Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F57 I1 R1	14	36.1	829726	89.5%	742604	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,902,155	C→T	A105A (GCC→GCT)	arsB →	ArsB arsenite‑antimonite efflux family transporter, membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,902,155	0	C	T	92.7%	101.2 / 0.5	41	A105A (GCC→GCT)	arsB	ArsB arsenite‑antimonite efflux family transporter, membrane subunit
Reads supporting (aligned to +/- strand): ref base C (3/0); new base T (21/17); total (24/17)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

TTTTGTCTCAGTCATTCTTATTTCATTAATATTAGATAAAGTTGGATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCCATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCTTTTATTATTGCGAGTGGTTTTATAGCTGA  >  CP000730/1902023‑1902289

ttttGTCTCAGTCATTCTTATTTCATTAATATTAGATAAAGTTGGATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTtgc                                                                                                                                >  2:193925/1‑141 (MQ=255)

    gTCTCAGTCATTCTTATTTCATTAATATTAGATAAAGTTGGATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCa                                                                                                                            <  2:403406/141‑1 (MQ=255)

    gTCTCAGTCATTCTTATTTCATTAATATTAGATAAAGTTGGATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCa                                                                                                                            <  2:133276/141‑1 (MQ=255)

    gTCTCAGTCATTCTTATTTCATTAATATTAGATAAAGTTGGATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCa                                                                                                                            <  2:401397/141‑1 (MQ=255)

           tCATTCTTATTTCATTAATATTAGATAAAGTTGGATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCg                                                                                                                     >  1:9830/1‑141 (MQ=255)

              ttCTTATTTCATTAATATTAGATAAAGTTGGATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCaa                                                                                                                  >  2:355318/1‑141 (MQ=255)

                 ttATTTCATTAATATTAGATAAAGTTGGATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAatt                                                                                                               >  2:283300/1‑140 (MQ=255)

                      tCATTAATATTAGATAAAGTTGGATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGgc                                                                                                          >  2:76493/1‑141 (MQ=255)

                        aTTAATATTAGATAAAGTTGGATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGgcgc                                                                                                        <  1:195750/141‑1 (MQ=255)

                                 aGATAAAGTTGGATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAAt                                                                                               <  1:335035/141‑1 (MQ=255)

                                  gATAAAGTTGGATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATc                                                                                              >  1:139901/1‑141 (MQ=255)

                                  gATAAAGTTGGATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATc                                                                                              >  1:259179/1‑141 (MQ=255)

                                  gATAAAGTTGGATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATc                                                                                              >  1:133700/1‑141 (MQ=255)

                                           ggATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCt                                                                                     >  1:193417/1‑141 (MQ=255)

                                                  tCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTAt                                                                              >  2:19923/1‑141 (MQ=255)

                                                  tCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTAt                                                                              >  2:258138/1‑141 (MQ=255)

                                                  tCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTAt                                                                              >  2:150219/1‑141 (MQ=255)

                                                  tCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGATGCTATTGTTGCTGCATTTTTCCCTTTTGGTTGCGCAGCGTTACTCTTAACGGCTGTAGTAt                                                                              >  2:219614/1‑141 (MQ=255)

                                                                  tCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTaaaaa                                                              <  1:224331/141‑1 (MQ=255)

                                                                    acaTGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAata                                                            <  2:9830/141‑1 (MQ=255)

                                                                     catgcttcatgcttCAAAAGGAAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCCATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAatat                                                           >  2:265582/1‑141 (MQ=255)

                                                                             atgcttcaAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTa                                                   >  1:324501/1‑141 (MQ=255)

                                                                             atgcttcaAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTa                                                   >  1:204356/1‑141 (MQ=255)

                                                                                    aaaaGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTTTGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCg                                            <  1:316220/141‑1 (MQ=255)

                                                                                    aaaaGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCg                                            <  1:332697/141‑1 (MQ=255)

                                                                                       aGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGTGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGc                                         >  1:183050/1‑141 (MQ=255)

                                                                                       aGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGc                                         <  2:341720/141‑1 (MQ=255)

                                                                                       aGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGc                                         <  2:270175/141‑1 (MQ=255)

                                                                                           aaTGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCATATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCata                                     >  2:288476/1‑141 (MQ=255)

                                                                                                    aaaaTGTTCGTTTATATCATATTATTGGGTGCCATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCtttt                            >  2:389232/1‑141 (MQ=255)

                                                                                                    aaaaTGTTCGTTTATATCATATTATTGGGTGCCATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCtttt                            >  1:341729/1‑141 (MQ=255)

                                                                                                        tGTTCGTTTATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTAGCACCAGATGGCGCAGCGTTAATATTTACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCTTttatta                        <  1:143798/141‑1 (MQ=255)

                                                                                                              tttATATCATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCTTTTATTATTGCGa                  >  1:224878/1‑141 (MQ=255)

                                                                                                                     cATATTATTGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCTTTTATTATTGCGAGTGGttt           <  2:316766/141‑1 (MQ=255)

                                                                                                                           attGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCTTTTATTATTGCGAGTGGTTTTATAGc     >  2:197761/1‑141 (MQ=255)

                                                                                                                           attGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCTTTTATTATTGCGAGTGGTTTTATAGc     >  1:129081/1‑141 (MQ=255)

                                                                                                                           attGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCTTTTATTATTGCGAGTGGTTTTATAGc     >  1:42629/1‑141 (MQ=255)

                                                                                                                            ttGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCTTTTATTATTGCGAGTGGTTTTATAGCt    <  2:279513/141‑1 (MQ=255)

                                                                                                                            ttGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCTTTTATTATTGCGAGTGGTTTTATAGCt    <  2:275942/141‑1 (MQ=255)

                                                                                                                            ttGGGTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCTTTTATTATTGCGAGTGGTTTTATAGCt    <  2:259647/141‑1 (MQ=255)

                                                                                                                              gggTGCTATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCTTTTATTATTGCGAGTGGTTTTATAGCTGa  <  1:122871/141‑1 (MQ=255)

TTTTGTCTCAGTCATTCTTATTTCATTAATATTAGATAAAGTTGGATTATTCGAATGGTCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATCATATTATTGGGTGCCATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTAACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCTTTTATTATTGCGAGTGGTTTTATAGCTGA  >  CP000730/1902023‑1902289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A