Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,617,437 T→C 14.0% T38T (ACT→ACC)  deoC → 2‑deoxyribose‑5‑phosphate aldolase, NAD(P)‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,617,4370TC14.0% 297.7 / 4.0 122T38T (ACT→ACC) deoC2‑deoxyribose‑5‑phosphate aldolase, NAD(P)‑linked
Reads supporting (aligned to +/- strand):  ref base T (85/15);  new base C (7/15);  total (92/30)
Fisher's exact test for biased strand distribution p-value = 1.56e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

TGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCA  >  NC_000913/4617200‑4617670
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          
tGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGC‑CGAGAAAGGGATAGCCCTG‑TGTCATCAGGCCAAAACTc                                                                                                                                                                                                                                           >  1:48334/1‑239 (MQ=255)
  ttGCAAACTTGTAAGTGGTTTTCCTTAATTGTGAGTTGTATCGAAGTGTTT‑TTCGGAGTAGAGGTTAGAATACTACAAAACTCGCAAGGTGAATTTTATGGGCG‑ACAAGCCAGGAGAATGAAATTACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑g                                                                                                                                                                                                                                        <  2:1248325/238‑1 (MQ=255)
  aTGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGCGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACCCC‑g                                                                                                                                                                                                                                        >  1:606922/1‑239 (MQ=255)
  aTGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGGGAATTTTATTGGCG‑ACAAGCCAGGCGAATGAAATGACTGATCTGAAAG‑CAAGCCGCCTGCGT‑GCC‑CTGAAATTGATGGACCTGACCACCCTG‑ACTGACGACGACACCGA‑CGAGAAAGTGAGCGCCCTG‑TGTCATCAGGCCAAAAATCC‑g                                                                                                                                                                                                                                        >  1:698691/1‑239 (MQ=255)
   tGCAAACTTGTAAGTAGATTTCCTCAATTGTGATGTGTATCGCAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGCGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGCCCTAACCACCCTG‑AATGACGCCGACACCGA‑CGAAAAAGTGAGCGCCCTG‑TGCCATCAGGCCAAAACCCC‑ga                                                                                                                                                                                                                                       >  2:1363972/1‑238 (MQ=255)
     ggatgttttaaaggtgaagccTTAATTGGGATGTGTATGGAAG‑GTGTGTGGGGAGGAGAAGTTAGAATTCTAACAAACGCGCAAGGGAAATTTTAGTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCGGAAAG‑CAAGCAGCCTGCGT‑GCA‑TTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTc                                                                                                                                                                                                                                     <  2:1077638/220‑1 (MQ=255)
        gtttGGAAGTATTTTTCTTTAATTGGGTTGTGTATCAGAGTGTGT‑TGGGGAGTAGATGTTAAAATCCTAAAAAATGCGAAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAAGGAAATGACTTATCTGAAAG‑CAAGCAGCCTGCGG‑GCA‑CTGAAATTGATGGACCTGCCCCCCCTG‑AATGACGACGACCCCGA‑CGAGAAAGTGATCGCCCTG‑GGTCATCAGGCCAAAACTCC‑GGTCgg                                                                                                                                                                                                                                   <  2:42388/236‑1 (MQ=255)
            gTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAAt                                                                                                                                                                                                                               >  1:953853/1‑238 (MQ=255)
                    aTTTCCTTAATTGTGATGTGTATCGAAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGCACTGACCACCCTG‑AATGACGACGACACCGC‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCt                                                                                                                                                                                                                      >  1:42388/1‑239 (MQ=255)
                                 tgATGTGTATCGAAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGGGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGCCACCGA‑CGAGAAAATGATCACCCCG‑TGCCTTCAGGCCAAAAACCC‑GGCCGGCAAAAcccccccactccgtttccaa                                                                                                                                                                                                          >  2:444083/1‑223 (MQ=255)
                                   aTGTGTATGGAAGTGTTT‑TGGGGAGTAGATGTTAGAATACTAAAAAACTCGAAAGGTAAATTTTATTGGCA‑ACAAGCCGGGAAAATGAAATGACTGATCTAAAAG‑CAAGCAGCCTGGGT‑GCA‑CTGAAATTGATGGACCGGCCCCCCCTG‑AAGGACGACGACCCCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATcc                                                                                                                                                                                                        <  1:398017/238‑1 (MQ=255)
                                   aTGTGTATCGAAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAGATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATcc                                                                                                                                                                                                        >  1:1074059/1‑238 (MQ=255)
                                   aTGTGTATCGAAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGCGAAAGTGATCGCCCTG‑TGTCATCAGGCCCAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATcc                                                                                                                                                                                                        >  1:123655/1‑238 (MQ=255)
                                   aTGTGTATCGAAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATAccg                                                                                                                                                                                                                           >  1:1077638/1‑219 (MQ=255)
                                        tATCGAAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GGA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CAAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAACACCC‑GGTCGGCAATACCGCCGCTTTCTAGATCTATCCTCGCtt                                                                                                                                                                                                  >  1:9860/1‑239 (MQ=255)
                                        tATCGAAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCtt                                                                                                                                                                                                  >  1:1013268/1‑239 (MQ=255)
                                        tATCGAAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGCATCTATCCTCGCtt                                                                                                                                                                                                  >  1:477321/1‑239 (MQ=255)
                                        tATCGAAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAAAACCGCCGCTATCTGaat                                                                                                                                                                                                              >  1:612903/1‑227 (MQ=255)
                                                tgtgt‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACCCCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGCCGGCAAAACCGCCGCCATCTGTATCTATCCCCGCTTTATCCCGa                                                                                                                                                                                          >  2:866197/1‑239 (MQ=255)
                                                tgtgt‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGGGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGa                                                                                                                                                                                          >  1:1349103/1‑239 (MQ=255)
                                                tgtgt‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGGGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTACCTATACTCGCTTTATCCCgt                                                                                                                                                                                          >  1:149895/1‑238 (MQ=255)
                                                  tgt‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGGTCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGAAGACG‑CACCCACCGAGAAAGTGAGCACCCTG‑AGACACCCGGCCAAAACTCC‑GAGCGGGCAAACAGCCCCCATCTGTATCTATCCTCGCTTTATACCgctg                                                                                                                                                                                        >  2:689100/1‑236 (MQ=255)
                                                  tgt‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGtatct                                                                                                                                                                                                            >  1:23516/1‑219 (MQ=255)
                                                  tgt‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGAtt                                                                                                                                                                                        >  1:1090151/1‑239 (MQ=255)
                                                  tgt‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGAtt                                                                                                                                                                                        >  1:877552/1‑239 (MQ=255)
                                                  tgt‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGGGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGCCGGCAAAACCACCGCTAACTGTATCCATCCCCCCTTTACCCCCAtt                                                                                                                                                                                        >  2:611433/1‑239 (MQ=255)
                                                  tgt‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGGGATCGCCCTG‑CGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATTTGAATCTATCCTCGCttttaccctggc                                                                                                                                                                                        >  1:391787/1‑230 (MQ=255)
                                                    t‑tGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGCCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTg                                                                                                                                                                                       >  1:205443/1‑238 (MQ=255)
                                                        cGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAT‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGCGAAAGTGATCGCCCTG‑TGTCATCGGCCCAAACCCCC‑GGCCGGAAACACCCCCGCTTTCTGTACCTAACCCCCCTTTCTCCCgcattatcc                                                                                                                                                                                   >  2:14558/1‑231 (MQ=255)
                                                         ggagaggATGTAAGAATACTAACAAACTGGAAAGGTGATTTTTATGGGCG‑AAAAGCAAGGAGAATGAAATGACTAAACGAAAAG‑AAAGCAGCTGGGGT‑GCA‑CTAAAATGGAGGGCCCTGCCCCCCCGA‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCAAAACCTCC‑GGCCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCg                                                                                                                                                                                   <  1:1337471/232‑1 (MQ=255)
                                                           aGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCCCaa                                                                                                                                                                                >  1:620703/1‑239 (MQ=255)
                                                            gTAGTTGTTAAAATACAAAAAAACCCGAAGGGTAAATTTTATTGGCG‑AAAAGCCAGGAAAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCGGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCaa                                                                                                                                                                                <  1:140014/238‑1 (MQ=255)
                                                                  gTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTTAATCTATCCTCGCTTTATCCCGATTGCTCCCAAAACCCTg                                                                                                                                                                         >  2:140014/1‑239 (MQ=255)
                                                                  gTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGCATCTATCCTCGCTTTATCCCGATTGCTCGCCAAACTCTg                                                                                                                                                                         >  1:346703/1‑239 (MQ=255)
                                                                     aGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGCCGGCAATACCGCCCCAATCTGTATCTATCCTCCCTTTATCCCCATTCCCCCCAAAAACCTGaaa                                                                                                                                                                      >  2:228200/1‑239 (MQ=255)
                                                                       aaTACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAaga                                                                                                                                                                    >  1:1191400/1‑239 (MQ=255)
                                                                       aaTACTAACAAACTCGCAAGGAGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCGGCTATCGGTATTTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAaga                                                                                                                                                                    >  1:27507/1‑239 (MQ=255)
                                                                         tACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGaa                                                                                                                                                                       >  1:317160/1‑234 (MQ=255)
                                                                            tAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAg                                                                                                                                                                >  1:502650/1‑238 (MQ=255)
                                                                             aatatacccgaagtgtgtATTTTTTGGGGA‑ACAAGCCAGGAGAATTAAATGACTGATCGAAAAG‑AAAGCAGCCCGGTG‑GCA‑CTAAATTGGAGGGCCCTCACCCCCCGG‑AATGACGACGACACCGA‑AGAGAAAGTACCCGCCGTG‑TGTAACCCGGACAAAGCTCC‑GGTCGGAAATGCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAggg                                                                                                                                                              <  2:584588/225‑1 (MQ=255)
                                                                             aaaaaaCTCG‑AAAGTGTTTTTTTTTGGGGAACAAGCCAGGAAATAGAAATGACTGATC‑GAAAGAAAAGCCGCC‑GTGTGGAA‑CTAAAATGGA‑GGACCCGACCCCCCCGAAATGACGCCGACCCCGA‑GGAAAAAGTGATCGCCCTG‑TGTCACCAGCCAAAACCCCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAggg                                                                                                                                                              <  2:1130813/236‑1 (MQ=255)
                                                                              aCAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGCGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAACAggg                                                                                                                                                              >  1:1097693/1‑238 (MQ=255)
                                                                              aCAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTGGCAAAACTCTgcacgacaaggg                                                                                                                                                              >  1:942396/1‑227 (MQ=255)
                                                                                              tcaagtttgttaaaaaggaatgggAAAAAGAAATGACTGGTCTAAAAG‑AACCCGGCCTGGGT‑GCA‑CTAAAATTGAGGGCCCTGCCCCCCCAG‑AATAGCGACGCCCCCGA‑CGAAAAAGTCCCCCCCTGG‑TTTCACCAGCCCAAACCCCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCg                                                                                                                                               <  2:963764/216‑1 (MQ=255)
                                                                                              aaTTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAAACCAGGGCACCCCGGAAATCCgaa                                                                                                                                             >  2:278985/1‑237 (MQ=255)
                                                                                                     tggggccaacaacgagaaaaatgaaatgaATGGTCAAAAAG‑ACAGCCGCGTGCG‑‑GCACCAGAAATTTAGGGCCCTGCCCCCCCTG‑AAGGACGACGTCCCAGA‑CGAAAAAGGCCCGGCCCTG‑TGTCAGCAGGCCAAAACTCC‑GGCCGGCAATTCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTa                                                                                                                                       <  2:1013268/220‑1 (MQ=255)
                                                                                                                          tgaaatgaCTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACGGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGAACCCCGAAAATCCGTATCGCTACGGTAACCAACTTCCCACACg                                                                                                                  >  1:682610/1‑239 (MQ=255)
                                                                                                                          tgaaatgaCTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTTTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCCCCCCGGAAATCCGTATCCCTACGGAAACCAACTTCCCacacc                                                                                                                  >  2:258089/1‑238 (MQ=255)
                                                                                                                          tgaaatgaCTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACg                                                                                                                  >  1:101218/1‑239 (MQ=255)
                                                                                                                          tgaaatgaCTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACg                                                                                                                  >  1:221292/1‑239 (MQ=255)
                                                                                                                          tgaaatgaCTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCCCTACGGTAACCAACTTCCCCCACg                                                                                                                  >  1:1187855/1‑239 (MQ=255)
                                                                                                                          tgaaatgaCTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCCCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCCAAACTCTGAAAGAGCCGGGCCCCCCGGAAATCCGTAACGCTAAGGAAACCAACACCCCAAACg                                                                                                                  >  2:714787/1‑239 (MQ=255)
                                                                                                                           gaaatgaCTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGAGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCCCTACGGTAACCAACTTCCCAAACgg                                                                                                                 >  1:813250/1‑239 (MQ=255)
                                                                                                                               tgaCTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACg                                                                                                                                     >  1:963764/1‑215 (MQ=255)
                                                                                                                                  cTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAacgac                                                                                                          >  1:761284/1‑239 (MQ=255)
                                                                                                                                   tGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGAtt                                                                                                                                                                                        >  1:614828/1‑160 (MQ=255)
                                                                                                                                      tCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGTGAACCCCGGAAATCCGAATCGCTACGTTAACCAACTTCCCACACGGTAACGacaaca                                                                                                      >  1:833974/1‑239 (MQ=255)
                                                                                                                                          aaaG‑CAAGCAGCCAGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACCCC‑GGTCGGCAAAACCGCCGCTATCTGTATCTATCCTCGCCTTATCCCCATTGCTAGCAAAACTCTGAAAGACCAGGGCCCCCCGGAAATCCGGATCCCTGCGGTAACCAACTTCCCCCAAGAGACCgacgacccaa                                                                                                   >  2:744111/1‑234 (MQ=255)
                                                                                                                                                aagcaGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGGAACGACgaaatcgacatc                                                                                              >  1:1037452/1‑238 (MQ=255)
                                                                                                                                                 agcagcCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACCCGGTAACGACGccatcgacaccg                                                                                             >  1:1044546/1‑238 (MQ=255)
                                                                                                                                                  gcagcCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTACCCAACTTCCCACACGGTAACGACGAcatcgccatcgc                                                                                            >  1:1095507/1‑238 (MQ=255)
                                                                                                                                                  gcagcCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCaa                                                                                                                             >  1:844192/1‑205 (MQ=255)
                                                                                                                                                   gagcCCGCGT‑CCA‑CTAAAATTGATGGCCCTGCCCACCCTG‑AATGACGACGACCCCGA‑CGAAAAAGTGACCGCCCTG‑TCTCATCAGCCCAAACCTCC‑GGCCGGCAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCgcgc                                                                                          <  1:866197/238‑1 (MQ=255)
                                                                                                                                                      gcctggggcgcacgaaaaTGGGTGGACCCCCCCACCCAG‑AACGACACCGCCACCGA‑CGAAAAAGTGACCGCGCGG‑TTTCACCAGCCAAAAACCCC‑GGCCGGCAATACCGCCGCTATCTGTATCTAGCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCgcgc                                                                                          <  2:984837/223‑1 (MQ=255)
                                                                                                                                                         gCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGCTAACGACGACATCGCCATCCCGCTGCCAg                                                                                    >  1:511711/1‑239 (MQ=255)
                                                                                                                                                            t‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCCGaa                                                                                  >  1:130058/1‑238 (MQ=255)
                                                                                                                                                                     aaaTTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAACTCCGTATCGCTACGGTAACCAACTTCCCACCCGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGtcc                                                                          >  1:656946/1‑237 (MQ=255)
                                                                                                                                                                         agtgttttataagaaccagg‑AATGCCGCCGACCCCGG‑GAAAAATGTGACCCCCCTG‑TCTCAAAAGGACAAAACCCG‑GGCCAGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTGCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCa                                                                      <  2:1055315/220‑1 (MQ=255)
                                                                                                                                                                           aTGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATTTATCGTCGCTTTATCCCGATTGCTCTCAAAACTCTAAAAGAGCAGGGCACCCCGGAAAGCCATATCGCTACGGTAACCAACTTCCCACACGGTAACGCCGCCAGTGACAATGCGCAGGCAGAAAcccacgggacaaa                                                                    >  2:820051/1‑229 (MQ=255)
                                                                                                                                                                               aCCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACAGTAACGCGGCCATCTACACCGCGCGTGCAAAACCCCAGGCGGaaaaccc                                                                 >  2:58572/1‑234 (MQ=255)
                                                                                                                                                                               aCCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGAGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGCCAACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAAACGc                                                                 >  1:269092/1‑238 (MQ=255)
                                                                                                                                                                                    accaccCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGTCACCCCGGAAATCCGTATCGCTCCGGTAACCAACTTCCCACACGGTAACGACGCCATCGACAACCCGCGGCCAGAAACCCGTGCGGCAATCGCCAACgg                                                           >  2:37481/1‑239 (MQ=255)
                                                                                                                                                                                         ccTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCCCACGGTAACGACGCCCTCGACATCGCGCTGGCACAAACCCGTGCGGCAAACGACAACGgtggtg                                                      >  2:37302/1‑239 (MQ=255)
                                                                                                                                                                                         ccTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTG‑‑‑‑TATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGccctgtcccttataca                                                  >  1:279111/1‑225 (MQ=255)
                                                                                                                                                                                           tG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACCACGACCTCGACACCGCGCTGGCACAAACCCCGGCGGCAATCGCCAACggggggagg                                                    >  2:754822/1‑232 (MQ=255)
                                                                                                                                                                                            caattcgccgcgacCCCGT‑CGAGAAATTCGTCCCCCTG‑GTTCATCTGCCAAAACCTCC‑GGTCGACATGACCGCCGCTATCTGTATCTTACCCCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCtgat                                                    <  2:306948/229‑1 (MQ=255)
                                                                                                                                                                                                   cgacgacACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGtt                                              >  1:747112/1‑238 (MQ=255)
                                                                                                                                                                                                      cgccacCGG‑CTAGAAGGTGCCCCCCTTT‑TGTCACAACGCAAAACCCCC‑GGTCGGAAATCCCGCCGCTATCTGTATCTATCCCCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTCCCCACACGGTAACGACGACAGCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACg                                          <  2:88851/236‑1 (MQ=255)
                                                                                                                                                                                                        acacCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACCCGGTAACGCCGCCATCGACATCCCGCTGGCAGAAACCCGTGCGGCAACCGCCTACGGGGCTGATGAAGTTGACGtt                                        >  1:680061/1‑239 (MQ=255)
                                                                                                                                                                                                        acacCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACggctgtcccttatacacatc                                         >  1:1055315/1‑220 (MQ=255)
                                                                                                                                                                                                         ccccGA‑AGAAAAGGTGACCCCCCTG‑TGTCATCAGGCCAAACCCCC‑GGTCGGCAATTCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTtg                                       <  1:228920/237‑1 (MQ=255)
                                                                                                                                                                                                         acccgc‑cgAGAAAGTTCTCCCCTTG‑GTTCCACAGGCAAACACCCC‑GGGCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTtg                                       <  1:346982/237‑1 (MQ=255)
                                                                                                                                                                                                           ccgggagagaAAGGTATCGTCGTG‑GTTCATCAGCCCAAACCTCG‑GGTGGGCAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTtg                                       <  1:37302/232‑1 (MQ=255)
                                                                                                                                                                                                           cccgcacgaaaaGTGCTCCCCCTGTTTTCATCAGCCCAAACCCCG‑GGTCGCAAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTtg                                       <  1:857602/230‑1 (MQ=255)
                                                                                                                                                                                                            ccgaaggaaaaggtctccccgtg‑tgTCATACGCCCAAACCCCA‑GCTCGAAAACACCGCCGCTTTCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTtgtg                                     <  2:1044546/218‑1 (MQ=255)
                                                                                                                                                                                                                          aTCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGAAATCGCCAACGGTGGTGAGAAAGGTGAGGtttgtttcacgaaacccgc                       >  2:346750/1‑222 (MQ=255)
                                                                                                                                                                                                                              ccttg‑tgCCACCAGCCCAAATCCTC‑GGCCGGAAACACCGCCCTTATCTGTAACTATCCCCGCCTTAACCCGATTGCTCGCAAAACTCCGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTg                   <  2:351075/236‑1 (MQ=255)
                                                                                                                                                                                                                              cccTG‑TTTCAGCCGCCAAAAACTCC‑GGTCGACAATGCCGCCGCTATCTGTATCTAGCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTg                   <  1:278985/239‑1 (MQ=255)
                                                                                                                                                                                                                                  g‑tgtCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGtat                                                                                                                                                                                                              >  2:157246/1‑48 (MQ=255)
                                                                                                                                                                                                                                  g‑tgtCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTCCGTGGCTGATGAAGTTGCCGTTGGGTTCCCGTACCGCGAGCGGATgg               >  2:1066002/1‑239 (MQ=255)
                                                                                                                                                                                                                                  g‑tgtCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATgg               >  1:340420/1‑239 (MQ=255)
                                                                                                                                                                                                                                  g‑tgtCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCCCGCGCTGATgg               >  1:767123/1‑239 (MQ=255)
                                                                                                                                                                                                                                  g‑tgtCATCAGGCAAAAACCCC‑GGTCGGCAATACCGCCGCTATCTGtat                                                                                                                                                                                                              <  1:157246/48‑1 (MQ=255)
                                                                                                                                                                                                                                     ctcaccaGGCAAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGc              <  1:444083/237‑1 (MQ=255)
                                                                                                                                                                                                                                             gCCAAAACTCC‑GGTCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAACAGCAGGGCACCCCGGACATCCGTATCGCTACGGTAACCAACTTCACACACGGTAACGCCGCCATCCACACCGCGCTGGCAGACACCCGTTCGGCAATCCCCTACGGGCCTGGTGAAGTTGAAGGTGGTTTCCCGAACCGCGCTCTggctgcggggaaggg     >  2:1178903/1‑225 (MQ=255)
                                                                                                                                                                                                                                              ccAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAACGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCCCCTACGCGGCTGTTGAGGTTGACGTTGTGTTCCCGTACCGCCCGCTgtagggggggtacgcg    >  2:1173561/1‑224 (MQ=255)
                                                                                                                                                                                                                                              ccAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACg                                                            >  1:626271/1‑183 (MQ=255)
                                                                                                                                                                                                                                              caaaacCTCC‑GGCCGCAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAg    <  1:754822/237‑1 (MQ=255)
                                                                                                                                                                                                                                               cAAAACCCCGGGGCGGAAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAg    <  1:258089/239‑1 (MQ=255)
                                                                                                                                                                                                                                                aaaaCTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGCCATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGGGCTGATGAAGTTGCCGTTGTGTTCCCGAACCCCGCCCTGcgggcgggggacaggca  >  2:495983/1‑230 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          
TGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGT‑TGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCG‑ACAAGCCAGGAGAATGAAATGACTGATCTGAAAG‑CAAGCAGCCTGCGT‑GCA‑CTGAAATTGATGGACCTGACCACCCTG‑AATGACGACGACACCGA‑CGAGAAAGTGATCGCCCTG‑TGTCATCAGGCCAAAACTCC‑GGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCA  >  NC_000913/4617200‑4617670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A