Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F74 I1 R2	13	29.8	697290	91.1%	635231	133.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,373,558	A→T	M73K (ATG→AAG)	pbuG ←	NCS2 family nucleobase:cation symporter‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,373,558	0	A	T	100.0%	93.4 / NA	29	M73K (ATG→AAG)	pbuG	NCS2 family nucleobase:cation symporter‑2
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (15/14); total (15/14)

ATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCC  >  CP000730/2373424‑2373692

atatACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCTCTGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATa                                                                                                                                  <  2:297160/141‑1 (MQ=255)

 tataCCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAg                                                                                                                                 <  1:20469/141‑1 (MQ=255)

  ataCCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGc                                                                                                                                >  1:267684/1‑141 (MQ=255)

              aaaaCGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAAt                                                                                                                    <  2:226640/141‑1 (MQ=255)

               aaaCGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAAtg                                                                                                                   <  2:29044/141‑1 (MQ=255)

                    ccTGTTACACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTCTTAGCCCCTTGACTAGCGAGCATACAAATgctgct                                                                                                              >  1:55669/1‑141 (MQ=255)

                     cTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTa                                                                                                             <  1:311949/141‑1 (MQ=255)

                         tAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGc                                                                                                         >  1:190911/1‑141 (MQ=255)

                              cAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATgaaaaaa                                                                                                    <  2:217941/141‑7 (MQ=255)

                                aCTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCt                                                                                                  >  2:25279/1‑141 (MQ=255)

                                    gCCAAGGAATACCCATGGTTAACACAACAGTAAATGCACAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTAcca                                                                                              <  2:72230/141‑3 (MQ=255)

                                    gCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACaa                                                                                              <  1:185891/141‑1 (MQ=255)

                                        aGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATAGTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAAt                                                                                          <  1:217619/141‑1 (MQ=255)

                                               cccATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCt                                                                                   >  1:35313/1‑141 (MQ=255)

                                                     gTTAACACGACAGTAAATGCAAAGAATGCATTTAATCCCATGCCTGTTGCTAACGCAATTGGATATTTAGCTATTATCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCATTCGCTACAAAAATggaccaaggtgcc                                                                             <  2:190426/141‑12 (MQ=255)

                                                              acaGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCata                                                                    >  2:328353/1‑141 (MQ=255)

                                                                  tAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATAtctt                                                                <  2:267684/141‑1 (MQ=255)

                                                                    aaTGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATAtcttct                                                              >  2:291450/1‑141 (MQ=255)

                                                                      tGCAAAGAATGCAGTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTCGTCCATTCTCATGTCTTCTGa                                                            >  2:88846/1‑141 (MQ=255)

                                                                       gCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACCAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGAt                                                           <  2:72595/141‑1 (MQ=255)

                                                                        cattctttGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATa                                                          >  2:300784/8‑141 (MQ=255)

                                                                         aaaGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGACAAAACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATAc                                                         <  2:259243/141‑1 (MQ=255)

                                                                                  aTTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAac                                                >  2:138688/1‑141 (MQ=255)

                                                                                        tCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGc                                          >  2:92579/1‑141 (MQ=255)

                                                                                           cATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTaa                                       >  2:87232/1‑141 (MQ=255)

                                                                                               ccTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACtt                                   <  1:270060/141‑1 (MQ=255)

                                                                                                   gTGCTAACGCAATTGGATATTTAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTaaaa                               >  1:329424/1‑141 (MQ=255)

                                                                                                                      tttAGCTATTAGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTaa            >  2:281173/1‑141 (MQ=255)

                                                                                                                                aGCCCCTTGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAatagtgtat  >  1:319840/1‑135 (MQ=255)

ATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCC  >  CP000730/2373424‑2373692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A