Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F74 I1 R1	13	68.8	1581882	91.8%	1452167	133.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,026,955	G→A	T331I (ACA→ATA)	vraS ←	sensor histidine kinase VraS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,026,955	0	G	A	98.7%	271.3 / ‑4.0	79	T331I (ACA→ATA)	vraS	sensor histidine kinase VraS
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (43/35); total (43/36)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

CCAACTACTTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAA  >  CP000730/2026819‑2027084

ccAACTACTTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATAcc                                                                                                                               <  1:87692/141‑1 (MQ=255)

ccAACTACTTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATAcc                                                                                                                               >  2:777880/1‑141 (MQ=255)

   actactTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAACGGTGCTTTCACCTCGCTACGTATACCTGa                                                                                                                            >  1:614790/1‑141 (MQ=255)

     tactTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAAt                                                                                                                          >  1:531983/1‑141 (MQ=255)

       ctTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCt                                                                                                                        >  2:425034/1‑141 (MQ=255)

                cACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGAt                                                                                                               <  2:660373/141‑1 (MQ=255)

                 aCTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTACGGGTGCTTTCCCCTCGATACGTATACCTGAATCTTGCAAGGATa                                                                                                              >  1:430262/1‑141 (MQ=255)

                 aCTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATa                                                                                                              >  1:478531/1‑141 (MQ=255)

                   tttGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACa                                                                                                            >  1:445397/1‑141 (MQ=255)

                           gatagataACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGaa                                                                                                    >  1:111477/1‑141 (MQ=255)

                            atagataACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAAc                                                                                                   >  2:700556/1‑141 (MQ=255)

                              agataACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGt                                                                                                 <  2:739762/141‑1 (MQ=255)

                                   aCTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCAc                                                                                            <  2:291218/141‑1 (MQ=255)

                                    cTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCAcc                                                                                           >  2:574956/1‑141 (MQ=255)

                                        aaaTTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAAtt                                                                                       >  1:522806/1‑141 (MQ=255)

                                         aaTTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAAttt                                                                                      >  2:132000/1‑141 (MQ=255)

                                         aaTTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAAttt                                                                                      >  1:315458/1‑141 (MQ=255)

                                          aTTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTc                                                                                     >  1:477972/1‑141 (MQ=255)

                                             ccTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCaa                                                                                  <  2:315757/141‑1 (MQ=255)

                                             ccTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCaa                                                                                  >  1:285617/1‑141 (MQ=255)

                                             ccTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTAGTTAAAGTTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCaa                                                                                  >  1:358469/1‑141 (MQ=255)

                                              cTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCaaa                                                                                 >  1:40740/1‑141 (MQ=255)

                                              cTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCaaa                                                                                 >  2:514053/1‑141 (MQ=255)

                                               tataCGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAg                                                                                <  2:511295/141‑1 (MQ=255)

                                               tataCGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAg                                                                                <  1:255135/141‑1 (MQ=255)

                                               tataCGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAg                                                                                <  1:485622/141‑1 (MQ=255)

                                                       ccATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCa                                                                        >  1:59890/1‑141 (MQ=255)

                                                        cATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCAc                                                                       <  2:323809/141‑1 (MQ=255)

                                                          tttCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTg                                                                                                                             >  1:161343/1‑85 (MQ=255)

                                                          tttCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTg                                                                                                                             <  2:161343/85‑1 (MQ=255)

                                                           ttCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCa                                                                    <  1:761149/141‑1 (MQ=255)

                                                            tCATGATCATCCACACACACTACTTTGCTCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATCACTGAACCTGGCATTGCTACAATATGGAACGCTGCACCAATTTCCACAGCTCTTTCACGCat                                                                   <  2:276551/141‑1 (MQ=255)

                                                             cATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGa                                                                                                                            <  2:740834/83‑1 (MQ=255)

                                                             cATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGa                                                                                                                            >  1:740834/1‑83 (MQ=255)

                                                             cATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCata                                                                  >  1:691759/1‑141 (MQ=255)

                                                             cATGATCATCCACAAACAATACTCTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCata                                                                  >  2:124514/1‑141 (MQ=255)

                                                               tGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATAtt                                                                <  1:712085/141‑1 (MQ=255)

                                                                   catcCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTa                                                            >  1:605464/1‑141 (MQ=255)

                                                                   catcCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTa                                                            >  1:144176/1‑141 (MQ=255)

                                                                        acaaacaaTACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTcc                                                       <  2:21612/141‑1 (MQ=255)

                                                                        acaaacaaTACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTcc                                                       <  2:21438/141‑1 (MQ=255)

                                                                        acaaacaaTACCTTACTCGTCATACGAATCATCATTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTcc                                                       <  2:157724/141‑1 (MQ=255)

                                                                             caaTACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAAc                                                  >  1:460715/1‑141 (MQ=255)

                                                                             caaTACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAAc                                                  >  1:387847/1‑141 (MQ=255)

                                                                               aTACTTTAATCGTCATACGAAGCCTCCTTATTTAAAGTTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACtt                                                <  2:669665/141‑1 (MQ=255)

                                                                                tACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCa                                                                    <  2:129752/120‑1 (MQ=255)

                                                                                tACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCa                                                                    >  1:129752/1‑120 (MQ=255)

                                                                                   tttAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGtt                                            <  2:429261/141‑1 (MQ=255)

                                                                                    ttAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTc                                           <  2:87357/141‑1 (MQ=255)

                                                                                      aaTCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTa                                         >  1:681542/1‑141 (MQ=255)

                                                                                       aTCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTaa                                        <  2:267330/141‑1 (MQ=255)

                                                                                           tCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAtttt                                    <  1:769126/141‑1 (MQ=255)

                                                                                            cATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAttttt                                   >  2:476277/1‑141 (MQ=255)

                                                                                            cATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAttttt                                   >  1:56688/1‑141 (MQ=255)

                                                                                            cATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAttttt                                   >  1:167097/1‑141 (MQ=255)

                                                                                            cATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAttttt                                   >  2:308648/1‑141 (MQ=255)

                                                                                            cATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCAAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAttttt                                   >  1:356524/1‑141 (MQ=255)

                                                                                             aTACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTtc                                  <  1:582320/141‑1 (MQ=255)

                                                                                              tACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGACCGTTGCCAGAGTATGACAAGATCTTTTAACTGTTTTTTTAAGTCCATAACTTTCTTCTAATTTTtca                                 >  1:667551/1‑141 (MQ=255)

                                                                                               aCGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACACTATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTtcat                                <  1:32695/141‑1 (MQ=255)

                                                                                                cGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTtcatc                               <  1:514053/141‑1 (MQ=255)

                                                                                                cGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTtcatc                               >  2:501647/1‑141 (MQ=255)

                                                                                                   aTCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAAc                            <  1:147339/141‑1 (MQ=255)

                                                                                                         ctTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTaaa                      <  1:425034/141‑1 (MQ=255)

                                                                                                                aaaGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTa               >  1:100224/1‑141 (MQ=255)

                                                                                                                aaaGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTa               >  1:225127/1‑141 (MQ=255)

                                                                                                                    gTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCAt           <  1:476277/141‑1 (MQ=255)

                                                                                                                      gCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCaa                             <  2:393204/121‑1 (MQ=255)

                                                                                                                      gCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCaa                             >  1:393204/1‑121 (MQ=255)

                                                                                                                      gCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTa         >  2:185116/1‑141 (MQ=255)

                                                                                                                      gCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTa         >  1:316195/1‑141 (MQ=255)

                                                                                                                         ttCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCt      <  1:315123/141‑1 (MQ=255)

                                                                                                                         ttCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCt      <  2:527779/141‑1 (MQ=255)

                                                                                                                          ccACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCtt     <  2:430262/140‑1 (MQ=255)

                                                                                                                           cACGTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTg    <  2:233735/141‑1 (MQ=255)

                                                                                                                           cACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTg    <  1:423367/141‑1 (MQ=255)

                                                                                                                           cACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTg    <  2:285617/141‑1 (MQ=255)

                                                                                                                             ccTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGaa  >  2:684302/1‑141 (MQ=255)

                                                                                                                             ccTCGATACGTATACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGaa  >  2:555767/1‑141 (MQ=255)

CCAACTACTTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAA  >  CP000730/2026819‑2027084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A