                                                                                                                                                         tGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTACGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCACCTCACCGCCCAGACCAACCCATTTg                              >  2:373575/1‑238 (MQ=255)
                                                                                                                                                                                                           cGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCCCTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCCCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGCCCAGCAACAGCAGCACGGAACCTTTCGCTCCCACCGCCCCCCACCaaccaaaccactccccc                           >  2:876233/1‑232 (MQ=255)
                                                                                                                                                                                                           cGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGTCGCCGGAATTGATGGTGCGCTCATGCTCAGTTCATCCAGCCCTAAGCCGACAAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCAtttcc                             >  1:82962/1‑235 (MQ=255)
                                                                                                                                                                                                            gTCTTGTTGGGTCATGCGGAAGTGCGCCAGGAGGTGTTCGACTTCCATGGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCGC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGccc                           <  1:1359030/238‑1 (MQ=255)
                                                                                                                                                                                                              tttgttgGGTCATGCGGAATTGCGCCAGCAGGGTTTCTACTTCCAGGGAAGTTCGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTgg                        <  1:120505/238‑1 (MQ=255)
                                                                                                                                                                                                                    gggtgagggggAATTTCGCCTGCAGGTGTTCTACTTCCAGGGAAGTACGGCAGGCCATTGCCGGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGc                  <  2:720695/230‑1 (MQ=255)
                                                                                                                                                                                                                       tCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCCCGGAAACTTTCGTTCCCTGCTCACCGCCCAGACCAATCCATTGACCCCGGCgcggacac               >  2:100005/1‑232 (MQ=255)
                                                                                                                                                                                                                          tGCGGAATTGCGTCAGCAGGTTGTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGc             <  1:535080/238‑1 (MQ=255)
                                                                                                                                                                                                                          tGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCACGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGAAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGACCTGGCGATGCACCGCt            >  1:407620/1‑239 (MQ=255)
                                                                                                                                                                                                                          tGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTg                                                                                                                          >  1:1279300/1‑129 (MQ=255)
                                                                                                                                                                                                                             ggAATTGCGGCAGCAGGTGTTCTACTTCCAGGGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTg          <  1:1207751/238‑1 (MQ=255)
                                                                                                                                                                                                                              gAATTGCGCCCGCAGTGTTTCTTGTTCCCGCGAAGTCCGGCCGGGCTTTGCCTGGTTGAGCACCTTGCGGCAATCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCa        <  2:478603/239‑1 (MQ=255)
                                                                                                                                                                                                                               aaTTGCTCCAGCAGGTGTTCGACTTCCAGCTAAGTACGGCAGGCCATTGCCTGGTTGGGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCa        <  1:100005/238‑1 (MQ=255)
                                                                                                                                                                                                                               aaTTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCa        <  1:833111/238‑1 (MQ=255)
                                                                                                                                                                                                                               aaTTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCa        <  1:745773/238‑1 (MQ=255)
                                                                                                                                                                                                                                 ttGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCa                                                        >  2:326293/1‑188 (MQ=255)
                                                                                                                                                                                                                                 ttGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCa                                                        <  1:326293/188‑1 (MQ=255)
                                                                                                                                                                                                                                    cgcCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCCCGGCGt  >  1:207509/1‑239 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                     
TCTGATTTGCTGTGTGGAATGGCAAAACTAAAGCCCAGACCGGTAGAGAAAA‑CGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAC‑GGCTATCAAGTTGCGCCATCCGAGCTTTCGCCGCCGGAATTGATGGTGCGCTCATGCTGAGTTCATCCAGCCCTAAGCCGACCAGCAACGGCAGCACGGAACCTTTCGCTCCCAGCTCACCGCACAGACCAATCCATTTGCCCTGGCGATGCACCGCTTGCACGGCGT  >  NC_000913/4139960‑4140425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A