Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F42 I1 R1	11	27.7	610674	93.3%	569758	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,744,292	A→T	I127N (ATT→AAT)	apt ←	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,744,292	0	A	T	100.0%	79.3 / NA	25	I127N (ATT→AAT)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (12/13); total (12/13)

ATTCGTCGTATGAGATTAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATT  >  CP000730/1744155‑1744427

attCGTCGTATGAGATTAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCg                                                                                                                                      <  1:68662/141‑1 (MQ=255)

   cgtcgtATGAGATTAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTCATTTTTCACCTAATTTTATTGCTGCTTCATTCGTac                                                                                                                                   >  2:253557/1‑141 (MQ=255)

       gtATGAGATTAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTaccacc                                                                                                                               <  1:71300/141‑1 (MQ=255)

       gtATGAGATTAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTaccacc                                                                                                                               <  1:299551/141‑1 (MQ=255)

       gtATGAGATTAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTaccacc                                                                                                                               <  1:286508/141‑1 (MQ=255)

               ttAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCta                                                                                                                       <  2:266172/141‑1 (MQ=255)

                tAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCtaa                                                                                                                      <  1:7037/141‑1 (MQ=255)

                                                  aTACCATTTAAATATTTCAATTAAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGt                                                                                    >  1:71160/1‑141 (MQ=255)

                                                     ccATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTACTTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTa                                                                                 <  1:82374/141‑1 (MQ=255)

                                                         ttAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCGCCTGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTg                                                                             <  2:224920/141‑1 (MQ=255)

                                                          tAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGc                                                                            >  2:84015/1‑141 (MQ=255)

                                                          tAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATCCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTTGTTTAATCGc                                                                            >  2:120288/1‑141 (MQ=255)

                                                                 ttcaattcaaTTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATATATACTTAACACACGTTGACCTGGTTTAATTGCATCTTtg                                                                     >  1:240241/1‑141 (MQ=255)

                                                                 ttcaattcaaTTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAAAAAATAATCTGTAATTAAAACACCATGACCTGGTTTAATTGCATCtttt                                                                     >  1:240232/1‑140 (MQ=255)

                                                                     attcaattATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCa                                                                 >  1:301008/1‑141 (MQ=255)

                                                                                       tACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTAc                                               <  2:274392/141‑1 (MQ=255)

                                                                                        aCCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTAcc                                              <  1:187546/141‑1 (MQ=255)

                                                                                        aCCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTAcc                                              >  2:9145/1‑141 (MQ=255)

                                                                                        aCCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTAcc                                              >  1:277272/1‑141 (MQ=255)

                                                                                         ccTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCa                                             >  1:215420/1‑141 (MQ=255)

                                                                                              tacGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTc                                        <  2:139937/141‑1 (MQ=255)

                                                                                                                  cAACTTATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGaa                    >  2:58965/1‑141 (MQ=255)

                                                                                                                  cAACTAATTTTATTGCTGCTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGaa                    >  2:141057/1‑141 (MQ=255)

                                                                                                                               tgctgcTTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAgg       <  2:301008/141‑1 (MQ=255)

                                                                                                                                    ctTCATTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAtt  <  2:71160/141‑1 (MQ=255)

ATTCGTCGTATGAGATTAAACTCATAACATCGTAATCTTTAATTTTTTCAATACCATTTAAATATTTCAATTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATT  >  CP000730/1744155‑1744427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A