Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F27 I0 R1 120 139.6 2835800 98.5% 2793263 226.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 732,848 T→G 13.1% V1089G (GTG→GGG)  rhsC → Rhs protein with putative toxin domain, putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913732,8480TG13.1% 211.6 / 2.9 99V1089G (GTG→GGG) rhsCRhs protein with putative toxin domain, putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base T (64/18);  new base G (3/12);  total (68/31)
Fisher's exact test for biased strand distribution p-value = 2.86e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

CGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCACGGAAGGGACAACAGCGTGGTACGCA  >  NC_000913/732610‑733086
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               
cGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAgggt                                                                                                                                                                                                                                                 >  1:1312388/1‑239 (MQ=14)
 gggACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGtgtg                                                                                                                                                                                                                                                <  1:267616/239‑1 (MQ=31)
   gACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTgtggt                                                                                                                                                                                                                                              >  1:837442/1‑239 (MQ=31)
            gcggggttgccgtttcacgggAACCCGAAATGGACCTGGTACGGGGGGAACGGGGCCCGCCTGACCACGTTACA‑GAACGAAAGACCCCGCTCCAAGACGTTTTATCGGCGGGGGAGTTTCCCGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATCCCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCccgc                                                                                                                                                                                                                                     <  2:298222/219‑1 (MQ=11)
             gtggggtgccGCTGCCACGAAAGCCGCAGGGCACGGGGTCGGGGTGGGAGGGCGACCGCCTGACCACGATACA‑GAACGACAGACCCCGCACCCAGACGATTTATCAGCGGGGGAGCTTCCCGCCCCCCATCAGGGTTGAACCC‑GCCACCGGGGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATCCCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCccgcc                                                                                                                                                                                                                                    <  2:300312/229‑1 (MQ=11)
             gCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCccgccg                                                                                                                                                                                                                                   >  1:1095736/1‑239 (MQ=31)
             gCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCccgccg                                                                                                                                                                                                                                   >  1:300312/1‑239 (MQ=31)
                ggATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGGTTTATCAGCCGGGGGGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGCTCCCCCTCAGCAGTCCGGTGGCG‑GAGACGGGGGCAGTGTGGTG‑TTCCCGCCggag                                                                                                                                                                                                                                >  2:249573/1‑237 (MQ=255)
                 ggtgccgttGTCCCGAAACCCGAAAGTGCCTGGGTCGGGCTGGCAGGGCCACCCCCTACCCACGAAACA‑GACCGACAGACCCCGCACCCAGCGGATTTACCGGCGGGGGAGCTCCCCGCCACTCGCCAGGGTTGAACCC‑GCCACCGGTGGGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGTTG‑TTCCCGCCGGTg                                                                                                                                                                                                                                <  2:906186/230‑1 (MQ=2)
                 gATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGGGTGGTG‑TTCCCGCCGGt                                                                                                                                                                                                                                 >  1:236022/1‑237 (MQ=14)
                 gATGCCCCTGCCACGGAACCCGGAGGTGCCCGGGTCCGGCTGGGACGGCGACGCCCTGACCCCGATACA‑GACCGCCAGACCCCGCATCCAGCCGTTTTACCAGCCGGGGATCTTCACGCCACTCATCAGGGTTGAACCC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTg                                                                                                                                                                                                                                <  2:1305039/238‑1 (MQ=11)
                                     cGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGCGGCAGTGAGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCg                                                                                                                                                                                                            >  1:1155324/1‑238 (MQ=14)
                                         aGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGGGCTGGTGCAGATGCTCGACCGGCTgg                                                                                                                                                                                                       >  1:1073126/1‑239 (MQ=14)
                                           tGACCGGGTACGGCGGGGACGGGGACCGCCGGCCCCCGA‑ACAGGAACGAAAGAACCCGCAGCCAGAGAATTTAGCGGCGGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCCCCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGa                                                                                                                                                                                                      <  2:552042/238‑1 (MQ=11)
                                                tGGTACGGCTGGGACGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGGGCAGATGCTCGACCGGCTGGAAAGTGa                                                                                                                                                                                                >  1:1178584/1‑239 (MQ=14)
                                                            gACGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTg                                                                                                                                                                                     >  1:1305145/1‑238 (MQ=31)
                                                            gACGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAAGGAAATCCTGGCTg                                                                                                                                                                                     >  2:14505/1‑238 (MQ=14)
                                                            gACGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGGGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTg                                                                                                                                                                                     >  2:916996/1‑238 (MQ=14)
                                                             aCGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTCCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGAG‑TTCCCGCCGCTGCTGGTGCAGATGCTCGACCGGCTGGGGAGTGGAATCCTGGCTGa                                                                                                                                                                                    >  2:948245/1‑238 (MQ=9)
                                                                           aCCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGa                                                                                                                                                                     >  1:1142614/1‑239 (MQ=31)
                                                                                gATACA‑GATCGACTTAACCCGCATCCAGACGATTTATCAGCCGTGGTGATTCCCGCCACTCCTCAGTGTTGAAACC‑GCCACCGGTGAGCTGGCGCAAACGCAGCGCCGCA‑GCCTGGCGGACACCCCTCAGCAGTCCGGTGGCG‑GAGCCGGTGGCATTGTGGTG‑TTCCCGCCGGTGCTGGTGCACATGCTCGACCGGCTGGAAAGTGAAGGCCTGGCTGCCCGGCTGAGGGAGGaaatgc                                                                                                                                                                >  2:455620/1‑236 (MQ=11)
                                                                                        aaCGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctg                                                                                                                                                         >  1:1290930/1‑239 (MQ=31)
                                                                                        aaCGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgatg                                                                                                                                                         >  1:359397/1‑239 (MQ=14)
                                                                                          cGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctg                                                                                                                                                         >  1:1052056/1‑237 (MQ=31)
                                                                                             cAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGCAAGCGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctggctg                                                                                                                                                     >  1:2008/1‑238 (MQ=14)
                                                                                             cAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCgctggctg                                                                                                                                                     >  1:607538/1‑238 (MQ=31)
                                                                                             cAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGGGAGTGAGGAAAGCCGCCgccggcgg                                                                                                                                                     >  2:136506/1‑236 (MQ=14)
                                                                                             cAGAACCAGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGCG‑TTCCCGCCGGTGCTGGTGCCGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGCGTGCGGAAAGCCGCCGctgactgg                                                                                                                                                    >  1:986431/1‑239 (MQ=9)
                                                                                               gAACCCGCCTCCAGACGATTTATCCGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCCGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGGGGCG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGCGTGAGGAAAGCCGCCGCCGGCTGGCa                                                                                                                                                  >  1:517722/1‑239 (MQ=255)
                                                                                                        tCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑CTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCgg                                                                                                                                          >  1:673840/1‑238 (MQ=14)
                                                                                                          cAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCgg                                                                                                                                          >  1:1036490/1‑236 (MQ=31)
                                                                                                          cAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCgg                                                                                                                                          >  2:868587/1‑236 (MQ=31)
                                                                                                            gACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGCTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGGGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGa                                                                                                                                     >  2:1247859/1‑239 (MQ=14)
                                                                                                                    aTCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGGGgc                                                                                                                              >  2:1081163/1‑238 (MQ=14)
                                                                                                                         ccGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGAt                                                                                                                        >  1:99533/1‑239 (MQ=32)
                                                                                                                         ccGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGAt                                                                                                                        >  1:994452/1‑239 (MQ=32)
                                                                                                                           ggggAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGc                                                                                                                      >  1:1770/1‑239 (MQ=32)
                                                                                                                             ggggcttcgcgccccacctaggggTTGAAACC‑GCCCCCGGTGAGCGGGCGAAAACGCAGCGCCGCG‑GCCTGGGGGATACCCTTCAGCAGCCGGGTGGCG‑AGGACGGTGGCGGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCa                                                                                                                     <  1:1399707/217‑1 (MQ=11)
                                                                                                                                gCTCCACGCCACCCACCAGGGTTGAACCC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGGAGCCCGGTGGCG‑AAGACGGTGGCGGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAg                                                                                                                  <  1:7854/238‑1 (MQ=255)
                                                                                                                                          ccccagtcgggTTTAAACCGGCCCCCGTGGAGCGGGAGAAAGCGCGGCGCCGCA‑GCGGGGCGGATCCCCTCCAGTGGCCCGGGGGGG‑AAGCGGGTGGCGGGTTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGAc                                                                                                        <  2:1178584/231‑1 (MQ=11)
                                                                                                                                          aCTCATCAGGGTGGAACCC‑GCCCCCGGTGAGCTGGCGAAACCGCAGCGCCGCA‑GCCTGGCGGATCCCCTTCAGCAGTCCGGGGGGG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGAcc                                                                                                       <  1:140850/239‑1 (MQ=255)
                                                                                                                                           cccacaaggtgtaaatcc‑gccgGCGGGGGGCGGGCGAAACCCCGGCCCCGCA‑GCCTGGGGTATCCCTTTCAGCGGTCGGGGGGGA‑AGGCCGGTGGGAGTGGGGTG‑TCCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGAccc                                                                                                      <  2:1073126/223‑1 (MQ=11)
                                                                                                                                           cTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCCGATGCAAAGCCAGATGGAccc                                                                                                      >  2:264452/1‑239 (MQ=18)
                                                                                                                                              atcaGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGt                                                                                                   >  1:43846/1‑239 (MQ=33)
                                                                                                                                                   ggTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGCGGCGCAGATGCAACGCCAGATGGACCCGGTATac                                                                                               >  1:412663/1‑238 (MQ=25)
                                                                                                                                                   ggTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGGGAGTGAGGAAAGCCGCCGCTGGATGGCATCGGGCGGCCTGACGGTTGCGCAGATGCCAAGCCCGGAGGACCCgggttac                                                                                               >  2:83801/1‑233 (MQ=11)
                                                                                                                                                      tGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACg                                                                                            >  2:233758/1‑238 (MQ=35)
                                                                                                                                                      tGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACg                                                                                            >  2:835634/1‑238 (MQ=35)
                                                                                                                                                        aaaCC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCg                                                                                         >  1:120079/1‑239 (MQ=35)
                                                                                                                                                              gccgcgggggAGCTGGCGAAAACGCAGCGCCGGA‑GCCGGGCGGATACCCTTCAGCGGTCCGGGGGGA‑AAGAGGGTGGCGGGGTGGTG‑TCCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGcgcg                                                                                    <  2:631413/230‑1 (MQ=11)
                                                                                                                                                              gCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGcgcg                                                                                    >  2:847581/1‑239 (MQ=35)
                                                                                                                                                              gCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGcgcg                                                                                    >  1:644479/1‑239 (MQ=35)
                                                                                                                                                              gCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGGGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGCTGGACCCGGTATACACGCCGGcgcg                                                                                    >  2:731725/1‑239 (MQ=25)
                                                                                                                                                              gCCACCGGGGAGCGGGAGAAACCGAGGCGCCGCG‑GCCGGGCGGAGACCCTTAGGCAGGCGGGGGGCG‑AAGGCGGGGGCAGTGGGGTG‑TTCCCGCCGGTGCGGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGcgcg                                                                                    <  1:1090233/239‑1 (MQ=11)
                                                                                                                                                               ccACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGa                                                                                   >  1:808743/1‑239 (MQ=35)
                                                                                                                                                                cACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTg                                                                                                                                             >  1:403570/1‑180 (MQ=31)
                                                                                                                                                                   cggggacgtGGCAAAAACGAGGCGCCGCA‑GCCGGGGGGATCCCCTTCAGCAGTCCGGGGGCG‑AGGGCGGTGGCAGTGGGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGaaaaa                                                                               <  1:832037/231‑1 (MQ=11)
                                                                                                                                                                       gggggggCAAAAACGCAGCG‑CGCAGGCCGGGGGTATACCCTTCAGAGGGCGGGTGGGG‑AAGGCGGGGGCAGGGGGGTT‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTc                                                                            <  2:1036490/233‑1 (MQ=11)
                                                                                                                                                                       ggggggccaaaaaCGGGGGGCGGCA‑CCCTGGGGGAT‑CCCTTCAAGAGGCCGGGGGGGAAAGCCGGTGGGAGTGGGGTG‑TCCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTc                                                                            <  2:607538/229‑1 (MQ=11)
                                                                                                                                                                       gagggggggAAAACGCGGCGGCGCA‑GCGTGGCGGACCCCCTGCAGCACGGCGGTGGCG‑AAGAGGGTGGCGGTGGGGGT‑TCCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTc                                                                            <  2:1770/230‑1 (MQ=11)
                                                                                                                                                                       gAGCGGGCAAAAACGCAGCGCCGCA‑GCCTGGCGTATCCCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTc                                                                            <  1:37137/238‑1 (MQ=255)
                                                                                                                                                                         gctgcggAAAACGGAGCGCCGCA‑GCGGGGCGGATCCCCTTCGGCAGGCCGGGGGCG‑AAGACGGGGGCAGTGTGTTG‑TCCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCGGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCAc                                                                          <  2:837442/232‑1 (MQ=11)
                                                                                                                                                                         gCTGGCGAAACCGCGGCGCCGCA‑GCCTGGGGGATCCCCTCCAGCAGTCGGGTGCCG‑AAGACGGGGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCAc                                                                          <  1:455066/238‑1 (MQ=11)
                                                                                                                                                                         gCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCAc                                                                          <  1:835634/238‑1 (MQ=37)
                                                                                                                                                                           tGGCGAAAACGCAGCGCCGCA‑GCTTGGCGGATACCCTGCAGCAGTCCGGTGGCG‑AAGACGGGGGCAGTGGGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTg                                                                       <  1:1080011/239‑1 (MQ=255)
                                                                                                                                                                              cGAAACCGCAGCGCCGCA‑GCCGGGGGGATCCCCTCCAGCAGTCGGGGGGCG‑AAGCCGGTGGCAGTGGGGTG‑TCCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTa                                                                     <  1:249573/238‑1 (MQ=11)
                                                                                                                                                                                            cgcA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCCCCTGTACCACTGCGCCCATc                                                       >  2:646904/1‑238 (MQ=37)
                                                                                                                                                                                                         gATCCCCTCCGGCAGTCGGGGGGAG‑AAGACGGGGGAGGTGGGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCGCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGc                                           <  1:1194731/238‑1 (MQ=11)
                                                                                                                                                                                                           tACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTg                                         >  1:518941/1‑238 (MQ=37)
                                                                                                                                                                                                            aCCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTgg                                        >  2:1268970/1‑238 (MQ=37)
                                                                                                                                                                                                            aCCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTgg                                        >  2:44075/1‑238 (MQ=37)
                                                                                                                                                                                                            aCCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTgg                                        >  1:360847/1‑238 (MQ=37)
                                                                                                                                                                                                            aCCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGGGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCCAAGCCAGATGGACCCGGTATACACGCCGGCGCGACAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTgg                                        >  1:572862/1‑238 (MQ=255)
                                                                                                                                                                                                                ttCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCtt                                   >  1:678615/1‑239 (MQ=38)
                                                                                                                                                                                                                ttCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCtt                                   >  1:860554/1‑239 (MQ=38)
                                                                                                                                                                                                                ttCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTACACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCCGGCCCtt                                   >  2:1236651/1‑239 (MQ=255)
                                                                                                                                                                                                                     cagTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCa                               >  1:813004/1‑238 (MQ=38)
                                                                                                                                                                                                                        tCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCa                            <  1:1268970/238‑1 (MQ=38)
                                                                                                                                                                                                                        tCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAc                           >  1:577891/1‑239 (MQ=39)
                                                                                                                                                                                                                        tCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAc                           >  2:595753/1‑239 (MQ=39)
                                                                                                                                                                                                                        tCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAc                           >  1:331991/1‑239 (MQ=39)
                                                                                                                                                                                                                        tCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAc                           >  1:847781/1‑239 (MQ=39)
                                                                                                                                                                                                                        tCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAc                           >  1:1397650/1‑239 (MQ=39)
                                                                                                                                                                                                                        tCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAc                           >  1:381663/1‑239 (MQ=39)
                                                                                                                                                                                                                        gcggggggCG‑AGGAGGGTGGCGGGGTGGTG‑CACCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCa                            <  2:673840/232‑1 (MQ=11)
                                                                                                                                                                                                                         ccGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTACCCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTACTAGCACg                          >  2:251809/1‑239 (MQ=255)
                                                                                                                                                                                                                          cGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGCCCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGGATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATGGCGGCCTGGCGCCGGCCCTTAtggccacgg                         >  2:897044/1‑231 (MQ=255)
                                                                                                                                                                                                                          cGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCACgg                         >  2:52046/1‑239 (MQ=40)
                                                                                                                                                                                                                          cGGTGGCG‑AAGACGGTGGCAGTGTGGAG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCACgg                         >  1:736654/1‑239 (MQ=255)
                                                                                                                                                                                                                                   aaGGCGGGGGCAGTGGGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCACGGAAGGGac                  <  1:128027/238‑1 (MQ=255)
                                                                                                                                                                                                                                   aaGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGCTGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCACGGAAGGGaca                 >  1:395746/1‑239 (MQ=38)
                                                                                                                                                                                                                                       gggTGGCGGTG‑GGTGTTTCCCGCCGTTGCTGGTGCAGATGCCCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCACGGAAGGGacaaca              <  2:604672/237‑1 (MQ=255)
                                                                                                                                                                                                                                       acagggCGGTTTGGTG‑TTCCCGCGGGTGCGGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCGGGCTGACCGGGTAAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGTCTGGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCACGGAAGGGacaaca              <  1:850088/234‑1 (MQ=11)
                                                                                                                                                                                                                                            gaggtgtGGTT‑CTCCCGCCGGGGCGGGGGCAGATGCCCGCCCGGCGGGAAAGTGAAATCCGGGCTGACCGGGGGGGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCCCCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCACGGAAGGGACAACAGCGTg         <  2:840494/235‑1 (MQ=14)
                                                                                                                                                                                                                                            gCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCACGGAAGGGACAACAGCGTgg        >  1:840494/1‑239 (MQ=40)
                                                                                                                                                                                                                                                tgtgGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCACGGa                        <  1:404361/219‑1 (MQ=39)
                                                                                                                                                                                                                                                tgtgGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCACGGa                        >  2:404361/1‑219 (MQ=39)
                                                                                                                                                                                                                                                 ttggtt‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCACGGAAGGGACAACAGCGTGGTACg    <  1:775185/237‑1 (MQ=255)
                                                                                                                                                                                                                                                  tggtg‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCCGCACGGAAGGGACAACAGCGTGGGACGcc  >  2:156489/1‑238 (MQ=255)
                                                                                                                                                                                                                                                  tggtg‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCCCGGCCTGCCGCTGGCCCTTATCAGCCCGGAAGGGACCACAGCGGGGTACGCa  >  2:1416146/1‑239 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               
CGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGCCTGACCACGATACA‑GAACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACC‑GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCA‑GCCTGGCGGATACCCTTCAGCAGTCCGGTGGCG‑AAGACGGTGGCAGTGTGGTG‑TTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAAGCCAGATGGACCCGGTATACACGCCGGCGCGAAAAATTCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCACGGAAGGGACAACAGCGTGGTACGCA  >  NC_000913/732610‑733086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A