Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F74 I1 R1	13	68.8	1581882	91.8%	1452167	133.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	774,414	G→A	G555D (GGT→GAT)	fruA →	PTS family fructose/mannitol (fru) porter component IIABC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	774,414	0	G	A	97.4%	252.8 / ‑1.7	77	G555D (GGT→GAT)	fruA	PTS family fructose/mannitol (fru) porter component IIABC
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (29/46); total (30/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-01

AGGTAATGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGGTATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTGCGCCACATG  >  CP000730/774277‑774551

aGGTAATGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGatat                                                                                                                                        >  2:200334/1‑141 (MQ=255)

  gTAATGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGt                                                                                                                                      <  2:535791/141‑1 (MQ=255)

  gTAATGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGt                                                                                                                                      <  1:651212/141‑1 (MQ=255)

     aTGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCAt                                                                                                                                   >  2:663262/1‑141 (MQ=255)

     aTGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCAt                                                                                                                                   >  1:634167/1‑141 (MQ=255)

         agcaCCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATttat                                                                                                                               <  2:599762/141‑1 (MQ=255)

          gcaCCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATttatt                                                                                                                              >  2:235640/1‑141 (MQ=255)

            aCCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTAc                                                                                                                            >  1:711151/1‑141 (MQ=255)

             ccAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACa                                                                                                                           >  1:696813/1‑141 (MQ=255)

             ccAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACa                                                                                                                           >  1:639822/1‑141 (MQ=255)

              cAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAg                                                                                                                          <  2:573980/141‑1 (MQ=255)

              cAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAg                                                                                                                          >  2:22288/1‑141 (MQ=255)

                  tACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAgg                                                                                                                      <  2:225542/141‑1 (MQ=255)

                   aCAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGt                                                                                                                     >  1:437072/1‑141 (MQ=255)

                    cAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTg                                                                                                                    <  2:442746/141‑1 (MQ=255)

                    cAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTg                                                                                                                    >  2:537566/1‑141 (MQ=255)

                    cAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTg                                                                                                                    >  1:107455/1‑141 (MQ=255)

                    cAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATCTATTACAGAAGGTg                                                                                                                    >  1:492262/1‑141 (MQ=255)

                      gCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCg                                                                                                                  <  1:484836/141‑1 (MQ=255)

                      gCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCg                                                                                                                  <  1:33691/141‑1 (MQ=255)

                       cTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGa                                                                                                                 >  2:25493/1‑141 (MQ=255)

                        tGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGAt                                                                                                                >  1:152936/1‑141 (MQ=255)

                              gATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCAtt                                                                                                          <  2:469877/141‑1 (MQ=255)

                                        aTGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTg                                                                                                  <  2:711849/139‑1 (MQ=255)

                                        aTGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTg                                                                                                  >  1:711849/1‑139 (MQ=255)

                                         tGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCg                                                                                               <  1:25791/141‑1 (MQ=255)

                                         tGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCg                                                                                               <  2:92444/141‑1 (MQ=255)

                                           aTTCCACCGTTAGCAATTGCGACAGCGCTGTTAACTTTTAGACGTAAATTTACACAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCAATTTGCACATGCCGAt                                                                                             >  2:667459/1‑141 (MQ=255)

                                           aTTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGAt                                                                                             <  1:291988/141‑1 (MQ=255)

                                           aTTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGAt                                                                                             <  2:639822/141‑1 (MQ=255)

                                           aTTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTACATTTGCAGCTGCCGAt                                                                                             >  2:667447/1‑141 (MQ=255)

                                                aCCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGGTATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCAtt                                                                                        >  2:273342/1‑141 (MQ=255)

                                                  cGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTAc                                                                                      <  1:38555/141‑1 (MQ=255)

                                                     tAGCACTTGCGACAGCGATGTTGATTTTTAGACGTAAATTTACAAAAGAACAGCGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACgtg                                                                                   <  1:721090/141‑1 (MQ=255)

                                                        cAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTa                                                                                >  2:67646/1‑141 (MQ=255)

                                                         aaTTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTAt                                                                               <  1:366368/141‑1 (MQ=255)

                                                         aaTTGCGACAGCGATGTTAATTTTTAGACGTAAATATACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTAt                                                                               >  2:189733/1‑141 (MQ=255)

                                                           ttGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTTAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTc                                                                             <  1:251753/141‑1 (MQ=255)

                                                               gACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTc                                                                         <  1:683365/141‑1 (MQ=255)

                                                                aCAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCa                                                                        <  2:1650/141‑1 (MQ=255)

                                                                 cAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACTACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCaa                                                                       <  2:376937/141‑1 (MQ=255)

                                                                   gcaaTGTTATATTTTAGTCGTAAATTTACAAAAGAACTACGTGGTTCAATTGTCCCTTACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCGATTTGCAGCTTACGATCCATTACGTGTTATTCCTTcgctg                                                                     <  2:152936/138‑5 (MQ=255)

                                                                   gCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCAGCCGATCCATTACGTGTTATTCCTTCAatg                                                                     <  2:711151/141‑1 (MQ=255)

                                                                       tGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAAtgatga                                                                 <  2:144831/141‑1 (MQ=255)

                                                                       tGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAAtgatga                                                                 <  1:580966/141‑1 (MQ=255)

                                                                       tGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAAtgatga                                                                 <  2:107455/141‑1 (MQ=255)

                                                                        gTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATgatgat                                                                <  1:448895/141‑1 (MQ=255)

                                                                        gTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATgatgat                                                                >  1:534372/1‑141 (MQ=255)

                                                                          tAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTg                                                              >  1:664242/1‑141 (MQ=255)

                                                                            aTTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGt                                                            <  1:657010/141‑1 (MQ=255)

                                                                             tttttAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGtt                                                           <  2:171911/141‑1 (MQ=255)

                                                                              ttttAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTc                                                          >  1:568586/1‑141 (MQ=255)

                                                                                ttAGACGTAAATTTACAAAAGAACAACGTGGTTCCATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAg                                                        <  1:730974/141‑1 (MQ=255)

                                                                                   gACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGta                                                     <  1:235640/141‑1 (MQ=255)

                                                                                    aCGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGtat                                                    >  1:488824/1‑141 (MQ=255)

                                                                                    aCGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGtat                                                    <  1:200969/141‑1 (MQ=255)

                                                                                    aCGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGtat                                                    >  2:366024/1‑141 (MQ=255)

                                                                                                  aaGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTg                                      <  2:534372/141‑1 (MQ=255)

                                                                                                    gAACAACGTGGTTCAATTATTCCAAACTAGGTGAGGGAGATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTACAATGAAGAGTGGTTCAGGTATAGGTGGCGCAATTGCt                                    >  2:201508/1‑141 (MQ=255)

                                                                                                     aacaacGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCtt                                   <  2:696568/141‑1 (MQ=255)

                                                                                                     aacaacGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCtt                                   <  1:153578/141‑1 (MQ=255)

                                                                                                         acGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAgg                               >  2:572958/1‑141 (MQ=255)

                                                                                                          cGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTTCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGc                              <  1:122207/141‑1 (MQ=255)

                                                                                                          cGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGc                              <  1:663262/141‑1 (MQ=255)

                                                                                                                tCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGt                        >  1:559920/1‑141 (MQ=255)

                                                                                                                 cAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGtt                       <  2:122823/141‑1 (MQ=255)

                                                                                                                     tATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACg                   <  2:437436/141‑1 (MQ=255)

                                                                                                                       tCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGaa                 <  2:186987/141‑1 (MQ=255)

                                                                                                                       tCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGaa                 <  1:263963/141‑1 (MQ=255)

                                                                                                                        cccTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAAt                <  2:764139/141‑1 (MQ=255)

                                                                                                                            aaCTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACt            >  1:318773/1‑141 (MQ=255)

                                                                                                                              cTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTgc          <  2:229488/141‑1 (MQ=255)

                                                                                                                               tATGTGATGGGTATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTgcg         <  1:273342/141‑1 (MQ=255)

                                                                                                                               tATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTgcg         <  1:337494/141‑1 (MQ=255)

                                                                                                                                   tgATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTGCGCcac     <  1:36145/141‑1 (MQ=255)

                                                                                                                                   tgATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTGCGCcac     >  2:94318/1‑141 (MQ=255)

                                                                                                                                   tgATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGGTCACGAATTACTGCGCcac     >  1:524843/1‑141 (MQ=255)

                                                                                                                                      tGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTGCGCCACAtg  <  2:314001/141‑1 (MQ=255)

AGGTAATGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGGTATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTGCGCCACATG  >  CP000730/774277‑774551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A