Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F17 I0 R1	155	84.1	1797446	90.9%	1633878	230.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	742,267	T→C	14.4%	E97G (GAG→GGG)	dtpD ←	dipeptide and tripeptide permease D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	742,267	0	T	C	14.4%	163.7 / 3.7	73	E97G (GAG→GGG)	dtpD	dipeptide and tripeptide permease D
Reads supporting (aligned to +/- strand): ref base T (41/20); new base C (2/10); total (43/30)
Fisher's exact test for biased strand distribution p-value = 2.44e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAG‑ATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCC  >  NC_000913/742037‑742497

ccAGGCCAACGCCCAGCGCCCAGCTGTACTCTCCCTGGGCGAACCCACAGGCGATAGGTGCAATAATAGCCCCCACGTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCa                                                                                                                                                                                                                                   <  2:660268/239‑1 (MQ=255)

      cAAAGCCCAGCGCCCAGCTGTCCTCTCCCGGGGCGTAACCACAGCAGAAAGGTGCAATAATAGCCCCCCCGTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAg                                                                                                                                                                                                                              <  2:576308/238‑1 (MQ=255)

      cAAAGCCCACCGCCCCGCTGTACTCTCCCTGGGCGTAACCACAGGGAAAGGGGGCGAAAATAGCCCCCACGTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAg                                                                                                                                                                                                                              <  2:813816/238‑1 (MQ=255)

      aaaaGCCCACCGCCCGGCTGTACTCCTCCTGGGCATAACCGAAGGGGGTGGGGGCAATAATACCCCCCACTTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAg                                                                                                                                                                                                                              <  2:163537/237‑1 (MQ=255)

                     aGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCCCCCGGGGCAGATAAAGGAATGACGGATGGATCTCAATGGCACCCAGcaccaacacaccgaa                                                                                                                                                                                                               >  1:794040/1‑228 (MQ=255)

                          tACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCAt                                                                                                                                                                                                        >  2:775241/1‑239 (MQ=255)

                          ccccctcccggggcaaaacccagggCGATAGGTCGAATAATAGACCCACCTTTACCCGCCGCAAACATCCGCGAAAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCAt                                                                                                                                                                                                        <  2:723012/216‑1 (MQ=255)

                                 ccTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATcacc‑aa                                                                                                                                                                                                 >  2:761587/1‑238 (MQ=255)

                                 ccTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGACACCCCGAACCACA‑AGACCGATGGACATCaaa‑aac                                                                                                                                                                                                >  1:363065/1‑239 (MQ=255)

                                 ccTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAAAGGAATGACGGCTGGATCCCACTGGACCCCCACACCACA‑TAACCGACGGCCACcaaa‑aaa                                                                                                                                                                                                >  1:276949/1‑238 (MQ=255)

                                       cGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATATAGGAATAACGGATGGATCTCACTGGCACCCATCACCAAA‑TGACCGAACGCCATCAAA‑AACGccccc                                                                                                                                                                                          >  2:370558/1‑239 (MQ=255)

                                         tAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGCCAGCTTACGGTAGATTTAAACAGGCCATAGCCGCAGACGACAACCGCCAGGGACAGATACAGGAATGCCGGATGGATTCCCATGGCACCCAGCACCACA‑TGACGGATCGCCATCAAA‑AACCCCCCCac                                                                                                                                                                                        >  1:194195/1‑238 (MQ=255)

                                           aCCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGGTCTCACTAGCACCCAACCCCACA‑TAACCGCACACAATCAAC‑AACGcaccccaca                                                                                                                                                                                      >  1:81162/1‑235 (MQ=255)

                                                       aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCATGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGCCGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑AGACCGATCGCCATCAAA‑CACGCCCCCCGCCTCACCCCCacgg                                                                                                                                                                          >  1:629107/1‑236 (MQ=255)

                                                       aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATGGCCAGGGACAGATAGCGGAATGACGGATGGATCTCACTGGGACCCA‑CGCCACACTGACCGATCGCCACAAAA‑AAACCCCCAAACAACACCGcacagg                                                                                                                                                                          >  1:14389/1‑234 (MQ=255)

                                                       aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAA‑AACGCCCCCAGCATCACCGCCATGc                                                                                                                                                                          >  2:705835/1‑239 (MQ=255)

                                                            cGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAAACGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACAACA‑TAACGCATCGCCA‑CAACAAACGCCCACAGCACCACCCCCAAGcagagc                                                                                                                                                                     >  1:436068/1‑234 (MQ=255)

                                                                   aGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGCATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑ACGGCCCCCAGCATCACCGCCATGCGATTGACgcga                                                                                                                                                               >  2:286747/1‑238 (MQ=255)

                                                                    gACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCCGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTAACTGGCACCCAGCCCCACA‑AGACCGATCGCCATCAAC‑AACCCCCCCAGCACCACCCCCaaccacataccgcaa                                                                                                                                                               >  1:83030/1‑223 (MQ=255)

                                                                         cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGGTGGATCTCACTTGCCCCCAGCACCCCA‑GGCCCGATCGCCATCACA‑AAACCCCCCCGCATCACCGCCATGCGAAGGCCGAGAACATTa                                                                                                                                                         >  1:454902/1‑238 (MQ=255)

                                                                         cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCCCCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCCACCCCATGCGAATCCCGGAGACTTTac                                                                                                                                                        >  1:284214/1‑238 (MQ=255)

                                                                         cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCACCACCGCCATGCGATTGCCGAGACCTTTa                                                                                                                                                         >  2:205548/1‑238 (MQ=255)

                                                                         cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCCCCCTCAAC‑AACGCCCCCACCATCACCCCCAGGCGGTTGCCGGAAACttaa                                                                                                                                                         >  1:487199/1‑236 (MQ=255)

                                                                         cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACAACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGAATCACCGCCATGCGATTGCCGAGAACTTTa                                                                                                                                                         >  2:204520/1‑238 (MQ=255)

                                                                         cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGGTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGCCAGATAAAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGGTCGCCATCAAC‑AACCCCCCCCGCATCCCCGcacaacaccttccggaaacttta                                                                                                                                                         >  1:543884/1‑216 (MQ=255)

                                                                         cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCCCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACCGCTAGAGGAATGCCGGATGGATCTCACTTGCACCCAGCACCACA‑TGACCGATCGCCAGCAAC‑AACCCCCCCAGCATCACCGCCACGCGCTTCCCGAGAACtttc                                                                                                                                                         >  1:670098/1‑237 (MQ=255)

                                                                           cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCCGCATCACCGCCATGCGATTGCCGAGAACTTTATc                                                                                                                                                       >  2:304475/1‑238 (MQ=255)

                                                                               acccgcccgaataatccgggagaACCCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTGGCCGAGCAGGCAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCa                                                                                                                                                  <  2:263041/220‑1 (MQ=255)

                                                                                   gccgcATACATCAGCGAGAAACCGCCATCACGAAGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTAACCGCCaaaaa                                                                                                                                              >  2:725864/1‑239 (MQ=255)

                                                                                     cgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGCCGATAATCGCCAGGGACAGATAGAGGAAGGACGGATGGATCTCCATGGCACCCATCACCACA‑TGACCGAACGCCCTCAAC‑AACGCCCCCAGCCTCCCCGCCATACGACTGACGAGAACTTTACCCCCCaaaaaa                                                                                                                                             >  1:853565/1‑238 (MQ=255)

                                                                                     cgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCCCA‑TGACCGAACGCCCTCAAC‑AACGCCCCCAGCATCACCCCACTCCCATTACCCAGCACTTTATCCACCAAAAAg                                                                                                                                             >  1:110148/1‑238 (MQ=255)

                                                                                         tACATCGGCGAAAAACCGCCATCACGGCGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCAcc                                                                                                                                        <  2:487199/239‑1 (MQ=255)

                                                                                               agggagaACCCGCCATCACGACGCGGATCGGTT‑GGCTAATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGAt                                                                                                                                  <  2:194195/236‑1 (MQ=255)

                                                                                                 cGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATCCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTg                                                                                                                                >  2:16805/1‑239 (MQ=255)

                                                                                                  gaaaaaCCGCCATCCCGACGGGGATCGGTT‑GGCTAATCAAGCTCGCCGAGCAGACAGTTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGa                                                                                                                                   <  2:794040/232‑1 (MQ=255)

                                                                                                   agaAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGACCTTTATCGGCCAAAAAACCACAGAGGATTGGc                                                                                                                              >  2:829573/1‑239 (MQ=255)

                                                                                                    aaaaCCGCCAACACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCg                                                                                                                             <  2:276949/238‑1 (MQ=255)

                                                                                                     aaaCCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCCAC‑AACGCCCCCAGCATCCCCGCCATGCGATTGCCGGGCACTTTATCCGCCAAAAAGCAACCGAAGAAGGGCgg                                                                                                                            >  1:54242/1‑238 (MQ=255)

                                                                                                      aaCCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATCCGATTTCCGGGAACTTTATCCGCCAAAAAAACACCCAGGATTGGccgg                                                                                                                           >  1:98849/1‑236 (MQ=255)

                                                                                                              tCACGACGGGGATCGGTT‑GGCTCATACAGCTCCCCGAGCAGACAGCTTACGTTAGATTTAACCAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATacac                                                                                                                   <  2:584125/239‑1 (MQ=255)

                                                                                                                   acgCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGGGAAATTTATCCGCCAAAAAGCCACCGGGGATTGGGGTGAAATAAACCAGcc                                                                                                              >  1:798646/1‑238 (MQ=255)

                                                                                                                    cggggATCGGTT‑GGCTCATCAAGCTCGCCGGGCAGACAGCTTCCGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCg                                                                                                              <  2:284214/235‑1 (MQ=255)

                                                                                                                                    tCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCCATTGCCGGGAACTTTATCCGCCAAAAAGCCACCCAGGATTGGCGGGACATACACCCGCCaaaaaaaggggcaaaa                                                                                              >  1:10221/1‑225 (MQ=255)

                                                                                                                                           cccccccGAGCAAAAAGTTGATGTAAGATTAAAACAGCCCAGTGCCCGAAAGAAGAACCCCCAGGGACAGAAAGAGGAAGGACGGATTGACCTCACTGGCACCAAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCg                                                                                       <  1:705835/234‑1 (MQ=255)

                                                                                                                                             tCGCCGGCCAGACAGCTTAGGTTAGATTTAAACAGGCCATAGCCGCAGACGATAAGCGCCAGGGACAGATAGAGGAATGACGGATGGATCCCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCg                                                                                       <  2:555177/237‑1 (MQ=255)

                                                                                                                                                     cagcaagtctagttaaaaTTAAAACAGGCACACGCCGGAGACGATAACCCCAAGGGACAGAAAGAGGAATGCGGGATGGCCCCCACGGCCCCCCCGCACCACA‑AGCCCGGTGGCCATCAAA‑AACGCCCCCAGCATCTCCGCCATGCGATGGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAg                                                                             <  1:73274/222‑1 (MQ=255)

                                                                                                                                                      aGACATCTTACGTTAGATTTAAACAGGCCATGGCCGGAGACGATAACCGCCAGGGAAAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAg                                                                             <  2:588767/238‑1 (MQ=255)

                                                                                                                                                       aacatttttcGTTAGTTTTAAACGGGCCAAGGCCCCACACGATAAACGCCAGGGAACGATAGAGAAATGACGGATGGATCCACCTGGCACCCAGCACCACA‑TGACCGACCGCCATAAAC‑ACCGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTa                                                                           <  1:16805/230‑1 (MQ=255)

                                                                                                                                                         agtcTTGCGTTGGATTTAAACAGGCCATGCCGGCAGACTATAATCGACAGGGGCAGATGGGGGAAGGACGGATGGATCTCCCTGGCACCCAGCACCACA‑TGCCCGATCGCCATCAAC‑ACCGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTAt                                                                          <  1:370558/235‑1 (MQ=255)

                                                                                                                                                         aacgttACTTTAAATTTAAACAGGCCATGGCCGCAAACAATAATCGCCAGGGACAGATAGGGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTa                                                                           <  2:794599/233‑1 (MQ=255)

                                                                                                                                                           agaagcaaaaatttaaaaCAGGCCAAGCCCGCAACCCAAAACCCCCAGGGAACAATAAGGGAATGCAGGAAGGACCCCCCTGGCCCCACGCCCCAAA‑GGCCCGATCGCAATAAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGt                                                                       <  1:881711/224‑1 (MQ=255)

                                                                                                                                                             ttACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACGGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCg                                                                        <  2:480949/236‑1 (MQ=255)

                                                                                                                                                             atACGTTAGGTTTAAACAGCCAGTAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGCCGGATGGATCTCACTGGCCCCCAGCACCAAA‑TGACCGATCGCCTTCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCg                                                                        <  2:697760/235‑1 (MQ=255)

                                                                                                                                                                   taaaTTTAAACGGCCCATAGCCACAGGCGATAATCCCCAGGAACAGAAAGAGGAAGGAAGGGTGGATCTCACTGGCACCCACCCCCACA‑GGACCGATGGCAACAAAC‑AACGCCCCAAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTAttt                                                                <  1:254710/235‑1 (MQ=255)

                                                                                                                                                                    tgttttAAACAGGCCCTTGCCCCAAACGTTAACCGCCGGGGAAAGAGAGAGGAATGCCGGATGCATCCCACTGGCCCCCGGCACCCAA‑TGACCGATCGCCATCAAC‑ACCGCCCCCAGCATCCCCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTa                                                              <  1:761587/236‑1 (MQ=255)

                                                                                                                                                                          aacagggCCATAGCCCAAGACGAAAACCCCCAGGGCCAGAAAGGGGAATGACGGATGGATCCCCCTGGAACCCACACCCAAA‑TGCCCGACCCCCATCAAC‑AACCCCCCCAGCATCCCCGCCATGCGATGGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGAt                                                        <  1:204520/234‑1 (MQ=255)

                                                                                                                                                                            acgggCCATAGCCGAAGGCGATAAGCGCCAGGGACAGATAGGGGAATGACGGATGGATCTCACTGGCACCCAGCCCCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTg                                                      <  2:310674/236‑1 (MQ=255)

                                                                                                                                                                              aGGCCATAGCCGCAGACGATAACCGCCAGGGACAGATAGAGGAATGACGGATGGATCCCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCAAGCATCACCGCCATGCGATTGCCGGGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGt                                                    <  2:10221/239‑1 (MQ=255)

                                                                                                                                                                               ggCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAg                                                             >  2:881711/1‑229 (MQ=255)

                                                                                                                                                                                     aGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACCCCAGCGAACAGTAGGCGCAAAATAACACGTAGGCGTGGGTATCGGTGTATTTTAGTTGATTGgagag‑aaaa                                            >  1:310674/1‑236 (MQ=255)

                                                                                                                                                                                          cAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCCCCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTAATTGGGGAG‑AAAAAGaaa                                       >  1:432441/1‑238 (MQ=255)

                                                                                                                                                                                          cAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCCCCGAGGATTGCGGGGACATACACCAGCGAAACGTAGCCGCTAAATAACTAGTAGGCGGGAGAATCGTTTTATTTTATTGGGTTGGGAAA‑AAAGAGaaa                                       >  1:854028/1‑238 (MQ=255)

                                                                                                                                                                                          cAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGCAGGCGTGAGAATCGTTGTATTTTAGTTGATTGGTGGG‑ATAGAGAAt                                       >  2:123638/1‑239 (MQ=255)

                                                                                                                                                                                              agaaaaTCCCCAGGGCAAGATAGAGGAATGCCGGATGGATCCCACTGGCCCCCAGCACCAAA‑TGACCGATCGCCATCAAC‑AACGCCCCAAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAG‑ATAGAGAATcag                                    <  2:432441/234‑1 (MQ=255)

                                                                                                                                                                                                  aagcGCCAGGGACAGATAGGGGAATGGCGGATGGATCCCAGTGGCACCCAGCCCCCCA‑TGACCGATCGCCTACAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAG‑ATAGAGAATCAGCAggg                               <  2:110148/236‑1 (MQ=255)

                                                                                                                                                                                                         aGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAAGCGCCAAATAACACGAAGGGGGGGGTACCGTTGTATTTTAGTTTATTGGTGAG‑CAAGAAAATACAAAGGGCACgaa                         >  1:311728/1‑236 (MQ=255)

                                                                                                                                                                                                           ggACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGGGAG‑ATAGAGAATCAGCAGGGCACGCATg                       >  2:79884/1‑238 (MQ=255)

                                                                                                                                                                                                               aattgggggAATGAAGGTTGGACCCCATGGGCACCCAGCACCACT‑TGCACGATCGCCATCAAC‑AACGCCCACAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAG‑ATAGAGAATCAGCAGGGCACGCATGCCGTa                  <  2:336101/232‑1 (MQ=255)

                                                                                                                                                                                                                     agGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGACCAGTATGCGCTAAATAACTCACAGTAGTGAGTATCGTTGTATTTTAGATGAGTGGAGAG‑ATAGAGAATCAGCCGGGCCCGCATACCGTAAAAGca            >  1:123165/1‑238 (MQ=255)

                                                                                                                                                                                                                     agGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAG‑ATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCt            >  2:262410/1‑239 (MQ=255)

                                                                                                                                                                                                                       gAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGCCGTGACATACACCAGCGAACAGTACGCGCTAAATACCTCGTAGGCGCGAGTACCGTTGCATTTTAGTTGATTGGTGAG‑ATAGAGAATCACCAGGGCACGCATGCCGTaaaacttaa          >  1:158948/1‑234 (MQ=255)

                                                                                                                                                                                                                             cGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAG‑ATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAaaat     >  2:96285/1‑238 (MQ=255)

                                                                                                                                                                                                                               gatggatCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATAAACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTTGATTTTAGTTGTTTGG‑GAGAATAGGGAATCAGCAGGGCCCCCATGCCGAAAAAATAAAAAAATTcc  >  1:67699/1‑239 (MQ=255)

CCAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAG‑ATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCC  >  NC_000913/742037‑742497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A