Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F58 I1 R1	11	38.2	843732	93.6%	789733	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,744,450	A→T	F74L (TTT→TTA)	apt ←	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,744,450	0	A	T	100.0%	110.2 / NA	34	F74L (TTT→TTA)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (17/17); total (17/17)

CACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAC  >  CP000730/1744330‑1744583

cacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATa                                                                                                                   >  2:113967/1‑141 (MQ=255)

cacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATa                                                                                                                   >  2:71431/1‑141 (MQ=255)

cacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATa                                                                                                                   >  2:1340/1‑141 (MQ=255)

     gTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTa                                                                                                              >  2:102607/1‑141 (MQ=255)

            tGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACa                                                                                                       >  2:74679/1‑141 (MQ=255)

            tGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACa                                                                                                       >  2:74660/1‑141 (MQ=255)

                 tAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCaa                                                                                                  <  1:158255/141‑1 (MQ=255)

                 tAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCaa                                                                                                  <  1:22099/141‑1 (MQ=255)

                 tAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCACCTTCTAGCTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCaa                                                                                                  <  1:300594/141‑1 (MQ=255)

                        aTCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATaaa                                                                                           <  2:205791/141‑1 (MQ=255)

                        aTCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATaaa                                                                                           <  1:136877/141‑1 (MQ=255)

                              gtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCAcg                                                                                     <  2:200379/141‑1 (MQ=255)

                                 cagtgctacaaCATTTGTACCATATTCTAGGTCATACTCATAACGACTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGTTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACgcgc                                                                                  <  2:346378/132‑1 (MQ=255)

                                             aTTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTAc                                                                      <  2:269849/141‑1 (MQ=255)

                                               ttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACaa                                                                    >  2:361291/1‑141 (MQ=255)

                                                      atatTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATc                                                             <  2:105414/141‑1 (MQ=255)

                                                      atatTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATc                                                             <  2:212851/141‑1 (MQ=255)

                                                           cTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCCACAACGATCTCAACAt                                                        >  2:116189/1‑141 (MQ=255)

                                                            tAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtc                                                       >  2:71947/1‑141 (MQ=255)

                                                              ggTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACATCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtctc                                                     >  2:229761/1‑141 (MQ=255)

                                                              ggTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtctc                                                     >  2:133650/1‑141 (MQ=255)

                                                              ggTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtctc                                                     >  2:59242/1‑141 (MQ=255)

                                                                    aCTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGCACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCtt                                               <  1:46055/141‑1 (MQ=255)

                                                                     cTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt                                              <  2:264511/141‑1 (MQ=255)

                                                                               aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt                                    >  1:79224/1‑141 (MQ=255)

                                                                               aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt                                    >  1:388848/1‑141 (MQ=255)

                                                                                     tCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAtt                              <  1:206821/141‑1 (MQ=255)

                                                                                        cGAGGTAATTTCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTa                           <  1:92493/141‑1 (MQ=255)

                                                                                                tttCCCTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGc                   <  1:189641/141‑1 (MQ=255)

                                                                                                   cccTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTATAATTTTATCTGTTGCTTa                >  1:291387/1‑141 (MQ=255)

                                                                                                   cccTTCTTTTCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTAAGGGCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATa                >  1:113847/1‑141 (MQ=255)

                                                                                                         ttttCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCata          <  1:247762/141‑1 (MQ=255)

                                                                                                            tCTAACAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGc       <  1:102607/141‑1 (MQ=255)

                                                                                                                 cAGGTGCTAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAACGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAc  >  1:341582/1‑141 (MQ=255)

CACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAC  >  CP000730/1744330‑1744583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A