Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A19 F57 I1 R1
|
14 |
36.1 |
829726 |
89.5% |
742604 |
141.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP000730 |
1,774,221 |
G→T |
A27D (GCC→GAC) |
hemA ← |
glutamyl‑tRNA reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 1,774,221 | 0 | G | T | 100.0%
| 50.0
/ NA
| 18 | A27D (GCC→GAC) | hemA | glutamyl‑tRNA reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (5/13); total (5/13) |
TTGAATATAGTAACGACCTGTGTGAATTTGATCAACAACAGCATATACTTCAGTTCGATTACATGTTGATAATATGACATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGGCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATTATAACATAAT > CP000730/1774091‑1774347
|
acgAATATAGTACCGACCTGTTTGAATTTGATCAACAACAGCATCTACTTCAGTTCGATTACATGTTGATAATATGACATTTTCTAATATAGATTTAGTTTCAAATAAATCTTCCTAGGTAAGTCGTAAGTCATCctctct < 2:191369/139‑1 (MQ=255)
gAATATAGTAACGACCTGTGTGAATTTGATCAACAACAGCATATACTTCAGTTCGATTACATGTTGATAATATGACATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTaa > 1:111998/1‑141 (MQ=255)
gCATATACTTCAGTTCGATTACATGTTGATAATATGACATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCga > 2:363341/1‑141 (MQ=255)
ttGATAATATGACATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAAt < 2:372433/141‑1 (MQ=255)
atGACATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGa < 2:111998/141‑1 (MQ=255)
atGACATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGa < 1:349803/141‑1 (MQ=255)
aCATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAAc < 1:137061/141‑1 (MQ=255)
aTTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGc < 1:302038/141‑1 (MQ=255)
aTTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGc < 1:225434/141‑1 (MQ=255)
aGTTTCATATAAATCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACaa < 1:272920/141‑1 (MQ=255)
aaTCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAAtatta > 1:9105/1‑141 (MQ=255)
tCTTCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATtata < 2:47698/141‑1 (MQ=255)
ttCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATTATAAc < 2:334578/141‑1 (MQ=255)
ttCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATTATAAc < 2:87862/141‑1 (MQ=255)
ttCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATTATAAc < 2:4359/141‑1 (MQ=255)
tCATGGGCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATTATAACa < 1:11800/141‑1 (MQ=255)
gggCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATTATAACATAAt > 2:202806/1‑141 (MQ=255)
gggCAATTCGTAAGTCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAAAGCCCCCATATATACTAACAATACAAATATTATAACATAAt > 1:327405/1‑141 (MQ=255)
|
TTGAATATAGTAACGACCTGTGTGAATTTGATCAACAACAGCATATACTTCAGTTCGATTACATGTTGATAATATGACATTTTCTAAAATAGATTTAGTTTCATATAAATCTTCATGGGCAATTCGTAAGGCATCATCTCTAAAAGCAACTTGCTCTCTTAGTGCAACATCAGCTGTGCGATGATTTATACTAATTGCAATAAAATGCATTTGAAACGCCCCCATATATACTAACAATACAAATATTATAACATAAT > CP000730/1774091‑1774347
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
