Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F121 I1 R2	25	64.0	1407908	95.9%	1350183	136.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,064,016	G→A	A158V (GCA→GTA)	brpA ←	LytR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,064,016	0	G	A	98.1%	178.1 / ‑4.7	53	A158V (GCA→GTA)	brpA	LytR family transcriptional regulator
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (22/29); total (23/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATGCTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAGGAAT  >  CP000730/1063882‑1064146

aaGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCa                                                                                                                              <  1:388081/141‑1 (MQ=255)

  gCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCata                                                                                                                            >  2:223886/1‑141 (MQ=255)

     tcatcaCCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTg                                                                                                                         <  1:424210/141‑1 (MQ=255)

      catcaCCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCATGCTCATTTCCAGCCTCGACAAATACTTTCATATTGa                                                                                                                        >  2:411278/1‑141 (MQ=255)

       atcaCCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGAc                                                                                                                       <  2:439326/141‑1 (MQ=255)

        tcaCCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGAct                                                                                                                      >  1:461261/1‑141 (MQ=255)

        tcaCCATTTAATTTTTGGTACCCTTTGTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGTct                                                                                                                      <  2:112318/141‑1 (MQ=255)

         caCCATTTAATTTTTGGTACCCTTTTTTTATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGActc                                                                                                                     >  2:385646/1‑141 (MQ=255)

          aCCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGActct                                                                                                                    <  2:608591/141‑1 (MQ=255)

             aTTTAATTTTTGTTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTcac                                                                                                                 <  1:303536/141‑1 (MQ=255)

                tAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACata                                                                                                              >  1:165719/1‑141 (MQ=255)

                tAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACata                                                                                                              >  1:165537/1‑141 (MQ=255)

                 aaTTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACataa                                                                                                             <  1:79123/141‑1 (MQ=255)

                   tttttGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACataata                                                                                                           <  1:417242/141‑1 (MQ=255)

                      ttGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATc                                                                                                        <  1:429324/141‑1 (MQ=255)

                       tGGTACCCTTTTTTTTTTTTTTTCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATTCCACCAAGCTCATTTACAGCCTCGACAAATTCTTTCATATTTACTCTCACATAATAATCa                                                                                                       >  2:471387/1‑141 (MQ=255)

                       tGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCa                                                                                                       >  1:162135/1‑141 (MQ=255)

                             ccTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGt                                                                                                 <  1:32529/141‑1 (MQ=255)

                                 ttttaattttaaTCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACAt                                                                                             <  1:371637/141‑1 (MQ=255)

                                 ttttaattttaaTCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACAt                                                                                             >  1:239069/1‑141 (MQ=255)

                                    taattttaatCTTACCTGTATTATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATGCTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCa                                                                                          <  2:552053/141‑1 (MQ=255)

                                       ttttaatCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACataa                                                                                                             <  1:209630/119‑1 (MQ=255)

                                       ttttaatCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACataa                                                                                                             >  2:209630/1‑119 (MQ=255)

                                       ttttaatCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATgg                                                                                       >  1:371582/1‑141 (MQ=255)

                                         ttaatCTTACCAGTATCATATGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCGCACATAATAATCAACAGGTACATTCAttctg                                                                                     <  2:653870/141‑5 (MQ=255)

                                            atCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCt                                                                                  <  2:496634/141‑1 (MQ=255)

                                                 aCCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTAc                                                                             <  1:446358/141‑1 (MQ=255)

                                                   cAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCg                                                                           <  1:337161/141‑1 (MQ=255)

                                                       atcatcTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATc                                                                       >  1:172001/1‑141 (MQ=255)

                                                        tcatcTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATcc                                                                      >  1:231517/1‑141 (MQ=255)

                                                               tgtTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAGCAGGTACATTCATGCTAGCTTCTACCGAATCCCTTGCgg                                                               <  2:257961/141‑1 (MQ=255)

                                                                 ttAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCa                                                             >  1:395342/1‑141 (MQ=255)

                                                                   aGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATGATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAAt                                                           <  2:364934/141‑1 (MQ=255)

                                                                     gTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTg                                                         <  2:461261/141‑1 (MQ=255)

                                                                      ttCATTTAAGTCGTATGGCACATCATAGTATATGCCACAAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTgg                                                        <  1:536469/141‑1 (MQ=255)

                                                                        cATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGac                                                      <  2:657222/141‑1 (MQ=255)

                                                                          tttAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGacca                                                    >  2:264369/1‑141 (MQ=255)

                                                                              aGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCat                                                >  2:259803/1‑141 (MQ=255)

                                                                                 cGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATAtg                                             <  1:467560/141‑1 (MQ=255)

                                                                                   tATGGCACATCATATTATATGCCACCAAGCTCATTTACAGCCACGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATAtgca                                           <  2:264481/141‑1 (MQ=255)

                                                                                         acaTCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGAGCCACCAGAtgcatgtgca                                     >  1:393161/1‑141 (MQ=255)

                                                                                              atAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGt                                <  1:674605/141‑1 (MQ=255)

                                                                                                 gTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAAt                             >  2:367712/1‑141 (MQ=255)

                                                                                                      tGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTAt                        >  2:394156/1‑141 (MQ=255)

                                                                                                              aGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGa                                                       <  2:306211/102‑1 (MQ=255)

                                                                                                              aGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGa                                                       >  1:306211/1‑102 (MQ=255)

                                                                                                              aGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAg                <  2:269341/141‑1 (MQ=255)

                                                                                                              aGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAg                <  2:64297/141‑1 (MQ=255)

                                                                                                                cTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGcc              >  1:200224/1‑141 (MQ=255)

                                                                                                                cTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGcc              <  2:606962/141‑1 (MQ=255)

                                                                                                                        cAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAg      <  2:656617/141‑1 (MQ=255)

                                                                                                                            cTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAGGAAt  >  1:446235/1‑141 (MQ=255)

                                                                                                                            cTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAGGAAt  >  1:617355/1‑141 (MQ=255)

AAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATGCTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAGGAAT  >  CP000730/1063882‑1064146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A