Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F58 I1 R1	11	38.2	843732	93.6%	789733	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,643,957	C→T	A38A (GCG→GCA)	accC ←	biotin carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,643,957	0	C	T	100.0%	148.5 / NA	43	A38A (GCG→GCA)	accC	biotin carboxylase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/24); total (19/24)

CCATAACCCGGATGAACGCCATCACAACCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGCGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCTTTTTCATTATTTCACCTTAAATAACGGTT  >  CP000730/1643826‑1644092

ccATAACCCGGATGAACGCCATCACAACCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTa                                                                                                                                >  2:385434/1‑141 (MQ=255)

   tAACCCGGATGAACGCCATCACAACCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATcc                                                                                                                             <  1:105214/141‑1 (MQ=255)

   tAACCCGGATGAACGCCATCACAACCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATcc                                                                                                                             <  1:105288/141‑1 (MQ=255)

   tAACCCGGATGAACGCCATCACAACCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATcc                                                                                                                             <  1:239757/141‑1 (MQ=255)

             gAACGCCATCACAACCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAAt                                                                                                                   >  1:233984/1‑141 (MQ=255)

                      cacaACCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCaa                                                                                                          <  1:87622/141‑1 (MQ=255)

                      cacaACCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCaa                                                                                                          >  1:201612/1‑141 (MQ=255)

                      cacaACCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCaa                                                                                                          <  1:96877/141‑1 (MQ=255)

                      cacaACCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCaa                                                                                                          <  2:233719/141‑1 (MQ=255)

                      cacaACCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCaa                                                                                                          <  2:236757/141‑1 (MQ=255)

                        caACCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACa                                                                                                        >  1:5348/1‑141 (MQ=255)

                         aaCCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAg                                                                                                       <  1:15144/141‑1 (MQ=255)

                         aaCCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAg                                                                                                       <  2:57106/141‑1 (MQ=255)

                         aaCCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAg                                                                                                       <  1:327803/141‑1 (MQ=255)

                                   aGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGcc                                                                                             <  2:241027/141‑1 (MQ=255)

                                          aTAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCa                                                                                      >  2:244913/1‑141 (MQ=255)

                                                   aTGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAgcg                                                                             >  1:366810/1‑141 (MQ=255)

                                                   aTGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAgcg                                                                             >  2:67068/1‑141 (MQ=255)

                                                     gTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGcgcg                                                                           >  1:157832/1‑141 (MQ=255)

                                                                       tGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGAt                                                         <  1:335955/141‑1 (MQ=255)

                                                                           tCTTTAGACAAAGTGGGACCTACGCAATATACTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCa                                                     >  2:350496/1‑141 (MQ=255)

                                                                                  aCAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGt                                              >  2:219470/1‑141 (MQ=255)

                                                                                  aCAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGt                                              >  2:236648/1‑141 (MQ=255)

                                                                                      aGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGc                                          >  2:222803/1‑141 (MQ=255)

                                                                                         gggACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAAt                                       <  2:91154/141‑1 (MQ=255)

                                                                                          ggACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAAtt                                      >  1:198835/1‑141 (MQ=255)

                                                                                          ggACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAAtt                                      >  2:62163/1‑141 (MQ=255)

                                                                                          ggACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAAtt                                      >  1:234517/1‑141 (MQ=255)

                                                                                                  gCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCtt                              <  1:84427/141‑1 (MQ=255)

                                                                                                  gCAATATGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCtt                              <  1:9103/141‑1 (MQ=255)

                                                                                                   cAATATACTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCttt                             <  1:350496/141‑1 (MQ=255)

                                                                                                        tGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCTTTTTCat                        <  2:146498/141‑1 (MQ=255)

                                                                                                        tGCTTCATCAGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCTTTTTCat                        <  1:317575/141‑1 (MQ=255)

                                                                                                              atcaGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCTTTTTCATTATTTc                  >  2:181071/1‑141 (MQ=255)

                                                                                                              atcaGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCTTTTTCATTATTTc                  >  1:390299/1‑141 (MQ=255)

                                                                                                              atcaGCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCTTTTTCATTATTTc                  >  1:201558/1‑141 (MQ=255)

                                                                                                                  gCAATTTGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCTTTTTCATTATTTCACCt              <  1:222803/141‑1 (MQ=255)

                                                                                                                       ttGAGTATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCTTTTTCATTATTTCACCTTAAAt         <  2:164319/141‑1 (MQ=255)

                                                                                                                            tATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTTCGATTTCACCGCGGTTTGCAATTAAAACCTTTTTCATTATTTCACCTTAAATAACgg    <  1:40544/141‑1 (MQ=255)

                                                                                                                            tATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCTTTTTCATTATTTCACCTTAAATAACgg    <  1:292656/141‑1 (MQ=255)

                                                                                                                            tATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCTTTTTCATTATTTCACCTTAAATAACgg    <  2:120837/141‑1 (MQ=255)

                                                                                                                            tATGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCTTTTTCATTATTTCACCTTAAATAACgg    <  1:68975/141‑1 (MQ=255)

                                                                                                                              tGTAGTGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCTTTTTCATTATTTCACCTTAAATAACGGtt  >  1:366466/1‑141 (MQ=255)

CCATAACCCGGATGAACGCCATCACAACCTGTAGAAGTTGCAATAGATAAGATGTTCGGAATATTTAAATATGAATCTTTAGACAAAGTGGGACCTACGCAATATGCTTCATCAGCAATTTGAGTATGTAGCGCATCTTTATCCCCTTCAGAATAGATTGCAACAGTTTGGATGCCTAAATCACGACAAGCGCGAATAATCCTAACTGCGATTTCACCGCGGTTTGCAATTAAAACCTTTTTCATTATTTCACCTTAAATAACGGTT  >  CP000730/1643826‑1644092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A