Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F57 I0 R1
|
50 |
10.6 |
235668 |
93.0% |
219171 |
141.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP000730 |
2,373,835 |
C→T |
100% |
intergenic (‑60/+53) |
pbuG ← / ← topB |
NCS2 family nucleobase:cation symporter‑2/DNA topoisomerase TopB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 2,373,835 | 0 | C | T | 100.0%
| 23.1
/ NA
| 8 | intergenic (‑60/+53) | pbuG/topB | NCS2 family nucleobase:cation symporter‑2/DNA topoisomerase TopB |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (3/5); total (3/5) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGAAATAGTTTTTCACGAATGGATGCTCCTTTTTTTAAAATACCTTAATAGTTTAATATCATTCTTAATAAAAAACAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCATCTTTAAACGGATTATTGTCTAAACCTTCATTTTTATTCATATACTTTTTCATTTC > CP000730/2373706‑2373968
|
ttGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGAAATAGTTTTTCACGAATGGATGCTCCTTTTTTTAAAATACCTTACTAGTTTAATATCATTCTTAATAAAAAATAATACCAAAAg > 1:86811/1‑141 (MQ=255)
gTTTGTTCCATATTTATCGAACTGGAAATAGTTTTTCACGAATGGATGCTCCTTTTTTTAAAATACCTTAATAGTTTAATATCATTCTTAATAAAAAATAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGt < 2:50367/141‑1 (MQ=255)
tCACGAATGGATGCTCCTTTTTTTAAAATACCTTAATAGTTTAATATCATTCTTAATAAAAAATAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCa > 2:36771/1‑141 (MQ=255)
ccTTTTTTTAAAATACCTTAATAGTTTAATATCATTCTTAATAAAAAATAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCATCTTTAAACGGatta > 2:114154/1‑141 (MQ=255)
ttttAAAATACCTTAATAGTTTAATATCATTCTTAATAAAAAATAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCATCTTTAAACGGATTATTGTc < 2:86811/141‑1 (MQ=255)
tACCTTAATAGTTTAATATCATTCTTAATAAAAAATAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCATCTTTAAACGGATTATTGTCTAAACCtt < 2:33727/141‑1 (MQ=255)
aaTAGTTTAATATCATTCTTAATAAAAAATAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCATCTTTAAACGGATTATTGTCTAAACCTTCAtttt < 2:46836/141‑1 (MQ=255)
taaAAAATAATACCAAAAGCGAGCTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCATCTTTAAACGGATTATTGTCTAAACCTTCATGTTTATTCATATACTTTTTCATTTc < 2:3412/141‑1 (MQ=255)
|
TTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGAAATAGTTTTTCACGAATGGATGCTCCTTTTTTTAAAATACCTTAATAGTTTAATATCATTCTTAATAAAAAACAATACCAAAAGCGAACTTTTCGTTTTTCTGATTCAACTTTGTTCGATTTTATCTATAAATTCAAGTTCTTTAATGCATCTTTAAACGGATTATTGTCTAAACCTTCATTTTTATTCATATACTTTTTCATTTC > CP000730/2373706‑2373968
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
