Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,633,068 T→G 11.1% V103G (GTG→GGG)  trpR → transcriptional repressor, tryptophan‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,633,0680TG11.1% 311.5 / 2.4 124V103G (GTG→GGG) trpRtranscriptional repressor, tryptophan‑binding
Reads supporting (aligned to +/- strand):  ref base T (30/71);  new base G (19/2);  total (51/73)
Fisher's exact test for biased strand distribution p-value = 2.52e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

ACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGC  >  NC_000913/4632831‑4633292
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                 
acaggcctAAGGATGCCTCCCAAAACGGACCCCATTTACCGTTGGTAAACCTGGTGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGGGCGTATTGTCGAAGAGCTGTTGCGCGGGGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGgtg                                                                                                                                                                                                                                  <  1:1277758/232‑1 (MQ=255)
 ccTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTGGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGgtg                                                                                                                                                                                                                                  <  1:1279871/238‑1 (MQ=255)
     cTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAATCCGCGCCCGTCGAG‑CTGCGCCAGGGGCGGGAAGGGGGGtt                                                                                                                                                                                                                                >  1:1352954/1‑236 (MQ=255)
               ccTTCCAAAACGATCTCCATTTACCGTTGTCAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCgat                                                                                                                                                                                                                   <  1:910789/239‑1 (MQ=255)
               ccTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGGGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGGGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGGGGCTGGAAGGGGGGTTGTGGGAAAGGgat                                                                                                                                                                                                                   >  2:1100523/1‑239 (MQ=255)
               ccTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAATATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCgat                                                                                                                                                                                                                   >  1:874952/1‑239 (MQ=255)
               ccTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGCTTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGGCCCAGGGGCTGGAAGAGGGGTTGCTGAAAAggggg                                                                                                                                                                                                                   >  2:1346255/1‑235 (MQ=255)
               ccTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCGGGGGCGGGAAGAGGGGGGGCGGAAAAgggtt                                                                                                                                                                                                                   >  2:722556/1‑235 (MQ=255)
               ccTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGGGGTGTTGCTGAAAAGCgat                                                                                                                                                                                                                   >  1:1364668/1‑239 (MQ=255)
               ccTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGGGGCTGGAAGAGGGGTTGCTGacaacggat                                                                                                                                                                                                                   >  1:1049641/1‑234 (MQ=255)
               ccTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCG‑CCGTCCAGCCTGCGCCAGGGGTGGGAGGGGGGGTGGCGGAAAAgatg                                                                                                                                                                                                                    >  2:1277758/1‑235 (MQ=255)
               ccTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAACCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGGAAAACgat                                                                                                                                                                                                                   >  1:1318623/1‑239 (MQ=255)
                  aCCAAAACGATCGCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCgattga                                                                                                                                                                                                                <  1:468885/239‑1 (MQ=255)
                       aaCGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTg                                                                                                                                                                                                           <  1:1100523/239‑1 (MQ=255)
                        aCGATCCCCATTTACCGTTGTTAAACCTGGAGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGt                                                                                                                                                                                                          <  1:167579/239‑1 (MQ=255)
                         ccctctcCATTTACCGTTTTTAAACCTGATGCTGACGCCAGATGAGCG‑CGGAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGGTGTTGCGCGGCGAAATGGGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTa                                                                                                                                                                                                         <  2:1173357/236‑1 (MQ=255)
                         cGATCTCCATTTACCGTTGTTAAACCTGATGCTGCAGCCAGATGAGCGCCGAGGCG‑TGGGGACTCGCTTGCGTATTGTCGAAGAGTTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTa                                                                                                                                                                                                         <  1:937913/239‑1 (MQ=255)
                          gATCCCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGGGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAg                                                                                                                                                                                                        <  1:1366094/239‑1 (MQ=255)
                            ttcccATTTGCCTGTGTTTAACCTGTTGCTGGCCGCAGTTTGTCG‑CGAGTCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGc                                                                                                                                                                                                      <  2:1197000/236‑1 (MQ=255)
                            tctcatttttCCGTTTTGAAACCTGAGGCTGGCGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGc                                                                                                                                                                                                      <  2:182908/233‑1 (MQ=255)
                            tctcCATTTGCCGTTGGTAAACCTTGTGCTGGCGCCTGATGAGCG‑CGGAGCGGTGGGGACTCGCGTGCGTATGTTCGAAGAGCTGTGGCGCGGCGAAATGAGCCAGCGTGAGGTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAgg                                                                                                                                                                                                       <  2:1025299/238‑1 (MQ=255)
                            tctcCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGCCGAG‑CTGCGCCATGGGCTGGAAGAGGGGTTGCTGGAAAGCGATTGATTTTGTAgg                                                                                                                                                                                                       >  2:468885/1‑238 (MQ=255)
                            tccccATTTCCCGTTGTTAAACCTGATGCTGCAGCCGGATGGGCG‑CGAAGCGTTGGGGACCCGCGTGCGTATTGGCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGc                                                                                                                                                                                                      <  2:1358888/236‑1 (MQ=255)
                            tccccATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGc                                                                                                                                                                                                      <  1:1164629/236‑1 (MQ=255)
                              tcCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGccg                                                                                                                                                                                                    >  1:430079/1‑238 (MQ=255)
                                 aTTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGAt                                                                                                                                                                                                 <  1:291941/239‑1 (MQ=255)
                                          tgttAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTg                                                                                                                                                                                         >  1:859367/1‑238 (MQ=255)
                                          tgttAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAACCGTgg                                                                                                                                                                                        >  1:372238/1‑239 (MQ=255)
                                          tgttAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAAAGGGGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGACAAAACgggg                                                                                                                                                                                        >  1:561980/1‑239 (MQ=255)
                                          tgttAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGAGGCTGGAAGAGGTGTTCCTGAAAAGCGATTGATTTTTTAGGCCTGGTAAGACGTg                                                                                                                                                                                         >  1:84681/1‑238 (MQ=255)
                                          tgttAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAACCGCGCCCGTCGAG‑CTGCGCCAGGGGCTGGAAGAGGGGTTGTGGAAAACGGATTGTtttttagggcgggaaaagaagggg                                                                                                                                                                                        >  2:712349/1‑219 (MQ=255)
                                          tgttAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGGGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGGGTTGCGGAAAAGCGATTGATTTTTTGGGCCTGTTAAGACGTgg                                                                                                                                                                                        >  1:425753/1‑239 (MQ=255)
                                           tttAACCCTGTTGCTGACGCCAGATGGGGG‑CGAAGGGTTGGGGACTCGCGTGCGTATTGTAGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGgc                                                                                                                                                                                       <  2:1212467/238‑1 (MQ=255)
                                           gttAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGgc                                                                                                                                                                                       <  1:712349/239‑1 (MQ=255)
                                           gttAAACCGGTTGGTGAGGCCAGAGGGGCG‑GGAAGCGTGGGGGACCGGCGTGCGTATTGTCGAAGAGCGGTGGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACGCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGgc                                                                                                                                                                                       <  2:744970/239‑1 (MQ=255)
                                               aaCCTTATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCAt                                                                                                                                                                                   <  1:1141844/239‑1 (MQ=255)
                                                    gATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CGGCGCCAGTGGCTGGAACAGGTGTTGCTGAAAAGCGCTTGATTTTTTAGGCCTGGAAAGACGGGgggcctggggg                                                                                                                                                                              >  2:1361983/1‑232 (MQ=255)
                                                    gATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGAGCCCGTCGAG‑CTGAGCCAGTGGCTGGGAGGGGGGTTGCGTAAAAGCGGTTTTTTTTTTAAGCCCGGAAAGAAGTgggcgcaccggg                                                                                                                                                                              >  2:41199/1‑229 (MQ=255)
                                                     aTGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGGAGGGGTGTTGGCGAAAAACGCGTGATTTTGTAGGCCGGCAAAGAAGGAGCGCAACAGACa                                                                                                                                                                             >  2:137214/1‑239 (MQ=255)
                                                     aTGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCa                                                                                                                                                                             >  1:959636/1‑239 (MQ=255)
                                                     aTGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGCCGAG‑CTGCGCCAGGGGCTGGCAGGGGGGTTGCTGGAAAGCGCTTGCTTTTTGAGGCCGGAAAAGGCGTGGGGCAtagggga                                                                                                                                                                             >  2:252742/1‑233 (MQ=255)
                                                           aCGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGTGGAGTTGCTGAAAAGCGATTGATTTTGTa                                                                                                                                                                                                         >  1:640150/1‑205 (MQ=255)
                                                           aCGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGGGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGc                                                                                                                                                                       >  1:854655/1‑239 (MQ=255)
                                                            cGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGGTGCTGACAAGCGATTGATTTTTTTGGCCTGGTAAACAGGGGCCCATCAgggcacgggg                                                                                                                                                                       >  2:323686/1‑230 (MQ=255)
                                                                                  tGGGGTCTCGGGGGCGTTTTGTCGAAGGGCTGTTGCGCGGGGAAAGGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTg                                                                                                                                                <  2:372238/239‑1 (MQ=255)
                                                                                  tGGGGGCTCGGGTGCGTATTGTAGAAACGCTTTTGGGCGGCGAAATGAGCCAGCGTGAGTTAAAAAAGGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTg                                                                                                                                                <  2:1352954/239‑1 (MQ=255)
                                                                                  tGGGGACTCGGTTGCGTTTTGTCGAAGAGCTGGTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTg                                                                                                                                                <  2:301328/239‑1 (MQ=255)
                                                                                  tGGGGACTCGCGTGGGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACCCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTg                                                                                                                                                <  1:723100/239‑1 (MQ=255)
                                                                                  tGGGGACGCGCATGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTg                                                                                                                                                <  1:534107/239‑1 (MQ=255)
                                                                                   tgggcctcggttgcgtttttgggAAGAGCTGTTGGGCGGCGAAATGGGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGa                                                                                                                                               <  2:580831/217‑1 (MQ=255)
                                                                                   ggggACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGGGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTg                                                                                                                                                <  1:1298419/238‑1 (MQ=255)
                                                                                        cTCGCGTGCGTATTGTCGAAGAGCTGGTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGc                                                                                                                                           <  1:722556/238‑1 (MQ=255)
                                                                                         tCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGCTGCGGCGTGAACGCCt                                                                                                                                         >  1:745356/1‑239 (MQ=255)
                                                                                         tCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGGGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAAACGGGGGGCATCAGGCATCGTGCACC‑GAAAGCCGGATGGGGCGGGAACGCCt                                                                                                                                         >  2:633877/1‑239 (MQ=255)
                                                                                           gcgtgcgtATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                       >  1:161896/1‑239 (MQ=255)
                                                                                            ggtgcgtATTGGCGAAGCGCTGTTGCGCGGGGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                      <  1:1346255/238‑1 (MQ=255)
                                                                                            cgttggttttttcaaaggggttttGCGCGGCGAAATGAGCCAGGGGGATTTAAAATTAGAACGCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                      <  2:874952/217‑1 (MQ=255)
                                                                                                  tttgtacGAAGGGGTTTTGCGCGGCGAAATGGGCCAGGGTGAGTTAAAAAATGAACCCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTc                                                                                                                                 <  2:161896/232‑1 (MQ=255)
                                                                                                     tGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCgattg                                                                                                                                                                                                                 >  1:521398/1‑156 (MQ=255)
                                                                                                     tGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGACCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCgattg                                                                                                                                                                                                                 <  2:521398/156‑1 (MQ=255)
                                                                                                       tCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGACTGATTTTGTAGGCCTGATAAGACGTGGGCCATCATGCCTCG‑GTACCAGAATGCAGGATGGGGCGGGAACCACTAATCCGGCCTaaaa                                                                                                                           >  2:384902/1‑239 (MQ=255)
                                                                                                           agagCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGGGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATa                                                                                                                        >  1:442406/1‑238 (MQ=255)
                                                                                                            gagCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTACCAGCCTGAAAGCCGCCCCCGTCGAG‑CGGCGCCCGTGGCTGGAAAGGGGGTGGCTGAAAAGCGCTTGCCTTTTGCGGCCGTGTAAACCCTAGGGCATCAAGCATCGAGCCCC‑CAACTCCGCGTGCGGCGGGACGGCCCTTTCCGTCCACCAAAAAc                                                                                                                       >  2:1144752/1‑238 (MQ=255)
                                                                                                               cTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCg                                                                                                                    <  1:633877/238‑1 (MQ=255)
                                                                                                                      gcggcgAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGGGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCa                                                                                                            <  1:384902/239‑1 (MQ=255)
                                                                                                                      gcggcgAAAGGGGCCAGGGGGGGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCa                                                                                                            <  2:854655/239‑1 (MQ=255)
                                                                                                                            aaaTGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGGGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATg                                                                                                       <  1:947460/238‑1 (MQ=255)
                                                                                                                            aaaTGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATAGCGACGATTACGCGTGGATCTAACAGCTTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATg                                                                                                       <  1:666849/238‑1 (MQ=255)
                                                                                                                               tgggCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGGGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTg                                                                                                   <  1:1026367/236‑1 (MQ=255)
                                                                                                                                  gCCAGCGTGAATTAAAAAATGAACCCGGCGCAGGCATCGGGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTgg                                                                                                  <  1:41199/237‑1 (MQ=255)
                                                                                                                                  gCCAGCGGGATCTAAATAATGACCTCGGGGGGGGGGTGGCGACGGTGCGGCGTGGATCTAGCAGGCTGAACGCCGCGCCGTTCGAG‑CTGGGCAAGTGGCTGGAAGAGGTGGAGCTGAAAAGCGATTGATTGTGTAGGCCTGATAAGACGTGGCGCATTAGTCATCGTGCACC‑GAATGCCGGATGCGGTGTGTACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTgg                                                                                                  <  2:596166/237‑1 (MQ=255)
                                                                                                                                    cAGGGTGGGTTAAAAAGAGACCGCGGGGCAGGGAGTGCGACGATGACGCGGGGATCTAACGGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCGGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAgg                                                                                              <  2:9773/239‑1 (MQ=255)
                                                                                                                                    cAGGGTGAGTTAAAAAATGAACCCGGGGCAGGCATCGCGACGATTACGGGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAgg                                                                                              <  2:442406/239‑1 (MQ=255)
                                                                                                                                     agcggtaGTTAAAAAATGAACTCGGCGCCGGCATCGGGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAgg                                                                                              <  1:204967/232‑1 (MQ=255)
                                                                                                                                       cGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAgg                                                                                                                                                                                                       >  1:997967/1‑132 (MQ=255)
                                                                                                                                       cGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGgc                                                                                                                                                                                       <  2:907757/148‑1 (MQ=255)
                                                                                                                                       cGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGgc                                                                                                                                                                                       >  1:907757/1‑148 (MQ=255)
                                                                                                                                       cGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTg                                                                                                                                                <  2:1056540/186‑1 (MQ=255)
                                                                                                                                       cGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTg                                                                                                                                                >  1:1056540/1‑186 (MQ=255)
                                                                                                                                                                 gggCTTCGCGACGATTTCGCGGGGCTCTAACAGCTTGAAAGCCGCGCCCGGCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAg                                                                 <  2:1167135/238‑1 (MQ=255)
                                                                                                                                                                    cctCGCGACGTTTCCGCTTGGAATTAACGGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAg                                                                 <  2:561980/234‑1 (MQ=255)
                                                                                                                                                                        gcgACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTTCATATGTTTGGGAGGCCTGATAAAACCCGGCCGCGTCCGATCAGGCGCTTa                                                          >  1:1071593/1‑239 (MQ=255)
                                                                                                                                                                             gaTTTCGGGTGGATCTAACAGCCTGAAAGCCGGGCCCGGCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATaca                                                     <  1:137214/239‑1 (MQ=255)
                                                                                                                                                                             gaTTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATaca                                                     <  2:430079/239‑1 (MQ=255)
                                                                                                                                                                             gaTTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATaca                                                     <  1:252742/239‑1 (MQ=255)
                                                                                                                                                                              ttttcccgtgtgtttAACAGCCTGGAAGCCGCGCCCGTCGAG‑CTGCGCCAGGGGCTGGAAGAGGTGGTGCTGAAAAGGGATTTAGTTTGTAGGCCTGATTAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATaca                                                     <  2:688866/224‑1 (MQ=39)
                                                                                                                                                                              tttccgcgGGGAGCTAAAAGCCTGAAAGCCCGGCCCGTGGAG‑CGTGGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATaca                                                     <  2:162488/234‑1 (MQ=39)
                                                                                                                                                                              agtgcccgTGGAGCTAACAGCCTTAAAGCCGGGCGCGGCGGG‑CTGCGCCAGTGGCTGGAAGAGTTGTTGATGAAAAGCGATTGAGTTTGGAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGGCGGATGCGGCGTGAACGGCTGATCCGTCCTACAAATACGCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATaca                                                     <  1:55878/232‑1 (MQ=255)
                                                                                                                                                                              aTTTCGCGGGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATaca                                                     <  1:1097890/238‑1 (MQ=255)
                                                                                                                                                                               tttgggggggATCTAACCGGCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACg                                                   <  2:1149108/231‑1 (MQ=255)
                                                                                                                                                                                     ggggATCTAACCGCCTGAAAGCCGCGCCCGTCTGG‑CTGCGCCAGTGGGTGGAAGAGTGGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCAtt                                              <  2:1177610/236‑1 (MQ=255)
                                                                                                                                                                                        gATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAAACGCGGCAGCGGCGCATCAGGGGCTTAAAAAACGGCATTTTGa                                          >  2:593941/1‑239 (MQ=255)
                                                                                                                                                                                         aTCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGGCCCACAAATACCCGCAATTTCCATATGTTTGGAAGGCATGATAAAACGCGGCAGCGTCCCATCAGGCGCATAATAAACGGCATTAAGaa                                         >  2:379394/1‑239 (MQ=39)
                                                                                                                                                                                                 gCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAAAATGTTTGGTAGGAATGGTAAAACGCGGCAGCGTCGCCTCCGGGCCTTAATAAACGGCATTAAGAAAAGGAcac                                 >  2:1252828/1‑237 (MQ=39)
                                                                                                                                                                                                   cTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCCATATGTTTGGGAGGCCTGGTAAAACGCGGCCACGTCCCCCCAGCCGCTTAATACACGCGATTTTAAAACAGAcccca                               >  2:793305/1‑235 (MQ=38)
                                                                                                                                                                                                   cTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCCCATCAGGCGCTTAATACACGGCCTTATGAAAAGGACCCa                                >  1:1242678/1‑238 (MQ=255)
                                                                                                                                                                                                             cgcCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCCACAGGCGCTTAATACACGGCTTTTTGAAACGGACCCGGGCCCAGGagc                     >  1:620461/1‑237 (MQ=39)
                                                                                                                                                                                                                  ccgcgggccgcgcCAGTGGCTGGAAGGGTTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCg                 <  2:1251689/230‑1 (MQ=255)
                                                                                                                                                                                                                    cGAG‑CTGCGCCAGGGGCTGGAAGAGGTTTTGCTGAAAAGGGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGcc               <  1:1252828/238‑1 (MQ=255)
                                                                                                                                                                                                                            cgcCAGTGGCTGGAAGAGGTGTTGCGGAAAAGCGATTGATTTTGTAGGCCTGATAAGTCGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGGCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGAt        <  2:745356/238‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                 
ACCTGCTTAAGAATGCCTACCAAAACGATCTCCATTTACCGTTGTTAAACCTGATGCTGACGCCAGATGAGCG‑CGAAGCGTTGGGGACTCGCGTGCGTATTGTCGAAGAGCTGTTGCGCGGCGAAATGAGCCAGCGTGAGTTAAAAAATGAACTCGGCGCAGGCATCGCGACGATTACGCGTGGATCTAACAGCCTGAAAGCCGCGCCCGTCGAG‑CTGCGCCAGTGGCTGGAAGAGGTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACC‑GAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGC  >  NC_000913/4632831‑4633292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A