Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F9 I0 R1 71 67.1 1514203 90.7% 1373382 220.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,235,396 T→G 21.7% V45G (GTG→GGG)  preA → dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,235,3960TG21.7% 78.8 / 3.4 57V45G (GTG→GGG) preAdihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
Reads supporting (aligned to +/- strand):  ref base T (19/21);  new base G (13/2);  total (34/23)
Fisher's exact test for biased strand distribution p-value = 1.30e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑CTTCGA‑TCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGC  >  NC_000913/2235180‑2235623
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         
ggTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGGAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCCACTGCTATTGGATGGGTGCCAAAGCCTACGACACCGGTT‑GGGGCGGGTGGGAGTTTTAAACGAt                                                                                                                                                                                                                               >  1:127864/1‑229 (MQ=255)
ggTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTTTGCCTCTCTTCTTCGCCGGTAGGCCACTGCTATGAGTTGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGGGTGTGGTTTTAAACGag                                                                                                                                                                                                                               >  1:489006/1‑228 (MQ=255)
ggTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGGAACTGCTATGAGATGTGTGCCACAGCCTACGAACCCGGTT‑TGGGCGGGGGGGGGTTTAAAAAGaa                                                                                                                                                                                                                               >  2:717636/1‑228 (MQ=255)
ggTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACGATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAGGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCATTTAGATGTTTGCCAAACCCTACGACCCGGGTTGGGGGGGGTGGGGTGTTAAAAAcaa                                                                                                                                                                                                                                >  2:104239/1‑227 (MQ=255)
   gACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTCTGAGATGTGTGCCAAAGCCTACGAACCAGGTT‑GGGGCGGGGTGGGGGTTAAAACGATCgg                                                                                                                                                                                                                            >  2:25060/1‑229 (MQ=255)
     aaaaaCCGTGGTCGAGGCCGTGAAACCCGGGAAAGAAGCCGCCGAGGCGATTCCTCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCt                                                                                                                                                                                                                          <  1:589793/228‑1 (MQ=255)
             tGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCCATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGGGGGGTTTAAAACGAACGGCTTTTTTAtt                                                                                                                                                                                                                  >  2:444872/1‑228 (MQ=255)
              ggTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTTAAACGATCGGCTTTTTTATc                                                                                                                                                                                                                  >  1:706372/1‑228 (MQ=255)
                    tGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGCGGGTTTGGTGTTTAAAACGATCGGCTTTTTTTTCGCCAAc                                                                                                                                                                                                            >  1:561385/1‑228 (MQ=255)
                         tGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTATAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCTCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGCCACAGGGT‑GGGGCGGGGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGC‑AGtc                                                                                                                                                                                                     >  1:198931/1‑229 (MQ=255)
                         tGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAACGATCTTTCGCTTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTATTCGCCGGTCGGCAACTGCTATGAGATGGTTGCCCAAGCCTACAGATCAGGTT‑GGGGAGGGTGGGTGTTTAAACCAACAGGCTTCTTTTTTCCCAACaa‑aacc                                                                                                                                                                                                     >  2:238523/1‑226 (MQ=255)
                         tGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACCCAGGTT‑GGGGCGGCGGGGGGTTTTAAACGATTGGCTTTTTTTTTGCCCACGG‑Aggc                                                                                                                                                                                                     >  1:20728/1‑227 (MQ=255)
                              aCCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCCGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTTTGCCAAAACCTACGACACAGGTT‑GGGGCGGGGGGGGGTTTAAAACGATCGGCTTTTTTTACGCCAAAGAGAGtctccg                                                                                                                                                                                                 >  2:670947/1‑227 (MQ=255)
                                 gggAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCcgcg                                                                                                                                                                                             <  1:688115/229‑1 (MQ=255)
                                 gggAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTACCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTCCGACACAGGTT‑GGGGCGGGGTGGAGGTTAAAACGATCGGCTTTTTTATTGCCAACGa‑aattttgccccc                                                                                                                                                                                             >  1:640315/1‑218 (MQ=255)
                                           ccgccgAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGatcat                                                                                                                                                                                   <  1:379152/229‑1 (MQ=255)
                                             gccgAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGAAACAGGTT‑GGGGCGGGGTGGGGGTTTAAACGGTCGGGTTTTTTTTCTCCAAAGA‑AGTGACGCCGGGTTTTggataatc                                                                                                                                                                                 >  2:503481/1‑222 (MQ=255)
                                                                     gagggaggTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTAt                                                                                                                                                         <  1:509949/229‑1 (MQ=255)
                                                                         gAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACGCAGGTT‑GGGGCGGTGTGGGGTTTTAAACGGATGGCTTTTTTTTCGCCAAAGG‑AGGCTGGCCGCGTTTTGTTACTCTTGGGTACGAAAGTACCGGTTTTTTTGGc                                                                                                                                                     >  1:408629/1‑229 (MQ=255)
                                                                         gAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGCGTGGGGTTTTAAACGATCGGCTTTTTTATTGCCAACGG‑AGGCTCACCGCGTTTTTATCATCTGGTGAAAAAAAAAACAGGTTTTAACggg                                                                                                                                                     >  2:258680/1‑228 (MQ=255)
                                                                           gCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGCTGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGGGTGGGGTTTTAAACAGTCGCCCTTTTTTTCGCCAAAGC‑AAGCTCCCCCGGTTTTTATCATCTGGTGGAAGAAGTAACCGGgttttacggtgt                                                                                                                                                   >  2:534669/1‑221 (MQ=255)
                                                                           gCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTATAACGATTCGCCTTTTTTTCGCCAAAGA‑AGTCTTGCCGGGTTTTGATCATCTGGTGAAAGAAGATACCGGattttttggact                                                                                                                                                   >  1:674388/1‑221 (MQ=255)
                                                                           gCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGGCTCGCCGCGTTTTTGTCATCTGGGGAAAGAAGATACCGGgttttttcgctt                                                                                                                                                   >  1:19690/1‑221 (MQ=255)
                                                                           gCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGGT‑GGGGCGGTGTGGGGTTTAAAACGATTGGCTTTTTTATCTCCAACGA‑AGTGTTTGCGGGGTTTGGTCACCTGGGGAAAGAAAAAACAGGGTTTTTccggtg                                                                                                                                                   >  2:235984/1‑224 (MQ=255)
                                                                           gCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGGAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGGGTGGTTTAAAACGATCGGCTTTTTTTTCGCCCACGA‑AGTCTCCCCGCGTTTTTATCACCTGGGGAAAAAAGAGACAGGgtttttacgctt                                                                                                                                                   >  2:383900/1‑221 (MQ=255)
                                                                                          cGAAAGATCTTTCGATTACTTTGTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCa                                                                                                                                   <  1:258680/229‑1 (MQ=255)
                                                                                               gATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGGGTGGGGTTTAAAACGATAGGCTTTTTTATCGCCAACGA‑AAGTTCGCCGCGGTTTTATCATTTGGGGAAAAAAGGTACCGGGTTTTTTGGCTT‑CTAAAAAATAGAGCaagaat                                                                                                                              >  2:252882/1‑224 (MQ=255)
                                                                                               gATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGGTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGGCACAGGTT‑GGGGCGGGTTGGTGTTTAAAACGATCCGCTTTTTTATCCCCAACGA‑AGGCTTGCCTCCTTTTTGTTATCTTGTGACGAAAAAAACCGGGTTTTTCCGCTT‑GAAAAataatggggagaat                                                                                                                               >  2:413884/1‑217 (MQ=255)
                                                                                               atttttgtgttttgtttttGCGGAGTGGAGTTTCCCAACCCGTTCTGCCGCGCTTCTGCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTg                                                                                                                              <  2:20728/215‑1 (MQ=255)
                                                                                                    ttCGATTACTTTTGGCGGCGTGAAGTTTCCCAACCCTTTCTGCCTCTCTTCGTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGaa                                                                                                                         <  1:717637/229‑1 (MQ=255)
                                                                                                      cGATTTTTTTTTGGGGCTGGAATTTTCCCAACCCGTTCGGCCTCTCTTCTGCGCCGGTAGGCAACTGCTATGAGATGTTTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACa                                                                                                                       <  2:561384/229‑1 (MQ=255)
                                                                                                           aCTTTTTGCGGCGTGAATTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATTTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTTTGGGGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGt                                                                                                                  <  2:557010/229‑1 (MQ=255)
                                                                                                           aCTTTTGGGGGCGTGAAGTTTCCCAACCCGTTCTGCCTGTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACTCAGGTT‑GGGGCGGTGTGGTGTGTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGt                                                                                                                  <  1:173075/229‑1 (MQ=255)
                                                                                                                                        gTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCgg                                                                                     <  1:444872/229‑1 (MQ=255)
                                                                                                                                        gTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGCGTGGTGATTAAAACGATCGGCTTTTTTATCGCCCAAGC‑AGTCTCGGCGCGGTTTGATCATCTCGTGAAAGAAGATACCGGATTTATCGGCTT‑CCAAAATATTGATGCGATTGGTTAAAAACCGTTGGAAGAGaaaatgagcaccacgatgcgg                                                                                     >  2:228664/1‑210 (MQ=255)
                                                                                                                                            tcctcctctgtctcGCCGGTAGGCAACTTGTATGAGTGGTGTGCCAAAGCCTACGACGAAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCGTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTAGCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTga                                                                                 <  2:640314/217‑1 (MQ=255)
                                                                                                                                                 ctctTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGGTTTATCGGCTT‑CAAAAATATGGAGCCGATTGCTGAACATCCGTTGGAAGAGAAACTGGCCGCCCTGCGTCCGCTga‑agga                                                                            >  1:8096/1‑228 (MQ=255)
                                                                                                                                                 ctctTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGGTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTga‑aggaa                                                                           >  1:86343/1‑229 (MQ=255)
                                                                                                                                                        tcGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGGTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAAAATCCGTTGGAAGAGAATCTGGCCGCCCTCCGTCGGCTGA‑AGGAAGATTatc                                                                    >  1:462814/1‑227 (MQ=255)
                                                                                                                                                         cggcggtagggcatttatATGAGATGTGTGGCAAAGCCTACGACTAAGGTG‑GGGGCGGTTGGGTGTTTAAAACGATCGGCTGTTTTATCGCCAACGA‑AGTCTCGCCGCGGTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTAccc                                                                   <  1:334053/212‑1 (MQ=255)
                                                                                                                                                           ccGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGGTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTTATCATCTGGTGAACGAAGATCACGGTTTTATCCGCTT‑CCAAAATAAGGGCGCGATTGCTGTACATCAGTTTGAGGGGAATTTGGGCCACCGGCGCCGGATGT‑AGAAAGagatccccg                                                                 >  2:334053/1‑221 (MQ=255)
                                                                                                                                                             ggTTGGCAACTGCTTTGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGGGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGAc                                                               <  2:198931/229‑1 (MQ=255)
                                                                                                                                                              gttggCAACTGTTTTGAGATGTGTGCCAAAGCCTACGAACCAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTAGCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACa                                                              <  2:19690/226‑1 (MQ=255)
                                                                                                                                                              gTAGGCAACTGCTGGGAGATGTGGTCCAAAGCCTAAGACACAGGTT‑GGGGCGGTTTGGTGTTTAAAACGATCGGCTTTTTTAGCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACa                                                              <  2:489006/229‑1 (MQ=255)
                                                                                                                                                              gTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCCGAGTGCTGAAAATCCGTTGGGAAGGAAACTGGACCCCCTGGCTGGGCTGA‑AGGCAGATTACCCGGAc                                                               >  2:564527/1‑228 (MQ=255)
                                                                                                                                                              gTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACa                                                              <  1:353980/229‑1 (MQ=255)
                                                                                                                                                                tggCAATTGCTTTTAGTTGTGTGCCAAAGCCCACGACACGGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACaaa                                                            <  2:86343/228‑1 (MQ=255)
                                                                                                                                                                            tgtgattggtgCCAAGGCCTTCGACACAGGTT‑GGGGCGGTGTGGTGGTTAAAACGATCGGCTTTTTGATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCAGCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑Ct                                              <  2:408629/221‑1 (MQ=255)
                                                                                                                                                                              agaTGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGCAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAACAGATTTGTGAAAACGCGTTGGAAGGAAACTTGGCCGCCCTGCAGCGGCTGG‑AGGGAAGTTACCCGGGAAAAGAATTTGATCG‑Ctt                                             >  2:454397/1‑229 (MQ=255)
                                                                                                                                                                               gaTGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑CTTc                                            <  1:25060/228‑1 (MQ=255)
                                                                                                                                                                                  tttgcccAAGGCTTTCGAAACTGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑CTTCGA‑Tc                                       <  2:307027/224‑1 (MQ=255)
                                                                                                                                                                                           aGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATTGAGCAGATTGCTGAACATCCCTTGGAAGGGAATCTGGCCGCCCTGCGTCGGCTGA‑GGGAAGATTACCCGGGCAAAAAA‑TTTGACG‑CTTCCG‑TAATGGGGGaa                              >  2:120873/1‑229 (MQ=255)
                                                                                                                                                                                                aCGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGCTACCGGGTTTATCGGCTTCCAAAAAAATGGA‑CAGATTGCTGAACATCCGTTGGAAGGGGATATGGCCGCCCAGCCTCGGGTGA‑AGGAAGATTCGCCGGGCCAAGTG‑TTG‑TCGCCTTCGG‑GCATGGGGGGAAAAGa                         >  2:636506/1‑229 (MQ=255)
                                                                                                                                                                                                aCGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGGGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATTGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTAACCGGACAAAGGA‑TTGATAC‑CTTCGA‑TCATGGGGGaaacaga                         >  1:105231/1‑225 (MQ=255)
                                                                                                                                                                                                  gtctcaGGTT‑GGGGGGGTGTGGGGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑CTTCGA‑TCATGGGGGAAAATGag                        <  1:248480/224‑1 (MQ=255)
                                                                                                                                                                                                   acacAGGTT‑GGGGCTGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑CTTCGA‑TCATGGGGGAAAATGagc                       <  1:534669/228‑1 (MQ=255)
                                                                                                                                                                                                               ggCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CTAAAATATGGAGCAGATTGGTGAACACCCGTTTGAAGGAAAACTGGCCCCCCTGCCTCGGCTCAGAGAAGGATTCCCGGGACAAAG‑A‑TTGATGC‑CCTCGACCCTTGGGGCAAAATgcaagaaagaggcg             >  2:182607/1‑215 (MQ=255)
                                                                                                                                                                                                                    tgtggtggTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGGCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGCTTGATGAACACTCGTTGGAAGAGAATCTGGCCGGCCTGCTGCGGCTGG‑AGGAAAATTACCCGGACAAAATA‑TTGATCC‑GTTTGC‑TCCTGGGGGGAAATGAGCAGGCAAGGGAGGAGCTg      >  2:245635/1‑229 (MQ=255)
                                                                                                                                                                                                                        gtgtTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑CTTCGA‑TCATGGGGGAAAATGAGCAGCAATGGGAGGCGCTGgcgc  >  1:368186/1‑229 (MQ=255)
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         
GGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑CTTCGA‑TCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGC  >  NC_000913/2235180‑2235623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A