Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F97 I2 R1
|
11 |
65.9 |
2309030 |
94.5% |
2182033 |
142.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,160,112 |
G→T |
H226Q (CAC→CAA) |
sthA ← |
pyridine nucleotide transhydrogenase, soluble |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,160,112 | 0 | G | T | 96.7%
| 163.7
/ NA
| 60 | H226Q (CAC→CAA) | sthA | pyridine nucleotide transhydrogenase, soluble |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base T (28/30); total (28/32) |
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATA > NC_000913/4159974‑4160250
|
ggTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGAt < 2:967944/143‑1 (MQ=255)
cGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGga < 2:892931/143‑1 (MQ=255)
gTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCCCTGTTCCCGAATTGATAGgagagag > 1:251519/1‑143 (MQ=255)
tAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTCGTGACGAATCACTACGCCCCTGTTCCAGAAGTGATAGGAGAGAGAATCTGa < 2:603410/143‑1 (MQ=255)
caggcagTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGGCAtct > 1:996416/1‑143 (MQ=255)
ggcagTCAGCTTTCAGTTTATTACCCGACTACAGATGCATGATCACACCATCCTCACAGCCTTCGATATTCTCATACTCTCCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGACAGGAGAGAGAATCtacaatccct > 2:667336/1‑134 (MQ=255)
gTCAGCTTTCAGTTTTTTACCCGACTTAAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGAAATCTATTGaa > 2:466974/1‑142 (MQ=255)
gTCAGCTTTCACTTTCTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGAt < 1:830743/143‑1 (MQ=255)
aGCTTTCAGTTTTTTACCCGACTGCAGATGCATGAGCACACCAGCGTCACAGCCTTCGATCTTCTCGTACGCTTCGTTGGGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCga < 1:472665/143‑1 (MQ=255)
gtgggacgttgttTACCCGTCTGCAGTTGCATGATCGCACCGTCGGCACAGCCTTCGAGCTTCTCGTACTCTTGGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACAGCTCTTGATCtag < 2:231550/136‑1 (MQ=255)
ggTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGc < 2:961848/142‑1 (MQ=255)
aGTTTTTTACCCGACTTCAGATCCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGc > 1:608140/1‑143 (MQ=255)
ttttttCCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCcag < 2:77841/143‑1 (MQ=255)
ttttACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCCCTGTTCCAGAATTGATAGGAGCGAGAATCTGACACCTCTTGCTCGAGCACCGCcagc > 1:443049/1‑143 (MQ=255)
ccGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATCGCAGAGAGAATCTGGCTTCCCTTGATCGAGAAATGCGAGCAGGCGa > 1:98248/1‑143 (MQ=255)
ccGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGa > 2:16693/1‑143 (MQ=255)
cGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGAt < 1:749475/143‑1 (MQ=255)
gACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTAATAGGAGAGAGAATCTGACAGATCTTGATCGAGAAATGCCAGCAGGCGATc > 1:845955/1‑143 (MQ=255)
ttCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATcgcg > 1:55240/1‑143 (MQ=255)
tCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCgg < 1:69635/143‑1 (MQ=255)
tCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCgg < 1:701864/143‑1 (MQ=255)
gATGCGTGATCACACCATCGTCACAGCCTTCGATCTTCTCGTAATCTTTGGTGTGACGAGTCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGgtg < 1:43380/143‑1 (MQ=255)
aTGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGGCACAGTTACAGAATTCATAGGAGAGAGAATCTGACACCTCTTGATCGAGAAATGCCAGCAGGCGGTCGCGGgtgt > 2:624118/1‑143 (MQ=255)
aTGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGGTGAt > 1:254727/1‑143 (MQ=255)
gctCGTCCGAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATGTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACt < 1:417097/141‑1 (MQ=255)
tCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACttt < 1:910936/143‑1 (MQ=255)
tCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACttt < 1:277036/143‑1 (MQ=255)
gcgtgagcgCCTTCGATCTTCGCGTATTCTTCGTTGTGACGAATCACTACGCCTCTGTTCCAGAATTGATAGGCGGGAGATTCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACttt < 2:161954/135‑1 (MQ=255)
cgccccaGCCCTCGATCTTCTCGCTCTCTTCGTTGTGACGAATCACGACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACtttt < 1:144325/138‑1 (MQ=255)
cGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCCGGCGATCGCGGGTGTTGGTCAGATCCACtgtt > 1:617107/1‑143 (MQ=255)
gtggcaGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTa < 1:78309/139‑1 (MQ=255)
cacaGCCGTCGGTGTGCTCGTACTCTTCGTTGGGACGAATCACGACGCCACTGTTCCAGAATGGATAGGCGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCTCTTTTACa < 2:1012805/143‑1 (MQ=255)
caGCCTGCGATCTTCTCGGTCTCTTCGGCGGGGCTGATCCCTACGCCACTGCTCCAGAATTGATAGGAGATAGAATCTGACAGCTCTGGAGCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATc < 2:1041835/143‑1 (MQ=255)
cTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGTGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATa < 2:352112/143‑1 (MQ=255)
ttCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCGGAATTGATAGGAGAGGGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCGGAGCGACTGTTACAGCCATAc > 1:445547/1‑143 (MQ=255)
ttCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATAc > 2:1137859/1‑143 (MQ=255)
ttCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGGTGATCAGATCCCCTTTTACATCCAtgc > 1:563106/1‑141 (MQ=255)
ttCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGACAATCTGACATCTCTTGATCGAGGAATGCCAGCAGGTGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATAc > 2:580793/1‑143 (MQ=255)
tCGATCTTCTTGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATAcc > 1:559746/1‑143 (MQ=255)
ctcGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCCGATCCACTTTTACATCCATACCGCGGAAgg > 1:204165/1‑142 (MQ=255)
cGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGACCCACTTTTACATCCATACCGCGGAAGCTc > 1:446228/1‑143 (MQ=255)
tctTCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGc < 1:91690/143‑1 (MQ=255)
ttCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCat > 2:193426/1‑143 (MQ=255)
ttCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCAACTTTTACATCCATACAGCGGAAGATCGACGCat > 2:573323/1‑143 (MQ=255)
tCGTTGTGACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGAGTGTAGATCAGATACACTAATACAACAATACCGCGGAAGATCGACGCata > 1:1054296/1‑143 (MQ=255)
tCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGCGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCata < 2:520133/143‑1 (MQ=255)
tgACGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATAGAGAAATGCAAGCAGGCGATCGCGGGTGTTGATCAGAACAACTTTTGCATCCATACCGCGGAAGATCGAAGCATAttacca > 2:340281/1‑139 (MQ=255)
gcGAATCACTACGCCCCTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGc < 2:996416/142‑1 (MQ=255)
aCGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGc < 2:579585/143‑1 (MQ=255)
aCGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGc < 1:812338/143‑1 (MQ=255)
cGAATCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGcc < 1:1103938/143‑1 (MQ=255)
aaTCACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCATGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGa < 2:743012/143‑1 (MQ=255)
gcACTACGTCACTGTTCCAGAATTGATAGGACAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGCTGATCAGATCCACTTTTCCCTCCATACCGCGGAAGATCGACGCATATTCACAGCCGATc < 1:231834/142‑1 (MQ=255)
cACTACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCa < 1:16693/143‑1 (MQ=255)
tACGCCACTGTTCCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGCTCACTc > 2:1039690/1‑143 (MQ=255)
ccACTGTTTCAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGc < 2:1045983/143‑1 (MQ=255)
tGTTCCATAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCa < 1:1046771/143‑1 (MQ=255)
ccAGAATTGATAGGAGAGAGAATCTGACATCTCTTGATAGAGGAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTGTTACATCCATACCGCGGGAGATCGACGGATATTGACGACCGATCACtacggccccgtgga > 2:631788/1‑130 (MQ=255)
aGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATGCCGCGGAAGATCGAGGCATATTCACAGCGGATCACTCCAGCACCATAGATa > 1:533543/1‑143 (MQ=255)
aGAATTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGaca > 1:994433/1‑141 (MQ=255)
|
GGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATA > NC_000913/4159974‑4160250
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
